scholarly journals Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology in early diagnosis of dengue in travelers

Retrovirology ◽  
2010 ◽  
Vol 7 (S1) ◽  
Author(s):  
Eili Huhtamo
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Antigen Detection ◽  
Rt Pcr ◽  
Ns1 Antigen

Early diagnosis of dengue in travelers: Comparison of a novel real-time RT-PCR, NS1 antigen detection and serology

2010 ◽  
Vol 47 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Eili Huhtamo ◽  
Essi Hasu ◽  
Nathalie Y. Uzcátegui ◽  
Elina Erra ◽  
Simo Nikkari ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Antigen Detection ◽  
Rt Pcr ◽  
Ns1 Antigen

scholarly journals Early diagnosis of SARS Coronavirus infection by real time RT-PCR

2003 ◽  
Vol 28 (3) ◽  
pp. 233-238 ◽  
Author(s):  
Leo L.M. Poon ◽  
Kwok Hung Chan ◽  
On Kei Wong ◽  
Wing Cheong Yam ◽  
Kwok Yung Yuen ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Sars Coronavirus ◽  
Rt Pcr ◽  
Coronavirus Infection

scholarly journals Rapid SARS-CoV-2 antigen detection assay in comparison with real-time RT-PCR assay for laboratory diagnosis of COVID-19 in Thailand

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Chutikarn Chaimayo ◽  
Bualan Kaewnaphan ◽  
Nattaya Tanlieng ◽  
Niracha Athipanyasilp ◽  
Rujipas Sirijatuphat ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
False Negative ◽  
Laboratory Diagnosis ◽  
Antigen Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Screening Assay ◽  
The Real ◽  
Pcr Test

Abstract Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Performance characteristics of the rapid SARS-CoV-2 antigen detection test should be evaluated and compared with the gold standard real-time reverse transcription-polymerase chain reaction (RT-PCR) test for diagnosis of COVID-19 cases. Methods The rapid SARS-CoV-2 antigen detection test, Standard™ Q COVID-19 Ag kit (SD Biosensor®, Republic of Korea), was compared with the real-time RT-PCR test, Allplex™ 2019-nCoV Assay (Seegene®, Korea) for detection of SARS-CoV-2 in respiratory specimens. Four hundred fifty-four respiratory samples (mainly nasopharyngeal and throat swabs) were obtained from COVID-19 suspected cases and contact individuals, including pre-operative patients at Siriraj Hospital, Bangkok, Thailand during March–May 2020. Results Of 454 respiratory samples, 60 (13.2%) were positive, and 394 (86.8%) were negative for SARS-CoV-2 RNA by real-time RT-PCR assay. The duration from onset to laboratory test in COVID-19 suspected cases and contact individuals ranged from 0 to 14 days with a median of 3 days. The rapid SARS-CoV-2 antigen detection test’s sensitivity and specificity were 98.33% (95% CI, 91.06–99.96%) and 98.73% (95% CI, 97.06–99.59%), respectively. One false negative test result was from a sample with a high real-time RT-PCR cycle threshold (Ct), while five false positive test results were from specimens of pre-operative patients. Conclusions The rapid assay for SARS-CoV-2 antigen detection showed comparable sensitivity and specificity with the real-time RT-PCR assay. Thus, there is a potential use of this rapid and simple SARS-CoV-2 antigen detection test as a screening assay.

scholarly journals Six Cases of Zika/Dengue Coinfection in a Brazilian Cohort, 2015–2019

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1201
Author(s):  
Claudio Siqueira ◽  
Valéria Féres ◽  
Livia Coutinho ◽  
Isabela Junqueira ◽  
Luziane Bento ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Outcomes ◽  
Acute Infection ◽  
Warning Signs ◽  
Surveillance Systems ◽  
Rt Pcr ◽  
Zikv Infection ◽  
Central Brazil ◽  
Ns1 Antigen ◽  
Coinfected Patient

Brazil is one of the countries which has been most affected by dengue epidemics. This scenario became more challenging with the emergence of Zika virus after 2014. The cocirculation of dengue and Zika viruses makes their diagnosis and treatment a challenge for health professionals, especially due to their similar clinical outcomes. From 2015 to 2019, we followed a cohort of 2017 participants in Goiania, Goias, Central Brazil. Febrile cases were monitored weekly, and after identification of fever, the physician performed a home visit for clinical evaluation and collection of blood/urine for diagnosis of acute dengue/Zika infection in suspected cases. Dengue acute infection was investigated by NS1 antigen and real time RT-PCR and seroconversion of anti-dengue IgM. ZIKV infection was confirmed by real time RT-PCR. Six cases of Zika/dengue coinfection among participants were reported. The clinical outcomes were suggestive for both DENV and ZIKV infection. No coinfected patient had neurological clinical manifestation, warning signs or need for hospitalization. A continuous specific laboratory confirmation for both dengue and Zika viruses should be enforced as part of the surveillance systems even in the presence of very suggestive cases of dengue fever, minimizing the risk of a late detection of ZIKV circulation.

scholarly journals Comparison of nonstructural protein-1 antigen detection by rapid and enzyme-linked immunosorbent assay test and its correlation with polymerase chain reaction for early diagnosis of dengue

