scholarly journals Phytochemical and Biological Investigations of Conradina Canescens

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100
Author(s):  
Noura S. Dosoky ◽  
Debra M. Moriarity ◽  
William N. Setzer
Keyword(s):  
Ir Spectroscopy ◽  
Crude Extract ◽  
Spectroscopic Studies ◽  
Chloroform Extract ◽  
Phytochemical Study ◽  
Preliminary Screening ◽  
Biological Studies ◽  
Toxicity Analysis ◽  
Antileishmanial Activities ◽  
First Time

Conradina canescens (Lamiaceae) is an endemic evergreen shrub native to Florida, Mississippi and Alabama, with no phytochemical or biological studies registered in the literature. Thus, a phytochemical study and a toxicity analysis of the chloroform extract obtained from the leaves of C. canescens were performed for the first time. In our preliminary screening, the crude extract and its fractions were subjected to cytotoxicity, antimicrobial and antileishmanial bioassays. The crude extract showed substantial cytotoxic, antimicrobial and antileishmanial activities. A total of six compounds, namely ursolic acid (62.4%), betulin (8.4%), β-amyrin (4.6%), myrtenic acid (2.9%), n-tetracosane (1.4%), and oleanolic acid (1.1%), were isolated. The structures of the isolated compounds were established by spectroscopic studies using NMR and IR spectroscopy.

scholarly journals Phytochemical constituents and preliminary toxicity evaluation of leaves from Rourea induta Planch. (Connaraceae)

2011 ◽  
Vol 47 (3) ◽  
pp. 635-642 ◽  
Author(s):  
Milena Kalegari ◽  
Marilis Dallarmi Miguel ◽  
Josiane de Fátima Gaspari Dias ◽  
Ana Luísa Lacava Lordello ◽  
Cristina Peitz de Lima ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Toxicity Evaluation ◽  
Phytochemical Study ◽  
H Nmr ◽  
Biological Studies ◽  
Toxicity Analysis ◽  
High Doses ◽  
Toxic Potential ◽  
First Time

Most active plants are toxic at high doses and it is therefore important to investigate the preliminary toxicity of plant extracts. The Rourea induta species is a potential drug with no phytochemical or biological studies registered in the literature. Thus, a phytochemical study and a toxicity analysis of the ethanolic extract obtained from the leaves of Rourea induta Planch., Connaraceae, was run. A long chain hydrocarbon, n-tetracosane, and four flavonoids were identified: quercetin, and three glycosylated derivates, quercetin-3-O-α-arabinofuranoside, quercetin-3-O-β-xyloside and quercetin-3-O-β-galactoside. This is the first time these have been isolated in this species. The structures were elucidated by 13C NMR, ¹H NMR, UV and IR spectroscopy. The toxicity evaluation of extracts was performed by the brine shrimp method and determination of hemolytic activity. The samples demonstrated no toxic potential by the analyzed methods.

scholarly journals Phytochemical Profile and Anti-lipase Activity of Balkan Endemic Jurinea tzar-ferdinandii

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Antoaneta Trendafilova ◽  
Milka Todorova ◽  
Nikolina Kutova ◽  
Maya Guncheva
Keyword(s):  
Potent Inhibitor ◽  
Sesquiterpene Lactones ◽  
Candida Rugosa ◽  
Structural Identification ◽  
Chloroform Extract ◽  
Phytochemical Study ◽  
Phytochemical Profile ◽  
Inhibitory Potential ◽  
The Individual ◽  
First Time

Phytochemical study of the Balkan endemic J. tzar-ferdinandii Davidov allowed the identification of twenty-two components. β-Amyrin, lupeol, taraxasterol and Ψ-taraxasterol and their 3- O-acetates and 3- O-palmitates were identified by GC/MS. Six closely related sesquiterpene lactones and four flavones were isolated from the chloroform extract using column chromatography, and their structural identification was performed by spectral analyses. All isolated compounds are described for the first time in Jurinea species. The inhibitory potential of the total chloroform extract, fractions containing triterpenes, flavonoids and sesquiterpene lactones as well as individual compounds against a bacterial lipase from Candida rugosa (CRL) and a lipase from porcine pancrease (PPL) was evaluated. The half maximal inhibitory constants (IC50) for the two enzymes were in the range of 28–150 μg/mL. The strongest inhibitory activity was found for the total chloroform extract (29±1 μg/mL and 39±1 μg/mL for CRL and PPL, respectively.). Among the individual compounds, onopordopicrin was found to be the most potent inhibitor for CRL and PPL with IC50 values of 32±1 μg/mL and 36±1 μg/mL, respectively.

