Block-staining tissues with potassium ferrocyanide-reduced osmium tetroxide and lead aspartate for electron microscopic radioautography.
1984 ◽
Vol 32
(5)
◽
pp. 552-554
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Keyword(s):
En Bloc
◽
In the hope of devising a method for prestaining tissues en bloc for electron microscopic radioautography, pieces of radioiodine-labeled liver were taken through various combinations of ferrocyanide-reduced osmium tetroxide, lead aspartate, and aqueous uranyl acetate at room temperature or at 60 degrees C. Following the tests, the method adopted for routine use was to block-stain tissues for 2 hr in potassium ferrocyanide-reduced osmium tetroxide at 4 degrees C followed by 1 hr in Walton's lead aspartate at room temperature. This simple method, which requires no manipulation before or after emulsion coating and development of the radioautographs, provides adequate contrast without inducing background fog or artifacts.
1967 ◽
Vol 34
(3)
◽
pp. 757-771
◽
1971 ◽
Vol 29
◽
pp. 524-525
1988 ◽
Vol 46
◽
pp. 346-347
1968 ◽
Vol 26
◽
pp. 20-21
1994 ◽
Vol 52
◽
pp. 1018-1019
1994 ◽
Vol 52
◽
pp. 356-357
1990 ◽
Vol 48
(3)
◽
pp. 130-131