scholarly journals Chemical Composition of Essential Oil from Flower of ‘Shanzhizi’ (Gardenia jasminoides Ellis) and Involvement of Serotonergic System in Its Anxiolytic Effect

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4702
Author(s):  
Nan Zhang ◽  
Mu Luo ◽  
Lei He ◽  
Lei Yao
Keyword(s):  
Essential Oil ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Serotonergic System ◽  
Control Treatment ◽  
Monoamine Neurotransmitters ◽  
Neurotransmitter Receptor ◽  
Gardenia Jasminoides ◽  
Plus Maze ◽  
Gardenia Jasminoides Ellis

Gardenia jasminoides Ellis is a famous fragrant flower in China. Previous pharmacological research mainly focuses on its fruit. In this study, the essential oil of the flower of ‘Shanzhizi’, which was a major variety for traditional Chinese medicine use, was extracted by hydro distillation and analyzed by GC-MS. Mouse anxiety models included open field, elevated plus maze (EPM), and light and dark box (LDB), which were used to evaluate its anxiolytic effect via inhalation. The involvement of monoamine system was studied by pretreatment with neurotransmitter receptor antagonists WAY100635, flumazenil and sulpiride. The monoamine neurotransmitters contents in the prefrontal cortex (PFC) and hippocampus after aroma inhalation were also analyzed. The results showed that inhalation of G. jasminoides essential oil could significantly elevated the time and entries into open arms in EPM tests and the time explored in the light chamber in LDB tests with no sedative effect. WAY100635 and sulpiride, but not flumazenil, blocked its anxiolytic effect. Inhalation of G. jasminoides essential oil significantly down-regulated the 5-HIAA/5-HT in the PFC and reduced the 5-HIAA content in hippocampus compared to the control treatment. In conclusion, inhalation of gardenia essential oil showed an anxiolytic effect in mice. Monoamine, especially the serotonergic system, was involved in its anxiolytic effect.

GABA and 5-HT Receptor Mediated Anxiolytic Effect of Essential Oil of Ferula sumbul Hook. Roots

2020 ◽  
Vol 10 (3) ◽  
pp. 262-271
Author(s):  
Sonali Batra ◽  
Ashwani Kumar ◽  
Anupam Sharma
Keyword(s):  
Essential Oil ◽  
Receptor Antagonist ◽  
Open Field ◽  
Gaba Receptors ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Anxiolytic Activity ◽  
Ms Analysis ◽  
Significant Reversal ◽  
Plus Maze

Background: Ferula sumbul Hook. (Umbelliferae) roots have been traditionally used as sedative in nervous disorders. Objective: The present study identifies the components of essential oil of F. sumbul roots (EOFS) using GC-MS analysis and further evaluates mechanism-based anxiolytic potential of oil. Materials and Methods: EOFS was extracted using Clevenger apparatus, and was screened for anxiolytic activity using an elevated plus maze model. A battery of models was subsequently used to confirm the anxiolytic potential of EOFS. Further, benzodiazepine (BZD) receptor antagonist flumazenil and pentylenetetrazole (PTZ) were used for investigating the possible involvement of GABA receptors. Results: GC-MS analysis of EOFS revealed the presence of 32 components comprising triterpenoids and their derivatives. The oil exhibited significant anxiolytic activity at 50 μl/kg in various models like the elevated plus maze, light/dark, mirror chamber, open-field and mCPP-induced anxiety. The observation that anxiolytic effect of EOFS was completely blocked by benzodiazepine (BZD) receptor antagonist flumazenil, and partially by pentylenetetrazole, clearly demonstrates that anxiolytic activity of the oil is mediated mainly through BZD site on GABA receptors. Further, a significant reversal of mCPP induced anxiety by EOFS strongly indicates the possible involvement of 5-HT receptors in mediating anxiolytic activity of the oil. Conclusion: Results of the present study clearly demonstrates the anxiolytic potential of EOFS and, thus, validates the traditional relevance of the plant. This is the first report not only on multi-model based antianxiety activity of EOFS but also on the possible antianxiety mechanism of the oil.