scholarly journals Terpenoids in four Inula species from Bulgaria

2021 ◽  
pp. 87-87
Author(s):  
Antoaneta Trendafilova ◽  
Victoria Ivanova ◽  
Milka Todorova ◽  
Plamena Staleva ◽  
Ina Aneva
Keyword(s):  
Principal Component Analysis ◽  
Sesquiterpene Lactones ◽  
Principal Component ◽  
Component Analysis ◽  
Chloroform Extract ◽  
Spectral Analyses ◽  
Phytochemical Study ◽  
Aerial Parts ◽  
First Time

Phytochemical study of the chloroform extract of the aerial parts of Inula germanica L., I. ensifolia L., I. conyza (Griess.) DC. and I. salicina L. led to the identification of 33 terpenoids. ?- and ?-Amyrin, lupeol, taraxasterol, ?-taraxasterol and their 3-O-acetates and 3-O-palmitates were identified by GC/MS. In addition, the structures of 3-O-palmitates of mainaladiol, arnidiol, faradiol and 16-hydroxylupeol were confirmed by NMR. ent-kaur-16-en-19-oic acid and its 15?-(3-methylpentanoyloxy) and 15?-(3-methylbutanoyloxy) deriva-tives were isolated from I. conyza. Ten closely related sesquiterpene lactones (germacranolides and melampolides) were found in I. germanica and their struc-tural identification was performed by spectral analyses. I. ensifolia and I. salicina were free of sesquiterpene lactones and diterpenoids. All triterpenoids and diterpenoids, grazielia acid, desacetylovatifolinan, and 8-(2-methylbutanoyloxy)- -1(10),4,11(13)-germacrutrien-6,12-olide-14-oic acid are described for the first time in the studied species. Principal component analysis (PCA) was used to find a relationship between investigated up to now Inula species, growing in Bulgaria.

scholarly journals INVESTIGATION OF ANTIMICROBIAL ACTIVITY AND CHEMICAL CONSTITUENTS OF MOMORDICA CHARANTIA L. VAR. ABBREVIATA SER.

2019 ◽  
Vol 57 (2) ◽  
pp. 155 ◽  
Author(s):  
Tram Thi Thu Nguyen ◽  
Dien Minh Vo ◽  
Thanh Huynh Vu Luong ◽  
Hien Thi Thanh Phan ◽  
Dam Phuc Nguyen
Keyword(s):  
Chemical Constituents ◽  
2D Nmr ◽  
Chloroform Extract ◽  
Momordica Charantia ◽  
Phytochemical Study ◽  
Dilution Assay ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
First Time ◽  
Microbroth Dilution

The antimicrobial potential of the four extracts of Momordica charantia L. var. abbreviata Ser was screened against four bacterial and four fungal strains, using microbroth dilution assay. The chloroform extract showed the highest growth inhibitory activity. Phytochemical study on the bioactive chloroform extract led to the isolation of four known compounds as octadecan-1-ol (1), (23E)-5β,19-epoxycucurbita-6,23,25-trien-3β-ol (2), 5α-poriferasta-7,25-dien-3β-ol (3) and 3-O-(6′-O-palmitoyl-β-D-glucopyranosyl)-clerosterol (4). Their structures were elucidated by spectroscopic methods including 1D and 2D-NMR, MS and in comparison with literature data. Here, such metabolites were reported for the first time in M. charantia L. var. abbreviata Ser.

Green Synthesis and Spectroscopic Studies of Ag-rGO Nanocomposites for Highly Selective Mercury (II) Sensing

2018 ◽  
Vol 9 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Shubhangi J. Mane-Gavade ◽  
Sandip R. Sabale ◽  
Xiao-Ying Yu ◽  
Gurunath H. Nikam ◽  
Bhaskar V. Tamhankar
Keyword(s):  
Green Synthesis ◽  
Color Change ◽  
Limit Of Detection ◽  
Aqueous Media ◽  
Suitable Condition ◽  
Cost Effective ◽  
Spectroscopic Studies ◽  
Calibration Plot ◽  
First Time

