Luteolin abates reproductive toxicity mediated by the oxido-inflammatory response in Doxorubicin-treated rats

The anti-neoplastic use of Doxorubicin (DOX) is hampered by several limitations, including reproductive toxicity. Luteolin (LUT)–a phytochemical-biological benefits include antioxidative and anti-inflammatory actions. Here we examined the protective effect of LUT against DOX-induced reproductive toxicity in an in vivo model—male albino Wistar rats—randomly assigned to five groups and treated as follows: Control (corn oil 2 mL/kg; per os), LUT (100 mg/kg; per os), DOX (2 mg/kg) by intraperitoneal injections, co-treated groups received LUT (50 and 100 mg/kg) with DOX. Treatment with DOX alone, significantly (p > 0.05), reduced biomarkers of testicular function, reproductive hormone levels, testicular and epididymal antioxidant, and anti-inflammatory cytokine. DOX increased (p > 0.05) sperm morphological abnormalities, as well as reactive oxygen and nitrogen species, lipid peroxidation, xanthine oxidase, a pro-inflammatory cytokine, and apoptotic biomarkers. Furthermore, testicular and epididymal histological lesion complemented the observed biochemical changes in treated rats. LUT co-treatment resulted in a dosage-dependent improvement in rats’ survivability, antioxidants capacity, reduction in biomarkers of oxidative stress, pro-inflammatory cytokines, and apoptosis in rat’s testis and epididymis. Also, LUT treatment resulted in improved histological features in the testis and epididymis, relative to DOX alone treated rats. LUT co-treatment abated DOX-mediated reproductive organ injuries associated with pro-oxidative, inflammatory, and apoptotic mechanisms. LUT supplementation may serve as a phyto-protective agent in alleviating male reproductive organ toxic injuries associated with Doxorubicin therapy.

Download Full-text

MDMA (“Ecstasy”) suppresses the innate IFN-γ response in vivo: A critical role for the anti-inflammatory cytokine IL-10

European Journal of Pharmacology ◽

10.1016/j.ejphar.2007.07.020 ◽

2007 ◽

Vol 572 (2-3) ◽

pp. 228-238 ◽

Cited By ~ 29

Author(s):

Noreen T. Boyle ◽

Thomas J. Connor

Keyword(s):

Inflammatory Cytokine ◽

Critical Role ◽

Ifn Γ ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine

Download Full-text

Migration, Distribution, and Safety Evaluation of Specific Phenotypic and Functional Mouse Spleen-Derived Invariant Natural Killer T2 Cells after Adoptive Infusion

Mediators of Inflammation ◽

10.1155/2021/5170123 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Dongzhi Chen ◽

Wenbin Xu ◽

Jingfang Teng ◽

Huifang Liu ◽

Yuanyuan Wang ◽

...

Keyword(s):

