scholarly journals Canthin-6-one Alkaloids and a Tirucallanoid from Eurycoma longifolia and Their Cytotoxic Activity against a Human HT-1080 Fibrosarcoma Cell Line

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Katsunori Miyake ◽  
Yasuhiro Tezuka ◽  
Suresh Awale ◽  
Feng Li ◽  
Shigetoshi Kadota
Keyword(s):  
Cytotoxic Activity ◽  
Natural Product ◽  
Cell Line ◽  
Positive Control ◽  
Mass Spectrometric ◽  
Fibrosarcoma Cell ◽  
Eurycoma Longifolia ◽  
Phytochemical Investigation ◽  
Eurycoma Longifolia Jack ◽  
First Time

Phytochemical investigation of the stems of Eurycoma longifolia Jack led to the isolation of two new canthin-6-one alkaloids, 4,9-dimethoxycanthin-6-one (1) and 10-hydroxy-11-methoxycanthin-6-one (2), and a new tirucallane-type triterpenoid, 23,24,25-trihydroxytirucall-7-en-3,6-dione (3), along with 37 known compounds. Among these, an oxasqualenoid (4) was isolated as a natural product for the first time. The structures of the isolates were elucidated by spectroscopic and mass spectrometric means. All the isolates were evaluated for their cytotoxic activity against a HT-1080 human fibrosarcoma cell line. Among them, 9,10-dimethoxycanthin-6-one (14, IC50 = 5.0 μM), 10-hydroxy-9-methoxycanthin-6-one (15, IC50 = 7.2 μM), dihydroniloticin (18, IC50 = 8.2 μM), and 14-deacetyleurylene (34, IC50 = 3.2 μM) displayed stronger activity than the positive control 5-FU (IC50 = 9.2 μM).

scholarly journals Cytotoxic Activity of Eurycoma Longifolia Jack Root Extract Against Nasopharyngeal Carcinoma Cell Line

2021 ◽  
Vol 13 (4) ◽  
pp. 1014-1018
Author(s):  
Nur Haseena Kajahmohideen ◽  
Siti Nur Syafiqah Razi ◽  
Ghasak Ghazi Faisal ◽  
Abdelkader Elbadawy Ashour ◽  
Anisa Kusumawardani ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Root Extract ◽  
Eurycoma Longifolia ◽  
Nasopharyngeal Carcinoma Cell ◽  
Eurycoma Longifolia Jack

Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

2020 ◽  
Vol 17 (3) ◽  
pp. 206-210
Author(s):  
Ty Viet Pham ◽  
Thang Quoc Le ◽  
Anh Tuan Le ◽  
Hung Quoc Vo ◽  
Duc Viet Ho
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Structural Determination ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
Phytochemical Constituents ◽  
First Time ◽  
Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

scholarly journals Flavonoids from Milletia leucantha and Their Cytotoxicity

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Uraiwan Sriphana ◽  
Chavi Yenjai ◽  
Siriporn Tungnoi ◽  
Jongjai Srirapa ◽  
Auemporn Junsongduang
Keyword(s):  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Line ◽  
Vero Cells ◽  
2D Nmr ◽  
Nmr Techniques ◽  
Vero Cell Lines ◽  
First Time ◽  
Flavonol Derivatives ◽  
Mcf 7

Two known chalcones (1–2), five known flavonol derivatives (3 and 5–8) and one flavone (4) were isolated for the first time from fruits of Millettia leucantha Kurz. In addition, 5 was found for the first time from this genus. Their structures were elucidated on the basis of IR, MS, 1D and 2D NMR techniques. Chalcone 1 exhibited moderate cytotoxicity against the MCF-7 cell line with an IC50 value of 50.93 μM, whereas this compound showed inactive cytotoxicity against Vero cells. Compound 2 exhibited cytotoxicity against the KB, NCI-H187 and Vero cell lines with IC50 values of 63.64, 114.44 and 28.44 μM, respectively. Compound 8 only showed cytotoxic activity against the KB cell line with an IC50 value of 110.23 μM.

scholarly journals New Verrucosidin Derivatives from the Marine-Derived Fungus Penicillium sp. XL-01

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Yan Xu ◽  
Miao Zhang ◽  
Qing-Ai Liu ◽  
Lian-Dong Hu ◽  
Wan Li ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Absolute Configuration ◽  
Quantum Chemical Calculation ◽  
Quantum Chemical ◽  
Bohai Sea ◽  
Positive Control ◽  
Spectroscopic Techniques ◽  
Chemical Calculation ◽  
Penicillium Sp

Two new verrucosidin derivatives, named nordeoxyverrucosidin (1) and norverrucosidinol acetate (2), along with three known analogues (3–5) were isolated from the marine-derived fungus Penicillium sp. XL-01 collected from the Bohai Sea. Their structures and relative configurations were elucidated by comparing with literature data and a combination of spectroscopic techniques. The absolute configuration of 1 was assigned by quantum chemical calculation of the electronic circular dichroism (ECD) spectrum. Compounds 1 and 3 displayed promising cytotoxic activity against the MGC-803 cell line, with IC50 values of 0.96 and 1.14 μM, respectively, more effective than the positive control (cisplatin).

