Fistuloates A–C: New antioxidative aromatic compounds isolated from Cassia fistula

Three new aromatic compounds named fistuloates: A (3-butyl-6-ethylnonyl-3-(1-phenylethyl) benzoate), B (3-(6- tert-butyl-1-ethoxy-5-ethyl-4-methylnonyl)-5-ethylbenzoic acid isobutyl ester) and C (4,5-diethoxy-2-propylbenzoic acid 7-(6-pentyltetrahydropyran-2-yl)-heptyl ester) were isolated from the ethyl acetate soluble fraction of Cassia fistula Linn. The structural formulae of the isolated compounds A–C were established through interpretation of their spectral data. Compounds A–C exhibited significant antioxidant properties in 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and superoxide anion radical scavenging assays that signifies that C. fistula carries potential anti-oxidant constituents and may assist to cure diseases associated with oxidative stress and others.

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Note: Antioxidant Properties of Lees Produced during Sake Making Using Fugu Muscle and Fin

Food Science and Technology International ◽

10.1177/1082013203009002008 ◽

2003 ◽

Vol 9 (2) ◽

pp. 123-126 ◽

Cited By ~ 3

Author(s):

T. Nagai

Keyword(s):

Hydroxyl Radical ◽

Food Processing ◽

Superoxide Anion ◽

Anion Radical ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Superoxide Anion Radical ◽

Industrial Wastes ◽

Dpph Radical ◽

Enzymatic Hydrolysates

Enzymatic hydrolysates were prepared from lees produced during sake making using fugu muscle and fin. The antioxidant properties of these hydrolysates were measured using four different methods: autoxidation test, superoxide anion radical, DPPH radical and hydroxyl radical scavenging test. The yields of enzymatic hydrolysates were around 8.0% and their protein average content 0.66 mg/mg. The lees had remarkable antioxidant properties and high superoxide anion radical and hydroxyl radical scavenging abilities in comparison with those of ascorbic acid and α-tocopherol. According to the former results, lees produced during sake making could be used as a protein source with antioxidant properties for different applications in food processing, and its use could contribute to reduce industrial wastes.

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Induction of the superoxide anion radical scavenging capacity of dried ‘funori’ Gloiopeltis furcata by Lactobacillus plantarum S-SU1 fermentation

Food & Function ◽

10.1039/c5fo00668f ◽

2015 ◽

Vol 6 (8) ◽

pp. 2535-2541 ◽

Cited By ~ 13

Author(s):

Takashi Kuda ◽

Maki Nemoto ◽

Miho Kawahara ◽

Satoshi Oshio ◽

Hajime Takahashi ◽

...

Keyword(s):

Superoxide Anion ◽

Anion Radical ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Edible Algae ◽

The North ◽

Radical Scavenging Capacity ◽

Scavenging Capacity ◽

North Eastern ◽

Algal Products

To understand the beneficial properties of edible algae obtained from the north-eastern (Sanriku) Satoumi region of Japan, the antioxidant properties of hot aqueous extract solutions (AES) obtained from 18 dried algal products were determined.

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Effect of extraction conditions of paprika oleoresins on their free radical scavenging and anticancer activity

Open Chemistry ◽

10.2478/s11532-013-0378-1 ◽

2014 ◽

Vol 12 (3) ◽

pp. 377-385 ◽

Cited By ~ 4

Author(s):

Vesna Šaponjac ◽

Dragana Četojević-Simin ◽

Gordana Ćetković ◽

Jasna Čanadanović-Brunet ◽

Sonja Djilas ◽

...

Keyword(s):

Carbon Dioxide ◽

Supercritical Carbon Dioxide ◽

Cell Line ◽

Antiproliferative Activity ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging ◽

Superoxide Anion Radical ◽

Food Ingredients ◽

Supercritical Carbon

AbstractGround spice paprika was extracted with hexane, by conventional Soxhlet procedure (SX oleoresin), and with supercritical carbon dioxide at three different pressures — 20, 30 and 40 MPa (SF20, SF30 and SF40 oleoresins). The effect of extraction method and conditions on the colour intesity of paprika oleoresins, content of α-tocopherol, as well as antioxidant and antiproliferative activity was examined. Hexane showed highest selectivity for paprika pigments (886.02 ASTA), while α-tocopherol showed highest solubility (3846.9 mg kg−1) in supercritical carbon dioxide at 20 MPa. All paprika oleoresins exhibited good superoxide anion radical scavenging activity SF30 being the best superoxide anion radical scavenger. Cell growth activity was evaluated in vitro in human cell lines:cervix epitheloid carcinoma (HeLa), breast adenocarcinoma (MCF7) and colon adenocarcinoma (HT-29). The highest antiproliferative activity was exhibited by SX in MCF7 cell line (IC50=14.28 mg mL−1). Extract SF40 produced significant and selective antiproliferative action towards HeLa cell line. These results suggest that paprika oleoresins, due to high antiradical and tumor cell-inhibiting activity, can be regarded as functional food ingredients.

