Effect of Atorvastatin on Wound Healing in Rats

2014 ◽  
Vol 17 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Vanessa Ferraz Suzuki-Banhesse ◽  
Flávia Figueiredo Azevedo ◽  
Eliana Pereira Araujo ◽  
Maria Esméria Corezola do Amaral ◽  
Andrea Moro Caricilli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Glycogen Synthase ◽  
Skin Wound ◽  
Acute Injury ◽  
Lipid Lowering ◽  
Post Injury ◽  
Skin Wound Healing ◽  
Extracellular Signal ◽  
Tnf Α

Skin-wound healing is a complex and dynamic biological process involving inflammation, proliferation, and remodeling. Recent studies have shown that statins are new therapeutical options because of their actions, such as anti-inflammatory and antioxidant activity, on vasodilation, endothelial dysfunction and neoangiogenesis, which are independent of their lipid-lowering action. Our aim was to investigate the effect of atorvastatin on tissue repair after acute injury in healthy animals. Rats were divided into four groups: placebo-treated (P), topical atorvastatin-treated (AT), oral atorvastatin-treated (AO), topical and oral atorvastatin-treated (ATO). Under anesthesia, rats were wounded with an 8-mm punch in the dorsal region. Lesions were photographed on Days 0, 1, 3, 7, 10, 12, and 14 post-injury and samples taken on Days 1, 3, 7, and 14 for protein-expression analysis of insulin receptor substrate (IRS)-1, phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), glycogen synthase kinase (GSK)-3, endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF), extracellular signal-regulated kinase (ERK), interleukin (IL)-10, IL-1β, IL-6, and tumor necrosis factor (TNF)-α. Upon macroscopic examination, we observed significant reductions of lesion areas in groups AT, AO, and ATO compared to the P group. Additionally, AT and AO groups showed increased expression of IRS-1, PI3K, Akt, GSK-3, and IL-10 on Days 1 and 3 when compared with the P group. All atorvastatin-treated groups showed higher expression of IRS-1, PI3K, Akt, GSK-3, IL-10, eNOS, VEGF, and ERK on Day 7. On Days 1, 3, and 7, all atorvastatin-treated groups showed lower expression of IL-6 and TNF-α when compared with the P group. We conclude that atorvastatin accelerated tissue repair of acute lesions in rats and modulated expressions of proteins and cytokines associated with cell-growth pathways.

scholarly journals Adaptive immunity and skin wound healing in amphibian adults

2019 ◽  
Vol 14 (1) ◽  
pp. 420-426 ◽  
Author(s):  
Antonella Franchini
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Tissue Injury ◽  
Skin Wound ◽  
Tissue Response ◽  
Cellular Mechanisms ◽  
Skin Wound Healing ◽  
Whole Process ◽  
Tissue Repair And Regeneration ◽  
Adaptive Immune Cells

AbstractRegeneration and repair with scarring of the skin are two different responses to tissue injury that proceed depending on the animal species. Several studies in multiple organisms have shown that the effectiveness of tissue repair gradually decreases with age in most vertebrates, while the molecular and cellular mechanisms underlying the diverse potentials remain incompletely understood. It is clear, however, that immune system actively participates in the whole process and immune-related activities can mediate both negative and positive roles to influence the quality and diversity of tissue response to damage. Compared with innate immunity, our understanding of the significance of adaptive immune cells in normal repair outcome is limited and deserves further investigation. Here, experimental evidence supporting the contribution of lymphocytes and the involvement of lymphoid organs in skin wound healing are discussed, focusing on the findings emerged in adult amphibians, key animal models for tissue repair and regeneration research.

scholarly journals Tissue repair genes: the TiRe database and its implication for skin wound healing

Oncotarget ◽  
2016 ◽  
Vol 7 (16) ◽  
pp. 21145-21155 ◽  
Author(s):  
Hagai Yanai ◽  
Arie Budovsky ◽  
Robi Tacutu ◽  
Thomer Barzilay ◽  
Amir Abramovich ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Repair Genes

PDGF-BB and TNF-α are involved in delayed skin wound healing in mice exposed to ionizing radiation

2013 ◽  
Vol 33 (9) ◽  
pp. 954-959
Author(s):  
Guo-dong WANG ◽  
Jia-qi WANG ◽  
Yun-fu ZHAO ◽  
Shu-bo BAI ◽  
Xiao-qing CHEN ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ionizing Radiation ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Tnf Α

