New Bioactive Secondary Metabolites from Bornean Red Alga, Laurencia similis (Ceramiales)

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Takashi Kamada ◽  
Charles Santhanaraju Vairappan
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Red Alga ◽  
Algal Population ◽  
Cytotoxic Effects ◽  
Antibiotic Resistant ◽  
Good Antibacterial Activity ◽  
Bioactive Secondary Metabolites ◽  
Red Algal

A Bornean red algal population of Laurencia simlis Nam et Saito was analyzed for its secondary metabolite composition. Seven compounds were identified: ent -1(10)-aristolen-9β-ol (1), (+)-aristolone (2), axinysone B (3), 9-aristolen-1α-ol (4), 2,3,5,6-tetrabromoindole (5), 1-methyl-2,3,5,6-tetrabromoindole (6), and 1-methyl-2,3,5-tribromoindole (7). Compound 1 was identified as a new optical isomer of 1(10)-aristolen-9β-ol. Compounds 1, 4 and 5 exhibited good antibacterial activity against antibiotic resistant clinical bacteria and cytotoxic effects against selected cancer cell lines.

scholarly journals INFLUENCE OF PHYSICO-CHEMICAL PARAMETERS ON SECONDARY METABOLITE PRODUCTION BY MARINE FUNGI

2017 ◽  
pp. 112-118
Author(s):  
Anuhya G. ◽  
Jyostna V. ◽  
Aswani Kumar Yvv ◽  
Bodaiah B. ◽  
Sudhakar P.
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Inhibitory Concentration ◽  
Marine Fungi ◽  
Mineral Salts ◽  
Potato Dextrose Broth ◽  
Physico Chemical ◽  
Bioactive Secondary Metabolites

Objective: Our study aimed to characterize and optimize the physico-chemical properties which render the high yield of bioactive secondary metabolites from marine fungi and its antibacterial activity against clinical pathogens.Methods: Bioactive secondary metabolites extracted from marine fungi of Nijampatnam mangroves, Guntur district Andhra Pradesh, India. By using different solvent systems, secondary metabolite was screened for antimicrobial activity against clinical pathogen, Optimization of cultural conditions for maximizing the yield of biomass and compared the yield in different broth media and minimum inhibitory concentration of the optimised compound done for both compounds.Results: fungal extracts GAPS-1 and GAPS-2 was affectively extracted in ethyl acetate. Isolated compound showed antibacterial activity highest zone of inhibition against E. coli and least to Pseudomonas. Maximum yield of the compound was achieved in modified potato dextrose broth (PDB) medium containing 20% w/v Potato 2% w/v Dextrose broth containing1% w/v glucose and sucrose,1% w/v beef extract and ammonium chloride and 0.01 % mineral salts(MGSO4 and MNSO4) at 25 °C and 30 °C and pH 7.0 and 8.0 with 144 h of incubation period. The yield was observed maximum in potato dextrose broth compared to czapek dox broth, sabaraud’s broth and nutrient broth and minimum inhibitory concentration of the optimized compound ranges from 250 μg for gaps-1 and 300 μg for gaps-2.Conclusion: Secondary metabolite yield maximum in potato dextrose broth and its potential anti-bacterial activity needs further investigation for pharmaceutical applications.

AIE Active Polyelectrolyte Based Photosensitizers: The Effects of Structure on Antibiotic-Resistant Bacterial Sensing and Killing, and Pollutant Decomposition

2021 ◽  
Author(s):  
Hui Xie ◽  
Wanshan Hu ◽  
Fei Zhang ◽  
Changbo Zhao ◽  
Tingting Peng ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Detection Sensitivity ◽  
Bacteria Detection ◽  
Great Promise ◽  
Antibiotic Resistant ◽  
Dye Decomposition ◽  
Good Antibacterial Activity ◽  
Bacterial Sensing ◽  
Decomposition Efficiency ◽  
Multifunctional Platform