scholarly journals Bioactive Components of Fissistigma cupreonitens

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
I-Hsiao Chen ◽  
Ming-Yi Yang ◽  
Shin-Hun Juang ◽  
Chia-Lin Lee ◽  
Tran-Dinh Thang ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cancer ◽  
Spectroscopic Methods ◽  
Human Cancer Cell ◽  
Bioactive Components ◽  
Standard Drug ◽  
Human Cancer Cell Lines ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Phytochemical investigation of Fissistigma cupreonitens (Annonaceae) led to the isolation of 34 compounds. The chemical structures of all compounds were determined by spectroscopic methods. Among the isolates, compounds 20–27 and 31–34 were reported from this genus for the first time. From the results of the cytotoxicity assay against three human cancer cell lines (NCI-H226, NPC-TW01, and Jurkat E6–1), oxoaporphine compounds oxoxylopine (1), oxocrebanine (3), kuafumine (4) and lysicamine (5), and the flavonoid adunctin E (26) displayed significant cytotoxicity against NCI-H226 cell line, with IC50 values of 8.45, 8.10, 8.54, 12.83 and 12.00 μM, respectively, in comparison with the standard drug, cisplatin with IC50 of 13.37 μM.

scholarly journals Chemical Constituents of the Roots and Rhizomes of Saposhnikovia divaricata and their Cytotoxic Activity

2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Akihito Yokosuka ◽  
Satoru Tatsuno ◽  
Takuma Komine ◽  
Yoshihiro Mimaki
Keyword(s):  
Cytotoxic Activity ◽  
Apoptotic Cell ◽  
Chemical Constituents ◽  
Leukemia Cells ◽  
Caspase 9 ◽  
Meoh Extract ◽  
Apoptotic Pathway ◽  
Phytochemical Investigation ◽  
Saposhnikovia Divaricata ◽  
First Time

Phytochemical investigation of the MeOH extract of the roots and rhizomes of Saposhnikovia divaricata (Umbelliferae) resulted in the isolation of six chromons (1-6) and five polyacetylene derivatives (7-11). Compounds 9 and 11 were isolated from S. divaricate for the first time. The chromon derivatives (1-6) were evaluated for their cytotoxic activity against HL-60 human promyelocytic leukemia cells. Compound 1 (3′- O-angeloylhamaudol) showed the most potent cytotoxic activity with an IC50 value of 4.41 μM and was found to induce apoptotic cell death in HL-60 cells. The loss of mitochondrial membrane potential, release of cytochrome c into the cytoplasm, and activation of caspase-9 in the 1-treated HL-60 cells suggests that 1 induces apoptosis through the mitochondrial-dependent apoptotic pathway.

scholarly journals New Secodaphnane-Type Alkaloids with Cytotoxic Activities from Daphniphyllum angustifolium Hutch

2021 ◽  
Author(s):  
Qing-Yun Lu ◽  
Jia-Hui Zhang ◽  
Ying-Yao Li ◽  
Xue-Xue Pu ◽  
Cui-Shan Zhang ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Natural Product ◽  
Cell Line ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Hela Cell Line

AbstractOne new Daphniphyllum alkaloid, daphnioldhanol A (1), together with three known ones, were isolated from the stem part of Daphniphyllum angustifolium Hutch. Their structures were elucidated by spectroscopic methods and comparing with the literature data. Compound 2 is a new natural product, but known by synthesis as a racemate. Compound 1 exhibited week cytotoxic activity against Hela cell line with IC50 of 31.9 μM. Graphic Abstract

scholarly journals Cytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Adisty Ridha Damasuri ◽  
Eti Nurwening Sholikhah ◽  
Mustofa
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Colorimetric Assay ◽  
Cancer Cell Line ◽  
Positive Control ◽  
Probit Analysis ◽  
Hela Cell Line ◽  
Chalcone Derivatives ◽  
Cell Line Hela

In our previous study, some amino chalcone derivatives have been synthesized and evaluated their cytotoxicity against breast cancer cell line T47D. Among 11 amino chalcone derivatives, ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) exhibited the most active compound.This study aimed to investigate cytotoxic activity of the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) against cervical cell line (HeLa). The cytotoxic activitywas determined using the MTT colorimetric assay. Cisplatin was used as positive control. From this MTT method, inhibitory concentration 50% (IC50) values were determined by probit analysis based on the relationship between log concentrations versus the percentage of cells growth inhibition.The results showed that the IC50 of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) and cisplatinwere 22.75 ± 19.13 μg/mL and 14.96±1.08 μg/mL, respectively. In conclusion, the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) has moderate cytotoxic activity against HeLa cell line based on National Cancer Institute (NCI) criteria.

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