Hyaluronan in skin wound healing: therapeutic applications

2020 ◽  
Vol 29 (12) ◽  
pp. 782-787
Author(s):  
Ian CC King ◽  
Parviz Sorooshian
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Clinical Practice ◽  
Inflammatory Response ◽  
Tissue Repair ◽  
Therapeutic Potential ◽  
Skin Wound ◽  
Clinical Applications ◽  
Skin Wound Healing ◽  
Proliferative Phase

Hyaluronan is a vital constituent in effective skin wound healing. This polysaccharide is ubiquitous throughout the human body and has functional significance for tissue repair and remodelling. The importance of hyaluronan in the proliferative phase of healing is diverse, impacting on cell migration, proliferation, modification of the inflammatory response and on angiogenesis. As such, it holds therapeutic potential for a variety of clinical applications that range from facilitating effective wound healing to burns management and scarring. This overview of the multifaceted roles of hyaluronan considers its current applications to clinical practice in plastic surgery as well as the latest advances in research.

scholarly journals Oxytocin effects on experimental skin wound healing

2017 ◽  
Vol 2 (4) ◽  
pp. 219-232 ◽  
Author(s):  
Heiko Sorg ◽  
Eberhard Grambow ◽  
Erik Eckl ◽  
Brigitte Vollmar
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Skin Wound ◽  
Skin Tissue ◽  
Major Influence ◽  
Skin Wound Healing ◽  
Therapy Regimen ◽  
Fibroblast Migration ◽  
Dermal Thickness

AbstractObjective:Oxytocin (OXY) has significant effects on mammalian behavior. Next to its role in lactation and social interactions, it is described to support better wound healing as well. However, direct OXY effects on wound healing and the regeneration of the microvascular network are still not clarified. We therefore examined the effects of OXY and an OXY receptor antagonist [atosiban (ATO)] on skin wound healing, focusing on epithelialization and neovascularization.Methods:Skin wound healing has been assessed using intravital fluorescence microscopy in a model of full dermal thickness wounds in the dorsal skin fold chamber of hairless mice. Animals received repetitive low or high doses of OXY or ATO. Morphological and cellular characterization of skin tissue repair was performed by histology and in vitro cell assays.Results:The assessment of skin tissue repair using this therapy regimen showed that OXY and ATO had no major influence on epithelialization, neovascularization, wound cellularity, or inflammation. Moreover, OXY and ATO did neither stimulate nor deteriorate keratinocyte or fibroblast migration and proliferation.Conclusion:In summary, this study is the first to demonstrate that OXY application does not impair skin wound healing or cell behavior. However, until now, the used transmitter system seems not to be clarified in detail, and it might be proposed that it is associated with the stress response of the organism to various stimuli.

scholarly journals Eicosanoids in Skin Wound Healing

2020 ◽  
Vol 21 (22) ◽  
pp. 8435
Author(s):  
Ken Yasukawa ◽  
Toshiaki Okuno ◽  
Takehiko Yokomizo
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Healing Process ◽  
Skin Wound ◽  
Lipid Mediators ◽  
Wound Healing Process ◽  
Biological Functions ◽  
Skin Wound Healing ◽  
External Threats ◽  
Cytokines And Chemokines

Wound healing is an important process in the human body to protect against external threats. A dysregulation at any stage of the wound healing process may result in the development of various intractable ulcers or excessive scar formation. Numerous factors such as growth factors, cytokines, and chemokines are involved in this process and play vital roles in tissue repair. Moreover, recent studies have demonstrated that lipid mediators derived from membrane fatty acids are also involved in the process of wound healing. Among these lipid mediators, we focus on eicosanoids such as prostaglandins, thromboxane, leukotrienes, and specialized pro-resolving mediators, which are produced during wound healing processes and play versatile roles in the process. This review article highlights the roles of eicosanoids on skin wound healing, especially focusing on the biosynthetic pathways and biological functions, i.e., inflammation, proliferation, migration, angiogenesis, remodeling, and scarring.

scholarly journals The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1902
Author(s):  
Ariane Leite Rozza ◽  
Fernando Pereira Beserra ◽  
Ana Júlia Vieira ◽  
Eduardo Oliveira de Souza ◽  
Carlos Alberto Hussni ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Cellular Proliferation ◽  
Antioxidant Defense System ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Ki 67 ◽  
Inflammatory Phase ◽  
Tnf Α

Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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