scholarly journals Effect of leukocyte antibodies and HLA matching on the intravascular recovery, survival, and tissue localization of 111-indium granulocytes

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 522-528 ◽  
Author(s):  
J McCullough ◽  
M Clay ◽  
D Hurd ◽  
K Richards ◽  
C Ludvigsen ◽  
...  
Keyword(s):  
Hla Antigens ◽  
Pulmonary Sequestration ◽  
Strong Relationship ◽  
Immunofluorescence Assay ◽  
Hla Matching ◽  
Hla Antigen ◽  
Tissue Localization ◽  
The Relationship

The effect of leukocyte antibodies detected under different conditions on the fate in vivo of granulocytes was studied using 111-indium- labeled granulocytes. Sera from patients were tested by granulocyte agglutination (GA), granulocytotoxicity (GC), granulocyte immunofluorescence (GIF), lymphocytotoxicity (LC), and antibody- dependent lymphocyte-mediated granulocytotoxicity. Granulocytes from donors to be studied were labeled with 111-indium and injected. Then the intravascular recovery and survival or tissue localization was determined in 93 studies. Antibodies detected by granulocyte agglutination were associated with a significant reduction in recovery (6.7% v 30.8% in controls; P less than .001) and t1/2 (0.3 hours v 5.6 hours in controls; P = .002). When all possible combinations of serum reactivity were considered, reactivity in the GA plus GIF assays had the best correlation with decreased recovery (R2 = .49; P less than .001) and t1/2 (R2 = .73; P less than .001). When the relationship between the strength of antibody reactivity and the recovery and t1/2 were analyzed, the best relationship was between the combination of LC and GIF with recovery (R2 = .62; P = .001). Because of the general availability of the HLA (LC) testing, the role of LC reactivity was investigated in other ways. There was a strong relationship between sera highly reactive by LC and those reactive by GIF. These highly reactive sera were also associated with reduced recovery and t1/2. The influence of specific HLA antigen mismatches was also studied. When donor and recipient were mismatched for the HLA-A2, B8, or BW44 antigens, there was a significant reduction in either recovery, t1/2, or both. Tissue localization was studied by body scans in patients with and without known sites of inflammation. Antibodies detected by a combination of GA and GIF caused abnormal pulmonary sequestration of granulocytes (three cases) and failure of granulocytes to localize at known sites of inflammation (three cases). HLA (LC) antibodies did not alter tissue localization despite the presence of the corresponding HLA antigens on granulocytes. It appears that GA, GIF, or a combination of these tests is the most effective predictor of altered in vivo fate of granulocytes. However, sera highly reactive by LC and GIF probably define a group of highly immunized patients in whom granulocyte recovery and t1/2 are also reduced. Mismatching for certain HLA antigens is also associated with reduced granulocyte recovery and survival. At present, GA, with or without the immunofluorescence assay, is the most effective predictor of altered in vivo granulocyte activity.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Effect of leukocyte antibodies and HLA matching on the intravascular recovery, survival, and tissue localization of 111-indium granulocytes

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 522-528 ◽  
Author(s):  
J McCullough ◽  
M Clay ◽  
D Hurd ◽  
K Richards ◽  
C Ludvigsen ◽  
...  
Keyword(s):  
Hla Antigens ◽  
Pulmonary Sequestration ◽  
Strong Relationship ◽  
Immunofluorescence Assay ◽  
Hla Matching ◽  
Hla Antigen ◽  
Tissue Localization ◽  
The Relationship

