scholarly journals The Influence on LPS-Induced ROS Formation in Macrophages of Capelloside A, a New Steroid Glycoside from the Starfish Ogmaster capella

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Natalia V. Ivanchina ◽  
Alla A. Kicha ◽  
Timofey V. Malyarenko ◽  
Anatoly I. Kalinovsky ◽  
Ekaterina S. Menchinskaya ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Steroid Glycoside ◽  
Raw 264.7 ◽  
Oxygen Species ◽  
E Coli ◽  
Raw 264.7 Cell ◽  
Intracellular Ros ◽  
Ros Formation ◽  
Raw 264.7 Cell Line ◽  
Ros Reactive Oxygen Species

A new steroid glycoside, capelloside A (1), was isolated from the ethanolic extract of the starfish Ogmaster capella along with the previously known coscinasteroside B (2). The structures of 1 was elucidated by extensive NMR and ESI-MS techniques as (24 S)-24- O-(3- O-methyl-β-D-xylopyranosyl)-5α-cholestane-3β,6β,8,15α,24-pentol 15- O-sulfate (1). Compounds 1 and 2 decreased intracellular ROS (reactive oxygen species) levels in murine macrophages of the RAW 264.7 cell line at induction by endotoxic lipopolysaccharide (LPS) from E. coli by 60% and 41%, correspondingly.

scholarly journals Regulusosides A, B, and C, Three New Polyhydroxysteroid Glycosides from the Starfish Pentaceraster regulus

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Alla A. Kicha ◽  
Natalia V. Ivanchina ◽  
Timofey V. Malyarenko ◽  
Anatoly I. Kalinovsky ◽  
Pavel S. Dmitrenok ◽  
...  
Keyword(s):  
Side Chain ◽  
Raw 264.7 ◽  
Oxygen Species ◽  
Murine Macrophage ◽  
Reactive Oxygen Species Formation ◽  
Species Formation ◽  
Intracellular Ros ◽  
Immunomodulatory Properties ◽  
Chain Compounds ◽  
Ros Reactive Oxygen Species

Three new polyhydroxysteroid glycosides, regulusosides A-C (1-3) along with the previously known five steroid compounds (4-8) were isolated from the tropical starfish Pentaceraster regulus. The structures of compounds 1-3 were established by extensive NMR and ESI-MS techniques. New glycosides have the same aglycon, (24 S)-5α-cholestane-3β,5,6β,8,15α,24-hexol, and various disaccharide moieties: β-D-xylopyranosyl-(1→2)-3- O-methyl-α-L-arabinofuranosyl (1), β-D-xylopyranosyl-(1→2)-α-L-arabinofuranosyl (2), and 2- O-methyl-β-D-xylopyranosyl-(1→2)-3- O-methyl-α-L-arabinofuranosyl (3), attached at C-24 of the side chain. Compounds 1, 2 and 6 exhibit potential immunomodulatory properties, inducing intracellular ROS (reactive oxygen species) formation in the RAW 264.7 murine macrophage cells.

Branched chain amino Acids as in vitro and in vivo Anti-Oxidation Compounds

2021 ◽  
pp. 3899-3904
Author(s):  
Moath Alqaraleh ◽  
Violet Kasabri ◽  
Ibrahim Al-Majali ◽  
Nihad Al-Othman ◽  
Nihad Al-Othman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Amino Acids ◽  
Branched Chain Amino Acids ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Branched Chain ◽  
Raw 264.7 Cell Line

Background and aims: Branched chain amino acids (BCAAs) can be tightly connected to metabolism syndrome (MetS) which can be counted as a metabolic indicator in the case of insulin resistance (IR). The aim of this study was to assess the potential role of these acids under oxidative stress. Material and Methods: the in vitro antioxidant activity of BCAAs was assessed using free radical 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays. For further check, a qRT-PCR technique was madefor detection the extent of alterations in gene expression of antioxidative enzymes (catalase and glutathione peroxidase (Gpx)) in lipopolysaccharides (LPS(-induced macrophages RAW 264.7 cell line. Additionally, BCAAs antioxidant activity was evaluated based on plasma H2O2 levels and xanthine oxidase (XO) activity in prooxidative LPS-treated mice. Results: Different concentrations of BCAAs affected on DPPH radical scavenging activity but to lesser extent than the ascorbic acid. Besides, BCAAs obviously upregulated the gene expression levels of catalases and Gpx in LPS-modulated macrophage RAW 264.7 cell line. In vivo BCAAs significantly minimized the level of plasma H2O2 as well as the activity of XO activity under oxidative stress. Conclusion: our current findings suggest that BCAAs supplementation may potentially serve as a therapeutic target for treatment of oxidative stress occurs with atherosclerosis, IR-diabetes, MetS and tumorigenesis.

scholarly journals Exposure to particle debris generated from passenger and truck tires induces different genotoxicity and inflammatory responses in the RAW 264.7 cell line

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222044 ◽  
Author(s):  
Anna Poma ◽  
Giulia Vecchiotti ◽  
Sabrina Colafarina ◽  
Osvaldo Zarivi ◽  
Lorenzo Arrizza ◽  
...  
Keyword(s):  
Cell Line ◽  
Inflammatory Responses ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Truck Tires ◽  
Raw 264.7 Cell Line

Winter urban air particles from Rome (Italy): Effects on the monocytic–macrophagic RAW 264.7 cell line

2005 ◽  
Vol 99 (3) ◽  
pp. 344-354 ◽  
Author(s):  
Roberta Pozzi ◽  
Barbara De Berardis ◽  
Luigi Paoletti ◽  
Cecilia Guastadisegni
Keyword(s):  
Cell Line ◽  
Urban Air ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Raw 264.7 Cell Line