A facile and effective multifunctional platform with high bacteria detection sensitivity, good antibacterial activity, and excellent dye decomposition efficiency holds great promise for wastewater treatment. To explore design rationality and...

scholarly journals AN IMPLICATION OF ACTINOMYCETES ON HUMAN WELL-BEING: A REVIEW

2019 ◽  
pp. 11-18
Author(s):  
ALI MOHAMMED ABDULLAH BAWAZIR ◽  
PALAKSHA ◽  
MANJULA SHANTARAM ◽  
MANJULA SHANTARAM
Keyword(s):  
Antimicrobial Resistance ◽  
Secondary Metabolites ◽  
Human Health ◽  
Antimicrobial Agents ◽  
Well Being ◽  
Pathogenic Microorganisms ◽  
Antibiotic Resistant ◽  
Bioactive Secondary Metabolites ◽  
Resistant Strains ◽  
All Diseases

This review conceptualizes about the actinomycetes and its contribution to human health by playing a key role as bioactive secondary metabolites, such as enzymes, antibiotics and pigments, leading to their diverse applications and use in various industries. These searches have been uncommonly successful, and around 66% of naturally happening antibiotics, including many medically important, have been isolated from actinomycetes. The speedy occurrence of antimicrobial resistance among pathogens has led to a renewed interest to search for novel antimicrobial agents, but these antibiotics are not enough for the treatment of all diseases because there is a berserk requirement for a novel actinomycetes to combat against the antibiotic-resistant strains of pathogenic microorganisms, which are quickly expanding bit by bit. Actinomycetes are the important providers to the pharmaceutical and other industries and are well known for their capacity to produce secondary metabolites many of which are active against pathogenic microorganisms.

scholarly journals New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoran Liu ◽  
Ruiyun Huo ◽  
Yanan Zhai ◽  
Ling Liu
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Mcf7 Cell ◽  
Cytotoxic Effects ◽  
Electronic Circular Dichroism

Three new secondary metabolites pestalothenins A–C (1–3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4–8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1–3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1–8 were also evaluated for antibacterial activity.

scholarly journals Cytotoxicity and Antibacterial Potential of Halogenated Chamigrenes from Malaysian Red Alga, Laurencia majuscula

2019 ◽  
Vol 6 (02) ◽  
pp. e36-e40
Author(s):  
Takashi Kamada ◽  
Chin-Soon Phan ◽  
Tatsufumi Okino ◽  
Charles Santhanaraju Vairappan
Keyword(s):  
Spectroscopic Data ◽  
2D Nmr ◽  
Red Alga ◽  
Antibiotic Resistant ◽  
North Borneo ◽  
Bioactive Secondary Metabolites ◽  
Laurencia Majuscula ◽  
Mcf 7 ◽  
Antibacterial Potential ◽  
New Metabolites

AbstractRed algae of the genus Laurencia have been known to produce a wide array of bioactive secondary metabolites. Here, we report the isolation of two new halogenated chamigrenes, lauremantanones A (1) and B (2), along with seven known compounds, dendroidiol (3), (+)-elatol (4), cartilagineol (5), obtusol (6), (+)-laurencenone B (7), 2-chloro-3-hydroxy-α-chamigren-9-one (8), and puertitol A (9), from a population of Laurencia majuscula (Harvey) Lucas from Mantanani Island (North Borneo). The structures of the two new metabolites were determined based on spectroscopic data (IR, 1D and 2D NMR, and MS). Compounds isolated from this alga exhibited potent cytotoxic (HeLa, MCF-7, P-388) and antibacterial (against antibiotic-resistant clinical bacteria) activities. The major metabolite of this population has significant importance in the geographical distribution of this species globally.

scholarly journals Lysobacter enzymogenes Uses Two Distinct Cell-Cell Signaling Systems for Differential Regulation of Secondary-Metabolite Biosynthesis and Colony Morphology