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Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Scales of Miiuy Croaker (Miichthys Miiuy)

Marine Drugs ◽

10.3390/md16100394 ◽

2018 ◽

Vol 16 (10) ◽

pp. 394 ◽

Cited By ~ 11

Author(s):

Long-Yan Li ◽

Yu-Qin Zhao ◽

Yu He ◽

Chang-Feng Chi ◽

Bin Wang

Keyword(s):

Amino Acid ◽

Hydroxyl Radical ◽

Superoxide Anion ◽

Anion Radical ◽

Antioxidant Properties ◽

Superoxide Anion Radical ◽

Type I ◽

Dpph Radical ◽

Soluble Collagen ◽

Miichthys Miiuy

In this report, acid-soluble collagen (ASC-MC) and pepsin-soluble collagen (PSC-MC) were extracted from the scales of miiuy croaker (Miichthys miiuy) with yields of 0.64 ± 0.07% and 3.87 ± 0.15% of dry weight basis, respectively. ASC-MC and PSC-MC had glycine as the major amino acid with the contents of 341.8 ± 4.2 and 344.5 ± 3.2 residues/1000 residues, respectively. ASC-MC and PSC-MC had lower denaturation temperatures (32.2 °C and 29.0 °C for ASC-MC and PSC-MC, respectively) compared to mammalian collagen due to their low imino acid content (197.6 and 195.2 residues/1000 residues for ASC-MC and PSC-MC, respectively). ASC-MC and PSC-MC were mainly composed of type I collagen on the literatures and results of amino acid composition, SDS-PAGE pattern, ultraviolet (UV) and Fourier-transform infrared spectroscopy (FTIR) spectra. The maximum solubility of ASC-MC and PSC-MC was appeared at pH 1–3 and a sharp decrease in solubility was observed when the NaCl concentration was above 2%. Zeta potential studies indicated that ASC-MC and PSC-MC exhibited a net zero charge at pH 6.66 and 6.81, respectively. Furthermore, the scavenging capabilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical of ASC-MC and PSC-MC were positively correlated with their tested concentration ranged from 0 to 5 mg/mL and PSC-MC showed significantly higher activity than that of ASC-MC at most tested concentrations (p < 0.05). In addition, the scavenging capability of PSC-MC on hydroxyl radical and superoxide anion radical was higher than those of DPPH radical and ABTS radical, which suggested that ASC-SC and PSC-SC might be served as hydroxyl radical and superoxide anion radical scavenger in cosmeceutical products for protecting skins from photoaging and ultraviolet damage.

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In vitro antimicrobial, anti-oxidant properties and GC-MS analysis of the crude methanolic extract and fractions of Solanum dasyphyllum Schumach and Thonn. leaves

10.21203/rs.3.rs-125789/v1 ◽

2020 ◽

Author(s):

Oladapo Elijah Oyinloye ◽

Olumuyiwa. S. Alabi ◽

Olusegun. G. Ademowo

Keyword(s):

Crude Extract ◽

Skin Diseases ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Kinetics Study ◽

Medicinal Uses ◽

Ms Analysis ◽

Soxhlet Apparatus ◽

Anti Oxidant ◽

Time Kill

Abstract Background: Solanum dasyphyllum is a plant with several ethno-medicinal uses as food and medicine in treatment of fever, skin diseases, inflammation, stomach ache and some systemic infections. To date there is little scientific validation of the folkloric claims of S. dasyphyllum compared to other species of the Solanaceae family. This study determined the antimicrobial and antioxidant potential of methanol extract and fractions of the leaves of S. dasyphyllum and analysed the phyto-constituents using GC-MS technique.Methods: The dried leaves of S. dasyphyllum were extracted by Soxhlet apparatus with 80% methanol and the crude extract subjected to Phytochemical screening and liquid-liquid fractionation. Crude extract and fractions were subjected to antimicrobial screening, minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBC/MFC) determination and time-kill kinetics study. Crude extract was assayed for antioxidant activity and analysed by GC-MS.Results: Percentage yield of S. dasyphyllum was 12.5% and tested positive for flavonoids, alkaloids, saponins, cyanogenic glycosides, tannins and reducing sugars. The crude extract and fractions were active on all the isolates at concentrations >0.5 mg/mL. MICs of the crude extract and fractions ranged between 0.25 and >4 mg/mL, MBC ranged between 1 and >4 mg/mL and MFC ranged between 0.5 and >4 mg/mL. The MBC/MIC ratio was >4 for dichloromethane fractions against S. aureus (ratio 8) and MFC/MIC ratio ethylacetate fraction against the fungi isolates (ratio 8). The time-kill kinetics study showed dichloromethane and ethylacetate fractions to be bactericidal with zero viable count at 4 - 6hours against the isolates. The crude extract displayed moderate antioxidant with weak DPPH radical scavenging activity. The GC-MS analysis showed 29 metabolites including the phenols and polyphenols derivatives, eucalyptol, levomenthol, benzofuranone derivative, diethyl phthalate, neophytadiene, 1-Docosene, 17-Pentatriacontene, Phytol, Thunbergol and esters of decanoic acid derivatives all of which are known to have anti-infective and anti-oxidant properties.Conclusion: This study therefore confirmed the ethno-medicinal claim of S. dasyphyllum and thus elucidates the potentials of the plant as a good source of bioactive compounds including those with strong anti-infective and antioxidant properties.