scholarly journals Anxiolytic Effect of Two Tobacco Essential Oils (Nicotiana tabacum Linn.) on Mice

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4171
Author(s):  
Danqing Xie ◽  
Lei Yao ◽  
Yan Huang ◽  
Shuaifan Wu ◽  
Li Ma ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Essential Oil ◽  
Essential Oils ◽  
Elevated Plus Maze ◽  
Folk Medicine ◽  
Anxiolytic Effect ◽  
Transdermal Administration ◽  
Icr Mice ◽  
Elevated Plus Maze Test ◽  
Plus Maze

Tobacco (Nicotiana tabacum Linn.) is a famous traditional herb used in folk medicine. The essential oils of tobacco have been demonstrated in modern studies to possess antioxidant, anti-inflammatory, and neuroprotective properties, while its anxiolytic effect has not been reported. The purpose of this study was to evaluate the anxiolytic effect of Yunnan tobacco essential oil (YTO) and Zimbabwe tobacco essential oil (ZTO) on mice. The constituents of YTO and ZTO were analyzed by GC/MS. The anxiolytic effect of YTO and ZTO (0.1%, 1%, and 10%, v/v) on male ICR mice was evaluated in the light–dark box test (LDB) and the elevated plus maze test (EPM) test via inhalation and transdermal administration. After the behavioral tests, salivary corticosterone levels in mice were measured. The behavioral analysis showed that the administration of both YTO and ZTO elevated the time that the mice spent in the light chamber in the LDB test compared to the untreated control. In the EPM test, YTO and ZTO increased the time spent in open arms and the number of entries into the open arms. In addition, both YTO and ZTO significantly decreased salivary corticosterone levels in mice (p ≤ 0.001). In summary, our results demonstrated that inhalation and transdermal administration of both YTO and ZTO showed anxiolytic effect on male ICR mice.

scholarly journals Adaptogenic activity of Cinnamomum camphora, Eucalyptus globulus, Lavandula stœchas and Rosmarinus officinalis essential oil used in North-African folk medicine

2021 ◽  
Vol 67 (2) ◽  
pp. 83-88
Author(s):  
Mahieddine Boumendjel ◽  
Abdennour Boucheker ◽  
Sandra Feknous ◽  
Faiza Taibi ◽  
Naouel Rekioua ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Emotional Disorders ◽  
Eucalyptus Globulus ◽  
Elevated Plus Maze ◽  
Rosmarinus Officinalis ◽  
Cinnamomum Camphora ◽  
Lavandula Stoechas ◽  
Plus Maze ◽  
Adaptogenic Activity

Depressive anxiety is one of the most emotional disorders in our industrial societies. Many treatments of phobias exist and are based on plant extracts therapies, which play an important role in the amelioration of the behavior. Our study aimed to evaluate the adaptogenic activity of different essential oils provided from local plants: Cinnamomum camphora (Camphora), Eucalyptus globulus (Blue gum), Lavandula stœchas (Topped lavender) and Rosmarinus officinalis (Rosemary) on Wistar rats. The adaptogenic activity was evaluated on the elevated plus-maze. The efficacy of the extract (200 mL/kg) was compared with the standard anxiolytic drug Diazepam® 1 mg. Animals administered by the essential oil of Lavandula stœchas, Cinnamomum camphora, Rosmarinus officinalis and Eucalyptus globulus showed a behavior similar to those treated with Diazepam®. For groups treated with the following essential oils: Rosmarinus officinalis, Lavandula stoechas and Cinnamomum camphora at a dose of 200 mL/kg, we notice an increase in the time spent on the open arms of the elevated plus-maze and a decrease in time spent on the closed arms of the elevated plus-maze, especially for Rosmarinus officinalis, which explains the anxiolytic effect of these plants. We also notice a decrease in the number of entries in closed arms, open arms and the number of passing to the central square. The increase in the number of entries to open arms with Eucalyptus globulus essential oil shows a reduction in anxiety behavior in rodents and this shows that these plants have an inhibitory effect.

