scholarly journals New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoran Liu ◽  
Ruiyun Huo ◽  
Yanan Zhai ◽  
Ling Liu
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Mcf7 Cell ◽  
Cytotoxic Effects ◽  
Electronic Circular Dichroism

Three new secondary metabolites pestalothenins A–C (1–3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4–8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1–3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1–8 were also evaluated for antibacterial activity.

scholarly journals Chemical Constituents from the Fungus Antrodia cinnamomea

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Ming-Der Wu ◽  
Ming-Jen Cheng ◽  
Yen-Lin Chen ◽  
Hsun-Hsuo-Chang ◽  
Yueh-Hsiung Kuo ◽  
...  
Keyword(s):  
Solid State ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Cytotoxic Effects ◽  
Chemical Structures

A new benzenoid, 4-methoxy-7-methylbenzo[ d][1,3]dioxol-5-ol (1) and three known secondary metabolites 2,3-dimethoxy-5-methyl[1,4]benzoquinone (2), 2-methoxy-6-methyl-1,4-benzoquinone (3) and 5-methyl-benzo[1,3]dioxole-4,7-diol (4) were isolated from the mycelia of A. cinnamomea BCRC 36799 by solid state fermentation with adlay. Their chemical structures were elucidated on the basis of HRESIMS, NMR spectroscopic data and comparison with reported values. All isolated compounds 1–4 were tested for their cytotoxicity against the six cancer cell lines using the MTT assay. Among them, compound 3 displayed significant cytotoxic effects toward all six tested cancer cell lines, with IC50 values ranging from 2.8–8.7 μM in vitro.

scholarly journals Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense

2019 ◽  
Vol 14 (9) ◽  
pp. 1934578X1985790 ◽  
Author(s):  
Ming-Jen Cheng ◽  
Shuen-Shin Yang ◽  
Ming-Der Wu ◽  
Hsun-Hsuo Chang ◽  
Yueh-Hsiung Kuo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Lines ◽  
Endophytic Fungus ◽  
Structure Elucidation ◽  
Soluble Fraction ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Cytotoxic Effects ◽  
Mcf 7

Fractionation of the EtOAc-soluble fraction of the liquid fermentation of an endophytic fungus Annulohypoxylon ilanense associated with the wood of medicinal plant Cinnamomum species resulted in the isolation of 1 new α-pyrone, ilanpyrone (1), along with 3 previously identified compounds, 2 to 4. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, especially 1D and 2D nuclear magnetic resonance (NMR) spectroscopy as well as high-resolution electrospray ionization mass spectrometry. The isolated compounds were tested for their cytotoxicity against MCF-7, NCI-H460, and SF-268 cell lines using the MTT assay. Among them, compounds 1 and 4 were found to have moderate-to-weak cytotoxic effects against these 3 cell lines in vitro.

scholarly journals Pestalotilide A, a New Cytotoxic Diphenyl Ether Derivative from Pestalotiposis guepinii

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Jian Xiao ◽  
Wan-Tong Zhong ◽  
Li-Bin Lin ◽  
Xiang Zhao ◽  
Wei Shi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Diphenyl Ether ◽  
2D Nmr ◽  
Ether Derivative ◽  
Data Analyses ◽  
Organic Extracts

A new diphenyl ether derivative, named pestalotilide A (1), along with four known compounds (2-5) have been isolated from the organic extracts of an endophytic fungus Pestalotiposis guepinii. Their structures were elucidated on the basis of HR-ESI-MS, 1D and 2D NMR spectroscopic data analyses. These isolates were evaluated for cytotoxicity against three cancer cell lines: Hela, HCT116 and A549; pestalotilide A exhibited weak in vitro anti-proliferative activities against the tested cell lines, with IC50 values of 23.8, 18.4 and 35.2 μM, respectively.

In Vitro Evaluation of Chitosan Induced Cytotoxicity against Cancerous Cell lines: MCF-7, Saos-2 and HeLa

2019 ◽  
Vol 19 ◽  
Author(s):  
Zeinab Abedian ◽  
Niloofar Jenabian ◽  
Ali Akbar Moghadamnia ◽  
Ebrahim Zabihi ◽  
Roghayeh Pourbagher ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Fibroblast Cells ◽  
Apoptosis Induction ◽  
Cytotoxic Effects ◽  
Cancerous Cell ◽  
Significant Concentration ◽  
Mcf 7

Objective/ Background: Cancer is still the most common cause of morbidity in world and new powerful anticancer agents without severe side effects from natural sources is important. Methods: The evaluation of cytotoxicity and apoptosis induction was carried out in MCF-7,HeLa and Saos-2 as cancerous cell lines with different histological origin and human fibroblast served as control normal cell. The cells were treated with different concentrations of chitosan and the cytotoxicity was determined using MTT assay after 24, 48 and 72 h .The mode of death was evaluated by flow cytometry . Results: While both types of chitosan showed significant concentration-dependently cytotoxic effects against the three cancerous cell lines, fibroblast cells showed somehow more compatibility with chitosan. On the other hand, there were no significant differences between LMWC and HMWC cytotoxicity in all cell lines. The flow cytometry results showed the apoptosis pattern of death more in Saos-2 and HeLa while necrosis was more observable with MCF7. Also higher viability with both types of chitosan was seen in fibroblast as normal cells Conclusion: Chitosan shows anticancerous effect against 3 cancerous cell lines, while it is compatible with normal diploid fibroblast cells. Furthermore, it seems that the molecular weight of chitosan does not affect its anticancerous property.

Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2187-2192
Author(s):  
Rashid Rahim Hateet ◽  
Zainab Alag Hassan ◽  
Abdulameer Abdullah Al-Mussawi ◽  
Shaima Rabeea Banoon
Keyword(s):  
Antibacterial Activity ◽  
Trichoderma Harzianum ◽  
Endophytic Fungus ◽  
Nitrogen Sources ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Metabolite Production ◽  
Surface Sterilization ◽  
Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

scholarly journals Selective cytotoxicity of 125I-labeled monoclonal antibody T101 in human malignant T cell lines

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 429-435
Author(s):  
E Boven ◽  
T Lindmo ◽  
JB Mitchell ◽  
PA Jr Bunn
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Specific Activity ◽  
Specific Radioactivity ◽  
High Specific Activity ◽  
Tumor Localization ◽  
Cytotoxic Effects ◽  
High Specific Radioactivity ◽  
T Cell Lines

The radiolabeled anti-T cell antibody T101 can be used for specific tumor localization, but unlabeled T101 produces limited cytotoxicity in patients. We thus studied the in vitro cytotoxic effects of T101 labeled with 125I, a radionuclide known for its short-range, high- linear-energy electrons. We showed that 125I-T101 could be readily prepared at high specific activity with high immunoreactivity. Human malignant T cell lines HUT 102, MOLT-4, and HUT 78 were found to differ in the number of T65 determinants (the antigen recognized by T101) and the sensitivity to external x-ray radiation, which were of significance for the cytotoxicity of 125I-T101 in vitro. The cytotoxic effects of 125I-T101 were also found to be dose dependent and increased with exposure time under frozen conditions. As controls, unlabeled T101 had no cytotoxic effect, while free Na 125I or the 125I-labeled irrelevant antibody 9.2.27 exerted minor cytotoxicity. In HUT 102 and MOLT-4, more than 3 logs' cell killing was achieved within four weeks. Because considerable cytotoxicity was demonstrated in vitro by 125I-T101 on T65- positive malignant cells, and because low-dose 111In-T101 can be used successfully for tumor localization, future trials using 125I-T101 at high specific radioactivity may improve therapeutic results in patients with T65-positive malignancies.

scholarly journals Antibacterial and Cytotoxicity Activities of Major Compounds from Tinospora cordifolia Willd. Growing on Mangifera indica L.

2019 ◽  
Vol 3 (4) ◽  
pp. 32-42
Author(s):  
Thongchai Taechowisan
Keyword(s):  
Antibacterial Activity ◽  
Cell Lines ◽  
Crude Extract ◽  
Hek293 Cell ◽  
Colorimetric Assay ◽  
Mangifera Indica ◽  
Tinospora Cordifolia ◽  
Cytotoxicity Activity ◽  
Stem Extract ◽  
Cytotoxicity Activities

Objective To investigate the major constituents of Tinosporacordifolia Willd. growing on Mangiferaindica, and to evaluate the efficacy of their antibacterial and cytotoxicity activities. Methods The ethanolic stem extract of T. cordifolia was subjected to silica gel 60 column chromatography, thin layer chromatography and medium pressure liquid chromatography for isolation of the major compounds. Identification of purified compounds was achieved by spectroscopic methods.. The crude extract and purified compounds were screened for their antibacterial and cytotoxicity properties using standard procedures. Results Two alkaloids were purified and identified as Magnoflorin (1) and Tembetarine (2). These compounds showed high antibacterial activity against Bacillus cereus and Staphylococcus aureus with both MIC (32-64 µg/ml) and MBC (128-256 µg/ml). The cytotoxicity activity of the purified compounds and crude extract was determined using MTT colorimetric assay against L929 and HEK293 cell lines. This showed weak cytotoxicity activity with IC50 values of 1162.24 to 2290.00 µg/ml and 1376.67 to 2585.06 µg/ml towards L929 and HEK293 cell lines, respectively. Conclusion The major compounds present in ethanolic stem extract of T. cordifolia growing on M. indica were extracted, purified and identified. This study suggests that these compounds exhibit great potential for antibacterial activity with weak cytotoxicity activity. They may be useful for their medicinal functions.

