scholarly journals Identification of soluble APO-1 in supernatants of human B- and T-cell lines and increased serum levels in B- and T-cell leukemias

Blood ◽  
1995 ◽  
Vol 85 (6) ◽  
pp. 1562-1569 ◽  
Author(s):  
E Knipping ◽  
KM Debatin ◽  
K Stricker ◽  
B Heilig ◽  
A Eder ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Surface Protein ◽  
Serum Levels ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Grade ◽  
Tnf Receptor ◽  
T Cell Lines ◽  
Culture Supernatants

The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously been identified for most members of the NGF/TNF receptor superfamily. Recently, a soluble form of APO-1 (sAPO- 1) was described. We established a sandwich enzyme-linked immunosorbent assay to detect sAPO-1 in culture supernatants of human cell lines and in human sera. sAPO-1 was found in culture supernatants of different human B- and T-cell lines. Molecular weights of sAPO-1 and membrane APO- 1 were similar. In addition, in comparison to healthy donors, sera from patients with different high- and low-grade malignant B- and T-cell leukemias and lymphomas contained increased levels of sAPO-1. These findings may have implications for the growth of leukemias and the diagnostic monitoring of individual patients.

scholarly journals High serum levels of soluble interleukin 2 receptor in patients with B chronic lymphocytic leukemia

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 396-400 ◽  
Author(s):  
G Semenzato ◽  
R Foa ◽  
C Agostini ◽  
R Zambello ◽  
L Trentin ◽  
...  
Keyword(s):  
T Cell ◽  
Chronic Lymphocytic Leukemia ◽  
Interleukin 2 ◽  
Serum Levels ◽  
High Serum ◽  
Lymphocytic Leukemia ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interleukin 2 Receptor

Abstract By using an enzyme-linked immunosorbent assay, the presence of the soluble form of the interleukin-2 receptor (sIL-2R) was evaluated in the peripheral blood of 54 patients with B cell chronic lymphocytic leukemia (B-CLL). Serum levels of sIL-2R were correlated with clinical features, relevant hematologic and immunological data, and in some cases, with in vitro functional studies. In 51 patients (94.4%), the levels of sIL-2R were increased as compared with normal age-matched controls (1,781 U/mL +/- 231 v 276 U/mL +/- 26, respectively; P less than .001). Although this increase was observed in all stages of the disease and independently of several hematologic and immunologic parameters, a trend toward lower levels of sIL-2R was documented in patients with a less-invasive disease. When the values were correlated with the functional status of the residual T cell population, it was found that patients with the lowest levels of sIL-2R showed the best mitogenic response and helper capacity. It is suggested that in B-CLL patients the high levels of serum sIL-2R, capable of binding to its ligand, may block the T cell-produced IL-2, thus contributing toward a defective physiological action by this lymphokine. In turn, this defective availability of IL-2 may play a part in the abnormal immunoregulation that is implicated in the hypogammaglobulinemia, susceptibility to infections, and incidence of second neoplasias often observed in this disease.

scholarly journals H2-M3 Major Histocompatibility Complex Class Ib-Restricted CD8 T Cells Induced by Salmonella enterica Serovar Typhimurium Infection Recognize Proteins Released by Salmonella Serovar Typhimurium

2005 ◽  
Vol 73 (12) ◽  
pp. 8002-8008 ◽  
Author(s):  
S. Ugrinovic ◽  
C. G. Brooks ◽  
J. Robson ◽  
B. A. Blacklaws ◽  
C. E. Hormaeche ◽  
...  
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
T Cell ◽  
Cell Lines ◽  
Cd8 T Cells ◽  
Histocompatibility Complex ◽  
Serovar Typhimurium ◽  
T Cell Lines ◽  
Culture Supernatants ◽  
Class Ib

ABSTRACT Salmonella enterica serovar Typhimurium causes a typhoid-like disease in mice which has been studied extensively as a model for typhoid fever in humans. CD8 T cells contribute to protection against S. enterica serovar Typhimurium in mice, but little is known about the specificity and major histocompatibility complex (MHC) restriction of the response. We report here that CD8 T-cell lines derived from S. enterica serovar Typhimurium-infected BALB/c mice lysed bone marrow macrophages infected with S. enterica serovar Typhimurium or pulsed with proteins from S. enterica serovar Typhimurium culture supernatants. Cytoxicity was beta-2-microglobulin dependent and largely TAP dependent, although not MHC class Ia restricted, as target cells of several different MHC haplotypes were lysed. The data suggested the participation of class Ib MHC molecules although no evidence for the presence of Qa1-restricted T cells could be found, unlike in previous reports. Instead, the T-cell lines lysed H2-M3-transfected fibroblasts infected with S. enterica serovar Typhimurium SL3261 or treated with Salmonella culture supernatants. Thus, this report increases the number of MHC class Ib antigen-presenting molecules known for Salmonella antigens to three: Qa-1, HLA-E, and now H2-M3. It also expands the range of pathogens that induce H2-M3-restricted CD8 T cells to include an example of gram-negative bacteria.