Exposure to radiation from single or combined radio frequencies provokes macrophage dysfunction in the RAW 264.7 cell line

2018 ◽  
Vol 94 (6) ◽  
pp. 607-618 ◽  
Author(s):  
Alberto López-Furelos ◽  
Aarón A. Salas-Sánchez ◽  
Francisco J. Ares-Pena ◽  
José M. Leiro-Vidal ◽  
Elena López-Martín
Keyword(s):  
Cell Line ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Macrophage Dysfunction ◽  
Radio Frequencies ◽  
Raw 264.7 Cell Line

scholarly journals A New Bithiophene from the Root of Echinops grijsii

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Fang-Pin Chang ◽  
Chien-Chih Chen ◽  
Hui-Chi Huang ◽  
Sheng-Yang Wang ◽  
Jih-Jung Chen ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Cell Line ◽  
Natural Source ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Anti Inflammatory ◽  
First Time ◽  
Raw 264.7 Cell Line

A new bithiophene, 5-(4-hydroxy-3-methoxy-1-butyny)-2,2′-bithiophene (1), and sixteen known thiophenes: 2-(3,4-dihydroxybut-1-ynyl)-5-(penta-1,3-diynyl)thiophene (2), α-terthienyl (3), 5-(3,4-dihydroxybut-1-ynyl)-2,2′-bithiophene (4), 5-acetyl-2,2′-bithiophene (5), 5-formyl-2,2′-bithiophene (6), methyl 2,2′-bithiophene-5-carboxylate (7), 5-(but-3-en-1-ynyl)-2,2′-bithiophene (8), 5-(4-isovaleroyloxybut-1-ynyl)-2,2′-bithiophene (9), cardopatine (10), isocardopatine (11), 5-(3-hydroxy-4-isovaleroyloxybut-1-ynyl)-2,2′-bithiophene (12), 5-(3-hydroxymethyl-3-isovaleroyloxyprop-1-ynyl)-2,2′-bithiophene (13), 5-(4-hydroxy-1-butynyl)-2,2′-bithiophene (14), 5-(4-acetoxy-1-butynl)-2,2′-bithiophene (15), 2,2′-bithiophene-5-carboxylic acid (16) and 2-(4-hydroxybut-1-ynyl)-5-(penta-1,3-diynyl)thiophene (17) were isolated from the roots of Echinops grijisii Hance. Among them, compounds 6, 7 and 16 were isolated from a natural source for the first time. Compounds 2, 4 and 14 exhibited significant anti-inflammatory activity against nitrite of LPS-stimulated production in the RAW 264.7 cell line.

A far-red to NIR emitting ultra-sensitive probe for the detection of endogenous HOCl in zebrafish and the RAW 264.7 cell line

2019 ◽  
Vol 17 (14) ◽  
pp. 3538-3544 ◽  
Author(s):  
Vijay Natarajan ◽  
Natesan Thirumalaivasan ◽  
Shu-Pao Wu ◽  
Velmathi Sivan
Keyword(s):  
Cell Line ◽  
Fluorescence Imaging ◽  
Raw 264.7 ◽  
Raw 264.7 Cell ◽  
Endogenous Fluorescence ◽  
Line P ◽  
Raw 264.7 Cell Line

We report the synthesis of a far-red to NIR emitting probe for its application to the endogenous fluorescence imaging of HOCl in zebrafish.

Intracellular generation of reactive oxygen species in endothelial cells exposed to anoxia-reoxygenation

1997 ◽  
Vol 272 (5) ◽  
pp. L897-L902 ◽  
Author(s):  
J. J. Zulueta ◽  
R. Sawhney ◽  
F. S. Yu ◽  
C. C. Cote ◽  
P. M. Hassoun
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intracellular Ros ◽  
Ros Formation ◽  
Oxide Synthase

Reactive oxygen species (ROS) play an important role in the pathogenesis of ischemia-reperfusion injury. Extracellular H2O2 generation from bovine pulmonary artery endothelial cells (EC) is known to increase in response to anoxia-reoxygenation (A-R). To determine potential sources of intracellular ROS formation in EC in response to A-R, a fluorometric assay based on the oxidation of 2',7'-dichlorofluorescin was used. Intracellular ROS production declined 40% during 6 h of anoxia (P < 0.05). After A-R, the rates of intracellular ROS formation increased to 148 +/- 9% (P < 0.001) that of normoxic EC (100 +/- 3%). In EC exposed to A-R, allopurinol and NG-methyl-L-arginine (L-NMMA), inhibitors of xanthine oxidase (XO) and nitric oxide synthase (NOS), respectively, reduced intracellular ROS formation by 25 +/- 1% (P < 0.001) and 36 +/- 4% (P < 0.01). Furthermore, at low doses (i.e., 20 microM), deferoxamine and diethylenetriaminepentaacetic acid (DTPA) significantly inhibited intracellular ROS formation. However, at 100 microM, only deferoxamine caused further reduction in DCF fluorescence. In summary, EC respond to A-R by generating increased amounts of XO- and NOS-derived intracellular ROS. The inhibition, to a similar extent, caused by allopurinol and L-NMMA, as well as the effect of deferoxamine and DTPA suggest that the ROS detected is peroxynitrite. Based on these findings and previous work, we conclude that EC generate ROS in response to A-R from at least two different sources: a plasma membrane-bound NADPH oxidase-like enzyme that releases H2O2 extracellularly and XO, which generates intracellular O2-, which in turn may react with nitric oxide to form peroxynitrite.

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