Abstract The effect of leukocyte antibodies detected under different conditions on the fate in vivo of granulocytes was studied using 111-indium- labeled granulocytes. Sera from patients were tested by granulocyte agglutination (GA), granulocytotoxicity (GC), granulocyte immunofluorescence (GIF), lymphocytotoxicity (LC), and antibody- dependent lymphocyte-mediated granulocytotoxicity. Granulocytes from donors to be studied were labeled with 111-indium and injected. Then the intravascular recovery and survival or tissue localization was determined in 93 studies. Antibodies detected by granulocyte agglutination were associated with a significant reduction in recovery (6.7% v 30.8% in controls; P less than .001) and t1/2 (0.3 hours v 5.6 hours in controls; P = .002). When all possible combinations of serum reactivity were considered, reactivity in the GA plus GIF assays had the best correlation with decreased recovery (R2 = .49; P less than .001) and t1/2 (R2 = .73; P less than .001). When the relationship between the strength of antibody reactivity and the recovery and t1/2 were analyzed, the best relationship was between the combination of LC and GIF with recovery (R2 = .62; P = .001). Because of the general availability of the HLA (LC) testing, the role of LC reactivity was investigated in other ways. There was a strong relationship between sera highly reactive by LC and those reactive by GIF. These highly reactive sera were also associated with reduced recovery and t1/2. The influence of specific HLA antigen mismatches was also studied. When donor and recipient were mismatched for the HLA-A2, B8, or BW44 antigens, there was a significant reduction in either recovery, t1/2, or both. Tissue localization was studied by body scans in patients with and without known sites of inflammation. Antibodies detected by a combination of GA and GIF caused abnormal pulmonary sequestration of granulocytes (three cases) and failure of granulocytes to localize at known sites of inflammation (three cases). HLA (LC) antibodies did not alter tissue localization despite the presence of the corresponding HLA antigens on granulocytes. It appears that GA, GIF, or a combination of these tests is the most effective predictor of altered in vivo fate of granulocytes. However, sera highly reactive by LC and GIF probably define a group of highly immunized patients in whom granulocyte recovery and t1/2 are also reduced. Mismatching for certain HLA antigens is also associated with reduced granulocyte recovery and survival. At present, GA, with or without the immunofluorescence assay, is the most effective predictor of altered in vivo granulocyte activity.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Role of interleukins in the pathogenesis of pulmonary fibrosis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yi Xin She ◽  
Qing Yang Yu ◽  
Xiao Xiao Tang
Keyword(s):  
Pulmonary Fibrosis ◽  
Therapeutic Strategy ◽  
Future Directions ◽  
Regulation Mechanisms ◽  
Underlying Mechanisms ◽  
Multiple Cells ◽  
The Relationship

AbstractInterleukins, a group of cytokines participating in inflammation and immune response, are proved to be involved in the formation and development of pulmonary fibrosis. In this article, we reviewed the relationship between interleukins and pulmonary fibrosis from the clinical, animal, as well as cellular levels, and discussed the underlying mechanisms in vivo and in vitro. Despite the effects of interleukin-targeted treatment on experimental pulmonary fibrosis, clinical applications are lacking and unsatisfactory. We conclude that intervening in one type of interleukins with similar functions in IPF may not be enough to stop the development of fibrosis as it involves a complex network of regulation mechanisms. Intervening interleukins combined with other existing therapy or targeting interleukins affecting multiple cells/with different functions at the same time may be one of the future directions. Furthermore, the intervention time is critical as some interleukins play different roles at different stages. Further elucidation on these aspects would provide new perspectives on both the pathogenesis mechanism, as well as the therapeutic strategy and drug development.

scholarly journals China-Pakistan Economic Corridor: Analytical overview on Socio-Cultural Romance between China and Pakistan

2021 ◽  
Vol 2 (4) ◽  
pp. 492-508
Author(s):  
Muhammad Asif ◽  
Majid Ali ◽  
Abdoulaye M’Begniga ◽  
Zhou Guoqing ◽  
Liu Yang ◽  
...  
Keyword(s):  
Strong Relationship ◽  
Cultural Exchange ◽  
Vital Role ◽  
Primary Sources ◽  
Secondary Sources ◽  
Online Databases ◽  
Belt And Road ◽  
Bilateral Cooperation ◽  
The Relationship

