scholarly journals Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Eschscholzia californica (Papaveraceae)

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Lucie Cahlíková ◽  
Kateřina Macáková ◽  
Jiří Kuneš ◽  
Milan Kurfürst ◽  
Lubomír Opletal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Human Blood ◽  
Inhibitory Activity ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Isoquinoline Alkaloids ◽  
Eschscholzia Californica ◽  
Aerial Parts ◽  
Inhibitory Compounds

The roots and aerial parts of Eschscholzia californica Cham. (Papaveraceae) were extracted with ethanol. Repeated column chromatography, preparative TLC, and crystallization led to the isolation of fourteen isoquinoline alkaloids, the structures of which were determined on the basis of spectroscopic techniques and by comparison with literature values. One of the compounds isolated, 1-(3-hydroxy-4-methoxybenzyl)-2-methyl-6,7-methylenedioxy-1,2,3,4-tetrahydroisoquinoline (14), has not previously been isolated from a natural source. All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibition activity. None of the compounds isolated significantly inhibited both HuAChE and HuBuChE, but the two benzylisoquinoline alkaloids, reticuline 9 and 14, showed promising inhibitory activity against HuBuChE.

scholarly journals Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium Majus (Papaveraceae)

2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Lucie Cahlíková ◽  
Lubomír Opletal ◽  
Milan Kurfürst ◽  
Kateřina Macáková ◽  
Andrea Kulhánková ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Inhibitory Activity ◽  
Dependent Manner ◽  
Isoquinoline Alkaloids ◽  
Chelidonium Majus ◽  
Aerial Parts ◽  
Naturally Occurring ◽  
Inhibitory Compounds ◽  
Dose Dependent

The roots and aerial parts of Chelidonium majus L. were extracted with EtOH and fractionated using CHCl3 and EtOH. Repeated column chromatography, preparative TLC and crystallization led to the isolation of five isoquinoline alkaloids, stylopine (3), chelidonine (4), homochelidonine (5), protopine (6), and allocryptopine (7), along with two isolation artifacts 6-ethoxydihydrosanguinarine (1) and 6-ethoxydihydrochelerythrine (2). All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. The isolation artifacts exhibited the highest activity against HuAChE and HuBuChE with IC50 values of 0.83 ± 0.04 μM and 4.20 ± 0.19 μM for 6-ethoxydihydrochelerythrine and 3.25 ± 0.24 μM and 4.51 ± 0.31 μM for 6-ethoxydihydrosanguinarine. The most active of the naturally-occurring alkaloids was chelidonine, which inhibited both HuAChE and HuBuChE in a dose-dependent manner with IC50 values of 26.8 ± 1.2 μM and 31.9 ± 1.4 μM, respectively.

scholarly journals Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Corydalis Cava (Fumariaceae)

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Jakub Chlebek ◽  
Kateřina Macáková ◽  
Lucie Cahlíková ◽  
Milan Kurfürst ◽  
Jiří Kuneš ◽  
...  
Keyword(s):  
Human Blood ◽  
Inhibitory Activity ◽  
Potent Inhibitor ◽  
The Other ◽  
Spectroscopic Techniques ◽  
Dependent Manner ◽  
Isoquinoline Alkaloids ◽  
Chemical Structures ◽  
Inhibitory Compounds ◽  
Dose Dependent

Tubers of Corydalis cava were extracted with ethanol and fractionated using n-hexane, chloroform and ethanol. Repeated column chromatography, preparative TLC and crystallization led to the isolation of fifteen isoquinoline alkaloids. The chemical structures of the isolated compounds were determined on the basis of spectroscopic techniques and by comparison with literature data. All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. (+)-Canadaline inhibited acetylcholinesterase as well as butyrylcholinesterase in a dose-dependent manner with IC50 values of 20.1 ± 1.1 μM and 85.2 ± 3.2 μM, respectively. (+)-Canadine, with an IC50 value of 12.4 ± 0.9 μM, was the most potent inhibitor of acetylcholinesterase, whilst (±)-corycavidine and (+)-bulbocapnine were effective inhibitors of butyrylcholinesterase with IC50 values of 46.2 ± 2.4 uM and 67.0 ± 2.1 μM, respectively. The other isolated alkaloids were considered inactive (IC50 > 100 μM).

