Influence of wet distillers grains diets on beef cattle fecal bacterial community structure

Our previous studies have elucidated that oral administration of Brassica rapa L. extract, known as Nozawana in Japan, alters immune responses and gut microbiota composition, increasing the numbers of butyrate-producing bacteria. Therefore, further investigation would help elucidate the mechanism attributable for the changes and health-promoting effects observed after B rapa L. extract ingestion. To reveal the modulation effects of fermented B. rapa L. on immune function and intestinal bacterial community structure, we conducted an intervention study with healthy volunteers followed by a mouse feeding study. The pilot intervention study was conducted for healthy volunteers aged 40–64 years under the hypothesis that the number of subjects exhibiting any change in gut microbiota in response to fermented B. rapa L. consumption may be limited. In total, 20 volunteers consumed 30 g of fermented B. rapa L. per day for 4 weeks. The fecal bacterial community composition of the volunteers was characterized using terminal-restriction fragment length polymorphism patterning followed by clustering analysis. To evaluate the detailed changes in the immune responses and the gut bacterial composition, assessed by high-throughput sequencing, we fed healthy mice with freeze-dried, fermented B. rapa L. for 2 weeks. The fecal bacterial community composition of the volunteers before the intervention was divided into three clades. Regardless of the clade, the defecation frequency significantly increased during the intervention weeks compared with that before the intervention. However, this clustering detected a specific increase of Prevotella in one cluster (low to zero Prevotella and high occupation of Clostridium at clusters IV and XIVa) post-ingestion. The cytokine production of spleen cells significantly increased due to feeding fermented B. rapa L. to the mice. This supplementary in vivo trial provided comparable results to the volunteer study regarding the effects of ingestion of the material given the compositional change complying with that of dietary fiber, particularly in the increase of genera Prevotella, Lachnospira, and genera in the Ruminococcaceae family, and the increase in daily defecation amount during 2 weeks of administration. We conclude that feeding fermented B. rapa L. may be responsible for the observed modulation in gut microbiota to increase fiber-degrading bacteria and butyrate-producing bacteria which may be relevant to the improvement in bowel function such as defecation frequency.

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A comparison of bacterial community structure, activity and microcystins associated with formation and breakdown of a cyanobacterial scum

Aquatic Microbial Ecology ◽

10.3354/ame01852 ◽

2017 ◽

Vol 80 (3) ◽

pp. 243-256 ◽

Cited By ~ 5

Author(s):

K Steiner ◽

SA Wood ◽

J Puddick ◽

I Hawes ◽

DR Dietrich ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Structure Activity

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Temporally invariable bacterial community structure in the Arabian Sea oxygen minimum zone

Aquatic Microbial Ecology ◽

10.3354/ame01704 ◽

2014 ◽

Vol 73 (1) ◽

pp. 51-67 ◽

Cited By ~ 12

Author(s):

A Jain ◽

M Bandekar ◽

J Gomes ◽

D Shenoy ◽

RM Meena ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Arabian Sea ◽

Oxygen Minimum Zone ◽

Minimum Zone ◽

Oxygen Minimum

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1103 - Remediation of Ectomycorrhiza in Heavy-Metal Soil and its Effect on Bacterial Community Structure in Rhizosphere

10.26226/morressier.5b5199c1b1b87b000ecee87b ◽

2018 ◽

Author(s):

Song Ying-Ping ◽

Yu-Ting Wu

Keyword(s):

Heavy Metal ◽

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure

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Effects of heavy metals on bacterial community structure in the rhizosphere of Salsola collina and bulk soil in the Jinchuan mining area

Geomicrobiology Journal ◽

10.1080/01490451.2021.1914784 ◽

2021 ◽

pp. 1-11

Author(s):

Tian-Peng Gao ◽

Zi-Dong Wan ◽

Xiao-Xiao Liu ◽

Jing-Wen Fu ◽

Guo-Hua Chang ◽

...

