Analysis of allergen profile in patients sensitized to canine allergen and potential Can f 5 cross-reactivity with human PSA

Can f 5 allergy and possible cross-reactivity with human semen in which there are significant amounts of prostate-specific antigen (PSA) are particularly interesting aspects of allergy to dog. The objective of the study was to confirm cross-reactivity between human PSA and Can f 5 in a study of canine sensitised women. A total of 100 women (aged 18–73, 41 on average) with a positive history of animal fur allergy or positive skin prick tests to canine allergens were selected. Levels of Immunoglobulin E (IgE) specific to Can f 1, Can f 2, Can f 3, Can f 5 were determined. Patients with increased concentration of sIgE Can f 5 were selected for further inhibition testing using polystyrene microplate ELISA test coated with human PSA. In the studied population, allergy to Can f 5 dominated (52.3% of patients with increased concentration of canine-specific IgE were allergic to this allergenic component). In all analyzed cases, the concentration of IgE Can f 5 decreased after incubation on the ELISA plate coated with human PSA. The minimum decrease in concentration was 10.44%, the maximum was 37.73%, the average decrease was 21.6%. No statistically significant influence of the presence or absence of allergenic sIgE Can f 5 in blood serum on the occurrence of symptoms after intercourse was found. The study confirmed the moderate ability of Can f 5 to cross-react with human PSA sIgE, which may be clinically significant in some women. At the same time, symptoms of an allergy to male semen do not constitute a typical clinical presentation of allergy to Can f 5.

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Utility of Shrimp and Der p 10 specific IgE for Shrimp Allergy Diagnosis in Non-House Dust Mite Sensitized Patients

10.1101/199620 ◽

2017 ◽

Cited By ~ 1

Author(s):

Karen Thursday S. Tuano ◽

Sara Anvari ◽

Imelda Celine Hanson ◽

Joud Hajjar ◽

Filiz Seeborg ◽

...

Keyword(s):

House Dust ◽

House Dust Mite ◽

Specific Ige ◽

Cross Reactivity ◽

Rank Test ◽

Positive Skin Prick Test ◽

Operating Characteristics ◽

Positive Skin ◽

Dust Mite ◽

History Of

ABSTRACTBackgroundThere are no set specific IgE (sIgE) to predict shrimp allergy as cross-reactivity with other arthropods play a role in shrimp sensitization.ObjectiveThis study identifies the allergens associated with shrimp allergy in house dust mite (HDM) and non-HDM sensitized patients.MethodsPatients with shrimp sensitization (positive skin prick test [SPT] and/or sIgE) with/without history of clinical reaction were recruited. Allergy was confirmed by oral food challenge (OFC) except for patients with history of anaphylaxis. Shrimp allergic (SA) and shrimp tolerant (ST) patients were further classified based on HDM sensitivity. The sIgE to shrimp, shrimp and HDM components were performed. Fisher’s exact test, Wilcoxon sum rank test and receiver operating characteristics analyses were done.ResultsOf 79 patients recruited, 12 SA (7 positive OFC and 5 with history of anaphylaxis), 18 ST and 10 non-shrimp sensitized controls (NC) were enrolled. In non-HDM sensitized patients, sIgE to shrimp (10.5 kUA/L, p=0.012) and Der p 10 (4.09 kUA/L, p=0.035) were higher in SA patients. Shrimp sIgE ≥3.55 kUA/L had 100% sensitivity and 85.71% specificity (ROC=0.94[0.81, 1.0]). Der p 10 sIgE ≥3.98 kUA/L had sensitivity of 80% and specificity of 100% (ROC=0.86[0.57, 1.0]). rPen a 1 ≥1.1 kUA/L had sensitivity of 80% and specificity of 85.7% (ROC=0.80[0.47,1.0]).ConclusionsIn non-HDM sensitized patients, shrimp sIgE ≥3.55 kUA/L and Der p 10 sIgE≥3.98 kUA/L give 100% sensitivity and specificity, respectively, to diagnose shrimp allergy. HDM sensitivity can influence sIgE levels to shrimp and shrimp/HDM components due to cross-reactivity.