China Pakistan Economic Corridor (CPEC) is an important Belt and Road Initiative (BRI) project that integrates many countries. The CPEC project will play a vital role to make more strong relationship between China and Pakistan. CPEC project will enhance cooperation between Pakistan and China in field of socio-cultural and regional Bilateral Cooperation and exchange. Despite growth between two countries in the economic and trade field, there is a vital area to explore the cultural exchange, cooperation, and communication needed to improve from both sides. In this theoretical study method, the data has been obtained from primary and secondary sources. The primary sources consisted of books, official databases of China and Pakistan, MoU signed b/w both countries, etc., as the secondary sources consisted of research papers, newspapers, journals, online databases, etc. This paper has an analytical overview of the time value and general cultural and artistic exchanges between China and Pakistan. Further, the paper discussed the relationship between literature, art, and economics. In this paper, researchers have also examined the significant role of people-to-people contact for socio-cultural exchange and cooperation between China and Pakistan.

Copper complexes as antitumor agents: in vitro and in vivo evidences

2021 ◽  
Vol 28 ◽  
Author(s):  
Lucia M. Balsa ◽  
Enrique J. Baran ◽  
Ignacio E. León
Keyword(s):  
Copper Complexes ◽  
Antitumor Agents ◽  
Essential Element ◽  
Design Synthesis ◽  
Copper Compounds ◽  
Comprehensive Knowledge ◽  
The Relationship

: Copper is an essential element for most aerobic organisms, with an important function as a structural and catalytic cofactor, and in consequence, it is implicated in several biological actions. The relevant aspects of chemistry and biochemistry and the importance of copper compounds in medicine give us a comprehensive knowledge of the multifaceted applications of copper in physiology and physiopathology. In this review, we present an outline of the chemistry and the antitumor properties of copper complexes on breast, colon, and lung cancer cells focus on the role of copper in cancer, the relationship between structure-activity, molecular targets, and the study of the mechanism of action involved in its anticancer activity. This overview is expected to contribute to understanding the design, synthesis, uses of copper complexes as antitumor agents in the most common cancers.

The relationship between food heritage and clan culture: is “familiness” the missing link in SMEs?

2020 ◽  
Vol 123 (1) ◽  
pp. 337-354
Author(s):  
Fahimeh Khatami ◽  
Alberto Ferraris ◽  
Paola De Bernardi ◽  
Valter Cantino
Keyword(s):  
Structural Equation ◽  
Small Sample Size ◽  
Methodological Approach ◽  
Strong Relationship ◽  
Small Sample ◽  
Equation Modeling ◽  
Content Type ◽  
Clan Culture ◽  
The Relationship

PurposeThis paper empirically tests the relationship between food heritage, familiness, and clan culture, thus, highlighting the pivotal role of familiness in building robustly competitive food firms based on clan culture and food heritage.Design/methodology/approachThe methodological approach adopted is based on a quantitative analysis with data from one eco-tourist city in Iran (Torqabeh). In this regard, we developed a structured questionnaire surveying 98 small- and medium-sized enterprises (SMEs) operating in the food industry. We then used partial least squares structural equation modeling (PLS-SEM) to carry out the analysis.FindingsThe results indicate the significant positive relationship between food heritage and clan culture, and highlight the role of familiness as a strong mediator, which is also associated with a strong relationship between food heritage and clan culture.Research limitations/implicationsIn the present study, the main limitation was linked to the small sample size and data collection, which took place in only a single city; however, further research could overcome this limitation by investigating SMEs from a heterogeneous geographical context.Originality/valueThe value of this research relates to studies that have examined food heritage as a possible antecedent of familiness. Moreover, the novelty of this research is to study the concept of familiness in improving resource-based views and organizational theories.

Mitochondrial respiration in creatine-loaded muscle: is there 31P-MRS evidence of direct effects of phosphocreatine and creatine in vivo?

