Microphotometric analysis of protein-bound thiols and disulfides with an azogenic maleimide.
1981 ◽
Vol 29
(7)
◽
pp. 817-821
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Keyword(s):
Low Ph
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The chromogenic thiol reagent N-(4-aminophenyl)-maleimide (APM) was used to provide a sensitive procedure for the determination of protein-bound thiol group, both before and after reduction of disulfide bonds by tri-n-butylphosphine. The bound reagent is diazotized and coupled at low pH to N-(1-naphthyl)ethylenediamine to form an intense magenta label (lambda max 547 nm) with a high molar absorbancy (epsilon 547 = 49,800 cm2/mmol). The validity of absolute quantitation afforded by the method was shown by comparing the results with standard chemical analyses of thiol contents in a model system. Furthermore, the applicability of the procedure to biological objects was shown on isolated epididymal bull sperm heads.
1964 ◽
Vol 12
(01)
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pp. 119-125
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Keyword(s):
Keyword(s):
Keyword(s):
1968 ◽
Vol 33
(2)
◽
pp. 441-461
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Keyword(s):
1993 ◽
Vol 268
(12)
◽
pp. 8580-8589
2004 ◽
Vol 69
(4)
◽
pp. 273-282
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1996 ◽
Vol 15
(6)
◽
pp. 497-503
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