Introduction: Herein we report the green synthesis and characterization of silverreduced graphene oxide nanocomposites (Ag-rGO) using Acacia nilotica gum for the first time. Experimental: We demonstrate the Hg2+ ions sensing ability of the Ag-rGO nanocomposites form aqueous medium. The developed colorimetric sensor method is simple, fast and selective for the detection of Hg2+ ions in aqueous media in presence of other associated ions. A significant color change was noticed with naked eye upon Hg2+ addition. The color change was not observed for cations including Sr2+, Ni2+, Cd2+, Pb2+, Mg2+, Ca2+, Fe2+, Ba2+ and Mn2+indicating that only Hg2+ shows a strong interaction with Ag-rGO nanocomposites. Under the most suitable condition, the calibration plot (A0-A) against concentration of Hg2+ was linear in the range of 0.1-1.0 ppm with a correlation coefficient (R2) value 0.9998. Results & Conclusion The concentration of Hg2+ was quantitatively determined with the Limit of Detection (LOD) of 0.85 ppm. Also, this method shows excellent selectivity towards Hg2+ over nine other cations tested. Moreover, the method offers a new cost effective, rapid and simple approach for the detection of Hg2+ in water samples.

The Anticholinesterase Activity of Three Local Food Spices and Their Anti-Alzheimer Application

2020 ◽  
Vol 01 ◽  
Author(s):  
Ruqia Bibi ◽  
Saima Gul ◽  
Abdul Wahab ◽  
Mohammad Iqbal Khan ◽  
Murad Ali Khan
Keyword(s):  
Neurodegenerative Disorder ◽  
Curcuma Longa ◽  
Chloroform Extract ◽  
Food Plants ◽  
In Vivo Studies ◽  
Assay Method ◽  
Inhibiting Activity ◽  
Asian Countries ◽  
Phytochemical Study ◽  
The World

Background: The incidence of dementia is increasing as the aging population of the world is increasing. Alzheimer’s disease (AD) is the neurodegenerative disorder of the central nervous system. There are presently 7.3 million patents of AD and the number may rise to 34 million at this pace in the coming thirty years. In the disease, the level of Acetylcholine is reduced and as a result, causes the loss of cholinergic neurons in the brain. The disease is less common in Asian countries as compared to the western nations of the world. This work aimed to establish the role of the common medicinal and food plants against Alzheimer. Methods: The enzyme acetylcholinesterase (AChE) is the enzyme responsible for hydrolysis and reduction of Acetylcholine. The anti-acetylcholinesterase activity of different extracts of three local plants used as spices in the daily food, Curcuma longa, Cinnnamomum tamala, and Zingiber officinale, were determined using the Microplate Assay method. Results: The phytochemical study of the selected plants revealed the presence of alkaloids, terpenes, flavones, saponins, and tannins in these plants. The chloroform extract of all the three plants presented promising AChE inhibiting activity having IC50 >200μg/ml. A probable reason will be the alkaloids and terpenes present in the chloroform extract. Conclusion: The chloroform extract of all three plants presented promising AChE inhibiting activity and can become a reasonable therapy for the cure/ prevention of Alzheimer disease. The frequent use of these spices may be a possible reason for the fever incidence of Alzheimer in Asian countries. Further in vivo studies are required to find its action and studies to find the exact compound responsible for the action.

scholarly journals Antifungal Activity of Chemical Constituents from Piper pesaresanum C. DC. and Derivatives against Phytopathogen Fungi of Cocoa

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3256
Author(s):  
Luis C. Chitiva-Chitiva ◽  
Cristóbal Ladino-Vargas ◽  
Luis E. Cuca-Suárez ◽  
Juliet A. Prieto-Rodríguez ◽  
Oscar J. Patiño-Ladino
Keyword(s):  
Antifungal Activity ◽  
Benzoic Acid ◽  
Chemical Constituents ◽  
Chemical Study ◽  
Positive Control ◽  
Positive Influence ◽  
Phytopathogenic Fungi ◽  
Phytochemical Study ◽  
Benzoic Acid Derivatives ◽  
First Time

In this study, the antifungal potential of chemical constituents from Piper pesaresanum and some synthesized derivatives was determined against three phytopathogenic fungi associated with the cocoa crop. The methodology included the phytochemical study on the aerial part of P. pesaresanum, the synthesis of some derivatives and the evaluation of the antifungal activity against the fungi Moniliophthora roreri, Fusarium solani and Phytophthora sp. The chemical study allowed the isolation of three benzoic acid derivatives (1–3), one dihydrochalcone (4) and a mixture of sterols (5–7). Seven derivatives (8–14) were synthesized from the main constituents, of which compounds 9, 10, 12 and 14 are reported for the first time. Benzoic acid derivatives showed strong antifungal activity against M. roreri, of which 11 (3.0 ± 0.8 µM) was the most active compound with an IC50 lower compared with positive control Mancozeb® (4.9 ± 0.4 µM). Dihydrochalcones and acid derivatives were active against F. solani and Phytophthora sp., of which 3 (32.5 ± 3.3 µM) and 4 (26.7 ± 5.3 µM) were the most active compounds, respectively. The preliminary structure–activity relationship allowed us to establish that prenylated chains and the carboxyl group are important in the antifungal activity of benzoic acid derivatives. Likewise, a positive influence of the carbonyl group on the antifungal activity for dihydrochalcones was deduced.

scholarly journals Chemical and biological studies on the soft coral Nephthea sp.