scholarly journals Corylucinine, a new Alkaloid from Corydalis cava (Fumariaceae), and its Cholinesterase Activity

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Zdeněk Novák ◽  
Jakub Chlebek ◽  
Lubomír Opletal ◽  
Pavel Jiroš ◽  
Kateřina Macáková ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Inhibitory Activity ◽  
Cholinesterase Activity ◽  
Optical Rotation ◽  
2D Nmr ◽  
Esi Ms

A new benzyldihydroisoquinoline alkaloid (1) was isolated from the tubers of Corydalis cava and named corylucinine. Additionally, 8-trichloromethyl-7,8-dihydropalmatine (2), an isolation artifact of tetrahydropalmatine, was obtained. The structures were established by spectroscopic (including 2D NMR and optical rotation) and HR-ESI-MS methods. Both compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. In comparison with the used standards, both compounds showed only moderate inhibitory activity against HuAChE (IC50, HuAChE = 127.6 ± 5.2 μM for 1, and IC50, HuAChE = 82.9 ± 3.9 μM for 2) and none against HuBuChE.

scholarly journals Antioxidant and α-Glucosidase Inhibitory Constituents from Hornstedtia Species of Malaysia

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Siti Ernieyanti Hashim ◽  
Hasnah Mohd Sirat ◽  
Khong Heng Yen ◽  
Intan Safinar Ismail ◽  
Siti Nurulhuda Matsuki
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Dpph Assay ◽  
Glucosidase Inhibitors ◽  
Antioxidant Agent ◽  
First Time

Seven compounds were isolated from the n-hexane and chloroform extracts of the flowers and leaves of four Hornstedtia species and their structures were identified using spectroscopic techniques as 3,7,4′-trimethylkaempferol (1), 3,7-dimethylkaempferol (2), 7,4′-dimethylkaempferol (3), 3,5-dimethylkaempferol (4), 3-methylkaempferol (5), stigmast-4-en-3-one (6), and 6-hydroxy-stigmast-4-en-3-one (7). Compounds 1 to 7 were isolated from these species for the first time. They were assayed for free radical scavenging and α-glucosidase inhibition activities. The DPPH assay showed that 3-methylkaempferol (5) was the most potent antioxidant agent with an IC50 value 78.6 μM, followed by 7,4′-dimethylkaempferol (3) (IC50 = 86.1 μM). For α-glucosidase inhibition activity, 3-methylkaempferol (5) exhibited significant inhibitory activity with an IC50 value 21.0 μM. The present study revealed that Hornstedtia species have potential activities as antioxidant and α-glucosidase inhibitors.

Some Chemical Constituents Of Terminalia Glaucescens And Their Enzymes Inhibition Activity

2005 ◽  
Vol 60 (3) ◽  
pp. 347-350 ◽  
Author(s):  
Atta-ur Rahman ◽  
Seema Zareen ◽  
M. Iqbal Choudhary ◽  
M. Nadeem Akhtar ◽  
Atta-ur Rahman ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Chemical Constituents ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Enzymes Inhibition

A new triterpenoid, glaucinoic acid (2α, 3β, 19α, 24-tetrahydroxyolean-12-en-30-oic acid) (1) along with several known compounds, arjunic acid (2), arjungenin (3), sericoside (4), and friedelin (5) were isolated from the stem barks of Terminalia glaucescens. These compounds showed β - glucuronidase inhibitory activity. The structures were identified on the basis of spectroscopic techniques

scholarly journals Analysis of Amaryllidaceae Alkaloids from Chlidanthus Fragrans by GC-MS and their Cholinesterase Activity