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Antioxidant and ROS inhibitory activities of heterocyclic 2-Aryl-4(3H)-quinazolinone derivatives

Letters in Drug Design & Discovery ◽

10.2174/1570180818666210427092319 ◽

2021 ◽

Vol 18 ◽

Author(s):

Shahida Perveen ◽

Syed Muhammad Saad ◽

Khalid Mohammed Khan ◽

Muhammad Iqbal Choudhary

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Free Radicals ◽

Cell Line ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging ◽

Superoxide Anion Radical ◽

Inhibitory Activities ◽

Quinazolinone Derivatives

Background: Antioxidants are small molecules that prevent or delay the process of oxidations caused by highly reactive free radicals. These molecules are known for their ability to protect various cellular architecture and other biomolecules from oxidative stress and free radicals. Thus, antioxidants play a key role in the prevention of oxidative damages caused by highly reactive free radicals. Method: In the present study, a series of previously synthesized heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 was screened for antioxidant activity by employing in vitro DPPH and superoxide anion radical scavenging activities. ROS inhibitory activities were also evaluated by serum-opsonized zymosan activated whole blood phagocytes and isolated neutrophils. Cytotoxicity studies were carried out by employing an MTT assay against the 3T3 cell line. Results: Most of the 2-aryl-4(3H)-quinazolinone derivatives showed potent antioxidant activities in superoxide anion radical scavenging assay with IC50 value ranging between 0.57 µM – 48.93 µM, as compared to positive control quercetin dihydrate (IC50 = 94.1± 1.1 µM ). Compounds 5, 6, and 14 showed excellent activity in DPPH assay. Compounds 5-8, 12-15, 17, and 20 showed promising activities in the ROS inhibition assay. All compounds were found to be non-cytotoxic against the 3T3 cell line. Structure antioxidant activity has been established. Conclusion: It can be concluded that most of the heterocyclic 2-aryl-4(3H)-quinazolinone derivatives 1-25 are identified as promising antioxidant agents that are capable of fighting against free radicals and oxidative stress. Thus, they can serve as a lead towards treating oxidative stress and related pathologies.

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In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

Nigerian Journal of Natural Products and Medicine ◽

10.4314/njnpm.v25i1.8 ◽

2021 ◽

Vol 25 (1) ◽

pp. 75-79

Author(s):

S.O. Olubodun ◽

G.E. Eriyamremu ◽

M.E. Ayevbuomwan ◽

C.I. Nzoputa

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Vitamin ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Anti Oxidant ◽

In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

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Peripherally substituted soluble nickel phthalocyanines: Synthesis, characterization, aggregation behavior and antioxidant properties

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619501645 ◽

2019 ◽

Vol 23 (11n12) ◽

pp. 1478-1485

Author(s):

Senem Çolak Yazıcı ◽

Sibel Kahraman ◽

Salih Z. Yıldız ◽

Mahmut D. Yılmaz

Keyword(s):

Sulfonic Acid ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Scavenging Activity ◽

The Novel ◽

Diammonium Salt ◽

Ft Ir ◽

Radical Scavenging Ability

Tetra-zwitterionic-substituted nickel(II) phthalocyanine derivatives were newly synthesized starting from nonionic 2(3),9(10),16(17),23(24)-tetrakis-[2-([Formula: see text]-((3-dimethylamino)propyl)carbamate)oxyethyl)phthalocyaninato nickel (II). The novel compounds have been characterized by a combination of UV-vis, FT-IR and mass spectroscopies and elemental analysis. The critical micelle concentrations of the prepared compounds were measured, and the antioxidant activities were analyzed with radical scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The zwitterionic molecules showed aggregated spectra in the UV-vis region, and they might be good surfactant candidates for the detergent industry with their appropriate critical micelle concentration (CMC) properties in water. The compounds exhibited ABTS radical scavenging activity and thus they have antioxidant activity.

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Study on Scavenging Free Radical Activity with Polysaccharides Materials in Chuanxiong Based on Composite Properties of Biomaterials

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.583.244 ◽

2012 ◽

Vol 583 ◽

pp. 244-247

Author(s):

Yong Guang Bi ◽

Chun Chun Liu

Keyword(s):

Free Radical ◽

Superoxide Anion ◽

Anion Radical ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Superoxide Anion Radical ◽

Dose Effect ◽

Spectrophotometric Detection ◽

Free Radical Activity ◽

Composite Properties

By using spectrophotometric detection of the Chuanxiong polysaccharides on the free radical scavenging. The results show that the Chuanxiong polysaccharides on DPPH radical, hydroxyl radical (HO•) and superoxide anion radical (•) clearance. Scavenging ability with the the Chuanxiong polysaccharide concentration increased, and showed the dose-effect relationship. Concentration of 2.0mg/mL when DPPH radicals clear the rate of 35.61%, while the rate of hydroxyl radicals and superoxide anion radical scavenging, clear the rate of 57.78% and 57.14%. Chuanxiong polysaccharide is an ideal natural antioxidants and good prospects for the development of biomedical composites.

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