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ASTHMA AND IMMUNOGLOBULIN E (IgE) ANTIBODIES AFTER RESPIRATORY SYNCYTIAL VIRUS (RSV) BRONCHIOLITIS: A PROSPECTIVE COHORT STUDY WITH MATCHED CONTROLS

PEDIATRICS ◽

10.1542/peds.98.2.329 ◽

1996 ◽

Vol 98 (2) ◽

pp. 329-329

Author(s):

James E. Gern

Keyword(s):

Risk Factor ◽

Cohort Study ◽

Respiratory Syncytial Virus ◽

Family History ◽

Prospective Cohort ◽

Immunoglobulin E ◽

Specific Ige ◽

Ige Antibodies ◽

History Of ◽

Syncytial Virus

RSV bronchiolitis was the most important risk factor for the development of asthma and allergen-specific IgE, although a family history of atopy or asthma further increased the risk.

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Proficiency Survey-Based Evaluation of Clinical Total and Allergen-Specific IgE Assay Performance

Archives of Pathology & Laboratory Medicine ◽

10.5858/2009-0518-oa.1 ◽

2010 ◽

Vol 134 (7) ◽

pp. 975-982 ◽

Cited By ~ 9

Author(s):

Robert G. Hamilton

Keyword(s):

Allergic Disease ◽

Immunoglobulin E ◽

Diagnostic Algorithm ◽

Specific Ige ◽

Total Serum ◽

Design Data ◽

Ige Antibody ◽

Marked Variability ◽

Specific Immunoglobulin E ◽

History Of

Abstract Context.—The diagnostic algorithm for human allergic disease involves confirmation of sensitization by detection of allergen-specific immunoglobulin E (IgE) antibody in individuals suspected of having allergic disease because of a history of allergic symptoms after known allergen exposure. Previous studies showed wide disparity among clinically reported allergen-specific IgE levels from different serologic assays. Objective.—To validate the relative analytic performance (sensitivity, interassay reproducibility, linearity/parallelism, intermethod agreement) of clinically used total and allergen-specific IgE assays by using College of American Pathologists' Diagnostic Allergy “SE” Proficiency Survey data. Design.—Data from 2 SE survey cycles were used to assess relative analytic performance of the ImmunoCAP (Phadia), Immulite (Siemens Healthcare-Diagnostics), and HYTEC 288 (HYCOR-Agilent Technologies) total and allergen-specific IgE assays. In each cycle, 2 recalcified plasma pools from atopic donors were diluted twice with IgE-negative serum and evaluated in approximately 200 federally certified clinical laboratories for total IgE and IgE antibody to 5 allergen specificities. Statistical analysis evaluated analytic sensitivity, linearity, reproducibility, and intermethod agreement. Results.—Interlaboratory intramethod, intermethod, and interdilution agreement of all 6 clinically used total serum IgE assays were excellent, with coefficients of variation (CVs) below 15%. Interlaboratory intramethod, and interdilution agreement of 3 clinically used allergen-specific IgE assays were also excellent with CVs below 15%. However, intermethod CVs identified between-assay disagreement greater than 20% in 80% of allergen-specific IgE measurements. Allergen reagents and patients' immune response heterogeneity are suggested probable causes. Conclusions.—Clinical total and allergen-specific IgE assays display excellent analytic sensitivity, precision, reproducibility, and linearity. Marked variability in quantitative estimates of allergen-specific IgE from clinically used automated immunoassays is a concern that may be ameliorated with component allergen use.

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Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach, Periplaneta fuliginosa

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.11.4.680-685.2004 ◽

2004 ◽

Vol 11 (4) ◽

pp. 680-685 ◽

Cited By ~ 12

Author(s):

Kyoung Yong Jeong ◽

Heeyu Hwang ◽

Jongweon Lee ◽

In-Yong Lee ◽

Dong Soo Kim ◽

...