2017 ◽  
Vol 9 (03) ◽  
pp. 177-181 ◽  
Author(s):  
Seema Gaikwad ◽  
Sandhya S. Sawant ◽  
Jayanthi S. Shastri
Keyword(s):  
Polymerase Chain Reaction ◽  
Nonstructural Protein ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Nonstructural Protein 1 ◽  
Ns1 Antigen ◽  
Polymerase Chain ◽  
Sera Samples

Abstract INTRODUCTION: Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-time polymerase chain reaction (RT-PCR) for diagnosis of dengue. MATERIALS AND METHODS: Prospective laboratory study was carried out on sera samples (n = 200) from clinically suspected cases of dengue. The sera samples were subjected for NS1 antigen detection test by rapid test, NS1 ELISA, and RT-PCR. The results of rapid and ELISA tests were compared with real Time PCR. RESULTS: The sensitivity, specificity, positive, and negative predictive value of rapid dengue NS1 antigen test were 81.5%, 66.7%, 78.2%, and 71.1%, respectively whereas that of NS1 ELISA were 89.9%, 100%, 100%, and 94%, respectively. Concordance of Rapid NS1 and NS1 ELISA with PCR was 75.5% and 94%. DISCUSSION AND CONCLUSION: NS1 antigen ELISA can be implemented in diagnostic laboratories for diagnosis of dengue in the acute phase of illness. The test also has great potential value for use in epidemic situations, as it could facilitate the early screening of patients and limit disease expansion.

scholarly journals Molecular surveillance of Dengue in Sukabumi, West Java province, Indonesia

2014 ◽  
Vol 8 (06) ◽  
pp. 733-741 ◽  
Author(s):  
Roy Nusa ◽  
Heni Prasetyowati ◽  
Febrina Meutiawati ◽  
Benediktus Yohan ◽  
Hidayat Trimarsanto ◽  
...  
Keyword(s):  
Real Time ◽  
Evolutionary Rates ◽  
Genotype Distribution ◽  
Rt Pcr ◽  
West Java ◽  
Genotype I ◽  
Dengue Disease ◽  
Denv Serotypes ◽  
Ns1 Antigen ◽  
Serological Data

Introduction: Dengue is endemic and affects people in all Indonesian provinces. Increasing dengue cases have been observed every year in Sukabumi in West Java province. Despite the endemicity, limited data is available on the genetic of dengue viruses (DENV) circulating in the country. To understand the dynamics of dengue disease, we performed molecular and serological surveillance of dengue in Sukabumi. Methodology: A total of 113 patients were recruited for this study. Serological data were obtained using anti-dengue IgM and IgG tests plus dengue NS1 antigen detection. Dengue detection and serotyping were performed using real-time RT-PCR. Viruses were isolated and the envelope genes were sequenced. Phylogenetic and evolutionary analyses were performed to determine the genotype of the viruses and their evolutionary rates. Results: Real-time RT-PCR detected DENV in 25 (22%) of 113 samples. Serotyping revealed the predominance of DENV-2 (16 isolates, 64%), followed by DENV-1 (5 isolates, 20%), and DENV-4 (4 isolates, 16%). No DENV-3 was detected in the samples. Co-circulation of genotype I and IV of DENV-1 was observed. The DENV-2 isolates all belonged to the Cosmopolitan genotype, while DENV-4 isolates were grouped into genotype II. Overall, their evolutionary rates were similar to DENV from other countries. Conclusions: We revealed the distribution of DENV serotypes and genotypes in Sukabumi. Compared to data obtained from other cities in Indonesia, we observed the differing predominance of DENV serotypes but similar genotype distribution, where the infecting viruses were closely related with Indonesian endemic viruses isolated previously.

scholarly journals Validation of Real-Time RT-PCR for Detection of SARS-CoV-2 in the Early Stages of the COVID-19 Outbreak Without Emergency Use Authorization Reagents in the Republic of Korea

2021 ◽  
Author(s):  
Yoon-Seok Chung ◽  
Nam-Joo Lee ◽  
Sang Hee Woo ◽  
Jeong-Min Kim ◽  
Heui Man Kim ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Real Time ◽  
Target Genes ◽  
Limit Of Detection ◽  
Republic Of Korea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
The Real ◽  
Genomic Markers ◽  
The Republic

Abstract Background: A real-time reverse transcription polymerase chain reaction (RT-PCR) assay for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was developed to rapidly diagnose coronavirus disease 2019 (COVID-19). Early diagnosis of COVID-19 enables timely treatment and the implementation of public health measures. We validated the sensitivity, specificity, precision, linearity, accuracy, and robustness of a real-time RT-PCR-based assay for SARS-CoV-2 detection and compared the sensitivity and specificity of Emergency Use Authorization (EUA)-approved reagents for COVID-19 with those of the real-time RT-PCR method.Methods: The real-time RT-PCR-based assay was performed to specifically amplify genomic markers of SARS-CoV-2.Results: The real-time RT-PCR assay was highly specific for SARS-CoV-2, and did not amplify genomic fragments of 13 other viruses that cause respiratory diseases. The assay showed high linearity when conducted with a viral isolate from a patient with COVID-19 together with plasmids containing the target genes. The assay showed good repeatability and reproducibility, with a coefficient of variation of 3%, and detected SARS-CoV-2 with a limit of detection of 1 PFU/mL.Conclusions: The present real-time RT-PCR-based assay can diagnose COVID-19 with high accuracy and sensitivity. This approach is highly effective and can facilitate the early diagnosis of COVID-19 without the use of EUA reagents in the Republic of Korea.

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