Assessing the impacts of cell wall composition on the optimum stage for “Uradome” in moso bamboo

AbstractSnow damage is problematic when cultivating bamboo shoots, and “Uradome”, the practice of removing the tips of new shoots, is used as a preventative measure. Producers perform “Uradome” at empirical times, but there is no scientific basis for this. We hypothesized that differences in the structure and composition of the cell wall might affect the optimal timing of "Uradome" and analyzed the cell wall components of the “Uradome” portion. The lower plant sections broken by the “Uradome” had larger cellulose and lignin depositions than the upper sections. However, there were no differences in the lignin structure or ratio between the upper and lower nodes of the broken sections. This suggests that differences in the degree of cellulose and lignin deposition have significant effects on “Uradome” sites, and that the timing coincides with the development of one or two juvenile branches, which growers empirically consider to be the appropriate time. These results are considered to be new findings that scientifically support the cultivation management of bamboo that has been conducted empirically.

Download Full-text

CHANGES OF CELL WALL COMPONENTS AND PAL ACTIVITY IN FRESH-CUT BAMBOO SHOOTS DURING COLD STORAGE

Journal of Food Biochemistry ◽

10.1111/j.1745-4514.2004.tb00063.x ◽

2004 ◽

Vol 28 (2) ◽

pp. 169-177 ◽

Cited By ~ 4

Author(s):

YA G. XU ◽

SHENG M. LU ◽

QUN WANG

Keyword(s):

Cell Wall ◽

Cold Storage ◽

Cell Wall Components ◽

Pal Activity ◽

Bamboo Shoots ◽

Fresh Cut ◽

Wall Components

Download Full-text

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083035 ◽

1978 ◽

Vol 36 (2) ◽

pp. 434-435

Author(s):

D. Reis ◽

B. Vian ◽

J. C. Roland

Keyword(s):

Cell Wall ◽

Self Assembly ◽

Plant Cell Wall ◽

Higher Plants ◽

Three Dimensional ◽

Cytochemical Study ◽

Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Download Full-text

Mitogenic Activity of Cell Wall Components from Lactobacillus acidophilus

Nihon Chikusan Gakkaiho ◽

10.2508/chikusan.64.505 ◽

1993 ◽

Vol 64 (5) ◽

pp. 505-511 ◽

Cited By ~ 1

Author(s):

Masahiro YAMADA ◽

Haruki KITAZAWA ◽

Junko UEMURA ◽

Tadao SAITOH ◽

Takatoshi ITOH

Keyword(s):

Cell Wall ◽

Lactobacillus Acidophilus ◽

Mitogenic Activity ◽

Cell Wall Components ◽

Wall Components

Download Full-text

New insights on Laminaria digitata ultrastructure through combined conventional chemical fixation and cryofixation

Botanica Marina ◽

10.1515/bot-2021-0005 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Christos Katsaros ◽

Sophie Le Panse ◽

Gillian Milne ◽

Carl J. Carrano ◽

Frithjof Christian Küpper

Keyword(s):

Cell Wall ◽

General Structure ◽

Raw Material ◽

Rocky Shores ◽

Chemical Fixation ◽

Laminaria Digitata ◽

Vegetative Cells ◽

Biological Studies ◽

New Findings ◽

Wide Range

Abstract The objective of the present study is to examine the fine structure of vegetative cells of Laminaria digitata using both chemical fixation and cryofixation. Laminaria digitata was chosen due to its importance as a model organism in a wide range of biological studies, as a keystone species on rocky shores of the North Atlantic, its use of iodide as a unique inorganic antioxidant, and its significance as a raw material for the production of alginate. Details of the fine structural features of vegetative cells are described, with particular emphasis on the differences between the two methods used, i.e. conventional chemical fixation and freeze-fixation. The general structure of the cells was similar to that already described, with minor differences between the different cell types. An intense activity of the Golgi system was found associated with the thick external cell wall, with large dictyosomes from which numerous vesicles and cisternae are released. An interesting type of cisternae was found in the cryofixed material, which was not visible with the chemical fixation. These are elongated structures, in sections appearing tubule-like, close to the external cell wall or to young internal walls. An increased number of these structures was observed near the plasmodesmata of the pit fields. They are similar to the “flat cisternae” found associated with the forming cytokinetic diaphragm of brown algae. Their possible role is discussed. The new findings of this work underline the importance of such combined studies which reveal new data not known until now using the old conventional methods. The main conclusion of the present study is that cryofixation is the method of choice for studying Laminaria cytology by transmission electron microscopy.

Download Full-text

Ibudilast Mitigates Delayed Bone Healing Caused by Lipopolysaccharide by Altering Osteoblast and Osteoclast Activity

International Journal of Molecular Sciences ◽

10.3390/ijms22031169 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1169

Author(s):

Yuhan Chang ◽

Chih-Chien Hu ◽

Ying-Yu Wu ◽

Steve W. N. Ueng ◽

Chih-Hsiang Chang ◽

...

Keyword(s):

Cell Wall ◽

Bone Formation ◽

Cancellous Bone ◽

Bone Healing ◽

Bone Bridge ◽

Cell Wall Components ◽

Osteoclast Activity ◽

Wall Components

Bacterial infection in orthopedic surgery is challenging because cell wall components released after bactericidal treatment can alter osteoblast and osteoclast activity and impair fracture stability. However, the precise effects and mechanisms whereby cell wall components impair bone healing are unclear. In this study, we characterized the effects of lipopolysaccharide (LPS) on bone healing and osteoclast and osteoblast activity in vitro and in vivo and evaluated the effects of ibudilast, an antagonist of toll-like receptor 4 (TLR4), on LPS-induced changes. In particular, micro-computed tomography was used to reconstruct femoral morphology and analyze callus bone content in a femoral defect mouse model. In the sham-treated group, significant bone bridge and cancellous bone formation were observed after surgery, however, LPS treatment delayed bone bridge and cancellous bone formation. LPS inhibited osteogenic factor-induced MC3T3-E1 cell differentiation, alkaline phosphatase (ALP) levels, calcium deposition, and osteopontin secretion and increased the activity of osteoclast-associated molecules, including cathepsin K and tartrate-resistant acid phosphatase in vitro. Finally, ibudilast blocked the LPS-induced inhibition of osteoblast activation and activation of osteoclast in vitro and attenuated LPS-induced delayed callus bone formation in vivo. Our results provide a basis for the development of a novel strategy for the treatment of bone infection.

Download Full-text