Phenotyping of Lipemias by Ultrafiltration and Nephelometry of Serum Lipoproteins
Keyword(s):
Abstract Nephelometry of serum before and after ultrafiltration through cellulose membranes affords a simple and rapid physical method for separately estimating pre-β-lipoproteins and chylomicrons in their native state. The method was rigorously evaluated through correlative studies with chemical analyses, electron microscopy, and electrophoresis. Together, the data indicate that this technique, combined with the determination of cholesterol as an estimate of β-lipoprotein concentration, allows the phenotyping of hyperlipemias.