Enteritis in an alpaca (Vicugna pacos) associated with a potentially novel adenovirus

Adenovirus-associated enteritis was diagnosed by histopathology of small intestine in a 2-year-old alpaca ( Vicugna pacos). Electron microscopy confirmed intracytoplasmic and intranuclear adenoviral particles within enterocytes. Nucleic acid was extracted from paraffin-embedded tissue sections, and a pan-adenovirus nested polymerase chain reaction (PCR) assay was employed to target a partial sequence of the polymerase gene. The PCR product (321 bp) was cloned and sequenced. Comparison of the nucleotide sequence against the National Center for Biotechnology Information (NCBI) nucleotide database demonstrated 68% identity with the isolates Canine adenovirus 1 and Bovine adenovirus 3. Comparison of the predicted amino acid sequence against the NCBI database demonstrated 75% identity with Bovine adenovirus 3. Phylogenetic analysis supported the relatively close relationship of this isolate to Bovine adenovirus 3, but the alpaca isolate was sufficiently distant to be considered a potentially novel adenovirus for this species.

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Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/18/1/15267 ◽

2020 ◽

Vol 18 (1) ◽

pp. 75-85

Author(s):

Vu Dinh Duy ◽

Tran Thi Viet Thanh ◽

Phan Ke Loc ◽

Nguyen Minh Tam ◽

Nguyen Thi Thanh Huong ◽

...

Keyword(s):

Success Rate ◽

Genetic Distances ◽

Its Rdna ◽

Dna Barcodes ◽

Phylogenetic Taxonomy ◽

Panax Species ◽

Pcr Product ◽

Matk Gene ◽

Close Relationship ◽

Relationship Of

DNA barcoding is a useful tool for species identification using standardized genomic DNA fragments. We used DNA barcodes (ITS-rDNA and matK gene) to explore Panax (32 samples collected from Phu Xai Lai Leng mountain and 19 samples collected from medicinal nursery of TH), and to investigate the phylogenetic taxonomy of Panax. In this study, the PCR success rate for ITS-rDNA and matK region was 100%. The success rate of bidirectional sequencing of PCR product was 100% of ITS-rDNA and matK region with length of 616 bp, 1433 bp, respectively. All 32 samples (Panax TB) of Phu Xai Lai Leng have a close relationship with P. stipuleanatus (MLBS = 99%, BPP = 100%). All 19 samples (Panax TH) of medicinal nursery have a close relationship with P. notoginseng (MLBS = 100%, BPP = 100%). Interspecific genetic distances within and among Panax species was varied from 0.2% to 7.9%, average (4%) (ITS-rDNA gene) and 0.1 to 2.9%, average (1.2%) (matK gene). The genetic relationship of species/gender belonging to the Panax genus showed that they have the same evolutionary origin and discovered that new distributed of P. stipuleanatus in Phu Xai Lai Leng mountain in Vietnam.

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Detection of herpes simplex virus type 2 Bgl II N fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction

Molecular and Cellular Probes ◽

10.1006/mcpr.1994.1063 ◽

1994 ◽

Vol 8 (6) ◽

pp. 441-447 ◽

Cited By ~ 4

Author(s):

Viraphong Lulitanond ◽

Wasun Chantratita ◽

Kamthorn Thammprasert ◽

Kanchana Nimmanahaeminda ◽

Pornchai Matangkasombut ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Cervical Tissue ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Tissue Sections ◽

Polymerase Chain ◽

Simplex Virus

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Echinococcus granulosus from Mexican pigs is the same strain as that in Polish pigs

Journal of Helminthology ◽

10.1017/s0022149x07787564 ◽

2007 ◽

Vol 81 (3) ◽

pp. 287-292 ◽

Cited By ~ 17

Author(s):

A. Cruz-Reyes ◽

C.C. Constantine ◽

A.C. Boxell ◽

R.P. Hobbs ◽

R.C.A. Thompson

Keyword(s):

Echinococcus Granulosus ◽

Fragment Length Polymorphism ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Taxonomic Status ◽

