scholarly journals L-asparaginase: acute effects on protein synthesis in rabbits with normal and increased fibrinogen production

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 823-827 ◽  
Author(s):  
BM Alving ◽  
CF Barr ◽  
DB Tang
Keyword(s):  
Protein Synthesis ◽  
New Zealand ◽  
Amino Acid ◽  
Plasma Proteins ◽  
Serum Proteins ◽  
Intravenous Dose ◽  
Single Intravenous Dose ◽  
Acute Effects ◽  
Abnormal Rate ◽  
New Zealand Rabbits

Abstract The acute effects of a single intravenous dose of L-asparaginase on protein synthesis were studied in normal rabbits and in animals that had received turpentine to stimulate fibrinogen production. Male New Zealand rabbits received L-asparaginase (500 U/kg) 16 hr before the injection of the radiolabeled amino acid [75Se]selenomethionine (75SeM). Incorporation of 75SeM into fibrinogen and serum proteins in the L-asparaginase-treated rabbits was the same as for saline-treated controls, with fibrinogen representing approximately 5% of the labeled plasma proteins. In turpentine-treated rabbits, the maximal incorporation of 75SeM into serum proteins remained unchanged, whereas 75SeM-fibrinogen increased sixfold and accounted for 25% of the labeled proteins. Animals that received L-asparaginase at the same time as turpentine or 14 hr later showed significant decreases in synthesis of both serum proteins and fibrinogen. 75SeM-fibrinogen that was purified from L-asparaginase-treated rabbits underwent normal catabolism when injected into normal recipient rabbits. These data indicate that L- asparaginase can acutely cause partial inhibition of both serum protein and fibrinogen synthesis when administered to rabbits shortly before or during a period of increased fibrinogen production. Fibrinogen that is synthesized in the presence of L-asparaginase does not have an abnormal rate of catabolism.

scholarly journals L-asparaginase: acute effects on protein synthesis in rabbits with normal and increased fibrinogen production

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 823-827
Author(s):  
BM Alving ◽  
CF Barr ◽  
DB Tang
Keyword(s):  
Protein Synthesis ◽  
New Zealand ◽  
Amino Acid ◽  
Plasma Proteins ◽  
Serum Proteins ◽  
Intravenous Dose ◽  
Single Intravenous Dose ◽  
Acute Effects ◽  
Abnormal Rate ◽  
New Zealand Rabbits

The acute effects of a single intravenous dose of L-asparaginase on protein synthesis were studied in normal rabbits and in animals that had received turpentine to stimulate fibrinogen production. Male New Zealand rabbits received L-asparaginase (500 U/kg) 16 hr before the injection of the radiolabeled amino acid [75Se]selenomethionine (75SeM). Incorporation of 75SeM into fibrinogen and serum proteins in the L-asparaginase-treated rabbits was the same as for saline-treated controls, with fibrinogen representing approximately 5% of the labeled plasma proteins. In turpentine-treated rabbits, the maximal incorporation of 75SeM into serum proteins remained unchanged, whereas 75SeM-fibrinogen increased sixfold and accounted for 25% of the labeled proteins. Animals that received L-asparaginase at the same time as turpentine or 14 hr later showed significant decreases in synthesis of both serum proteins and fibrinogen. 75SeM-fibrinogen that was purified from L-asparaginase-treated rabbits underwent normal catabolism when injected into normal recipient rabbits. These data indicate that L- asparaginase can acutely cause partial inhibition of both serum protein and fibrinogen synthesis when administered to rabbits shortly before or during a period of increased fibrinogen production. Fibrinogen that is synthesized in the presence of L-asparaginase does not have an abnormal rate of catabolism.

Platelet and Fibrinogen Production: Effect of Lipid a and Liposomes

1979 ◽  
Author(s):  
R.B. Ramsey ◽  
B.L. Evatt ◽  
B.M. Alving ◽  
C.R. Alving ◽  
M.B. Hamner
Keyword(s):  
Lipid A ◽  
Intravenous Dose ◽  
The Other ◽  
Response Relationship ◽  
Single Intravenous Dose ◽  
Dose Response Relationship ◽  
Platelet Production ◽  
E Coli ◽  
Lipid Moiety ◽  
New Zealand Rabbits