Natural Killer ◽

Cell Sorting ◽

Intraperitoneal Injection ◽

Inflammatory Cytokine ◽

Inkt Cells ◽

Anti Inflammatory ◽

Invariant Natural Killer ◽

Anti Inflammatory Cytokine ◽

Magnetic Activated Cell Sorting

Herein, the migration distribution and safety of specific phenotypic and functionally identified spleen-derived invariant natural killer T2 (iNKT2) cells after adoptive infusion in mice were studied. The proliferation and differentiation of iNKT cells were induced by intraperitoneal injection of α-galactosylceramide (α-GalCer) in vivo. Mouse spleens were isolated in a sterile environment. iNKT cells were isolated by magnetic-activated cell sorting columns (MS columns). Cytometric bead array (CBA) assay was used to detect cytokine secretion in the supernatant stimulated by iNKT cells. The basic life status of the mice was observed, and systematic quantitative scoring was conducted after injecting spleen-derived iNKT cells through the tail vein. An in vivo imaging system was used to trace the migration and distribution of iNKT cells in DBA mice. The percentage of the iNKT2 subgroup was the highest in 3 days after intraperitoneal injection of α-GalCer, and iNKT2 subsets accounted for more than 92% after separation and purification by magnetic-activated cell sorting (MACS). Anti-inflammatory cytokine IL-4 was mainly found in the supernatant of cell cultures. The adoptive infusion of iNKT cells into healthy mice resulted in no significant change in the basic life status of mice compared with the noninjected group. iNKT cells were detected in the lung, spleen, and liver, but no fluorescence was detected in lymph nodes and thymus. After dissecting the mice, it was found that there were no significant abnormalities in the relevant immune organs, brain, heart, kidney, lung, and other organs. Intraperitoneal injection of α-GalCer results in a large number of iNKT2 cells, mainly secreting anti-inflammatory cytokine IL-4, from the spleen of mice. After adoptive infusion, the iNKT2 cells mainly settled in the liver and spleen of mice with a satisfactory safety profile.

Download Full-text

Pro- and Anti-inflammatory Cytokine Content in Human Peripheral Blood after Its Transcutaneous (in Vivo) and Direct (in Vitro) Irradiation with Polychromatic Visible and Infrared Light

Photomedicine and Laser Surgery ◽

10.1089/pho.2006.24.129 ◽

2006 ◽

Vol 24 (2) ◽

pp. 129-139 ◽

Cited By ~ 38

Author(s):

Natalya A. Zhevago ◽

Kira A. Samoilova

Keyword(s):

Peripheral Blood ◽

Inflammatory Cytokine ◽

Human Peripheral Blood ◽

Infrared Light ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine

Download Full-text

Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo

Scientific Reports ◽

10.1038/s41598-017-05230-2 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 13

Author(s):

Byung Cheon Lee ◽

Sang-Goo Lee ◽

Min-Kyung Choo ◽

Ji Hyung Kim ◽

Hae Min Lee ◽

...

Keyword(s):

Gene Expression ◽

Immune Response ◽

Inflammatory Cytokine ◽

Cytokine Gene Expression ◽

Cytokine Gene ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine

Download Full-text

Biphasic Effect ofPhyllanthus emblicaL. Extract on NSAID-Induced Ulcer: An Antioxidative Trail Weaved with Immunomodulatory Effect

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/146808 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 16

Author(s):

Ananya Chatterjee ◽

Subrata Chattopadhyay ◽

Sandip K. Bandyopadhyay

Keyword(s):

Inflammatory Cytokine ◽

Ethanolic Extract ◽

Protein Carbonyl ◽

Protein Carbonyl Content ◽

Biphasic Effect ◽

Anti Inflammatory ◽

Anti Inflammatory Cytokine ◽

Antioxidative Property

Amla (Phyllanthus emblicaL.), apart from its food value, can be used as a gastroprotective agent in non steroidal anti-inflammatory drug (NSAID)-induced gastropathy. It has been suggested that the antioxidative property of amla is the key to its therapeutic effect. Hence, on the basis ofin vitroantioxidative potential, the ethanolic extract of amla (eAE) was selected forin vivostudy in NSAID-induced ulcer. Intriguingly, eAE showed biphasic activity in ulcerated mice, with healing effect observed at 60 mg/kg and an adverse effect at 120 mg/kg.The dose-dependent study revealed that switching from anti-oxidant to pro-oxidant shift and immunomodulatory property could be the major cause for its biphasic effect, as evident from the total antioxidant status, thiol concentration, lipid peroxidation, protein carbonyl content followed by mucin content, synthesis and cytokine status. Further, Buthionine sulfoxamine (BSO) pretreatment established the potential impact of antioxidative property in the healing action of eAE. However, eAE efficiently reduced pro-inflammatory cytokine (TNF- and IL-1) levels and appreciably upregulate anti-inflammatory cytokine (IL-10) concentration. In conclusion, gastric ulcer healing induced by eAE was driven in a dose-specific manner through the harmonization of the antioxidative property and modulation of anti-inflammatory cytokine level.

Download Full-text