Distinct Strain ◽

Close Relationship ◽

Sequencing Part ◽

Polymerase Chain ◽

Relationship Of

AbstractSamples of Echinococcus granulosus from seven pigs from Mexico were compared with isolates of the parasite from pigs in Poland and representative strains and species of Echinococcus. Isolates from pigs in Mexico were found to be genetically identical to E. granulosus from Polish pigs and distinct from other major genotypes by sequencing part of the mitochondrial cytochrome c oxidase I (COI) mtDNA locus, restriction fragment length polymorphism (RFLP) of the polymerase chain reaction (PCR) amplified rDNA internal transcribed spacer (ITS) 1 using five different enzymes, and random amplified polymorphic DNA (RAPD) analysis. These results were complemented by data on hook morphology and together strengthen the view that Echinococcus maintained in a cycle involving pigs and dogs is a distinct strain that is conserved genetically in different geographical areas. The present study supports the close relationship of the cervid, camel and pig strains and raises the question of their taxonomic status.

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Disease Severity and Molecular Identification of Banana bunchy top virus, Infecting Local Banana in Bali Island

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.54882 ◽

2020 ◽

Vol 24 (1) ◽

pp. 11

Author(s):

Gusti Ngurah Alit Susanta Wirya ◽

I Putu Sudiarta ◽

Dewa Gede Wiryangga Selangga

Keyword(s):

Sequence Analysis ◽

Disease Severity ◽

Specific Primers ◽

Banana Bunchy Top Virus ◽

Research Activities ◽

Field Samples ◽

Close Relationship ◽

Dna Fragment ◽

Polymerase Chain ◽

Relationship Of

Bunchy top symptoms on banana has been reported in Bali Island since early 2011. Symptoms variation were observed in the field similar to infection of Banana bunchy top virus (BBTV). The identity of the BBTV in Bali on the basis of DNA-S nucleotide sequence has not been studied, therefore research was conducted to identify the species of BBTV infecting local banana in Bali based on sequence analysis. Research activities were initiated by collecting field samples from several local banana growing areas in Bali Island. Incidence of bunchy top disease in all locations reached 8% to 44% with disease severity ranged from 2.6% to 30%. Identification of BBTV from field samples were done by polymerase chain reaction using specific primers for BBTV (CPF/CPR) followed by sequence analysis of amplified DNA target. Specific BBTV DNA fragment was successfully amplified from 10 field samples; sequence analysis of DNA fragments showed their highest homology with BBTV. In addition the phylogenetic analysis confirmed the close relationship of BBTV isolates from Bali with various BBTV isolates from Indonesia and other isolates from the Asian group in GeneBank.

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A PCR-Based Assay for Detection of Fusarium oxysporum f. sp. lactucae in Lettuce Seed

Plant Disease ◽

10.1094/pdis-94-7-0860 ◽

2010 ◽

Vol 94 (7) ◽

pp. 860-866 ◽

Cited By ~ 26

Author(s):

Gladys Chia Y. Mbofung ◽

Barry M. Pryor

Keyword(s):

Fusarium Oxysporum ◽

Intergenic Spacer ◽

Target Sequence ◽

Lettuce Seed ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Intergenic Spacer Region ◽

Pcr Product ◽

Polymerase Chain ◽

Forward Primer

A nested polymerase chain reaction-based (nPCR) assay was developed and evaluated for the rapid detection of Fusarium oxysporum f. sp. lactucae in seed of lettuce. Three primers were designed from sequences of the intergenic spacer region of the rDNA and were used in the PCR amplifications. The first amplification employed the primer pair GYCF1 and GYCR4C and produced a product of 2,270 bp. The second amplification employed the forward primer GYCF1 and the nested primer R943 and produced a single 936-bp PCR product. The nPCR protocol developed successfully detected the target sequence in genomic DNA at 1 fg/μl. A seed assay was tested that included a 4-day incubation step in which seed were maintained under high humidity conditions to increase fungal biomass for DNA extraction. In seed lots prepared by mixing known amounts of F. oxysporum f. sp. lactucae–infested seed with noninfested seed, this assay permitted the detection of the pathogen from lots with infestation rates as low as 0.1%. Samples of lettuce seed obtained from 88 commercial lettuce seed lots were assayed for the pathogen by direct plating and by using the nPCR assay. The pathogen was not detected by either diagnostic method, suggesting the seed lots were pathogen free or the level was below detection limits.