2006 ◽  
Vol 100 (4) ◽  
pp. 1428-1430 ◽  
Author(s):  
Graham Kemp
Keyword(s):  
Respiration Rate ◽  
Mitochondrial Respiration ◽  
Strong Relationship ◽  
Knee Extension ◽  
Resonance Spectroscopy ◽  
Direct Effects ◽  
The Relationship ◽  
Knee Extension Exercise ◽  
Moderate Relationship

Recent human isolated muscle fiber studies suggest that phosphocreatine (PCr) and creatine (Cr) concentrations play a role in the regulation of mitochondrial respiration rate. To determine whether similar regulatory mechanisms are present in vivo, this study examined the relationship between skeletal muscle mitochondrial respiration rate and end-exercise PCr, Cr, PCr-to-Cr ratio (PCr/Cr), ADP, and pH by using 31P-magnetic resonance spectroscopy in 16 men and women (36.9 ± 4.6 yr). The initial PCr resynthesis rate and time constant (Tc) were used as indicators of mitochondrial respiration after brief (10–12 s) and exhaustive (1–4 min) dynamic knee extension exercise performed in placebo and creatine-supplemented conditions. The results show that the initial PCr resynthesis rate has a strong relationship with end-exercise PCr, Cr, and PCr/Cr ( r > 0.80, P < 0.001), a moderate relationship with end-exercise ADP ( r = 0.77, P < 0.001), and no relationship with end-exercise pH ( r = −0.14, P = 0.34). The PCr Tc was not as strongly related to PCr, Cr, PCr/Cr, and ADP ( r < 0.77, P < 0.001–0.18) and was significantly influenced by end-exercise pH ( r = −0.43, P < 0.01). These findings suggest that end-exercise PCr and Cr should be taken into consideration when PCr recovery kinetics is used as an indicator of mitochondrial respiration and that the initial PCr resynthesis rate is a more reliable indicator of mitochondrial respiration compared with the PCr Tc.

Hypothesis Testing on a Proportional Reasoning Task by Children at Different Piagetian Stage Levels

1986 ◽  
Vol 9 (1) ◽  
pp. 91-104
Author(s):  
Gerald E. Gruen ◽  
Stuart I. Offenbach ◽  
Thomas Keane
Keyword(s):  
Sex Differences ◽  
Hypothesis Testing ◽  
Proportional Reasoning ◽  
Strong Relationship ◽  
Older Children ◽  
Reasoning Task ◽  
Cognitive Limitations ◽  
The Relationship ◽  
Age And Sex

The relationship between 7-, 9-, and 11-year-old children's performance on a battery of selected Piagetian measures and on a proportional reasoning task was examined. The proportional reasoning task, modeled after an hypothesis-testing probe procedure, was devised to identify the hypotheses and strategies children use when making proportional judgments. As expected, a strong relationship between stage level and (1) the complexity of hypotheses used, (2) the use of the proportional hypothesis, and (3) the overall use of logical hypotheses was found. Generally, only formal-operational children responded proportionally. Contrary to expectations, children were not responsive to feedback, i.e., they tended to generate and maintain the same hypothesis across trials regardless of feedback. The role of children's cognitive limitations, as well as how salient the feedback was, were discussed as possible explanations for this finding. Age and sex differences on the proportional reasoning task also were found. Possible directions for extension of this study to older children and adolescents were discussed.

Relational Competence-Based Knowledge Transfer Within Intrafamily Succession

2013 ◽  
Vol 28 (1) ◽  
pp. 10-25 ◽  
Author(s):  
Isabella R. Hatak ◽  
Dietmar Roessl
Keyword(s):  
Knowledge Management ◽  
Knowledge Transfer ◽  
Transfer Process ◽  
Strong Relationship ◽  
Relational Competence ◽  
Knowledge Based ◽  
Successful Business ◽  
The Relationship

This article discusses the challenges of knowledge management within intrafamily succession against the background of the knowledge-based view. As a knowledge transfer is crucial for a successful business continuation, factors that promote the interpersonal knowledge transfer are identified. Since the quality of the relationship between successor and predecessor is considered a key determinant of knowledge transfer, the role of relational competence in the knowledge transfer process is analyzed. A laboratory experiment ( N = 107) was conducted to test the derived hypotheses. In its conclusion, the article presents the empirically confirmed strong relationship between relational competence and knowledge transfer within intrafamily succession.

