Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling

2020 ◽  
pp. 089033442093257
Author(s):  
Sandra Fernández-Pastor ◽  
Dolores Silvestre Castelló ◽  
M.C. López-Mendoza

Background Neonatal infections with Cronobacter sakazakii have recently been associated with the consumption of expressed human milk. Study Aims (1) To evaluate whether human milk has antimicrobial capacity against C. sakazakii and (2) to determine the stability of its capacity when it is subjected to various treatments. Methods The antimicrobial capacity of human milk against C. sakazakii was evaluated using an observational, cross-sectional, comparative design. Mature human milk samples ( N = 29) were subjected to different treatments. After incubation at 37°C for 72 hr, samples were compared with fresh milk on the stability of their antimicrobial capacity. Two-way analysis of variance (ANOVA) was performed. Results In fresh milk, counts of C. sakazakii were reduced by 47.26% ( SD = 6.74) compared to controls. In treated milk, reductions were: refrigeration at 4°C for 72 hr ( M = 33.84, SD = 13.84), freezing at –20°C for 1, 2, and 3 months ( M = 40.31, SD = 9.10; M = 35.96, SD = 9.39; M = 26.20, SD = 13.55, respectively), Holder pasteurization ( M = 23.56, SD = 15.61), and human milk bank treatment with ( M = 14.37, SD = 18.02) and without bovine fortifier ( M = 3.70, SD = 23.83). There were significant differences ( p < .05) between fresh and treated milk. Conclusions Human milk has antimicrobial capacity against C. sakazakii. However, its capacity is negatively influenced by common preservation and hygienization methods. Milk should be stored refrigerated for a maximum of 72 hr or frozen for a short period of time.

2021 ◽  
Vol 21 (1) ◽  
pp. 251-259
Author(s):  
Waleska Lima Alves Simas ◽  
Jaiza Sousa Penha ◽  
Liane Batista da Cruz Soares ◽  
Poliana Pereira Costa Rabêlo ◽  
Bruno Luciano Carneiro Alves de Oliveira ◽  
...  

Abstract Objectives: to verify the prevalence and factors associated with maternal insecurity in breastfeeding with lactating women treated at a milk bank. Methods: cross-sectional study with secondary record data from January 2017 to December 2018 at the Human Milk Bank of the University Hospital of the Federal University of Maranhão. Results: a total of 891 lactating women were analyzed, of which 23.4% had maternal insecurity, being the second most prevalent complication. Most were young adults, married (68%), with complete higher education (64.9%) and family income above five minimum wages (32.5%). Regarding the obstetric history, the highest frequency was ofprimigravida (63.7%o), primiparous (70%), who had had seven or more prenatal appointments (91 %) in private health services (76.5%) where most births also occurred (78.6%), with cesarean section being the most prevalent mode of delivery (86.5%), 47.2% reported never having received guidance on breastfeeding and 80.7% were breastfeeding for the first time. There was a statistically significant relationship (p-value < 0.05) of maternal insecurity with the place where the prenatal care was performed and the place and mode of delivery. Conclusion: maternal insecurity was the second most prevalent breast complication among lactating women in the service. It stands out the need for adequate guidance and counseling on the subject, especially during prenatal care, contributing to avoid early weaning


Author(s):  
Maria Berenice Gomes Nascimento ◽  
Lourdes Conceição Martins ◽  
Patricia Ferrante Draghi ◽  
Fernando Luiz Affonso Fonseca

Background: Premature babies have physiological vulnerabilities that influence their absorption and digestion capacity, compromising their clinical condition. Human milk from human milk banks, has contributed enormously to providing these newborns with more complete nutrition. Objective: To evaluate the quality of human milk collected at the Human Milk Bank during its distribution in neonatal units. Methods: Cross-sectional study, with a quantitative approach, carried out in a maternity hospital with Human Milk Bank. After the pasteurization of milk and its consequent release to these units, a new test was carried out in the analyzes of acid titration, caloric and microbiological content. Results and Discussion: Human Milk showed a decrease of approximately 20% in caloric content and a reduction in acidity. Approximately 12.3% of the samples were contaminated by fecal coliforms. Conclusion: There is a need for a second moment of evaluation of human milk, after the flow, or to rethink the new ways of preserving the initial quality of human milk.


