Acute Phase Proteins in Experimentally Induced Pregnancy Toxemia in Goats

The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by SDS-PAGE electrophoresis. The acute phase proteins (APP) identified were Immunoglobulin-A, ceruloplasmin, transferrin, albumin, α1-antitrypsin, heavy and light chains of immunoglobulin-G, haptoglobin, α1-acid glycoprotein and a still unnamed protein, which was called P24. There was no difference (P>0.3) in protein levels among groups, although a significant difference (P>0.05) was observed between distinct experimental moments in each group evidencing a higher response of the APP in the obstructed groups. The APP fractioning of the peritoneal liquid was standardized to establish a standard curve for healthy equines and those submitted to induced intestinal obstruction. Moreover, it was verified that the SDS-PAGE electrophoresis was sensitive and effective to help diagnose abdominal inflammatory processes.

Download Full-text

Haptoglobin and serum amyloid A in milk and serum during acute and chronic experimentally induced Staphylococcus aureus mastitis

Journal of Dairy Research ◽

10.1017/s0022029903006484 ◽

2003 ◽

Vol 70 (4) ◽

pp. 379-386 ◽

Cited By ~ 85

Author(s):

Ulrika Grönlund ◽

Cecilia Hultén ◽

Peter D. Eckersall ◽

Caroline Hogarth ◽

Karin Persson Waller

Keyword(s):

Staphylococcus Aureus ◽

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Chronic Phase ◽

Subclinical Mastitis ◽

Serum Amyloid ◽

Amyloid A ◽

Healthy Control ◽

Experimentally Induced

Local and systemic changes in the acute phase proteins, haptoglobin and serum amyloid A (SAA), were studied in six dairy cows during the acute and chronic phases of experimentally induced Staphylococcus aureus mastitis. Haptoglobin and SAA were measured in serum, and in milk from infected and healthy control udder quarters within each cow. Concentrations of haptoglobin and SAA increased rapidly in both serum and milk during the acute phase of mastitis and followed a similar pattern. Significantly raised milk concentrations of SAA were also found during chronic subclinical mastitis. Serum concentrations of SAA also tended to be higher during the chronic phase than pre-infection. Increases in milk haptoglobin and SAA were specific for the infected udder quarters. In conclusion, measurement of SAA in milk samples could be a useful tool in diagnosing mastitis.

Download Full-text

Acute-phase proteins in gingival crevicular fluid during experimentally induced gingivitis

Journal of Periodontal Research ◽

10.1111/j.1600-0765.1994.tb01213.x ◽

1994 ◽

Vol 29 (3) ◽

pp. 196-202 ◽

Cited By ~ 23

Author(s):

E. Adonogianaki ◽

N. A. Moughal ◽

J. Mooney ◽

D. R. Stirrups ◽

D. F. Kinane

Keyword(s):

Acute Phase ◽

Gingival Crevicular Fluid ◽

Acute Phase Proteins ◽

Crevicular Fluid ◽

Experimentally Induced

Download Full-text

The Diagnostic Value of Acute phase proteins in Barki Ewes with Pregnancy Toxemia

Alexandria Journal of Veterinary Sciences ◽

10.5455/ajvs.26377 ◽

2019 ◽

pp. 1

Author(s):

Asmaa Darwish ◽

Iman Ebissy

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Diagnostic Value ◽

Pregnancy Toxemia

Download Full-text

Acute phase proteins and total leukocyte counts in blood of mares with experimentally induced ascending placentitis

Journal of Equine Veterinary Science ◽

10.1016/j.jevs.2013.10.155 ◽

2014 ◽

Vol 34 (1) ◽

pp. 215 ◽

Cited By ~ 1

Author(s):

I.C. Canisso ◽

B.A. Ball ◽

M.H. Troedsson ◽

C. Cray ◽

G.M. Davolli ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Leukocyte Counts ◽

Experimentally Induced

Download Full-text

Keratins 8/18 represent type II acute-phase proteins which are differentially regulated in human liver disease

Zeitschrift für Gastroenterologie ◽

10.1055/s-0032-1331972 ◽

2013 ◽

Vol 51 (01) ◽

Author(s):

N Güldiken ◽

V Usachov ◽

K Levada ◽

M Ziol ◽

P Nahon ◽

...