scholarly journals Effects of Simultaneous Reinforcement of Endocannabinoid and Cholinergic Systems on Anxiety-Like Behavior in Mice

2021 ◽  
Author(s):  
Siamak Shahidi ◽  
Asghar Dindar ◽  
Alireza Komaki ◽  
Reihaneh Sadeghian
Keyword(s):  
Elevated Plus Maze ◽  
Enzyme Inhibitor ◽  
Endocannabinoid System ◽  
Anxiolytic Effect ◽  
Control Group ◽  
Concurrent Administration ◽  
Elevated Plus Maze Test ◽  
Cholinergic Systems ◽  
Plus Maze ◽  
High Doses

Abstract ObjectiveAnxiety behavior is regulated by different neurotransmitter systems. There has been no direct relationship between endocannabinoid and cholinergic systems on anxiety in previous studies. This study investigated the effects of each of these systems separately and simultaneously using Donepezil (Cholinesterase inhibitor) and URB-597 (endocannabinoid degrading enzyme inhibitor) on anxiety-like behavior. MethodEighty-eight male mice were divided into eleven groups (n=8) including control (saline), diazepam (0.3 mg /kg), URB-597 (0.1, 0.3, or 1 mg /kg), donepezil (0.5, 1 or 2 mg/kg) and the combination of the two drugs at low, medium and high doses. All treatments were injected intraperitoneally 30 minutes before the elevated plus maze test. ResultsSeparate administration of URB597, donepezil or diazepam increased the number and time spent of open arms compared to the control group. Concurrent administration of URB and donepezil at low, medium and high doses did not change the number of open arms entries compared to the control group, but they reduced the number of entries to the closed arms. ConclusionsThese results suggest that strengthening any cholinergic or endocannabinoid system has anxiolytic effect similar to diazepam. However, the interaction of these two systems has fewer anxiolytic effects compared to the effects of each alone. It seems that these drugs alone may represent a strategy for the treatment of anxiety disorders.

Effects of single and combined gabapentin use in elevated plus maze and forced swimming tests

2014 ◽  
Vol 26 (5) ◽  
pp. 307-314 ◽  
Author(s):  
Fatma Sultan Kilic ◽  
Sule Ismailoglu ◽  
Bilgin Kaygisiz ◽  
Setenay Oner
Keyword(s):  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Experimental Models ◽  
Forced Swimming ◽  
Anxiety And Depression ◽  
Antidepressant Effect ◽  
Control Group ◽  
Psychiatric Illnesses ◽  
Plus Maze ◽  
Antidepressant Effects

BackgroundGabapentin, a third-generation antiepileptic drug, is a structural analogue of γ-aminobutyric acid, which is an important mediator of central nervous system. There is clinical data indicating its effectiveness in the treatment of psychiatric illnesses such as bipolar disorder and anxiety disorders.ObjectivesWe aimed to investigate the antidepressant and anxiolytic-like effects and mechanisms of gabapentin in rats.Material and MethodsFemale Spraque–Dawley rats weighing 250±20 g were used. A total of 13 groups were formed, each containing 8 rats: gabapentin (5, 10, 20, 40 mg/kg), amitriptyline (10 mg/kg), sertraline (5 mg/kg), diazepam (5 mg/kg), ketamine (10 mg/kg), gabapentin 20 mg/kg was also combined with amitriptyline (10 mg/kg), sertraline (5 mg/kg), diazepam (5 mg/kg) and ketamine (10 mg/kg). All the drugs were used intraperitoneally as single dose. Saline was administered to the control group. Elevated plus maze and forced swimming tests were used as experimental models of anxiety and depression, respectively.ResultsIt was observed that gabapentin showed an anxiolytic-like and antidepressant-like effect in all doses in rats. Its antidepressant effect was found to be the same as the antidepressant effects of amitriptyline and sertraline. There was no change in the antidepressant effect when gabapentin was combined with amitriptyline and ketamine, but there was an increase when combined with sertraline and diazepam. Gabapentin and amitriptyline showed similar anxiolytic effect, whereas ketamine and diazepam had more potent anxiolytic effect compared with them.ConclusionsThese data suggest that gabapentin may possess antidepressant- and anxiolytic-like effects.