Keyword(s):

Recombinant Protein ◽

Periplaneta Americana ◽

Immunoglobulin E ◽

Expression System ◽

Specific Ige ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Inhibitor Concentration ◽

The Cross ◽

Elisa Inhibition

ABSTRACTHousehold arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach,Periplaneta fuliginosa. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were performed withBlattella germanicaandDermatophagoides farinaeallergic sera. cDNA encoding tropomyosin, which is a well known cross-reactive pan-allergen, was cloned by reverse transcriptase PCR, and recombinant protein was produced by using a pET-28b expression system. Native tropomyosin was purified by ammonium sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities of native and recombinant tropomyosins were compared by an ELISA inhibition study. All 30 sera tested showedP. fuliginosa-specific IgE, and the IgE-binding reactivity of theP. fuliginosaextract was inhibited as much as 79.4% by aB. germanicaextract and as much as 63.3% by aD. farinaeextract. The deduced amino acid sequence of cloned cDNA was identical with that ofPeriplaneta americanatropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic sera had IgE specific for recombinant protein, and the maximum inhibition ofP. fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an inhibitor concentration of 10 μg/ml. Native tropomyosin inhibited the binding of IgE to theP. fuliginosa,B. germanica, andD. farinaeextracts by 65.0, 51.8, and 39% at an inhibitor concentration of 1 μg/ml.P. fuliginosaappears to possess allergens that are highly cross-reactive with allergens ofB. germanicaandD. farinae. Tropomyosin was found to be a major allergenic component accounting for the cross-reactivity between cockroaches and dust mites.

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The Dermatophagoides pteronyssinus Molecular Sensitization Profile of Allergic Rhinitis Patients in Central China

American Journal of Rhinology and Allergy ◽

10.1177/1945892418787116 ◽

2018 ◽

Vol 32 (5) ◽

pp. 397-403 ◽

Cited By ~ 2

Author(s):

Yongshi Yang ◽

Rongfei Zhu ◽

Nan Huang ◽

Wenjing Li ◽

Wei Zhang ◽

...

Keyword(s):

Allergic Rhinitis ◽

Immunoglobulin E ◽

Dermatophagoides Pteronyssinus ◽

Mite Species ◽

Cross Reactivity ◽

Central China ◽

House Dust Mites ◽

Positive Skin ◽

Der P2 ◽

Der P1

Background House dust mites (HDMs) are the major aeroallergens in patients with rhinitis and/or asthma in China. However, the molecular sensitization of HDM is varied in different regions. Objective To investigate the Dermatophagoides pteronyssinus ( Der p) molecular sensitization profile of allergic rhinitis (AR) patients in Central China. Methods AR patients with positive skin prick tests to Der p were enrolled in our study. Specific immunoglobulin E (sIgE) for Der p, Dermatophagoides farinae ( Der f), Acarus siro ( Aca s), Blomia tropicalis ( Blo t), and Der p components Der p1, Der p2, and Der p10 were measured in all patients. Results A total of 130 patients were included. The overall prevalence of positive serum sIgE was 99.2% for Der p, 98.5% for Der f, 81.5% for Aca s, 83.1% for Blo t, 71.5% for Der p1, 64.6% for Der p2, and 11.5% for Der p10. HDM-AR patients with asthma displayed significantly higher concentrations of sIgE to Der p, Der f, Der p1, and Der p2 than did those without asthma ( P < .001). The prevalence of asthma in HDM-AR patients was higher among the patients sensitized to both Der p1 and Der p2 (62.8%) than those sensitized to only 1 allergen ( Der p1 26.7% or Der p2 16.7%; P < .05) or nonsensitized to Der p1 and Der p2 (19.4%; P < .001). Conclusion Der p has high cross-reactivity with other mite species. Der p1 and Der p2 are the major components to induce Der p sensitization among AR patients in Central China. Sensitization to both Der p1 and Der p2 may be a risk factor for developing asthma in HDM-AR patients.

