scholarly journals Expression of tenascin in developing and adult mouse lymphoid organs.

1993 ◽  
Vol 41 (8) ◽  
pp. 1163-1169 ◽  
Author(s):  
G Ocklind ◽  
J Talts ◽  
R Fässler ◽  
A Mattsson ◽  
P Ekblom
Keyword(s):  
Lymph Nodes ◽  
Northern Blot ◽  
Adult Mouse ◽  
Histological Analysis ◽  
Lymphoid Cells ◽  
Differential Splicing ◽  
Cell Functions ◽  
Mouse Tissues ◽  
Adult Thymus ◽  
Short Splice Variant

The extracellular matrix (ECM) is essential in regulating many cell functions in non-lymphoid cells, and the ECM may also play a role in the function of the immune system. Tenascin is a hexameric glycoprotein of the ECM. In mouse, two major polypeptides of MW 210 KD and 260 KD are formed by differential splicing. Northern blot screening of various mouse tissues showed that the short 6 KB tenascin message was strongly expressed in the adult thymus, whereas very little or no tenascin mRNA could be detected in spleen. In addition, immunoblotting and histological analysis with monoclonal anti-tenascin antibodies revealed the presence of tenascin in lymph nodes and spleen. In thymus, only a short-splice variant of tenascin was detected by immunoblotting, which supported the Northern blot results. Immunohistology showed that the epithelial reticular stroma in both embryonic and adult mouse thymus expressed tenascin, as did the postnatal mesenchymal reticular stroma in lymph nodes and spleen. The distribution of tenascin in the thymus was more restricted than that of fibronectin and laminin.

scholarly journals Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.

1985 ◽  
Vol 161 (3) ◽  
pp. 475-489 ◽  
Author(s):  
S H Lee ◽  
P M Starkey ◽  
S Gordon
Keyword(s):  
Bone Marrow ◽  
Small Bowel ◽  
Lymph Nodes ◽  
Large Bowel ◽  
Adult Mouse ◽  
Specific Antigen ◽  
Lymphoid Tissues ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Mouse Tissues

We have estimated the macrophage content of different tissues of the normal adult mouse using F4/80, a highly specific antigen marker for mature mouse macrophages. An absorption indirect binding assay was used to quantitate F4/80 antigen against a calibration standard made from the J774.2 macrophage-like cell line. The richest sources of tissue F4/80 antigen were found to be bone marrow, spleen, cervical and mesenteric lymph nodes, large bowel, liver, kidneys, and small bowel. The organs that have the highest total F4/80 antigen content are the liver, large bowel, small bowel, bone marrow, spleen, cervical and mesenteric lymph nodes, and kidney. We conclude that the mononuclear phagocyte system is mainly distributed in the gastrointestinal tract and liver, followed by hemopoietic and lymphoid tissues.

scholarly journals SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION

1974 ◽  
Vol 139 (6) ◽  
pp. 1488-1498 ◽  
Author(s):  
Wolfgang Tittor ◽  
Maria Gerbase-Delima ◽  
Roy L. Walford
Keyword(s):  
Young Adult ◽  
Lymph Nodes ◽  
Mixed Lymphocyte Reaction ◽  
Lymphoid Cells ◽  
The Other ◽  
Host Reaction ◽  
Migratory Patterns ◽  
Cell Subpopulations ◽  
The One ◽  
Adult Thymus

The present studies have shown that two subpopulations of thymus-dependent lymphocytes may act synergistically in the mixed lymphocyte reaction (MLR) in the mouse. One subpopulation was well represented in the young adult thymus and the other in lymph nodes. For optimum synergy, both populations must be allogeneic to the stimulator cells. Pretreatment of either population with mitomycin-C abolished synergy. Anti-θ serum abolished both MLR responding and synergizing activities of lymphoid cells. The two thymus-dependent subpopulations were both present in the spleen, and displayed different migratory patterns when injected into irradiated mice: one population went to spleens of the irradiated mice, the other to lymph nodes. The effects of anti-thymocyte serum on the MLR and upon synergy were assessed. While minor differences exist and are herein described, our overall results strongly suggest that in our experiments with synergy in MLR, we may be dealing with the same T1- and T2-cell subpopulations described by Cantor and Asofsky and coworkers (1, 2, 4, 5, 14) as displaying synergy in the graft-vs.-host reaction.