Keyword(s):

Heavy Metals ◽

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Mining Area ◽

Bulk Soil

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The root endophytic bacterial community of Ricinus communis L. resembles the seeds community more than the rhizosphere bacteria independent of soil water content

Scientific Reports ◽

10.1038/s41598-021-81551-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Stephanie E. Hereira-Pacheco ◽

Yendi E. Navarro-Noya ◽

Luc Dendooven

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Water Content ◽

Soil Water ◽

Soil Water Content ◽

Plant Development ◽

Bacterial Community Structure ◽

Ricinus Communis ◽

Ricinus Communis L ◽

Endophytic Bacterial Community

AbstractRhizosphere and root endophytic bacteria are crucial for plant development, but the question remains if their composition is similar and how environmental conditions, such as water content, affect their resemblance. Ricinus communis L., a highly drought resistant plant, was used to study how varying soil water content affected the bacterial community in uncultivated, non-rhizosphere and rhizosphere soil, and in its roots. Additionally, the bacterial community structure was determined in the seeds of R. communis at the onset of the experiment. Plants were cultivated in soil at three different watering regimes, i.e. 50% water holding capacity (WHC) or adjusted to 50% WHC every two weeks or every month. Reducing the soil water content strongly reduced plant and root dry biomass and plant development, but had little effect on the bacterial community structure. The bacterial community structure was affected significantly by cultivation of R. communis and showed large variations over time. After 6 months, the root endophytic bacterial community resembled that in the seeds more than in the rhizosphere. It was found that water content had only a limited effect on the bacterial community structure and the different bacterial groups, but R. communis affected the bacterial community profoundly.

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Bacterial community structure alterations within the colorectal cancer gut microbiome

BMC Microbiology ◽

10.1186/s12866-021-02153-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Mark Loftus ◽

Sayf Al-Deen Hassouneh ◽

Shibu Yooseph

Keyword(s):

Colorectal Cancer ◽

Community Structure ◽

Bacterial Community ◽

Gut Microbiome ◽

Late Stage ◽

Bacterial Community Structure ◽

Whole Genome ◽

Human Gut ◽

Fecal Samples ◽

Human Gut Microbiome

Abstract Background Colorectal cancer is a leading cause of cancer-related deaths worldwide. The human gut microbiome has become an active area of research for understanding the initiation, progression, and treatment of colorectal cancer. Despite multiple studies having found significant alterations in the carriage of specific bacteria within the gut microbiome of colorectal cancer patients, no single bacterium has been unequivocally connected to all cases. Whether alterations in species carriages are the cause or outcome of cancer formation is still unclear, but what is clear is that focus should be placed on understanding changes to the bacterial community structure within the cancer-associated gut microbiome. Results By applying a novel set of analyses on 252 previously published whole-genome shotgun sequenced fecal samples from healthy and late-stage colorectal cancer subjects, we identify taxonomic, functional, and structural changes within the cancer-associated human gut microbiome. Bacterial association networks constructed from these data exhibited widespread differences in the underlying bacterial community structure between healthy and colorectal cancer associated gut microbiomes. Within the cancer-associated ecosystem, bacterial species were found to form associations with other species that are taxonomically and functionally dissimilar to themselves, as well as form modules functionally geared towards potential changes in the tumor-associated ecosystem. Bacterial community profiling of these samples revealed a significant increase in species diversity within the cancer-associated gut microbiome, and an elevated relative abundance of species classified as originating from the oral microbiome including, but not limited to, Fusobacterium nucleatum, Peptostreptococcus stomatis, Gemella morbillorum, and Parvimonas micra. Differential abundance analyses of community functional capabilities revealed an elevation in functions linked to virulence factors and peptide degradation, and a reduction in functions involved in amino-acid biosynthesis within the colorectal cancer gut microbiome. Conclusions We utilize whole-genome shotgun sequenced fecal samples provided from a large cohort of late-stage colorectal cancer and healthy subjects to identify a number of potentially important taxonomic, functional, and structural alterations occurring within the colorectal cancer associated gut microbiome. Our analyses indicate that the cancer-associated ecosystem influences bacterial partner selection in the native microbiota, and we highlight specific oral bacteria and their associations as potentially relevant towards aiding tumor progression.

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