Analysis of the best roughness surface based on the bearing area curve theory

Author(s):  
Peiyu He ◽  
Suwen Lu ◽  
Yun Wang ◽  
Ruitao Li ◽  
Fuzhu Li

An in-depth study of friction and wear requires analysis of the surface morphologies and contact status of friction pairs in mechanical systems. The bearing area curve is introduced for the calculation of wear, and combined with the concept of repositioning. The application of fast calculation of wear amount and improved three-dimensional characterization of a bearing area curve is achieved by this new method. The best roughness surface is used as the target surface to extract the target bearing area curve. The influence of microtexture parameters on the bearing area curve is studied to determine the optimal microtexture parameters. The test results show that a matching texture surface has a short run-in time and the lowest friction coefficient after stabilization among the surfaces studied herein, and the presence of a texture changes the wear mechanism from the original adhesive wear to abrasive wear and improves the tribological performance. The best texture parameters and laser process parameters are determined, which provide new ideas for the optimal design of surface topography.

Author(s):  
Kotaro Kawai ◽  
Yuki Hirata ◽  
Hiroki Akasaka ◽  
Naoto Ohtake

Abstract Diamond-like carbon (DLC) films have excellent properties such as high hardness, low friction coefficient, high wear resistance, chemical inertness and so on. Because DLC film is considered as an effective coating material to improve their surface properties, this films are used in various applications such as parts for automobiles engines, hard disk surfaces, cutting tools and dies, and so on. DLC films consist of a mixture of sp2 bonded carbon atoms and sp3 bonded carbon atoms. Among them, ta-C film is known as the hardest and strongest film since it mainly consists of sp3 bonded carbon atoms. One of deposition methods to form ta-C is Filtered Cathodic Vacuum Arc (FCVA). The characteristic of this method is that it is possible to remove the droplets and form a high-quality film.. However, even though lots of mechanical components which require ta-C coating have three-dimensionally shapes, it is difficult to coat ta-C film three dimensionally by using FCVA process. At present, researches on 3D deposition of amorphous carbon films on three dimensional components is still insufficient, and investigation reports on the deposition mechanism and characterization of the deposited films are even more limited. In this study, we tried to deposit films on 3D components by the FCVA method and evaluated the microstructure and surface morphologies of films. Although films were coated successfully in the entire surfaces, different properties were showed depending on the location of components. These properties were investigated by Raman spectroscopy and laser microscope.


1976 ◽  
Vol 70 (4) ◽  
pp. 1033-1058 ◽  
Author(s):  
Douglas A. Hibbs

This study focuses on strike activity during the 1950–1969 period in ten industrial societies, The first section of the paper deals with issues of strike measurement and introduces a three-dimensional characterization of strike activity which forms the basis of the subsequent statistical analyses. The next section examines postwar trends in industrial conflict in order to evaluate the argument that strike activity is “withering away” in advanced industrial societies. Time plots of the aggregate volume of industrial conflict show that there has been no general downward movement in strike activity during the postwar period.The third part of the paper develops a number of theoretically plausible statistical models to explain year-to-year fluctuations in the volume of strikes. The empirical results of this section indicate that (1) there is a pronounced inverse relationship between strike activity and the level of unemployment, which suggests that on the whole strikes are timed to capitalize on the strategic advantages of a tight labor market; (2) industrial conflict responds to movements in real wages rather than money wages, which indicates that labor is not misled by a “money illusion”; (3) Labor and Socialist parties are not able to deter strike activity in the short-run despite their strong electoral incentive to do so; and (4) the volume of strikes does seem to be influenced by the relative size of Communist parties, which suggests that such parties remain important agencies for the mobilization of discontent and the crystallization of labor-capital cleavages.


2020 ◽  
Author(s):  
Li Wang ◽  
Xiujuan Zhang ◽  
Xiaoyu Feng ◽  
Chen Duan ◽  
Jiqin Luo ◽  
...  

Abstract Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor with high incidence in East and Southeast Asia. A proper and effective model is urgently needed to explore the mechanisms of tumorigenesis and provide new ideas and methods of cancer treatment.Methods: In this study, we established a three-dimensional (3D) organoid model by patient-derived NPC tissues and adjacent tissues. We described a detailed protocol for culturing of human NPC organoids (including tissue preparation, digestion, culture, subculture, section and immunostaining). The organoids were identified by immunostaining against NPC biomarkers. The drug sensitivity against cisplatin was tested by immunostaining against Ki67 and cleaved Caspase 3.Results: This method was proved to be feasible due to the presence of biomarkers including Vimentin, P63, EGFR and CK34βE12 as well as proliferative/progenitor characteristics such as Ki67, Sox2 and CD54 in NPC organoids. The chemotherapy sensitivity of NPC organoids to cisplatin treatment showed that NPC organoids may be utilized as a platform to verify the effectiveness of different antineoplastic drugs, facilitating to achieve personalized treatment.Conclusions: The NPC organoid may inspire a personalized approach to the management of NPC and expand the repertoire of anti-NPC drugs.


Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.


Author(s):  
J. A. Eades ◽  
A. E. Smith ◽  
D. F. Lynch

It is quite simple (in the transmission electron microscope) to obtain convergent-beam patterns from the surface of a bulk crystal. The beam is focussed onto the surface at near grazing incidence (figure 1) and if the surface is flat the appropriate pattern is obtained in the diffraction plane (figure 2). Such patterns are potentially valuable for the characterization of surfaces just as normal convergent-beam patterns are valuable for the characterization of crystals.There are, however, several important ways in which reflection diffraction from surfaces differs from the more familiar electron diffraction in transmission.GeometryIn reflection diffraction, because of the surface, it is not possible to describe the specimen as periodic in three dimensions, nor is it possible to associate diffraction with a conventional three-dimensional reciprocal lattice.