scholarly journals Absence of sibutramine effect on spontaneous anxiety in rats

2010 ◽  
Vol 54 (4) ◽  
pp. 375-380 ◽  
Author(s):  
Silvana S. Frassetto ◽  
Isis O. Alves ◽  
Marislane M. Santos ◽  
Ana E. S. Schmidt ◽  
Janaína J. Lopes ◽  
...  
Keyword(s):  
Chronic Treatment ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Chronic Administration ◽  
Positive Control ◽  
Maximum Effect ◽  
Plus Maze ◽  
The Central Nervous System ◽  
Treatment Of Obesity ◽  
Acute And Chronic Treatments

INTRODUSTION: Sibutramine has been described as a drug recommended for treatment of obesity, since it has the ability to inhibit the reuptake of serotonin and noradrenaline in the central nervous system, thereby increasing energy expenditure. OBJECTIVE: Investigate the anxiogenic and anxiolytic effects of acute and chronic treatment with sibutramine in rats submitted to the task of the elevated plus-maze. METHODS: Diazepam was used as a positive control for the anxiolytic effect, and the task of the elevated plus-maze showed sensitivity to detect the effect. In the chronic treatment, sibutramine was ingested for a period of two months. RESULTS: The acute and chronic treatments at the studied dose, which is described to produce a maximum effect of anti-obesity in rats, did not interfere with anxiety. CONCLUSIONS: The acute and chronic administration of sibutramine is not related to anxiolytic or anxiogenic effects.

scholarly journals Role of 5-HT1A Receptor in the Anxiolytic-Relaxant Effects of Bergamot Essential Oil in Rodent

2020 ◽  
Vol 21 (7) ◽  
pp. 2597 ◽  
Author(s):  
Laura Rombolà ◽  
Damiana Scuteri ◽  
Chizuko Watanabe ◽  
Shinobu Sakurada ◽  
Kengo Hamamura ◽  
...  
Keyword(s):  
Essential Oil ◽  
Elevated Plus Maze ◽  
Relaxant Effect ◽  
Serotonergic Receptors ◽  
Selective Agonist ◽  
Plus Maze ◽  
Way 100635 ◽  
Pharmacological Data ◽  
Citrus Bergamia

The essential oil obtained by the fresh fruit of Citrus bergamia Risso et Poiteau is used worldwide in aromatherapy to reduce pain, facilitate sleep induction, and/or minimize the effects of stress-induced anxiety. Preclinical pharmacological data demonstrate that bergamot essential oil (BEO) modulates specific neurotransmissions and shows an anxiolytic-relaxant effect not superimposable to that of the benzodiazepine diazepam, suggesting that neurotransmissions, other than GABAergic, could be involved. Several studies on essential oils indicate a role for serotonergic (5-HT) neurotransmission in anxiety. Interestingly, among serotonergic receptors, the 5-HT1A subtype seems to play a key role in the control of anxiety. Here, we report that modulation of the 5-HT1A receptor by selective agonist ((±)8-OH-DPAT) or antagonist (WAY-100635) may influence some of the anxiolytic-relaxant effects of BEO in Open Field and Elevated Plus Maze tests.

scholarly journals Anxiolytic effect of clonazepam in female rats: Grooming microstructure and elevated plus maze tests