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600
Author(s):  
Lucie Cahlíková ◽  
Kateřina Macáková ◽  
Stanislav Zavadil ◽  
Pavel Jiroš ◽  
Lubomír Opletal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Inhibitory Activity ◽  
Cholinesterase Activity ◽  
Capillary Gc ◽  
Amaryllidaceae Alkaloids ◽  
First Time

The underivatized alkaloid mixture extracted from the bulbs of Chlidanthus fragrans Herb. was investigated by capillary GC/MS for the first time. Fifteen known Amaryllidaceae alkaloids of five structure types were identified. The main alkaloids were tazzetine (9, tazettine-type), chlidanthine (2, galanthamine-type), belladine (8, belladine-type) and lycorine (12, lycorine-type). The alkaloid extract from the bulbs showed promising human blood acetylcholinesterase (IC50 = 20.1 ± 2.9 μg/mL) and human plasma butyrylcholinesterase (IC50 = 136.8 ± 6.9 μg/mL) inhibitory activity.

Plasma Thromboplastin Component (Christmas Factor, Factor IX) Levels in Stored Human Blood and Plasma

1960 ◽  
Vol 04 (03) ◽  
pp. 376-388 ◽  
Author(s):  
J Dieter Geratz ◽  
John B. Graham
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Whole Blood ◽  
Room Temperature ◽  
Close Agreement ◽  
Factor Ix ◽  
Deficient Patient ◽  
Glass Tubes ◽  
Disodium Hydrogen Citrate ◽  
Bank Blood

Summary1. PTC activity was assayed in 26 units of human plasma prepared from whole blood stored for 3 weeks at 4° C. The plasma had been frozen and stored at — 20° C for additional periods ranging from a few days to 4 months. High PTC activity was still present in the plasma at the end of this period, the activity averaging 95% of normal.2. The PTC activity of 19 samples of “reclaimed“ plasma stored for an additional 6 months at — 20° C decreased by an average of 23%. This decrease was statistically significant.3. Liquid plasma kept at room temperature for 5½—7½ months contained no PTC activity.4. Lyophilized plasma stored at room temperature for 6—8 years contained an average of 30% PTC activity. Lyophilized plasma stored at — 20° C for 4 years contained 68% PTC activity.5. ACD and disodium hydrogen citrate anticoagulant solutions served equally well in preserving PTC activity in whole blood stored in glass tubes over a period of 3 weeks at 4° C.6. “Reclaimed“ plasma from outdated bank blood provided effective hemostasis in two operations for the removal of 20 teeth from a severely PTC-deficient patient.7. The high PTC activity of “reclaimed“ plasma was confirmed by the close agreement between the PTC level expected in a PTC deficient patient after transfusion of such plasma and that observed.

scholarly journals Modulation of Oxidative Stress and Hemostasis by Flavonoids from Lentil Aerial Parts

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 497
Author(s):  
Jerzy Żuchowski ◽  
Agata Rolnik ◽  
Weronika Adach ◽  
Anna Stochmal ◽  
Beata Olas
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Human Plasma ◽  
Crude Extract ◽  
Lens Culinaris ◽  
Bioactive Substances ◽  
Phenolic Fraction ◽  
Aerial Parts ◽  
Scientific Attention

While specific metabolites of lentil (Lens culinaris L.) seeds and their biological activity have been well described, other organs of this plant have attracted little scientific attention. In recent years, green parts of lentils have been shown to contain diverse acylated flavonoids. This work presents the results of the research on the effect of the crude extract, the phenolic fraction, and seven flavonoids obtained from aerial parts of lentils on oxidative damage induced by H2O2/Fe to lipid and protein constituents of human plasma. Another goal was to determine their effect on hemostasis parameters of human plasma in vitro. Most of the purified lentil flavonoids had antioxidant and anticoagulant properties. The crude extract and the phenolic fraction of lentil aerial parts showed antioxidant activity, only at the highest tested concentration (50 μg/mL). Our results indicate that aerial parts of lentils may be recommended as a source of bioactive substances.