Author(s):  
D. L. Callahan

Modern polishing, precision machining and microindentation techniques allow the processing and mechanical characterization of ceramics at nanometric scales and within entirely plastic deformation regimes. The mechanical response of most ceramics to such highly constrained contact is not predictable from macroscopic properties and the microstructural deformation patterns have proven difficult to characterize by the application of any individual technique. In this study, TEM techniques of contrast analysis and CBED are combined with stereographic analysis to construct a three-dimensional microstructure deformation map of the surface of a perfectly plastic microindentation on macroscopically brittle aluminum nitride.The bright field image in Figure 1 shows a lg Vickers microindentation contained within a single AlN grain far from any boundaries. High densities of dislocations are evident, particularly near facet edges but are not individually resolvable. The prominent bend contours also indicate the severity of plastic deformation. Figure 2 is a selected area diffraction pattern covering the entire indentation area.


Author(s):  
X. Lin ◽  
X. K. Wang ◽  
V. P. Dravid ◽  
J. B. Ketterson ◽  
R. P. H. Chang

For small curvatures of a graphitic sheet, carbon atoms can maintain their preferred sp2 bonding while allowing the sheet to have various three-dimensional geometries, which may have exotic structural and electronic properties. In addition the fivefold rings will lead to a positive Gaussian curvature in the hexagonal network, and the sevenfold rings cause a negative one. By combining these sevenfold and fivefold rings with sixfold rings, it is possible to construct complicated carbon sp2 networks. Because it is much easier to introduce pentagons and heptagons into the single-layer hexagonal network than into the multilayer network, the complicated morphologies would be more common in the single-layer graphite structures. In this contribution, we report the observation and characterization of a new material of monolayer graphitic structure by electron diffraction, HREM, EELS.The synthesis process used in this study is reported early. We utilized a composite anode of graphite and copper for arc evaporation in helium.


1998 ◽  
Vol 79 (01) ◽  
pp. 104-109 ◽  
Author(s):  
Osamu Takamiya

SummaryMurine monoclonal antibodies (designated hVII-B101/B1, hVIIDC2/D4 and hVII-DC6/3D8) directed against human factor VII (FVII) were prepared and characterized, with more extensive characterization of hVII-B101/B1 that did not bind reduced FVIIa. The immunoglobulin of the three monoclonal antibodies consisted of IgG1. These antibodies did not inhibit procoagulant activities of other vitamin K-dependent coagulation factors except FVII and did not cross-react with proteins in the immunoblotting test. hVII-DC2/D4 recognized the light chain after reduction of FVIIa with 2-mercaptoethanol, and hVIIDC6/3D8 the heavy chain. hVII-B101/B1 bound FVII without Ca2+, and possessed stronger affinity for FVII in the presence of Ca2+. The Kd for hVII-B101/B1 to FVII was 1.75 x 10–10 M in the presence of 5 mM CaCl2. The antibody inhibited the binding of FVII to tissue factor in the presence of Ca2+. hVII-B101/B1 also inhibited the activation of FX by the complex of FVIIa and tissue factor in the presence of Ca2+. Furthermore, immunoblotting revealed that hVII-B101/B1 reacted with non-reduced γ-carboxyglutaminic acid (Gla)-domainless-FVII and/or FVIIa. hVII-B101/B1 showed a similar pattern to that of non-reduced proteolytic fragments of FVII by trypsin with hVII-DC2/D4 on immunoblotting test. hVII-B101/B1 reacted differently with the FVII from the dysfunctional FVII variant, FVII Shinjo, which has a substitution of Gln for Arg at residue 79 in the first epidermal growth factor (1st EGF)-like domain (Takamiya O, et al. Haemosta 25, 89-97,1995) compared with normal FVII, when used as a solid phase-antibody for ELISA by the sandwich method. hVII-B101/B1 did not react with a series of short peptide sequences near position 79 in the first EGF-like domain on the solid-phase support for epitope scanning. These results suggested that the specific epitope of the antibody, hVII-B101/B1, was located in the three-dimensional structure near position 79 in the first EGF-like domain of human FVII.


2018 ◽  
Vol 1 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Kamaljit Singh Boparai ◽  
Rupinder Singh

This study highlights the thermal characterization of ABS-Graphene blended three dimensional (3D) printed functional prototypes by fused deposition modeling (FDM) process. These functional prototypes have some applications as electro-chemical energy storage devices (EESD). Initially, the suitability of ABS-Graphene composite material for FDM applications has been examined by melt flow index (MFI) test. After establishing MFI, the feedstock filament for FDM has been prepared by an extrusion process. The fabricated filament has been used for printing 3D functional prototypes for printing of in-house EESD. The differential scanning calorimeter (DSC) analysis was conducted to understand the effect on glass transition temperature with the inclusion of Graphene (Gr) particles. It has been observed that the reinforced Gr particles act as a thermal reservoir (sink) and enhances its thermal/electrical conductivity. Also, FT-IR spectra realized the structural changes with the inclusion of Gr in ABS matrix. The results are supported by scanning electron microscopy (SEM) based micrographs for understanding the morphological changes.


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