Technical aspects of lymphoma immunohistology.

Immunohistological techniques (i.e., based on tissue sections) for the study of human lymphoma have been developed in recent years as alternatives to immunocytochemical methods (based on cell suspensions). These techniques not only allow the important architectural features of the lymphoma sample to be preserved, but are also more convenient to use in that tissues can be processed rapidly and studied at leisure. The relative advantages and disadvantages of frozen versus fixed embedded tissue, and of immunofluorescence versus immunoenzymatic methods are reviewed, and technical aspects of proteolytic digestion (as a technique for enhancing immunohistochemical labeling) are discussed. This review concludes with a consideration of the problems encountered in the interpretation of immunohistochemical labeling for lymphoma cell immunoglobulin in paraffin sections.

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Discovery of intracellular 2,8 dihydroxyadenine crystals in bovine lymphatic and hepatic cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012881x ◽

1987 ◽

Vol 45 ◽

pp. 906-907

Author(s):

W. E. Rigsby ◽

D. M. Hinton ◽

V. J. Hurst ◽

P. C. McCaskey

Keyword(s):

Polarized Light ◽

Paraffin Sections ◽

Tissue Samples ◽

Whole Cells ◽

Tissue Sections ◽

Hepatic Cells ◽

Slaughter House ◽

Mammalian Tissues ◽

Carbon Coated ◽

Cellular Components

Crystalline intracellular inclusions are rarely seen in mammalian tissues and are often difficult to positively identify. Lymph node and liver tissue samples were obtained from two cows which had been rejected at the slaughter house due to the abnormal appearance of these organs in the animals. The samples were fixed in formaldehyde and some of the fixed material was embedded in paraffin. Examination of the paraffin sections with polarized light microscopy revealed the presence of numerous crystals in both hepatic and lymph tissue sections. Tissue sections were then deparaffinized in xylene, mounted, carbon coated, and examined in a Phillips 505T SEM equipped with a Tracor Northern X-ray Energy Dispersive Spectroscopy (EDS) system. Crystals were obscured by cellular components and membranes so that EDS spectra were only obtainable from whole cells. Tissue samples which had been fixed but not paraffin-embedded were dehydrated, embedded in Spurrs plastic, and sectioned.

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Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines

Data in Brief ◽

10.1016/j.dib.2020.106668 ◽

2021 ◽

Vol 34 ◽

pp. 106668

Author(s):

Alberto Montalbano ◽

Cesare Sala ◽

Chiara Abrardo ◽

Nicoletta Murciano ◽

Farhad Jahanfar ◽

...

Keyword(s):

Potassium Channels ◽

Cell Lines ◽

Lymphoma Cell ◽

Outward Currents ◽

Human Lymphoma

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The Computer-Assisted Web Interview Method as Used in the National Study of ICT Use in Primary Healthcare in Poland – Reflections on a Case Study

Studies in Logic Grammar and Rhetoric ◽

10.1515/slgr-2015-0046 ◽

2015 ◽

Vol 43 (1) ◽

pp. 137-146 ◽

Cited By ~ 1

Author(s):

Paweł Sowa ◽

Bartosz Pędziński ◽

Michalina Krzyżak ◽

Dominik Maślach ◽

Sylwia Wójcik ◽

...

Keyword(s):

Primary Healthcare ◽

National Study ◽

Computer Assisted ◽

Web Surveys ◽

Technical Aspects ◽

Interview Method ◽

Advantages And Disadvantages ◽

Ict Use ◽

Aforementioned Study

Abstract The development and widespread use of ICT in society are reflected by the way research is designed and conducted. The Computer Assisted Web Interview method is becoming more attractive and is a frequently used method in health sciences. The National Study of ICT Use in Primary Healthcare in Poland was conducted using this method. The aim of this paper is to present the major advantages and disadvantages of web surveys. Technical aspects of methodology and important stages of the aforementioned study, as well as key elements for its procedure, are mentioned. The authors also provide reflections based on their analysis of this national study, conducted between January and April 2014.

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Induction of apoptosis in a human lymphoma cell line by hydrophobic peptide fraction separated from anchovy sauce

BioFactors ◽

10.1002/biof.552210112 ◽

2004 ◽

Vol 21 (1-4) ◽

pp. 63-67 ◽

Cited By ~ 35

Author(s):

Young Gon Lee ◽

Ki Won Lee ◽

Ji Yeon Kim ◽

Kyoung Heon Kim ◽

Hyong Joo Lee

Keyword(s):

Cell Line ◽

Lymphoma Cell ◽

Lymphoma Cell Line ◽

Peptide Fraction ◽

Hydrophobic Peptide ◽

Human Lymphoma ◽

Anchovy Sauce ◽

Induction Of Apoptosis

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A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA.

Journal of Histochemistry & Cytochemistry ◽

10.1177/41.1.7678025 ◽

1993 ◽

Vol 41 (1) ◽

pp. 7-12 ◽

Cited By ~ 542

Author(s):

J H Wijsman ◽

R R Jonker ◽

R Keijzer ◽

C J van de Velde ◽

C J Cornelisse ◽

...

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Staining Method ◽

Morphological Characteristics ◽

Image Cytometry ◽

Apoptotic Cells ◽

Dna Breaks ◽

Paraffin Sections ◽

Tissue Sections

Apoptosis (programmed cell death) can be difficult to detect in routine histological sections. Since extensive DNA fragmentation is an important characteristic of this process, visualization of DNA breaks could greatly facilitate the identification of apoptotic cells. We describe a new staining method for formalin-fixed, paraffin-embedded tissue sections that involves an in situ end-labeling (ISEL) procedure. After protease treatment to permeate the tissue sections, biotinylated nucleotides are in situ incorporated into DNA breaks by polymerase and subsequently stained with DAB via peroxidase-conjugated avidin. Staining of cells with the morphological characteristics of apoptosis was demonstrated in tissues known to exhibit programmed cell death, i.e., prostate and uterus after castration, tumors, lymph node follicles, and embryos. Apoptotic cells could be discriminated morphologically from areas of labeled necrotic cells, in which DNA degradation also occurs. Because apoptosis is relatively easily recognized in H&E-stained sections of involuting prostates of castrated rats, we used this model system to validate the ISEL method for the quantification of apoptotic cells. A high correlation was found between the fractions of ISEL-labeled cells and the fractions of apoptotic cells that were morphologically determined in adjacent sections. We conclude that ISEL is a useful technique for quantification of apoptosis in paraffin sections, especially for those tissues in which morphological determination is difficult. Furthermore, this new staining method enables the use of automated image cytometry for evaluating apoptosis.

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