Molecular Imprinted Graphene Oxide Nanocomposite for Optical Sensing of Nicotine in Human Blood Plasma

2020 ◽  
Vol 42 (6) ◽  
pp. 856-856
Author(s):  
Sehrish Qazi Sehrish Qazi ◽  
Huma Shaikh Huma Shaikh ◽  
Ayaz Ali Memon Ayaz Ali Memon ◽  
and Shahabuddin Memon and Shahabuddin Memon
Keyword(s):  
Graphene Oxide ◽  
Human Plasma ◽  
Human Blood ◽  
Optical Sensor ◽  
Single Layer ◽  
Human Blood Plasma ◽  
Buffer Concentration ◽  
Imprinted Polymer ◽  
Ft Ir

Among all psychotropic alkaloids, nicotine is more addictive, carcinogenic and capable of causing many health problems. This work is based on the development of highly robust, cheap, reliable, selective and sensitive nicotine imprinted graphene oxide nanocomposite (ImpGO nanocomposite) based optical sensor for determination of nicotine in human plasma. The ImpGO nanocomposite has been thoroughly characterized using different techniques i.e. FT-IR, SEM, TEM, Raman, etc. These characterizations revealed that ImpGO nanocomposite is comprised of single layer of graphene oxide successfully modified with imprinted polymer. The synthesized material was utilized to selectively determine nicotine using UV-vis spectrophotometer in BR buffer of 0.1 M at pH 3 and diluted human plasma. The effect of parameters such as buffer concentration, pH, amount of nanocomposite, etc on determination of nicotine using ImpGO nanocomposite were studied thoroughly. Thus, a sensitive optical method was developed for determination of nicotine in human plasma with linear range of 22-370 pM along with LOD and LOQ of 7 pM and 22 pM, respectively. The selectivity of sensor was evaluated using homologues of nicotine such as nicotine amide, caffeine and cotinine. The results obtained from biological samples showed that developed optical sensor is efficient in complex matrices of real sample.

scholarly journals ALPHA AMYLASE AND ALPHA GLUCOSIDASE INHIBITION ACTIVITY OF SELECTED EDIBLE SEAWEEDS FROM SOUTH COAST AREA OF INDIA

2017 ◽  
Vol 9 (6) ◽  
pp. 64 ◽  
Author(s):  
P. Reka ◽  
Thahira Banu A. ◽  
M. Seethalakshmi
Keyword(s):  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Alpha Amylase ◽  
Postprandial Blood Glucose ◽  
Inhibition Activity ◽  
Gracilaria Edulis ◽  
Edible Seaweeds ◽  
Daily Diet ◽  
Postprandial Blood Glucose Level ◽  
Alpha Glucosidase

Objective: The present work was to investigate the alpha amylase and alpha-glucosidase inhibitory activity of the selected edible seaweeds.Methods: The seaweeds namely Acanthophora spicifera, Gracilaria corticata, Gracilaria edulis, Ulva lactuca and Ulva reticulata were selected for this study. Six and eight hours of ethanol and aqueous extract were used for the estimation of alpha amylase using DNS method and alpha-glucosidase inhibition activity.Results: The study reported that the solvent from ethanol and aqueous in eight hours of extraction showed a higher inhibitory activity than six hours of extraction. Maximum of 89.1±0.96 and 79.55±3.08 percent of alpha-amylase and alpha-glucosidase inhibition activity were detected in the eight hours of aqueous extract (0.5 ml) of Ulva reticulata and Gracilaria edulis respectively. All the selected edible seaweeds had significant differences (p<0.05) in alpha amylase and alpha glucosidase inhibition activity between the selected seaweeds with different extracts.Conclusion: It was concluded that all the selected edible seaweeds have the potential to act as a potent inhibitor of the carbohydrate hydrolyzing enzyme. Thus, it was clear from the study that seaweeds incorporated in small amounts in the dishes consumed in the daily diet can bring a control on postprandial blood glucose level.

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