Interrelationships of myeloid and lymphoid cells: studies with chromosome-marked cells transfused into lethally irradiated mice

1966 ◽  
Vol 165 (998) ◽  
pp. 78-102 ◽  
Keyword(s):  
Bone Marrow ◽  
Lymph Nodes ◽  
Donor Cell ◽  
Cell Suspensions ◽  
Lymphoid Cells ◽  
Thoracic Duct Lymph ◽  
Dividing Cells ◽  
Bone Marrow Derived Cells ◽  
One Year ◽  
Adult Thymus

Lethally irradiated CBA mice were injected with a mixture of two cell suspensions, syngeneic with each other and with the host, but distinguished by the presence of either one or two T6 marker chromosomes. One of the cell suspensions was derived from adult bone marrow (10 5 cells), the other from adult thymus or pooled lymph nodes or thoracic duct lymph (10 7 cells). The recipients were killed between one day and one year after irradiation, having been injected 1 ½ h previously with Colcemid. Their bone marrow, spleen, thymus and lymph nodes were studied cytologically, and counts were made of the numbers of mitotic cells derived from the two donor cell suspensions and from the irradiated host. Bone marrow, spleen and thymus were all recolonized predominantly or exclusively by descendants of injected bone marrow cells. Descendants of injected lymphoid cells were seen in substantial numbers only in the lymph nodes, where they formed the majority of the total dividing cells between 1 and 3 weeks after irradiation. After that the proportion of such cells in the lymph nodes decreased gradually, in favour of bone marrow-derived cells, but they did not disappear completely. Lymphoid cells were equally unsuccessful at recolonizing the myeloid and thymic tissues of mice given a high sublethal dose of irradiation (800 rad) without bone marrow therapy. The normality of the repopulated lymph nodes and thymus was verified histologically and by two functional tests involving the capacity of cells rapidly to recolonize the lymph nodes or form macroscopic haematopoietic nodules in the spleen of further lethally irradiated mice. A small and decreasing amount of haematopoiesis was found in the lymph nodes during the first 2 months after irradiation, but not in the thymus.

scholarly journals HISTOLOGICAL ANALYSIS OF LUNGS AND PULMONARY LYMPH NODES IN CALVES WITH RESPIRATORY DISEASE

2021 ◽  
Vol 246 (2) ◽  
pp. 35-42
Author(s):  
А.К. Galiullin ◽  
◽  
I.N. Zalyalov ◽  
V.G. Gumerov ◽  
A. Gueriche ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Lymphoid Tissue ◽  
Histological Analysis ◽  
Clinical Signs ◽  
Lymphoid Cells ◽  
Heart Cells ◽  
Short Course ◽  
Distorted Structure ◽  
Pulmonary Pathology ◽  
Tissue Cells

In order to identify pathomorphological changes, histological studies of the lungs of calves from two groups were performed. Calves of the first group had parainfluenza, and the second group included calves with an acute course of catarrhal purulent bronchopneumonia with clinical signs of chronic and acute pulmonary pathology. Clinical and histological methods of investigation were used in the work. Established pathomorphological changes in the lungs of calves of the first group in the form of chronic interstitial bronchopneumonia accompanied by compensatory chronic alveo-lar emphysema, formation of multinucleated symplast and multicellular syncytiae from the epitheli-um of alveoli, as well as mixed cytopathic forms of respiratory epithelium - symplasts-syncytiae are characteristic cytological signs of chronic parainfluenza infection. Detection of local thickening of bronchial walls due to proliferation of multilobular epithelium and infiltration of its submucosal base by lymphoid cells with narrowed lumen profiles also confirms parainfluenza etiology of the changes that occurred in the lungs. Marked manifestations of bronchopneumonia were manifested with maximum intensity in ventilated cardiac and diaphragmatic lungs of sick calves. Chronic inflammatory process in the lungs at parainfluenza was complicated by fibrinous pleurisy, as well as peri- and endocarditis, myocardiodystrophy with distorted structure of cardio-myocytes and atypical heart cells. In the pulmonary lymph nodes of parainfluenza-affected calves a moderately pronounced hyperplasia of lymphoid tissue cells with the presence of secondary lymph nodes, some of which had a pronounced structure of dark cellular periphery and a broad cell-rich germinative zone. In calves of the second group with an acute course of catarrhal purulent broncho-pneumonia we could not detect the above cytopathic changes in the airways and the respiratory part of the lungs. Pathological changes in the lungs were predominantly exudative, represented by se-rous alveolitis, catarrhal purulent bronchopneumonia with involvement of anterior and cardiac lobes of the organ. Short course of the disease was accompanied by weak proliferation of lymphoid tissue cells in pulmonary lymph nodes.

scholarly journals The mouse vitronectin receptor is a T cell activation antigen.