Keyword(s):

Liver Disease ◽

Acute Phase ◽

Human Liver ◽

Acute Phase Proteins ◽

Type Ii ◽

Human Liver Disease

Download Full-text

Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokines, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers

Poultry Science ◽

10.1016/j.psj.2020.12.011 ◽

2020 ◽

Author(s):

Ali Merzza Humam ◽

Teck Chwen Loh ◽

Hooi Ling Foo ◽

Wan Ibrahim Izuddin ◽

Idrus Zulkifli ◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Heat Shock ◽

Acute Phase ◽

Acute Phase Proteins ◽

Gut Barrier ◽

Expression Levels ◽

Enzymes Activity ◽

Gene Expression Levels

Download Full-text

Comparison of host endothelial, epithelial and inflammatory response in ICU patients with and without COVID-19: a prospective observational cohort study

Critical Care ◽

10.1186/s13054-021-03547-z ◽

2021 ◽

Vol 25 (1) ◽

Author(s):

Pavan K. Bhatraju ◽

Eric D. Morrell ◽

Leila Zelnick ◽

Neha A. Sathe ◽

Xin-Ya Chai ◽

...

Keyword(s):

Endothelial Dysfunction ◽

Epithelial Cell ◽

Acute Phase ◽

Cell Injury ◽

Acute Phase Proteins ◽

Invasive Mechanical Ventilation ◽

Icu Patients ◽

Icu Admission ◽

Amyloid A ◽

Epithelial Cell Injury

Abstract Background Analyses of blood biomarkers involved in the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection can reveal distinct biological pathways and inform development and testing of therapeutics for COVID-19. Our objective was to evaluate host endothelial, epithelial and inflammatory biomarkers in COVID-19. Methods We prospectively enrolled 171 ICU patients, including 78 (46%) patients positive and 93 (54%) negative for SARS-CoV-2 infection from April to September, 2020. We compared 22 plasma biomarkers in blood collected within 24 h and 3 days after ICU admission. Results In critically ill COVID-19 and non-COVID-19 patients, the most common ICU admission diagnoses were respiratory failure or pneumonia, followed by sepsis and other diagnoses. Similar proportions of patients in both groups received invasive mechanical ventilation at the time of study enrollment. COVID-19 and non-COVID-19 patients had similar rates of acute respiratory distress syndrome, severe acute kidney injury, and in-hospital mortality. While concentrations of interleukin 6 and 8 were not different between groups, markers of epithelial cell injury (soluble receptor for advanced glycation end products, sRAGE) and acute phase proteins (serum amyloid A, SAA) were significantly higher in COVID-19 compared to non-COVID-19, adjusting for demographics and APACHE III scores. In contrast, angiopoietin 2:1 (Ang-2:1 ratio) and soluble tumor necrosis factor receptor 1 (sTNFR-1), markers of endothelial dysfunction and inflammation, were significantly lower in COVID-19 (p < 0.002). Ang-2:1 ratio and SAA were associated with mortality only in non-COVID-19 patients. Conclusions These studies demonstrate that, unlike other well-studied causes of critical illness, endothelial dysfunction may not be characteristic of severe COVID-19 early after ICU admission. Pathways resulting in elaboration of acute phase proteins and inducing epithelial cell injury may be promising targets for therapeutics in COVID-19.

Download Full-text

Effects of Stocking and Transport Conditions on Physicochemical Properties of Meat and Acute-Phase Proteins in Cattle

Foods ◽

10.3390/foods10020252 ◽

2021 ◽

Vol 10 (2) ◽

pp. 252

Author(s):

Ahmed A. Abubakar ◽

Idrus Zulkifli ◽

Yong M. Goh ◽

Ubedullah Kaka ◽

Azad B. Sabow ◽

...

Keyword(s):

Physicochemical Properties ◽

Acute Phase ◽

Acute Phase Proteins ◽

Stocking Density ◽

Road Transport ◽

Long Distance ◽

Amyloid A ◽

Tropical Conditions ◽

Meat Samples ◽

Water Holding

This study’s objective was to evaluate the effects of distance and stocking density on physicochemical properties and oxidative stability of meat and acute-phase proteins in Brahman crossbred cattle transported by road under hot and humid tropical conditions. Sixty Brahman crossbred heifers were subjected to road transport from a cattle feedlot farm located in Universiti Putra Malaysia (UPM), Serdang, to a commercial ruminant abattoir in Shah Alam, Selangor. Animals were assigned to long and short distances and high, medium, and low stocking densities. The results revealed that the intensity of response significantly increased in meat samples from animals subjected to long-distance transportation and higher stocking density. Alpha-1-acid glycoprotein and serum amyloid-A values increased considerably and were different from the baseline values recorded at preload. In conclusion, the current results revealed that the color, pH, shear force values, water holding capacity (WHC), glycogen level, and malondilaldehyde assay (MDA) concentrations in meat and acute-phase proteins (APP) were affected by both distances and stocking densities, as evidenced by the significant changes recorded from the parameters above.

Download Full-text