Previous studies have demonstrated that endotoxin administered in a single intravenous dose produced increased platelet and fibrinogen production. To determine if the lipid moiety of the endotoxin was implicated, we studied the effect of intact endotoxin (E. coli 026:B6), lipid A, lipid A incorporated into liposomes, and lipid A solubilized in triethylamine (TEA) on platelet and fibrinogen production in male New Zealand rabbits. Animals received these preparations by single intravenous 1 h infusions of 1.0, 5.0, 10.0, 25.0, or 50.0 μg/kg body weight. Selenomethionine-75Se was injected 18 h after infusion, and the percentages of incorporation into platelet and fibrinogen were used to measure thrombopoiesis and fibrinogen synthesis. All 4 types of infusions increased both platelet production and fibrinogen synthesis and a dose-response relationship was observed; however, the threshold dose for stimulation varied with the type of material infused. More lipid A (by weight) was required to stimulate either platelets or fibrinogen than any of the other materials infused. When incorporated into liposomes or solubilized with TEA, lipid A produced a response similar to that produced by intact endotoxin. These data suggest that the lipid A moiety itself can stimulate platelet and fibrinogen production.

Effects of training and competitive swimming on serum proteins

1961 ◽  
Vol 16 (5) ◽  
pp. 807-809 ◽  
Author(s):  
Thomas F. Johnson ◽  
Harry Y. C. Wong
Keyword(s):  
Plasma Protein ◽  
Plasma Proteins ◽  
Serum Proteins ◽  
College Men ◽  
Paper Electrophoresis ◽  
Acute Effects ◽  
Competitive Swimming ◽  
Protein Levels ◽  
Significant Difference ◽  
Beta Globulin

The major concern of this investigation was to observe chronic and/or acute effects of a typical intercollegiate training and competitive swimming program on plasma protein levels in young men. Plasma protein levels, followed over a period of 14 months in seven young college men, were analyzed by the technique of paper electrophoresis. No chronic effects in plasma proteins were observed; that is, no permanent change in plasma protein level was seen. Comparisons of data between competitive and noncompetitive seasons showed no significant difference. However, there were acute effects resulting from brief maximal exercise efforts. Albumin increased in response to participation in one or more competitive swimming events. A slight decrease was observed in the beta-globulin fraction. A similar albumin-beta pattern was seen in another group of varsity swimmers sprinting 220 yards. In both instances, the greatest deviation from pre-exercise levels was observed the next morning, some 11—20 hr later. Some of the mechanisms thought to act in causing a temporary alteration in plasma proteins during and after exercise are discussed. Submitted on October 12, 1960

The Acute Effects of Growth Hormone on Amino Acid Transport and Protein Synthesis Are Due to Its Insulin-Like Action*

Endocrinology ◽  
1988 ◽  
Vol 122 (2) ◽  
pp. 471-474 ◽  
Author(s):  
C.M. CAMERON ◽  
J.L. KOSTYO ◽  
N.A. ADAMAFIO ◽  
P. BROSTEDT ◽  
P. ROOS ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Acute Effects

Cytopathology of Cardiac Tissue from Daunomycin-Treated Mice

1971 ◽  
Vol 29 ◽  
pp. 550-551
Author(s):  
Robert H. Liss ◽  
Frances A. Cotton
Keyword(s):  
Cardiac Tissue ◽  
Cardiac Toxicity ◽  
Drug Distribution ◽  
Personal Communication ◽  
Intravenous Dose ◽  
Single Intravenous Dose ◽  
Physiologic Saline ◽  
Histopathologic Changes ◽  
Distribution Studies ◽  
Lung And Kidney

Daunomycin, an antibiotic used in the clinical management of acute leukemia, produces a delayed, lethal cardiac toxicity. The lethality is dose and schedule dependent; histopathologic changes induced by the drug have been described in heart, lung, and kidney from hamsters in both single and multiple dose studies. Mice given a single intravenous dose of daunomycin (10 mg/kg) die 6-7 days later. Drug distribution studies indicate that the rodents excrete most of a single dose of the drug as daunomycin and metabolite within 48 hours after dosage (M. A. Asbell, personal communication).Myocardium from the ventricles of 6 moribund BDF1 mice which had received a single intravenous dose of daunomycin (10 mg/kg), and from controls dosed with physiologic saline, was fixed in glutaraldehyde and prepared for electron microscopy.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

Sign in / Sign up

Export Citation Format

Share Document