Eryngium billardieri Extract and Fractions Induces Apoptosis in Cancerous Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Samira Hasanbeiglu ◽  
Kamran Hosseini ◽  
Ommoleila Molavi ◽  
Parina Asgharian ◽  
Vahideh Tarhriz
Keyword(s):  
Cell Lines ◽  
Mcf7 Cell ◽  
Inhibitory Effect ◽  
P Value ◽  
Breast Cancers ◽  
Natural Habitats ◽  
Cytotoxic Effects ◽  
Soxhlet Apparatus ◽  
Mcf7 Cell Line ◽  
Cancerous Cells

Background: Eryngium is genus flowering plants in the Umbelliferae family having pharmacological properties such as anti-inflammatory and anti-diabetic. Given the nature of melanoma and breast cancers in recent years and the fact that the anti-cancer properties of Eryngium billardieri on mentioned cell lines have not been studied, the present study conducted to explore these properties. Objective: The mechanisms of cytotoxicity and apoptosis of aerial parts of various extracts and fractions of E. billardieri on cancerous cells and normal cells were investigated. Methods: Samples were collected from natural habitats, dried and then extracted by Soxhlet apparatus with solvents of n-Hex, DCM and methanol, respectively. The cytotoxic effects of the extracts were investigated by MTT method on MCF7, B16 and HFF-2 classes for 24 and 48 hours. Flowcytometry, was also used to investigate the mechanism of cytotoxicity and confirming by Real-time PCR of p53 and Bax genes, which codes apoptosis regulator proteins. Meanwhile, volatile compounds of extracts were identified by GC-MS method. Results: The obtained data showed that the n-Hex extract of E. billardieri on B-16 and MCF7 cell lines and dichromethane extract on MCF7 cell line had the most significant cytotoxic effect compared to DMSO control (p value <0.001). Our finding showed that the mechanism of n-Hex extract with 80% and 100% vlc fractions on B16 induced apoptotic compared to HFF-2 control cells, moreover, n-Hex extract and 80% vlc fraction on MCF7 was apoptotic. The major compounds of n-Hex, DCM and 80% and 100% fractions of n-Hex extract obtained from GC-MS are non-terpenoid. Conclusion: Non-terpenoids compounds of E. billardieri can be the responsible for showing the most cytotoxic effects on MCF7 and B16 cell lines with apoptotic mechanism and n-Hex extract had the most significant inhibitory effect on cancerous cells compared to the HFF-2 control cells by the mechanism of apoptosis.

scholarly journals Evaluation of Antibacterial Activity of Cymbopogon citratus Ethanolic Leaf Crude Extract against Streptococcus pneumoniae isolated from Kampala International University Teaching Hospital Western Campus, Uganda

2019 ◽  
Vol 7 (1) ◽  
pp. 31-38
Author(s):  
Ibrahim Ntulume ◽  
Ninsiima Victoria ◽  
Abubakar Sunusi Adam ◽  
Adamu Almustapha Aliero
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pneumoniae ◽  
Crude Extract ◽  
Clinical Isolates ◽  
University Teaching ◽  
Zone Of Inhibition ◽  
Cymbopogon Citratus ◽  
Alternative Intervention ◽  
Adults And Children

Streptococcus pneumoniae is the common cause of pneumonia, meningitis, bacteremia and Septicemia among adults and children worldwide. Resistance to antimicrobials agents has been reported among S. pneumoniae which necessitate the need for alternative intervention such as ethno-medicinal plants. Cymbopogon citratus is an ethno-medicinal plant which is known to have pharmacological activities including antibacterial activity. This study aimed at determining the in vitro antibacterial activity of C. citratus ethanolic leaves crude extract against clinical isolates of S.  pneumoniae. A fresh leaves of C. citratus were collected early in the morning; shed dried, pulverized and extracted using ethanol (96%) using standard extraction method. The antibacterial activity, Minimum Inhibitory and Minimum Bactericidal Concentrations of C. citratus ethanolic leaves crude extract were determined against clinical isolates of S. pneumoniae. C. citratus ethanolic leaves extract crude showed antibacterial activity against S. pneumoniae at 500mg/ml concentration with mean and standard deviation zone of inhibition (26.33 ± 1.53 mm) in comparison with that of 250mg/ml concentration which gave 20.33 ± 2.08 mm mean and standard zone of inhibition. The minimum inhibitory concentration of the plant crude extract against S. pneumoniae was 15.63 mg/ml while the minimum bactericidal concentration was 125mg/ml. The study found that C. citratus leaves ethanolic crude extract was active against S. pneumoniae. It is recommended that studies should be done focusing on isolation of specific phytochemicals of the C. citratus leaves crude extract and then determines their antibacterial activity against clinical isolates of S. pneumoniae. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 31-38

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