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Case Report: Allergic Reactivity to Mahaleb (Prunus mahaleb) Spice in a Subject With Almond and Other Tree Nut Allergies

Allergy & Rhinology ◽

10.1177/2152656720959083 ◽

2020 ◽

Vol 11 ◽

pp. 215265672095908

Author(s):

Lora Benoit ◽

Jongkit Masiri ◽

Harish Janagama ◽

Steven M. Gendel ◽

Mansour Samadpour

Keyword(s):

Skin Prick Testing ◽

Middle Eastern ◽

Specific Ige ◽

Cross Reactivity ◽

Enzyme Linked Immunosorbent Assay ◽

Tree Nut ◽

Polyclonal Igg ◽

History Of ◽

Specific Igg ◽

Food Recalls

Background Mahaleb is an aromatic spice prepared from the fruit stone of the St. Lucie Cherry that is used as a flavoring agent in traditional Turkish and Middle Eastern baking. Immunodiagnostic kits for almond, which are based on polyclonal almond-specific IgG antibodies, have been shown to demonstrate considerable cross-reactivity with mahaleb as was incidentally discovered during a cluster of allergen-related food recalls in 2015. Objective Though acute allergy to almond is somewhat common, allergies to mahaleb have not been previously documented. However, based on antigenic similarity observed with almond-specific IgG, it is predicted that mahaleb nut proteins would exhibit some level of cross-reactivity with almond-specific IgE and may therefore potentiate acute allergic symptoms in individuals with food allergy to almond. Case Presentation: Herein, we report on a 40-year old Caucasian female with longitudinal history of multiple tree nut allergies including allergy to almond, presenting with moderate pruritus and oropharyngeal swelling shortly following ingestion of mahaleb seed kernels. Methods and Results Skin-prick testing using extracts compounded from pistachio, almond, and mahaleb revealed positive wheals measuring 8, 3, and 7 mm respectfully. Indirect enzyme-linked immunosorbent assay (ELISA) using plate-bound antigens prepared from pistachio, almond, and mahaleb revealed IgG positive responses to all three targets. ELISA and Western blot analysis performed using goat anti-almond polyclonal IgG demonstrated significant cross-reactivity between almond and mahaleb, but not to pistachio. Conclusion This is the first documented case of acute allergy to mahaleb, co-occurring in the context of plural tree nut allergies, providing novel evidence that mahaleb may pose a risk to nut-allergic individuals and indicating a need for awareness of spice contamination with nut and mahaleb residues.

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Detection of Trichinella-specific IgE in human Trichinellosis: Creating a new test

Journal of the Serbian Chemical Society ◽

10.2298/jsc140402065d ◽

2014 ◽

Vol 79 (12) ◽

pp. 1477-1490 ◽

Cited By ~ 2

Author(s):

Marija Devic ◽

Alisa Gruden-Movsesijan ◽

Ljiljana Sofronic-Milosavljevic

Keyword(s):

Trichinella Spiralis ◽

Time Frame ◽

Specific Ige ◽

Elisa Test ◽

Cross Reactivity ◽

Clinical Criteria ◽

Negative Results ◽

Specificity And Sensitivity ◽

Test Specificity

Trichinellosis is a parasitic disease of humans caused by the nematode from the genus Trichinella, predominantly Trichinella spiralis (T. spiralis). If Trichinella infection is suspected, based on epidemiological link and clinical criteria within defined period of time, then finding of Trichinella-specific antibodies in the examined sera provides a definitive proof of the infection establishment. Detection of Trichinella-specific IgE that could precede, coincide or follow IgG seroconversion not only confirms the infection existence, but could narrow the time frame in which the infection took place to a year or even less. Since there are no commercially available tests for monitoring the Trichinella-specific IgE presence during the course of the disease, our work was aimed to establish this kind of ELISA test. Specificity and sensitivity of so far described Trichinella-specific IgE ELISAs are not satisfying enough; two major problems are poor discrimination between positive and negative results and cross reactivity with sera of patients with different parasitic diseases. In this study, we have developed Trichinella-specific IgE Capture ELISA that overcomes problems with specificity and sensitivity and enables determination of Trichinella-specific IgE.

