Seasonal Variation of Essential Oil in Rosmarinus officinalis Leaves in Sardinia

Rosmarinus officinalis L. is an aromatic plant belonging to the Lamiaceae family widely distributed in the Mediterranean area. The interest on this species is related to the multiple uses of the plant as a food ingredient, in the pharmaceutical and cosmetic industries. The chemical composition of essential oil (EO) from 5 accessions of R. officinalis L., collected monthly through a full year in Sardinia, has been studied by gas chromatography (GC) and GC-mass spectrometry technique. The EO ranged from 0.29% to 0.89%. The qualitative determinations revealed the presence of 27 compounds belonging to 6 chemical groups (hydrocarbon monoterpene, alcohols, aldehydes, ketones, esters, hydrocarbon sesquiterpene). Overall the GC-flame ionization detector analysis showed the presence of 7 major compounds: α-pinene (26%-28%), camphene (5%-8%), 1,8-cineole (15%-25%), borneol (5%-11%), camphor (3%-12%), verbenone (6%-15%), and bornyl acetate (4%-7%). Chromatographic data were also subjected to a chemometric approach that evidenced discrimination of the samples according to the site of collection.

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Rosmarinus officinalis L.: Chemical Modifications of the Essential oil and Evaluation of Antioxidant and Antimicrobial Activity

Natural Product Communications ◽

10.1177/1934578x0900401215 ◽

2009 ◽

Vol 4 (12) ◽

pp. 1934578X0900401 ◽

Cited By ~ 5

Author(s):

Giorgio Pintore ◽

Mauro Marchetti ◽

Mario Chessa ◽

Barbara Sechi ◽

Nadia Scanu ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Rosmarinus Officinalis ◽

Hydrocarbon Fraction ◽

Bornyl Acetate ◽

Minimal Bactericidal Concentration ◽

Aeromonas Sobria ◽

Antioxidant And Antimicrobial Activity ◽

Rosmarinus Officinalis L ◽

Micro Organisms

Rosmarinus officinalis essential oil was separated into its hydrocarbon and oxygenated fractions. The major compounds in the hydrocarbon fraction were α-pinene (44.2%), camphene (24.5%), and limonene (11.7%), while in the oxygenated fraction they were 1,8-cineole (37.6%), camphor (16.5%), and bornyl acetate (21.4%). The hydrocarbon fraction was submitted to a hydroformylation process and the antioxidant activity of the product was screened by the DPPH and β-carotene/linoleic acid tests. The hydroformylated fraction maintained the antioxidant activity of the whole oil. The MIC (minimal inhibitory concentration) and the MBC (minimal bactericidal concentration) of the essential oil, hydrocarbon, oxygenated and hydroformylated fractions were also tested on several microorganisms. Aeromonas sobria and Candida strains were the most susceptible micro-organisms. The hydroformylated fraction exhibited a MBC against Candida strains resistant to the other fractions.

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Effect of Salinity and Water Stress on the Essential Oil Components of Rosemary (Rosmarinus officinalis L.)

Agronomy ◽

10.3390/agronomy9050214 ◽

2019 ◽

Vol 9 (5) ◽

pp. 214 ◽

Cited By ~ 7

Author(s):

Radhia Sarmoum ◽

Soumia Haid ◽

Mohamed Biche ◽

Zahreddine Djazouli ◽

Bachar Zebib ◽

...

Keyword(s):

Water Stress ◽

Essential Oil ◽

Volatile Compounds ◽

Salt Water ◽

Tap Water ◽

Rosmarinus Officinalis ◽

Bornyl Acetate ◽

Oil Components ◽

Rosmarinus Officinalis L ◽

Water Treatments

The effect of salinity and water stresses on the essential oil components of Rosmarinus officinalis essential oil was investigated. Rosemary plants were submitted to different water treatments: tap water (TW), salt water (SW) and without irrigation (NIR). GC/MS analysis showed that ten and eleven volatile compounds were identified in essential oil of rosemary plants irrigated with tap water (TW) and salt water (SW), respectively. However, thirteen volatile compounds were identified in essential oil of non-irrigated plants (NIR). Moreover, among these compounds, α-Pinene, Eucalyptol (1,8 Cineol), Camphene, Borneol, D-verbenone, Bornyl acetate were the major components of oil. Also, GC/MS results highlighted that non-irrigated rosemary plants showed the highest essential oil yield (Y). Obtained oil yields followed the order YNIR > YTW > YSW. In conclusion, qualitative and quantitative differences in rosemary essential oil components were highlighted in relation to water stress.