2013 ◽  
Vol 79 (21) ◽  
pp. 6604-6616 ◽  
Author(s):  
Guoliang Qian ◽  
Yulan Wang ◽  
Yiru Liu ◽  
Feifei Xu ◽  
Ya-Wen He ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cell Signaling ◽  
Secondary Metabolite ◽  
Colony Morphology ◽  
Content Type ◽  
Signaling Systems ◽  
Lysobacter Enzymogenes ◽  
Bioactive Secondary Metabolites ◽  
Secondary Metabolite Biosynthesis ◽  
First Time

ABSTRACTLysobacter enzymogenesis a ubiquitous environmental bacterium that is emerging as a potentially novel biological control agent and a new source of bioactive secondary metabolites, such as the heat-stable antifungal factor (HSAF) and photoprotective polyene pigments. Thus far, the regulatory mechanism(s) for biosynthesis of these bioactive secondary metabolites remains largely unknown inL. enzymogenes. In the present study, the diffusible signal factor (DSF) and diffusible factor (DF)-mediated cell-cell signaling systems were identified for the first time fromL. enzymogenes. The results show that both Rpf/DSF and DF signaling systems played critical roles in modulating HSAF biosynthesis inL. enzymogenes. Rpf/DSF signaling and DF signaling played negative and positive effects in polyene pigment production, respectively, with DF playing a more important role in regulating this phenotype. Interestingly, only Rpf/DSF, but not the DF signaling system, regulated colony morphology ofL. enzymgenes. Both Rpf/DSF and DF signaling systems were involved in the modulation of expression of genes with diverse functions inL. enzymogenes, and their own regulons exhibited only a few loci that were regulated by both systems. These findings unveil for the first time new roles of the Rpf/DSF and DF signaling systems in secondary metabolite biosynthesis ofL. enzymogenes.

scholarly journals Diversity of Myxobacteria—We Only See the Tip of the Iceberg

2018 ◽  
Vol 6 (3) ◽  
pp. 84 ◽  
Author(s):  
Kathrin Mohr
Keyword(s):  
New Species ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Rich Source ◽  
Cytostatic Activity ◽  
Bioactive Metabolites ◽  
Plate Count ◽  
Bacillus Species ◽  
Bioactive Secondary Metabolites ◽  
New Antibiotics

The discovery of new antibiotics is mandatory with regard to the increasing number of resistant pathogens. One approach is the search for new antibiotic producers in nature. Among actinomycetes, Bacillus species, and fungi, myxobacteria have been a rich source for bioactive secondary metabolites for decades. To date, about 600 substances could be described, many of them with antibacterial, antifungal, or cytostatic activity. But, recent cultivation-independent studies on marine, terrestrial, or uncommon habitats unequivocally demonstrate that the number of uncultured myxobacteria is much higher than would be expected from the number of cultivated strains. Although several highly promising myxobacterial taxa have been identified recently, this so-called Great Plate Count Anomaly must be overcome to get broader access to new secondary metabolite producers. In the last years it turned out that especially new species, genera, and families of myxobacteria are promising sources for new bioactive metabolites. Therefore, the cultivation of the hitherto uncultivable ones is our biggest challenge.

scholarly journals Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Fitria Ningsih ◽  
Dhian Chitra Ayu Fitria Sari ◽  
Shuhei Yabe ◽  
Akira Yokota ◽  
Wellyzar Sjamsuridzal
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Polyketide Synthase ◽  
Gene Clusters ◽  
Gellan Gum ◽  
Biosynthetic Gene ◽  
Bacterial Strains ◽  
Biosynthetic Gene Clusters ◽  
Novel Taxa