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Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection

Parasitology ◽

10.1017/s0031182096008633 ◽

1997 ◽

Vol 114 (4) ◽

pp. 395-406 ◽

Cited By ~ 56

Author(s):

F. N. J. KOOYMAN ◽

P. J. S. VAN KOOTEN ◽

J. F. HUNTLEY ◽

A. MacKELLAR ◽

A. W. C. A. CORNELISSEN ◽

...

Keyword(s):

Monoclonal Antibody ◽

Haemonchus Contortus ◽

Immunoglobulin E ◽

Polyclonal Antibodies ◽

Sandwich Elisa ◽

Specific Ige ◽

Cross Reactivity ◽

Total Serum ◽

Western Blots ◽

Serum Ige

Part of the Cε3–Cε4 region of the ovine immunoglobulin E (IgE) gene (nucleotides 1111–1575) was amplified by PCR. The recombinant protein (recIgE1-2) was expressed in E. coli and both monoclonal and polyclonal antibodies were produced. These antibodies recognized recIgE1-2 and native IgE on Western blots and in ELISA. The polyclonal serum showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal antibody was specific for ovine IgE and goat IgE. Infection of sheep with the abomasal nematode Haemonchus contortus resulted in elevated IgE levels in serum 2–4 weeks after infection, as measured by sandwich ELISA using the rabbit polyclonal as capture antibody and the monoclonal antibody against ovine IgE as second antibody. A negative correlation between worm counts and total serum IgE levels at the end of the experiment was found in repeatedly infected sheep. Significant increased levels of excretory–secretory antigens specific IgE levels were found after H. contortus infection. In contrast, no significant changes in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected after infection.

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Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.10.2.229-232.2003 ◽

2003 ◽

Vol 10 (2) ◽

pp. 229-232 ◽

Cited By ~ 4

Author(s):

Yee-Hsuan Chiou ◽

Chung-Yee Yuo ◽

Lin-Yu Wang ◽

Shiao-ping Huang

Keyword(s):

Immunoglobulin E ◽

Competitive Inhibition ◽

Allergic Patient ◽

Specific Ige ◽

Cross Reactivity ◽

Allergen Avoidance ◽

Ige Binding ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

The Common

ABSTRACT The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.

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Skin prick test reactivity in atopic children and their number of siblings

Paediatrica Indonesiana ◽

10.14238/pi55.4.2015.189-93 ◽

2015 ◽

Vol 55 (4) ◽

pp. 189

Author(s):

Beatrix Siregar ◽

Lily Irsa ◽

Supriatmo Supriatmo ◽

Sjabaroeddin Loebis ◽

Rita Evalina

Keyword(s):

Skin Prick Test ◽

Specific Ige ◽

Cross Sectional Study ◽

Chi Square ◽

Cross Sectional ◽

Positive Skin ◽

Prick Test ◽

Group I ◽

History Of ◽

Skin Prick Tests

Background Some studies have shown that low birth order is a risk factor for developing atopy, although these results remain inconclusive. Those studies put forth the hygiene hypothesis, which states that early childhood infections in siblings may protect against atopy. Hence, an inverse relationship between family numbers and atopy was found. Atopy may be diagnosed from a history of atopy in an individual or his family, and can be confirmed by specific IgE for allergens or positive skin prick tests.Objective To assess for an association between skin prick test reactivity in atopic children and their number of siblings.Methods A cross-sectional study was conducted in May to June 2010 in elementary school children at the Kampung Baru District, Medan Regency, North Sumatera. Subjects were divided into two groups. Group I had children with < 3 siblings and group II had children with ≥ 3 siblings. Skin prick tests were done in 7 to 10-year-old children with a history of asthma, allergic rhinitis and atopic dermatitis. Skin prick test reactivity results were analyzed by Chi-square test.Results A total of 192 subjects were enrolled in this study, with 96 subjects in each group. Positive skin prick tests were significantly higher in subjects with <3 siblings than in those with >3 siblings (75% and 53.1%, respectively; P=0.003).Conclusion Atopic children with <3 siblings had more positive skin prick tests than children with >3 siblings.

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