scholarly journals Plant Tissue Cultures of Juniperus virginiana

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Marie Kašparová ◽  
Jiřina Spilková ◽  
Ladislav Cvak ◽  
Tomáš Siatka ◽  
Jan Martin
Keyword(s):  
Ascorbic Acid ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Juniperus Virginiana ◽  
Naphthaleneacetic Acid ◽  
Tissue Cultures ◽  
Maximum Biomass ◽  
Cultivation Period ◽  
Plant Tissue Cultures ◽  
One Year

Callus cultures of Juniperus virginiana L. (varieties ‘Hetzii’, ‘Glauca’, ‘Grey Owl’) were derived from fresh leaves of garden-grown trees on Schenk and Hildebrandt medium supplemented with 3.0 mg/L of α-naphthaleneacetic acid, 0.2 mg/L of kinetin and 15 mg/L of ascorbic acid. The growth characteristics of one-year-old and two-years-old cultures were determined. The maximum biomass in all varieties was achieved on the 35th day of the cultivation period. The increase in fresh weights of two-years-old callus cultures, when compared with one-year-old callus cultures, was as follows: variety ‘Hetzii’ by 25%, variety ‘Glauca’ by 29% and variety ‘Grey Owl’ by 49%. J. virginiana suspension cultures (varieties ‘Hetzii’, ‘Glauca’, ‘Grey Owl’) were derived from two-years-old callus cultures on Schenk and Hildebrandt medium supplemented with 3.0 mg/L of α-naphthaleneacetic acid, 0.2 mg/L of kinetin and 15 mg/L of ascorbic acid. The maximum biomass of all varieties was found on the 21st day of the cultivation period. These results indicate that a sub-cultivation interval of 35 days for callus cultures and of 21 days for suspension cultures can be recommended. The callus and suspension cultures of J. virginiana of the variety ‘Glauca’ have the best survivability and thus provide the most biomass.

scholarly journals Production of Podophyllotoxin by Plant Tissue Cultures of Juniperus virginiana

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Marie Kašparová ◽  
Jan Martin ◽  
Lenka Tůmová ◽  
Jiřina Spilková
Keyword(s):  
Plant Tissue ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Juniperus Virginiana ◽  
Tissue Cultures ◽  
Potential Source ◽  
Plant Tissue Cultures ◽  
One Year ◽  
Maximum Content ◽  
Biogenetic Precursor

Plant tissue cultures are a potential source of secondary metabolites. However, their production, when compared with intact plants, is usually lower. Phenylalanine, a biogenetic precursor of podophyllotoxin, was used to stimulate podophyllotoxin production in callus and suspension cultures of Juniperus virginiana L. The best phenylalanine effect on podophyllotoxin production was manifested in three-years-old callus cultures after a 21-days application of a 10 mmol/L concentration. A podophyllotoxin content of 0.15 mg/g DW was determined, which was about 400% higher in comparison with the control. The maximum content (0.48 mg/g DW) in newly derived suspension cultures (the 4th passage) was induced by 14-days application of a 1 mmol/L concentration; this was about 243% higher than the control. In one-year-old suspension cultures the highest podophyllotoxin content (0.56 mg/g DW) was recorded also after 14-days application of a 1 mmol/L concentration; this was about 211% higher than in the control cultures.

Effects of Plant Bioregulators on the Production of Iridoid Derived Terpenoids in Valeriana wallichii and Fedia cornucopiae Cell Suspension Cultures

1987 ◽  
Vol 42 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
Wolfram Förster ◽  
Hans Becker
Keyword(s):  
Cell Suspension ◽  
Cell Cultures ◽  
Exponential Growth ◽  
Cell Suspension Cultures ◽  
Growth Cycle ◽  
Suspension Cultures ◽  
Tissue Cultures ◽  
Cell Systems ◽  
Optimal Activity ◽  
Plant Tissue Cultures