scholarly journals High serum levels of soluble interleukin 2 receptor in patients with B chronic lymphocytic leukemia

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 396-400 ◽  
Author(s):  
G Semenzato ◽  
R Foa ◽  
C Agostini ◽  
R Zambello ◽  
L Trentin ◽  
...  
Keyword(s):  
T Cell ◽  
Chronic Lymphocytic Leukemia ◽  
Interleukin 2 ◽  
Serum Levels ◽  
High Serum ◽  
Lymphocytic Leukemia ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interleukin 2 Receptor

By using an enzyme-linked immunosorbent assay, the presence of the soluble form of the interleukin-2 receptor (sIL-2R) was evaluated in the peripheral blood of 54 patients with B cell chronic lymphocytic leukemia (B-CLL). Serum levels of sIL-2R were correlated with clinical features, relevant hematologic and immunological data, and in some cases, with in vitro functional studies. In 51 patients (94.4%), the levels of sIL-2R were increased as compared with normal age-matched controls (1,781 U/mL +/- 231 v 276 U/mL +/- 26, respectively; P less than .001). Although this increase was observed in all stages of the disease and independently of several hematologic and immunologic parameters, a trend toward lower levels of sIL-2R was documented in patients with a less-invasive disease. When the values were correlated with the functional status of the residual T cell population, it was found that patients with the lowest levels of sIL-2R showed the best mitogenic response and helper capacity. It is suggested that in B-CLL patients the high levels of serum sIL-2R, capable of binding to its ligand, may block the T cell-produced IL-2, thus contributing toward a defective physiological action by this lymphokine. In turn, this defective availability of IL-2 may play a part in the abnormal immunoregulation that is implicated in the hypogammaglobulinemia, susceptibility to infections, and incidence of second neoplasias often observed in this disease.

Chemoattractant(s) in Culture Supernatants of HTLV-I-Infected T-Cell Lines

1991 ◽  
Vol 7 (7) ◽  
pp. 571-577 ◽  
Author(s):  
DANIELA SAGGIORO ◽  
JI MING WANG ◽  
MARINA SIRONI ◽  
WALTER LUINI ◽  
ALBERTO MANTOVANI ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
T Cell Lines ◽  
Culture Supernatants

scholarly journals Analysis of T cell hybridomas. II. Comparisons among three distinct types of monoclonal suppressor factors.

1981 ◽  
Vol 154 (6) ◽  
pp. 1838-1851 ◽  
Author(s):  
K Okuda ◽  
M Minami ◽  
M Furusawa ◽  
M E Dorf
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Cell Lines ◽  
Binding Specificity ◽  
Genetic Restriction ◽  
Cell Hybrids ◽  
T Cell Lines ◽  
Culture Supernatants

Five hybridoma T cell lines were prepared by fusion of Ts3 cells with the BW 5147 thymoma. The culture supernatants from these T cell hybrids contained a factor, TsF3, which specifically suppressed 4-hydroxy-3-nitrophenyl acetyl hapten (NP(-hapten cutaneous sensitivity responses. The properties of this new series of hybridoma factors was compared with those of two previously characterized types of NP-specific suppressor factors (TsF1 and TsF2). TsF3 activity was only observed if the factor was administered during the effector phases of the immune response. TsF3 bears I-J and C57BL anti-NP antibody idiotypic determinants and has binding specificity for the NP hapten. Furthermore, TsF3 does not suppress H-2 (I-J)-incompatible mice. In addition to this H-2 restriction, the monoclonal TsF3 factors also demonstrated an Igh genetic restriction. Finally, the TsF3 factors could be distinguished by their ability to suppress cyclophosphamide-treated recipients.

Identification of T-cell epitopes on E2 protein of rubella virus, as recognized by human T-cell lines and clones.

1992 ◽  
Vol 66 (11) ◽  
pp. 6788-6793 ◽  
Author(s):  
D Ou ◽  
P Chong ◽  
Y Choi ◽  
P McVeigh ◽  
W A Jefferies ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Rubella Virus ◽  
T Cell Epitopes ◽  
E2 Protein ◽  
Human T Cell ◽  
T Cell Lines
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