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Bursaphelenchus gillanii sp. n. (Nematoda: Aphelenchoididae) – a new species of the xylophilus group in packaging wood imported from China

Nematology ◽

10.1163/15685411-00002744 ◽

2014 ◽

Vol 16 (1) ◽

pp. 53-62 ◽

Cited By ~ 3

Author(s):

Ute Schönfeld ◽

Helen Braasch ◽

Marko Riedel ◽

Jianfeng Gu

Keyword(s):

New Species ◽

Excretory Pore ◽

Rflp Pattern ◽

Median Line ◽

Female Tail ◽

Pcr Product ◽

Close Relationship ◽

Relationship Of ◽

A New Species

A new Bursaphelenchus species of the xylophilus group was detected in coniferous packaging wood imported with goods from China in 2011. The new species is described herein and compared with other species of the xylophilus group. Bursaphelenchus gillanii sp. n. has a slim body (a = 31 (28-34) and 33 (29-36) in females and males, respectively), c′ = 3.8 (3.2-4.5) and 2.1 (1.7-2.5) in females and males, respectively, a large vulval flap, a 5-7 μm long digitate mucro as a continuation of the female tail, excretory pore at or closely posterior to the median bulb, strongly arcuate spicules, 34 (31-37) μm long as measured along the median line, with prominent pointed rostrum and small cucullus. The ITS-RFLP pattern of the new species obtained by digestion of the PCR product with RsaI, HaeIII, MspI, HinfI and AluI is different from other known Bursaphelenchus species. Results of sequencing the ITS1/2 region demonstrate the close relationship of the new species to B. mucronatus and B. xylophilus.

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Prevalence of canine coronavirus and parvovirus infections in dogs with gastroenteritis in Thailand

Veterinární Medicína ◽

10.17221/5764-vetmed ◽

2012 ◽

Vol 48 (No. 6) ◽

pp. 163-168 ◽

Cited By ~ 9

Author(s):

K. Sakulwira ◽

P. Vanapongtipagorn ◽

A. Theamboonlers ◽

K. Oraveerakul ◽

Y. Poovorawan

Keyword(s):

Polymerase Chain Reaction ◽

Rt Pcr ◽

Chain Reaction ◽

Gene Encoding ◽

Nested Polymerase Chain Reaction ◽

Fecal Samples ◽

Canine Coronavirus ◽

Pcr Product ◽

Causative Agents ◽

Polymerase Chain

Canine coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the causative agents of gastroenteritis in dogs. Seventy fecal samples from dogs with signs of gastroenteritis (vomiting and diarrhea), twenty-five fecal samples from healthy dogs and one CPV-2 vaccine strain were amplified by semi-nested polymerase chain reaction (PCR) and semi-nested reverse transcriptase polymerase chain reaction (RT-PCR), aimed at specifically studying the gene encoding the most abundant capsid protein VP2 of CPV-2 and spike protein of CCV. The specificity of the CCV RT-PCR product was evaluated by sequencing. Positive specimens comprised 44 samples (62.8%) and 9 samples (12.8%) for CPV-2 and CCV, respectively. In nine CCV positive samples, seven displayed co-infection between CCV and CPV-2. Our CCV sequence (AF482001) showed a 94.9% nucleotide identity to CCV reported in GenBank accession number D13096. High prevalence of CCV and CPV-2 infections was found in 1–2 month- and 3–6 month-old dogs, respectively. Molecular biology of these viruses is important primarily for epidemic control and preventive measures.

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An unusual case of spinal cord restricted mycobacteriosis in a European mink

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/s-0038-1623679 ◽

2013 ◽

Vol 41 (01) ◽

pp. 63-66

Author(s):

D. Schaudien ◽

C. Flieshardt ◽

I. Moser ◽

H. Hotzel ◽

A. Tipold ◽

...

Keyword(s):

Spinal Cord ◽

Unusual Case ◽

Histological Evaluation ◽

Mustela Lutreola ◽

Chain Reaction ◽

European Mink ◽

Pcr Product ◽

Granulomatous Lesions ◽

The Central Nervous System ◽

Polymerase Chain

SummaryGranulomatous myelitis due to infection with Mycobacterium avium was diagnosed in a 4-year-old male neutered European mink (Mustela lutreola). The causative agent was detected by an acid-fast stain and further characterized by polymerase chain reaction and DNA sequencing of the PCR product. A thorough histological evaluation of the remaining organs revealed no granulomatous lesions or detectable acid-fast organisms. Although minks are generally highly susceptible for mycobacteria, localised infections, especially of the central nervous system, are unusual and may represent an atypical chronic form of the disease.

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