Abstract Four plant bioregulators were tested for their effects on production of valepotriates in Valeriana wallichii and Fedia cornucopiae cell suspension cultures. Concentrations of more than 10 ppm reduced valepotriate yield. At lower concentrations production was increased. For optimal activity, bioregulators had to be applied during early exponential growth, up to day 8 of the growth cycle. At equimolar concentrations dim ethylm orpholinium bromide (4 ppm) and dimethylpiperidinium chloride (3 ppm) significantly im proved total valepotriates in V. wallichii (up to 23%) and in F cornucupiae (up to 50% ) 2-(3,4-dichlorophenoxy ) - triethylamine (6 ppm ) and 2-(3,5-diisopropylphenoxy)-triethylam ine (6.4 ppm) increased valepotriate production in both cell cultures up to 40%. With dimethylpiperidinium chloride and dimethylmorpholinium bromide the ratio of m onoene to diene valepotriates in both cell systems was significantly shifted to the m onoene com pounds. A general use of these bioregulators to increase production of terpenoid secondary m etabolites in plant tissue cultures is indicated.

GROWTH OF LETTUCE AND CAULIFLOWER TISSUES IN VITRO AND THEIR PRODUCTION OF ANTIMICROBIAL METABOLITES

1965 ◽  
Vol 11 (5) ◽  
pp. 785-789 ◽  
Author(s):  
Gordon Campbell ◽  
E. C. S. Chan ◽  
W. G. Barker
Keyword(s):  
Insoluble Fraction ◽  
Plant Tissues ◽  
Naphthaleneacetic Acid ◽  
Tissue Cultures ◽  
Mycobacterium Phlei ◽  
Proliferative Growth ◽  
Plant Tissue Cultures ◽  
Antimicrobial Metabolites ◽  
Medical Interest

Plant tissue cultures of cauliflower and lettuce were successfully established on semisolid White"s medium supplemented with coconut milk (20%) and naphthaleneacetic acid (5 p.p.m.). On this medium the tissues exhibited vigorous proliferative growth with no tendency for organogenesis. Ethanol–ether extracts of the stale medium of these tissues yielded an alcohol-insoluble fraction that was highly and consistently inhibitory for Staphylococcus aureus but variable in antimicrobial activity against Mycobacterium phlei. The results suggest that in view of the progress in mass tissue culturing of plant cells in vitro, the use of cultured plant tissues should be explored for the production of antimicrobial principles of medical interest.

In vitro culture of Bouvardia ternifolia

1982 ◽  
Vol 60 (6) ◽  
pp. 917-921 ◽  
Author(s):  
Leonor Fernandez ◽  
Estela Sanchez de Jimenez
Keyword(s):  
Indoleacetic Acid ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Napthaleneacetic Acid ◽  
Leaf Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

Callus cultures were induced from radicle and leaf tissues of Bouvardia ternifolia (trompetilla). Optimum growth regulator concentrations for radicle callus cultures were 1 mg/L 2,4-dichlorophenoxyacetic acid and 0.005 mg/L kinetin; for leaf callus they were either 2 mg/L naphthaleneacetic acid and 0.002 mg/L benzylaminopurine or 5 mg/L of idoleacetic acid and 0.01 mg/L kinetin. Callus has been maintained in culture for nearly 3 years with a very rapid growth rate.A generation time of approximately 24 to 28 h was obtained for batch cell suspension cultures. Production of protoplasts from suspension cultures was optimized with a yield of 70 to 90%. Protoplast culture was achieved in droplets of fresh medium with 2 mg/L napthaleneacetic acid, 0.01 mg/L benzylaminopurine, and0.5 M mannitol. After 2 years, callus in culture still retained its organogenetic capacity. An average of 18 complete plantlets from approximately 2 g of callus can be obtained after transfer to medium with 0.1 mg/L indoleacetic acid and 0.1 mg/L benzylaminopurine.

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

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