Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library

ABSTRACT Antimicrobial peptides were isolated from a phage display peptide library using bacterial magnetic particles (BacMPs) as a solid support. The BacMPs obtained from “Magnetospirillum magneticum” strain AMB-1 consist of pure magnetite (50 to 100 nm in size) and are covered with a lipid bilayer membrane derived from the invagination of the inner membrane. BacMPs are easily purified from a culture of magnetotactic bacteria by magnetic separation. Approximately 4 × 1010 PFU of the library phage (complexity, 2.7 × 109) was reacted with BacMPs. The elution of bound phages from BacMPs was performed by disrupting its membrane with phospholipase D treatment. Six candidate peptides, which were highly cationic and could bind onto the BacMP membrane, were obtained. They exhibited antimicrobial activity against Bacillus subtilis but not against Escherichia coli and Saccharomyces cerevisiae. The amino acid substitution of the selected peptide, KPQQHNRPLRHK (peptide 6-7), to enhance the hydrophobicity resulted in obvious antimicrobial activity against all test microorganisms. The present study shows for the first time that a magnetic selection of antimicrobial peptides from the phage display peptide library was successfully achieved by targeting the actual bacterial inner membrane. This BacMP-based method could be a promising approach for a high-throughput screening of antimicrobial peptides targeting a wide range of species.

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Selection of CC Chemokine Receptor 5-Binding Peptide from a Phage Display Peptide Library

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.50654 ◽

2006 ◽

Vol 70 (9) ◽

pp. 2035-2041 ◽

Cited By ~ 16

Author(s):

Fang-Yu WANG ◽

Tian-Yuan ZHANG ◽

Jin-Xian LUO ◽

Guo-An HE ◽

Qu-Liang GU ◽

...

Keyword(s):

Phage Display ◽

Chemokine Receptor ◽

Peptide Library ◽

Binding Peptide ◽

Cc Chemokine ◽

Phage Display Peptide Library ◽

Cc Chemokine Receptor 5 ◽

Selection Of ◽

Chemokine Receptor 5

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Selection of Antiviral Peptides Against Mink Enteritis Virus Using a Phage Display Peptide Library

Current Microbiology ◽

10.1007/s00284-012-0284-3 ◽

2012 ◽

Vol 66 (4) ◽

pp. 379-384 ◽

Cited By ~ 8

Author(s):

Qingming Zhang ◽

Yuping Wang ◽

Qun Ji ◽

Jingmin Gu ◽

Shanshan Liu ◽

...

Keyword(s):

Phage Display ◽

Peptide Library ◽

Mink Enteritis Virus ◽

Phage Display Peptide Library ◽

Antiviral Peptides ◽

Enteritis Virus ◽

Selection Of

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EMT Participates in the Regulation of Exosomes Secretion and Function in Esophageal Cancer Cells

Technology in Cancer Research & Treatment ◽

10.1177/15330338211033077 ◽

2021 ◽

Vol 20 ◽

pp. 153303382110330

Author(s):

Chuangui Chen ◽

Zhao Ma ◽

Hongjing Jiang

Keyword(s):