Abstract. Ningsih F, Sari DCAF, Yabe S, Yokota A, Sjamsuridzal W. 2020. Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella. Biodiversitas 21: 5674-5684. Microbial resistance to available antibiotics has gained increasing attention in recent years and led to the urgent search for active secondary metabolites from novel microbial taxa. This study aimed to assess putative secondary metabolite biosynthetic gene clusters (BGCs) in the genome of a novel thermophilic Actinobacteria type strain Gandjariella thermophila SL3-2-4T and screen for its antibacterial activity. Four other related novel candidate Actinobacteria strains, isolated from forest soil in the Cisolok geothermal area (West Java, Indonesia), were also screened for antibacterial activity in various media solidified with gellan gum. The genome of the SL3-2-4T strain contained 21 antiSMASH-identified secondary metabolite regions harboring BGCs. These BGCs were for polyketide synthase, non-ribosomal peptide synthase, and ribosomally synthesized and post-translationally modified peptide family clusters. Three BGC regions displayed 50-100% similarity with known secondary metabolites. Thirteen and five regions displayed low (4-35%) and no similarity with known BGCs for secondary metabolites, respectively. Strains SL3-2-4T and SL3-2-7 on MM 2 medium solidified with gellan gum at 45 °C for 14 days demonstrated inhibitory activity against all Gram-positive, but not Gram-negative bacteria. Strain SL3-2-10 on ISP 3 gellan gum medium incubated for seven days only active against K. rhizophila NBRC 12078. Strains SL3-2-6 and SL3-2-9 did not exhibit any antibacterial activity against the tested bacterial strains on the three tested media. The results indicated that novel taxa have the potential for the discovery of active secondary metabolites.

scholarly journals Antifouling Activity of Halogenated Compounds Derived from the Red Alga Sphaerococcus coronopifolius: Potential for the Development of Environmentally Friendly Solutions

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 32
Author(s):  
Maxence Quémener ◽  
Stefanos Kikionis ◽  
Marilyne Fauchon ◽  
Yannick Toueix ◽  
Fanny Aulanier ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Green Chemistry ◽  
Red Alga ◽  
Point Of View ◽  
Economic Losses ◽  
Research Strategies ◽  
Antifouling Paints ◽  
Antifouling Activity ◽  
Bioactive Secondary Metabolites ◽  
Adhesion Process

Nowadays, biofouling is responsible for enormous economic losses in the maritime sector, and its treatment with conventional antifouling paints is causing significant problems to the environment. Biomimetism and green chemistry approaches are very promising research strategies for the discovery of new antifouling compounds. This study focused on the red alga Sphaerococcus coronopifolius, which is known as a producer of bioactive secondary metabolites. Fifteen compounds, including bromosphaerol (1), were tested against key marine biofoulers (five marine bacteria and three microalgae) and two enzymes associated with the adhesion process in macroalgae and invertebrates. Each metabolite presented antifouling activity against at least one organism/enzyme. This investigation also revealed that two compounds, sphaerococcinol A (4) and 14R-hydroxy-13,14-dihydro-sphaerococcinol A (5), were the most potent compounds without toxicity towards oyster larvae used as non-target organisms. These compounds are of high potential as they are active towards key biofoulers and could be produced by a cultivable alga, a fact that is important from the green chemistry point of view.

scholarly journals The Optimization of Secondary Metabolite Production Time and Screening Antibacterial Activity of Actinomycetes Isolate from Tin Plant Rizosfer (Ficus carica)

2018 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
Warsi Warsi ◽  
Nanik Sulistyani
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Incubation Time ◽  
Culture Fluid ◽  
Ficus Carica ◽  
Secondary Metabolite Production ◽  
Bacterial Isolates ◽  
Methicillin Resistant ◽  
Metabolite Production

Some Actinomycetes isolates of tin plant (Ficus carica L.) have been obtained, namely T24M, T18, T19, T24, T25, T34, T37, T41 and T43. The aim of this study were to optimize the production of secondary metabolites (antibiotics) and screening antibacterial activity against Methicillin Resistant Staphyllococcus aureus (MRSA) from the Actinomycetes isolate of the tin rhizosphere. The study was performed with test an activity of the culture fluid from Actinomycetes isolate against MRSA by the well method. The result of optimization secondary metabolite production showed that the second day was the best incubation time to harvest antibiotics. The results showed that bacterial isolates of T24M produced antibiotics that could inhibit MRSA growth.

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