1991 ◽  
Vol 173 (2) ◽  
pp. 343-347 ◽  
Author(s):  
K Moulder ◽  
K Roberts ◽  
E M Shevach ◽  
J E Coligan
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Lymphoid Cells ◽  
Vitronectin Receptor ◽  
Cell Functions ◽  
Murine Homologue ◽  
Accessory Molecule ◽  
T Cell Lines ◽  
Activation Antigen

In this report, we demonstrate that the T cell activation antigen, recognized by monoclonal antibody H9.2B8, is the murine homologue of the vitronectin receptor (VNR) and, thereby, we provide initial evidence that VNR is expressed on lymphoid cells. VNR is expressed on a variety of T cell lines, tumors, and Con A-activated splenocytes, but not resting T cells, and is capable of binding to the extracellular matrix proteins fibronectin, fibrinogen, and vitronectin, via the tripeptide sequence RGD. There was no evidence of novel beta chains pairing with the VNR alpha chain, as has been demonstrated in some human cells. In view of recent studies demonstrating that this molecule functions as an accessory molecule in T cell activation, the VNR may play an important role in mouse T cell functions.

scholarly journals CELL-MEDIATED CYTOTOXICITY DURING REJECTION AND ENHANCEMENT OF ALLOGENEIC SKIN GRAFTS IN RATS

1972 ◽  
Vol 135 (6) ◽  
pp. 1301-1315 ◽  
Author(s):  
Hans-Hartmut Peter ◽  
Joseph D. Feldman
Keyword(s):  
Lymph Nodes ◽  
Thoracic Duct ◽  
Lymphoid Cells ◽  
Target Cells ◽  
Skin Grafts ◽  
Peak Activity ◽  
Adult Rats ◽  
Background Levels

Cell-mediated cytotoxicity (CMC) in spleens and lymph nodes of allografted rats was determined by release of 51Cr from labeled target cells incubated with aggressor lymphoid cells. CMC was first detected in grafted adult rats on day 5, peaked on days 7 and 8, and declined rapidly to background levels by days 9 to 11. In allografted neonates and in cyclophosphamide-treated or neonatally thymectomized adults CMC was a fraction of that observed in normal adult rats. Enhancing antibodies deferred in vivo peak activity of CMC in allografted neonates for 3–4 days, and blocked in vitro the action of aggressor lymphocytes by binding to target cells. Enhancing antibodies had no effect on the cytotoxicity of aggressor cells, but horse antibodies to rat thoracic duct cells inhibited in vitro CMC of aggressor cells.

scholarly journals 220- and 130-kDa MLCKs have distinct tissue distributions and intracellular localization patterns

2002 ◽  
Vol 282 (3) ◽  
pp. C451-C460 ◽  
Author(s):  
Emily K. Blue ◽  
Zoe M. Goeckeler ◽  
Yijun Jin ◽  
Ling Hou ◽  
Shelley A. Dixon ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Myosin Light Chain ◽  
Adult Mouse ◽  
Intracellular Localization ◽  
Myosin Ii ◽  
Nonmuscle Myosin ◽  
Functional Roles ◽  
Nonmuscle Myosin Ii ◽  
Mouse Tissues

To better understand the distinct functional roles of the 220- and 130-kDa forms of myosin light chain kinase (MLCK), expression and intracellular localization were determined during development and in adult mouse tissues. Northern blot, Western blot, and histochemical studies show that the 220-kDa MLCK is widely expressed during development as well as in several adult smooth muscle and nonmuscle tissues. The 130-kDa MLCK is highly expressed in all adult tissues examined and is also detectable during embryonic development. Colocalization studies examining the distribution of 130- and 220-kDa mouse MLCKs revealed that the 130-kDa MLCK colocalizes with nonmuscle myosin IIA but not with myosin IIB or F-actin. In contrast, the 220-kDa MLCK did not colocalize with either nonmuscle myosin II isoform but instead colocalizes with thick interconnected bundles of F-actin. These results suggest that in vivo, the physiological functions of the 220- and 130-kDa MLCKs are likely to be regulated by their intracellular trafficking and distribution.

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