2012 ◽  
Vol 684 (1-3) ◽  
pp. 95-101 ◽  
Author(s):  
Maurício S. Nin ◽  
Natividade S. Couto-Pereira ◽  
Marilise F. Souza ◽  
Lucas A. Azeredo ◽  
Marcelo K. Ferri ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Female Rats ◽  
Plus Maze

scholarly journals Effects of the Essential Oil from Leaves of Alpinia zerumbet on Behavioral Alterations in Mice

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Shio Murakami ◽  
Mariko Matsuura ◽  
Tadaaki Satou ◽  
Shinichiro Hayashi ◽  
Kazuo Koike
Keyword(s):  
Essential Oil ◽  
Mass Spectra ◽  
Elevated Plus Maze ◽  
Neuropsychiatric Symptoms ◽  
Retention Indices ◽  
Reproductive Hormone ◽  
Behavioral Alterations ◽  
Maze Task ◽  
Plus Maze ◽  
Alpinia Zerumbet

In phytotherapy, the essential oil from the leaves of Alpinia zerumbet ( Alpinia speciosa K. Schum.) (EOAZ) is used for neuropsychiatric symptoms, such as depression, stress and anxiety, and chronic problems that are associated with reproductive hormone imbalances in women. The chemical composition of EOAZ was analyzed by GC/MS, and the EOAZ properties inducing behavioral alterations in mice were examined by behavioral observations (BO) and an elevated plus-maze task (EPM), widely used as a method for assessing anxiolytic-like behaviors. Five major compounds, p-cymene (28.0 ± 5.0%), 1,8-cineole (17.9 ± 4.2%), terpinen-4-ol (11.9 ± 6.3%), limonene (6.3 ± 2.2%), and camphor (5.2 ± 2.1%) were identified by retention indices, mass spectra and comparison with standards. Inhalational administration of EOAZ (8.7 ppm) induced unique jumping behaviors in mice. To further investigate the behavioral regulatory mechanisms of EOAZ, we administered an intraperitoneal injection of either 10 mg/kg 5-HTP or 10 mg/kg fluoxetine prior to the EOAZ inhalations. By 5-HTP or fluoxetine pretreatments, the jumping frequencies were significantly decreased. In EPM, EOAZ (0.087 and 8.7 ppm) obviously showed the anxiolytic-like activity in mice.

scholarly journals Effect on Emotional Behavior and Stress by Inhalation of the Essential oil from Chamaecyparis obtusa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Hikaru Kasuya ◽  
Erika Hata ◽  
Tadaaki Satou ◽  
Masaki Yoshikawa ◽  
Shinichiro Hayashi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Elevated Plus Maze ◽  
Growth Factor Receptor ◽  
Glass Container ◽  
Chamaecyparis Obtusa ◽  
Emotional Behavior ◽  
Antibacterial And Antifungal Activity ◽  
Plus Maze ◽  
Antibacterial And Antifungal ◽  
The Brain

Various effects have been reported in the literature for the essential oil from Chamaecyparis obtusa (EOCO), such as antibacterial and antifungal activity. In this study, we examined the effect of EOCO on emotional behavior and stress-induced biomarkers. Male ICR mice, aged 5 weeks at the start of each experiment, were individually housed in cages for 1 week. After placing each mouse in a glass container and exposing it to EOCO for 90 min, we then investigated the influence on emotional behavior using the elevated-plus maze (EPM) test, which is one of the evaluation methods for anxiolytic-like behavior. Significant anxiolytic-like effects were observed for the 7.0 mg/L air EOCO ( P<0.05). After the EPM test, mice were dissected and changes in the stress-induced biomarkers within the brain were investigated by examining the amounts of fast nerve growth factor receptor (NGFR) and activity regulated cytoskeletal-associated protein (Arc) gene expression, and brain-derived neurotrophic factor (BDNF) and galactokinase 1 (GLK1) protein expression. Significant increases were observed in the amount of NGFR after inhalation of 7.0 mg/L air EOCO ( P<0.05). These results indicate that EOCO has both anxiolytic-like and stress mitigation effects.

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