Esophageal Cancer ◽

Cancer Cells ◽

Epithelial Mesenchymal Transition ◽

Promoting Effect ◽

Migration Ability ◽

Mesenchymal Transition ◽

Invasion And Migration ◽

And Migration ◽

And Function ◽

Esophageal Cancer Cells

Epithelial-mesenchymal transition (EMT) is a key step in tumor invasion and distant metastasis. Abundant evidence has documented that exosomes can mediate EMT of tumor cells and endow them with the ability of invasion and migration. However, there are few studies focusing on whether EMT can reverse the secretion of exosomes. In this study, 2 esophageal cancer cells (FLO-1 and SK-GT-4) were selected to compare the migration ability and EMT activation, and to further analyze the secretion ability of exosomes of the 2 cell lines. According to the results, inhibited activation of EMT in FLO-1 cells with relatively high migration ability could effectively reduce the secretion of exosomes. Besides, in SK-GT-4 cells, EMT activation induced by TGF-β could promote the secretion of exosomes. FLO-1 cell derived exosomes exhibited a paracrine effect of promoting the migration of SK-GT-4 cells, and the use of EMT inhibitors could weaken this ability. Furthermore, inhibition of EMT could change the relative content of some miRNAs in exosomes, with a particularly significant downregulation in the expression of miR-196-5p, miR-21-5p and miR-194-5p. Significantly, artificial transfection of the 3 miRNAs into exosomes by electroporation resulted in the recovery of migration-promoting effect of exosomes. Subsequent experiments further revealed that the effect of EMT on these miRNAs could be explained by the intracellular transcription level or the specific sorting mechanism of exosomes. To sum up, our study undoubtedly reveals that EMT has a regulatory effect on exosomes in the quantity and contents in esophageal cancer cells. Significantly, findings in our study provide experimental evidence for the interaction of EMT with the secretion and sorting pathway of exosomes, and also give a new direction for the further study of tumor metastasis.

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Isolation of phage-displayed-peptide-ligands specific to Shewanella algae cells from a phage display peptide library and application for magnetic cell separation

Journal of Japan Society of Civil Engineers Ser G (Environmental Research) ◽

10.2208/jscejer.72.iii_325 ◽

2016 ◽

Vol 72 (7) ◽

pp. III_325-III_332

Author(s):

Akinori IGUCHI ◽

Namiki SASAKI ◽

Daichi HASEGAWA ◽

Mayumi HAYASHI ◽

Hideki HARADA ◽

...

Keyword(s):

Phage Display ◽

Cell Separation ◽

Peptide Library ◽

Peptide Ligands ◽

Phage Display Peptide Library ◽

Shewanella Algae ◽

Magnetic Cell Separation ◽

I Cells

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Neddylation Inhibition Activates the Extrinsic Apoptosis Pathway through ATF4–CHOP–DR5 Axis in Human Esophageal Cancer Cells

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-15-2254 ◽

2016 ◽

Vol 22 (16) ◽

pp. 4145-4157 ◽

Cited By ~ 45

Author(s):

Ping Chen ◽

Tao Hu ◽

Yupei Liang ◽

Pei Li ◽

Xiaoyu Chen ◽

...

Keyword(s):

Esophageal Cancer ◽

Cancer Cells ◽

Apoptosis Pathway ◽

Extrinsic Apoptosis ◽

Human Esophageal Cancer ◽

Esophageal Cancer Cells

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Characteristics of P-Type and N-Type Photoelectrochemical Biosensors: A Case Study for Esophageal Cancer Detection

Nanomaterials ◽

10.3390/nano11051065 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1065

Author(s):

Joseph-Hang Leung ◽

Hong-Thai Nguyen ◽

Shih-Wei Feng ◽

Sofya B. Artemkina ◽

Vladimir E. Fedorov ◽

...

Keyword(s):

Esophageal Cancer ◽

Cancer Cells ◽

Carrier Transport ◽

X Ray Diffraction ◽

Photoelectrochemical Response ◽

Carrier Separation ◽

Human Esophageal Cancer ◽

P Type ◽

Esophageal Cancer Cells

P-type and N-type photoelectrochemical (PEC) biosensors were established in the laboratory to discuss the correlation between characteristic substances and photoactive material properties through the photogenerated charge carrier transport mechanism. Four types of human esophageal cancer cells (ECCs) were analyzed without requiring additional bias voltage. Photoelectrical characteristics were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV–vis reflectance spectroscopy, and photocurrent response analyses. Results showed that smaller photocurrent was measured in cases with advanced cancer stages. Glutathione (L-glutathione reduced, GSH) and Glutathione disulfide (GSSG) in cancer cells carry out redox reactions during carrier separation, which changes the photocurrent. The sensor can identify ECC stages with a certain level of photoelectrochemical response. The detection error can be optimized by adjusting the number of cells, and the detection time of about 5 min allowed repeated measurement.

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