RSC Advances ◽  
2021 ◽  
Vol 11 (38) ◽  
pp. 23654-23663
Author(s):  
Omnia Hesham Abdelhafez ◽  
John Refaat Fahim ◽  
Ramy R. El Masri ◽  
M. Alaraby Salem ◽  
Samar Yehia Desoukey ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Crude Extract ◽  
Soft Coral ◽  
Cytotoxic Potential ◽  
Esi Ms ◽  
Biological Studies ◽  
Metabolomics Analysis

The cytotoxic potential of the crude extract, different fractions, and green synthesized nanoparticles of the soft coral Nephthea sp. was studied, supported by LC-HR-ESI-MS metabolomics analysis and molecular docking of the dereplicated compounds.

scholarly journals In vitro growth-inhibitory effect of Brazilian plants extracts against Paenibacillus larvae and toxicity in bees

2015 ◽  
Vol 87 (2) ◽  
pp. 1041-1047 ◽  
Author(s):  
Mariana Piana ◽  
Thiele F. de Brum ◽  
Aline A. Boligon ◽  
Camilla F.S. Alves ◽  
Robson B. de Freitas ◽  
...  
Keyword(s):  
Crude Extract ◽  
Inhibitory Effect ◽  
Paenibacillus Larvae ◽  
Dilution Method ◽  
Minimal Inhibitory Concentrations ◽  
Application Method ◽  
Growth Inhibitory ◽  
Toxicity Analysis ◽  
Spreading Disease

American foulbrood (AFB) is a serious worldwide spreading disease in bees caused by Paenibacillus larvae. Plants extracts are known to decrease or inhibit the growth of these bacteria. The purpose of this study was to evaluate the antimicrobial activity of Calendula. officinalis, Cariniana domestica, and Nasturtium officinale extracts against the P. larvae and to evaluate the toxicity of the extracts in bees. In vitro activity against P. larvae of the extracts was evaluated by micro dilution method and the minimal inhibitory concentrations (MICs) were also determined. The concentrations used in the toxicity test were established based on the MIC values and by the spraying application method. The P. larvae was susceptible to the evaluated crude extract of C. officinalis and N. officinale. To C. domestica, only the ethyl acetate (EtAc) fraction and n-butanol (BuOH) fractions had activity against P. larvae. Toxicity analysis in bees showed no toxicity for N. officinale crude extract and for C. domestica BuOH fraction during 15 days of treatment, however, some deaths of bees occurred during the first three days of treatment with C. officinalis and C. domestica EtAc fraction. The results with these species were firstly described and showed that N. officinale crude extract and C. domestica BuOH fraction both presented not toxic effects in the concentration tested by the spraying application method, and can be a useful alternative for treatment or prevention of AFB.

Enzyme Inhibiting Terpenoids from Amberboa ramosa

2004 ◽  
Vol 59 (5) ◽  
pp. 579-583 ◽  
Author(s):  
Sher B. Khan ◽  
Abdul Malik ◽  
Nighat Afza ◽  
Nusrat Jahan ◽  
Azhar U. Haq ◽  
...  
Keyword(s):  
2D Nmr ◽  
Spectroscopic Studies ◽  
Inhibitory Potential ◽  
First Time ◽  
Amberboa Ramosa

Abstract Two new cycloartane type triterpenes 1 and 2 have been isolated from Amberboa ramosa. Their structures were established as (22R)-cycloart-20, 25-dien-2α,3β ,22α-triol 1 and (22R)-cycloart-23- ene-3β ,22α, 25-triol 2 through spectroscopic studies including 2D-NMR. The known compounds 3,4-epoxyguaia-1(10), 11(13)-dien-6, 12-olide 3, and 5-hydroxy 7,8,2’,3’-tetramethoxyflavone 4 are also reported for the first time from this species. The compounds 1 and 2 displayed inhibitory potential against butyrylcholinesterase.

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