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Physico-Chemical Characteristics of Rosmarinus officinalis L. Essential Oils Grown in Lam Dong Province, Vietnam

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.22166 ◽

2019 ◽

Vol 31 (12) ◽

pp. 2759-2762 ◽

Cited By ~ 14

Author(s):

Tran Thi Kim Ngan ◽

Nguyen Cam Huong ◽

Xuan Tien Le ◽

Pham Quoc Long ◽

Tran Quoc Toan ◽

...

Keyword(s):

Essential Oil ◽

Extraction Process ◽

Rosmarinus Officinalis ◽

Bornyl Acetate ◽

Chemical Characteristics ◽

Chemical Compositions ◽

Physico Chemical ◽

Cultivated Plant ◽

Geographical Position ◽

Rosmarinus Officinalis L

Composition of rosemary essential oil largely depends on the geographical position of the cultivated plant and conditions of the extraction process. In this study, fresh rosemary leaves were used for extraction of essential oil by hydrodistillation and evaluation of chemical compositions and physico-chemical characteristics of the obtained oil were performed. The yield of essential oil was 1.0 %. The physico-chemical parameters showed specific gravity (0.8978 g/cm3), acid index (1.122 mg KOH/g), ester index (15.708 mg KOH/g) and refractive index (1.464). Twenty three components were identified in Rosmarinus officinalis L. oil. The major components were α-pinene (35.54 %), eucalyptol (20.902 %), camphene (4.384 %), bicyclo[3.1.1]hept-3-en-2-one (7.794 %), caryophyllene (1.225 %), endo-borneol (4.147 %) and bornyl acetate (4.065 %). Present study unveiled differences in the chemical composition of Vietnamese rosemary oil comparing with similar studies carried out in other countries.

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Simultaneous Distillation–Extraction of Essential Oils from Rosmarinus officinalis L.

Cosmetics ◽

10.3390/cosmetics8040117 ◽

2021 ◽

Vol 8 (4) ◽

pp. 117

Author(s):

Bárbara Silva Ribeiro ◽

Maria de Fátima Ferreira ◽

José Luís Moreira ◽

Lúcia Santos

Keyword(s):

Essential Oils ◽

Solvent Mixture ◽

Rosmarinus Officinalis ◽

Bornyl Acetate ◽

Cuticular Waxes ◽

Extraction Solvent ◽

Flame Ionization ◽

Relative Standard ◽

Simultaneous Distillation Extraction ◽

Rosmarinus Officinalis L

The present study describes a procedure to isolate essential oils from Rosmarinus officinalis L. using simultaneous distillation–extraction (SDE). Rosmarinus officinalis L. can be used for medicinal purposes, as well in the cooking and cosmetics industries. SDE technique extraction combines a steam distillation combined with a continuous extraction using a solvent or a co-solvent mixture, providing faster extractions with low extraction solvent volumes. The effect of the solvent nature and the extraction time on the simultaneous distillation–extraction efficiency was evaluated. The best performance was achieved using pentane as a solvent for 1 h of extraction. The essential oils obtained by simultaneous distillation–extraction extracts were analyzed by gas chromatography with flame ionization detection (GC-FID). Extraction efficiencies ranged from 40 to 70% for the majority of the compounds tested, and the precision (measured by the relative standard deviation) varied between 6 and 35%. Among the compounds analyzed the most abundant in the Rosmarinus officinalis L. sample were 1,8-cineole, (-) –borneol, α-pinene, (S)-(-)- α–terpineol, (-)-bornyl acetate, linalool, and 2,2,6-trimethylcyclohexanone. The SDE method proved to be a suitable option for obtaining extracts free from cuticular waxes or chlorophylls.

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