Author(s):  
Claudia Tavares de Melo ◽  
Marta Bellazzi Padrão

Objetivo: Relatar a experiência das enfermeiras do banco de leite humano (BLH) da Santa Casa de Misericórdia de São Paulo, demonstrar as novas atribuições, descrever as repercussões destas mudanças e avaliar o volume de leite coletado. Método: Estudo transversal, descritivo e analítico. Os dados foram coletados dos arquivos de registros do BLH desta instituição, de 2016 a 2018. Resultado: Após início de um projeto com atividades educativas voltadas à divulgação da atuação do BLH, observamos o aumento do número de doadoras de leite materno e, consequentemente, melhoria no fornecimento de leite humano pasteurizado aos prematuros internados. No ano de 2016 os atendimentos somaram 2821 mães, e em 2018, 3238 (demonstrando um aumento de 15% no período). No tocante ao volume de leite coletado, foram 278,65 litros em 2016, contra 370,91 litros em 2018; demonstrando um aumento de 33%. Conclusão: As ações de acolhimento, marketing, socialização e busca ativa realizadas favoreceram o aumento do número de doadoras de leite materno e no volume de leite coletado, bem como sua fidelização. A ampliação das atividades levou a um importante espaço de atuação do enfermeiro que trabalha no BLH. Palavras-chave: Aleitamento materno, Banco de leite, Enfermagem obstétricaABSTRACT Objective: To report an experience lived by the nurses of the human milk bank (HMB) of Santa Casa de Misericórdia de São Paulo, demonstrate the new attributions, describe the repercussions of these changes and evaluate the amount of appointments and milk volume collected from 2016 to 2018. Method: It was a cross-sectional descriptive and analytical study. Results: After beginning a project with educational activities aimed disseminating the HMB, culminating in the increase in the number of breast milk donors and, consequently, in the milk provision (more regular) for hospitalized premature newborns. The total number of attendances jumped from 2821 in 2016 to 3238 in 2018 (an increase of 15%). Regarding the volume of collected milk, we got 278,65 liters in 2016 against 370,91 liters in 2018 (an increase of 33 %). Conclusion: The actions of reception, marketing, socialization and the active search performed favored an increase in the number of breast milk donors and in the volume of collected milk, and to keep their loyalty. The expansion of these activities led to an important new field of nurses activities on the HMB. Key words: Breast feeding, Milk banks, obstetric nursing


2021 ◽  
Vol 43 ◽  
pp. e9-43384
Author(s):  
Rafaela Mara Silva Fonseca ◽  
Lilian Fernandes Arial Ayres ◽  
Luana Cupertino Milagres ◽  
Sylvia Do Carmo Castro Franceschini ◽  
Bruno David Henriques

The superiority of breast milk over milk of other species is already consolidated and there are numerous benefits in the practice of breastfeeding. The Human Milk Bank is a privileged place for the establishment of breastfeeding, as actions to encourage, support and promote breastfeeding are developed. This is a cross-sectional, retrospective study carried out at the only maternity hospital in the city. The sample consisted of mothers and babies assisted by a lactation support program from 2005 to 2016 and also mothers and babies monitored by the human milk bank. It was observed that the assistance of the milk bank was associated with the type of delivery performed (cesarean) (OR: 2.11; 95% CI: 1.03-4.33), having premature children (gestational age) (OR: 3 , 48; 95% CI: 1.52-7.95), higher education (OR: 1.16; 95% CI: 1.03-1.32) and being younger (age) (OR: 0.94; 95% CI %: 0.88-0.99) were the indicators that increased the mother's chance to seek care from the milk bank, regardless of parity and place of residence. The support, protection and promotion of breastfeeding that is carried out in this place, is configured as an important scenario favoring this practice, impacting on the health of the mother-child binomial in the present and in the future.


1988 ◽  
Vol 51 (6) ◽  
pp. 467-470 ◽  
Author(s):  
F. JANE LIN ◽  
HAROLD M. BARNHART ◽  
J. STAN BAILEY ◽  
NELSON A. COX ◽  
RONALD R. EITENMILLER

The bacteriological profiles of human milk samples collected from individual donors under supervised conditions of collection were compared to pooled human milk samples obtained from a commercial human milk bank. Total aerobic counts and total coliform counts of individual donor samples were lower than those of pooled, banked human milk. All of the 200 isolates from ten individual samples were staphylococci with Staphylococcus epidermidis predominating (82%). Only 1% of the isolates was identified as Staphylococcus aureus. Forty-two percent of the 100 isolates from five pooled samples were staphylococci and all of these staphylococci were coagulase-negative. Three percent of the isolates from the pooled samples were Streptococcus faecalis. The remainder (55%) were gram-negative organisms. S. epidermidis was the microorganism that was isolated most frequently from either individual (9 of 10) or pooled (3 of 5) samples.


2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nathália Carolina Tomazelli Crespo ◽  
Rosimere Ferreira Santana ◽  
Valdecyr Herdy Alves ◽  
Audrey Vidal Pereira ◽  
Giovanna Rosario Soanno Marchiori ◽  
...  

