Reticulocyte-secreted exosomes bind natural IgM antibodies: involvement of a ROS-activatable endosomal phospholipase iPLA2

Blood ◽  
2007 ◽  
Vol 110 (9) ◽  
pp. 3407-3416 ◽  
Author(s):  
Lionel Blanc ◽  
Céline Barres ◽  
Pascale Bette-Bobillo ◽  
Michel Vidal
Keyword(s):  
Reactive Oxygen Species ◽  
In Vitro Maturation ◽  
Membrane Vesicles ◽  
Apoptotic Cells ◽  
Red Cell ◽  
Oxygen Species ◽  
Igm Antibodies ◽  
Natural Igm ◽  
Rat Reticulocytes

Abstract Reticulocytes release small membrane vesicles termed exosomes during their maturation into erythrocytes. It has been suggested that reticulocytes remodel the plasma membrane of the immature red cell during erythropoiesis by specifically eliminating various proteins. We report here that exosome release is associated with a physiologic cascade induced by the expression of a 15-lipoxygenase at the reticulocyte stage. We found that the phospholipase iPLA2 specifically associated with the endosomal and exosomal membranes could be activated by reactive oxygen species (ROSs) produced during mitochondria degeneration induced by 15-lipoxygenase. Since iPLA2 has recently been demonstrated to participate in the clearance of apoptotic cells, we investigated its role in vesicle removal. We found that exosomes isolated directly from the blood of an anemic rat or released during in vitro maturation of rat reticulocytes bind IgM antibodies on their surface, in contrast to immature and mature red cells. These natural IgM antibodies recognize lysophosphatidylcholine and are able to specifically bind to apoptotic cells. Finally, evidence of C3 deposition on the exosome surface leads us to hypothesize that this cascade may favor the clearance of exosomes by cells once released into the bloodstream, via a mechanism similar to that involved in the elimination of apoptotic cells.

scholarly journals Vitamin E but Not GSH Decreases Reactive Oxygen Species Accumulation and Enhances Sperm Production during In Vitro Maturation of Frozen-Thawed Prepubertal Mouse Testicular Tissue

2019 ◽  
Vol 20 (21) ◽  
pp. 5380 ◽  
Author(s):  
Brahim Arkoun ◽  
Ludovic Galas ◽  
Ludovic Dumont ◽  
Aurélie Rives ◽  
Justine Saulnier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Vitamin E ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Testicular Tissue ◽  
Oxygen Species ◽  
Sperm Production ◽  
Ros Accumulation

Freezing–thawing procedures and in vitro culture conditions are considered as a source of stress associated with increased reactive oxygen species (ROS) generation, leading to a damaged cell aerobic metabolism and consequently to oxidative stress. In the present study, we sought to investigate whether vitamin E (Vit E) or reduced glutathione (GSH) enhances sperm production by decreasing ROS accumulation during in vitro maturation of prepubertal mice testes. Testes of prepubertal mice were cryopreserved using a freezing medium supplemented or not supplemented with Vit E and were cultured after thawing. In presence of Rol alone in culture medium, frozen-thawed (F-T) testicular tissues exhibited a higher ROS accumulation than fresh tissue during in vitro culture. However, Vit E supplementation in freezing, thawing, and culture media significantly decreased cytoplasmic ROS accumulation in F-T testicular tissue during in vitro maturation when compared with F-T testicular tissue cultured in the presence of Rol alone, whereas GSH supplementation in culture medium significantly increased ROS accumulation associated with cytolysis and tissue disintegration. Vit E but not GSH promoted a better in vitro sperm production and was a suitable ROS scavenger and effective molecule to improve the yield of in vitro spermatogenesis from F-T prepubertal mice testes. The prevention of oxidative stress in the cytoplasmic compartment should be regarded as a potential strategy for improving testicular tissue viability and functionality during the freeze–thaw procedure and in vitro maturation.

scholarly journals Suplementasi Antioksidan pada Maturasi In Vitro Menurunkan Kadar ROS: A Literature Review

2019 ◽  
Vol 11 (1) ◽  
pp. 23
Author(s):  
Amalia Ratna Kusumaningrum
Keyword(s):  
Reactive Oxygen Species ◽  
Literature Review ◽  
Assisted Reproductive Technology ◽  
In Vitro Maturation ◽  
Reactive Oxygen ◽  
Reproductive Technology ◽  
Fertility Treatment ◽  
Oxygen Species ◽  
Survival And Development

One of the reproductive health problems is infertility. In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment. IVM conditions inducing a series of events related to reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic development. Therefore, the supplementation of IVM media with antioxidants could improve the survival and development of the oocytes. The method of searching conducted online database such as Science Direct, Sage, Pubmed, Springer, and Oxford. The keywords that used were “antioxidant”, “reactive oxygen species”, “in vitro maturation”, “infertility” and “assisted reproductive technology”. The literatures restricted in 2008 year until 2019 in English. The results of the study show that there were 3 main themes, types, dosages, antioxidant benefits for reduced ROS levels during IVM. The drawback that might arise in this study is that there are still possibilities of not getting some related articles. The literature review is expected to be a basis for research assisted reproductive technology. Keywords: antioxidant; in vitro maturation; infertility; Reactive oxygen species (ROS)

scholarly journals Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olfa Chiboub ◽  
Ines Sifaoui ◽  
Manef Abderrabba ◽  
Mondher Mejri ◽  
José J. Fernández ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Mitochondrial Membrane ◽  
Brown Seaweed ◽  
Cell Permeability ◽  
Oxygen Species ◽  
Potential Drug ◽  
Concentration Cell ◽  
Drug Candidates

Abstract Background The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells. Methods The aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells. Results The results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death. Conclusions These results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi. Graphic abstract

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