Use of phosphocreatine kinetics to determine the influence of creatine on muscle mitochondrial respiration: an in vivo 31P-MRS study of oral creatine ingestion

2004 ◽  
Vol 96 (6) ◽  
pp. 2288-2292 ◽  
Author(s):  
Sinclair A. Smith ◽  
Scott J. Montain ◽  
Gary P. Zientara ◽  
Roger A. Fielding
Keyword(s):  
Respiration Rate ◽  
Mitochondrial Respiration ◽  
Strong Relationship ◽  
Knee Extension ◽  
Regulatory Mechanisms ◽  
Resonance Spectroscopy ◽  
The Relationship ◽  
Knee Extension Exercise ◽  
Moderate Relationship

Recent human isolated muscle fiber studies suggest that phosphocreatine (PCr) and creatine (Cr) concentrations play a role in the regulation of mitochondrial respiration rate. To determine whether similar regulatory mechanisms are present in vivo, this study examined the relationship between skeletal muscle mitochondrial respiration rate and end-exercise PCr, Cr, PCr-to-Cr ratio (PCr/Cr), ADP, and pH by using 31P-magnetic resonance spectroscopy in 16 men and women (36.9 ± 4.6 yr). The initial PCr resynthesis rate and time constant (Tc) were used as indicators of mitochondrial respiration after brief (10–12 s) and exhaustive (1–4 min) dynamic knee extension exercise performed in placebo and creatine-supplemented conditions. The results show that the initial PCr resynthesis rate has a strong relationship with end-exercise PCr, Cr, and PCr/Cr ( r > 0.80, P < 0.001), a moderate relationship with end-exercise ADP ( r = 0.77, P < 0.001), and no relationship with end-exercise pH ( r = -0.14, P = 0.34). The PCr Tc was not as strongly related to PCr, Cr, PCr/Cr, and ADP ( r < 0.77, P < 0.001–0.18) and was significantly influenced by end-exercise pH ( r = -0.43, P < 0.01). These findings suggest that end-exercise PCr and Cr should be taken into consideration when PCr recovery kinetics is used as an indicator of mitochondrial respiration and that the initial PCr resynthesis rate is a more reliable indicator of mitochondrial respiration compared with the PCr Tc.

scholarly journals Chemical synthesis of erythropoietin glycoforms for insights into the relationship between glycosylation pattern and bioactivity

2016 ◽  
Vol 2 (1) ◽  
pp. e1500678 ◽  
Author(s):  
Masumi Murakami ◽  
Tatsuto Kiuchi ◽  
Mika Nishihara ◽  
Katsunari Tezuka ◽  
Ryo Okamoto ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Synthesis ◽  
Glycosylation Pattern ◽  
Chemical Ligation ◽  
Homogeneous Form ◽  
Synthetic Strategy ◽  
Coupling Strategy ◽  
The Relationship

The role of sialyloligosaccharides on the surface of secreted glycoproteins is still unclear because of the difficulty in the preparation of sialylglycoproteins in a homogeneous form. We selected erythropoietin (EPO) as a target molecule and designed an efficient synthetic strategy for the chemical synthesis of a homogeneous form of five EPO glycoforms varying in glycosylation position and the number of human-type biantennary sialyloligosaccharides. A segment coupling strategy performed by native chemical ligation using six peptide segments including glycopeptides yielded homogeneous EPO glycopeptides, and folding experiments of these glycopeptides afforded the correctly folded EPO glycoforms. In an in vivo erythropoiesis assay in mice, all of the EPO glycoforms displayed biological activity, in particular the EPO bearing three sialyloligosaccharides, which exhibited the highest activity. Furthermore, we observed that the hydrophilicity and biological activity of the EPO glycoforms varied depending on the glycosylation pattern. This knowledge will pave the way for the development of homogeneous biologics by chemical synthesis.

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