Objetivo: conhecer o perfil de Diagnósticos de Enfermagem em mulheres nutrizes atendidas no Banco de Leite Humano. Metodologia: estudo descritivo, com 30 mulheres atendidas no Banco de Leite Humano entre doadoras e não doadoras. Os dados foram coletados nos meses de março a maio de 2012. A análise se deu por meio de estatística descritiva. Resultados: os diagnósticos foram descritos para dois grupos: Não doadoras: Disposição para conhecimento melhorado (100,0%), Risco de infecção (80,9%), Integridade Tissular prejudicada (80,9%), Padrão de sono prejudicado (76,1%), Conhecimento deficiente (66,6%), Ansiedade (52,3%); Em Doadoras: Padrão de sono prejudicado (77,7%) e Ansiedade (55,5%). Conclusão: a taxonomia diagnóstica encontra-se de acordo com a população estudada. Validou-se a necessidade do uso dessa tecnologia como um sistema padrão de linguagem.Descritores: Bancos de leite; Diagnóstico de enfermagem; Cuidados de enfermagem; Saúde da mulher; Aleitamento materno.NURSING DIAGNOSIS IDENTIFIED IN WOMEN AT THE HUMAN MILK BANKObjective: to know the profile of Nursing Diagnosis in women hosted in the Human Milk Bank. Methodology: descriptive study, with 30 women attended at the Human Milk Bank between donors and no donors. Data were collected from March to May 2012. The analysis was done through simple descriptive statistics. Results: diagnosis were described for two groups: No donors: Readiness for enhanced knowledge (100.0%), Risk for infection (80.9%), Impaired tissue integrity (80.9%), Disturbed sleep pattern (76.1%), Deficient knowledge (66.6%), Anxiety (52.3%); In Donors: Disturbed sleep pattern (77.7%) and Anxiety (55.5%). Conclusion: the diagnostic taxonomy is in agreement with the studied population. The need to use this technology as a standard language system was validated.Descriptors: Milk banks; Nursing diagnosis; Nursing care; Women’s health; Breastfeeding.ENFERMERÍA DE DIAGNÓSTICO IDENTIFICADOS EN MUJERES ASISTIDA EN BANCO DE LECHE HUMANAObjetivo: conocer el perfil de los diagnósticos de enfermería de las mujeres dio la bienvenida al Banco de Leche Humana. Metodology: estúdio descriptivo, con 30 mujeres atendidas enel Banco de Leche Humana entre los donantes y no donantes. Los datos fueron recolectados entre marzo y mayo de 2012. El análisis se realizó utilizando estadísticas descriptivas simples. Resultados: los diagnósticos han sido descritos por dos grupos: No donantes: Disposición para mejorar los conocimientos (100%), Riesgo de infección (80,9%), Deterioro de la integridade cutánea (80,9%), Trastorno del patrón de sueño (76, 1%), Conocimientos deficientes (66,6%), Ansiedad (52,3%); Em Donantes: Trastorno del patrón de sueño (77,7%) y Ansiedad (55,5%). Conclusión: la taxonomía diagnóstica es función de la población estudiada. Han verificado la necesidad de la utilización de esta tecnología como un sistema estándar de lenguaje.Descriptores: Bancos de leche; Diagnóstico de enfermería; Atención de enfermería; Salud de lamujer; Lactancia materna.


2021 ◽  
Author(s):  
Luz M. Iribarne-Durán ◽  
Laura Serrano ◽  
Francisco M. Peinado ◽  
Manuela Peña-Caballero ◽  
Jose Antonio Hurtado ◽  
...  

2016 ◽  
Vol 24 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Wanessa Debôrtoli de Miranda ◽  
Maria Cristina Passos ◽  
Maria Imaculada de Fátima Freitas ◽  
Palmira de Fátima Bonolo

Abstract Human milk banks (HMBs) have difficulty maintaining and increasing their stocks, and the number of women enrolling as members remains low. The present qualitative study, based on social representation theory, aimed to understand women’s representations of the milk donation experience. The data were collected through semi-structured interviews conducted in 2013. In total, 12 women aged 18 to 39 years old participated in the study. Most were primiparous, married or living with a partner, housewives, completed high school, and belonged to lower economic classes. Four categories emerged from the testimonies: human milk donation; representations about human milk banks (HMB); the importance of breastfeeding for the baby and for one's conceptualization as a mother; and prenatal care and donation awareness. The main reasons for donating were representations that value breastfeeding, human milk, and the donation act. These mothers had difficulties donating, but the rewarding feeling, the value of this practice, and the support they received from people important to them helped with the donation. The findings related to the value of the donation and breastfeeding provide ways to effectively encourage and motivate potential donors, achieving comprehensive care starting from the prenatal period.


2018 ◽  
Vol 94 (4) ◽  
pp. 399-403 ◽  
Author(s):  
Mayla S. Borges ◽  
Angela M. de M. Oliveira ◽  
Wallisen T. Hattori ◽  
Vânia O.S. Abdallah
Keyword(s):  

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