scholarly journals Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Sang Hoon Lee ◽  
Hyun-Ouk Kim ◽  
Jung-Taek Kang
Keyword(s):  
Extracellular Matrix ◽  
Polyethylene Glycol ◽  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Culture Medium ◽  
Cross Linking ◽  
Cell Clusters ◽  
Porcine Islet ◽  
Minimal Damage ◽  
Amine Groups

AbstractResearches proving methods for nano-encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed using polymers of polyethylene glycol-block-poly lactide. Herein, our studies present efficient nano-encapsulation procedure with minimal damage and loss of NPCCs.We used N-hydroxysuccinimide (NHS) on the N-terminal of PSomes to induce binding of amine groups in the extracellular matrix surrounding NPCCs. F-10 culture medium with bovine serum albumin was used in the nano-encapsulation procedure to minimize damage and loss of NPCCs. Finally, we induced cross-linking between bifunctional PSomes (NHS-/NH2-PSomes). F-10 culture medium containing 0.25% BSA with pH of 7.3 minimized the damage and loss of NPCCs after nano-encapsulation as compared with using basic HBSS buffer (pH 8.0). Also, we induced the efficient nano-encapsulation through conjugation of PSomes using bifunctional PSomes (NHS-/NH2-PSomes).

scholarly journals Optimization of nano-encapsulation on neonatal porcine islet-like cell clusters using polymersomes

2020 ◽  
Author(s):  
Sang Hoon Lee ◽  
Hyun-Ouk Kim ◽  
Jung-Taek Kang
Keyword(s):  
Extracellular Matrix ◽  
Polyethylene Glycol ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Culture Medium ◽  
Cell Clusters ◽  
Porcine Islet ◽  
Minimal Damage ◽  
Amine Groups

Abstract ObjectiveResearches proving methods for nano encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed using polymers of polyethylene glycol-block-poly lactide (PEG-b-PLA). Herein, our studies present efficient nano encapsulation procedure with minimal damage and loss of NPCCs. MethodsWe used N-hydroxysuccinimide (NHS) on the N-terminal of PSomes to induce binding of amine groups in the extracellular matrix surrounding NPCCs. F-10 culture medium with bovine serum albumin was used in the nano-encapsulation procedure to minimize damage and loss of NPCCs. Finally, we induced crosslinking between bi-functional PSomes (NHS-/NH2-PSomes). ResultsF-10 culture medium containing 0.25% BSA with pH of 7.3 minimized the damage and loss of NPCCs after nano-encapsulation as compared with using basic HBSS buffer (pH 8.0). Also, we induced the efficiency nano encapsulation through conjugation of PSomes using bi-functional PSomes (NHS-/NH2-PSomes).

scholarly journals Bioprinting Via a Dual-Gel Bioink Based on Poly(Vinyl Alcohol) and Solubilized Extracellular Matrix towards Cartilage Engineering

2021 ◽  
Vol 22 (8) ◽  
pp. 3901
Author(s):  
Mohsen Setayeshmehr ◽  
Shahzad Hafeez ◽  
Clemens van Blitterswijk ◽  
Lorenzo Moroni ◽  
Carlos Mota ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Viability ◽  
Vinyl Alcohol ◽  
Compression Modulus ◽  
Synthetic Polymers ◽  
Poly Vinyl Alcohol ◽  
Cross Linking ◽  
Light Curing ◽  
Shape Retention ◽  
Amine Groups

Various hydrogel systems have been developed as biomaterial inks for bioprinting, including natural and synthetic polymers. However, the available biomaterial inks, which allow printability, cell viability, and user-defined customization, remains limited. Incorporation of biological extracellular matrix materials into tunable synthetic polymers can merge the benefits of both systems towards versatile materials for biofabrication. The aim of this study was to develop novel, cell compatible dual-component biomaterial inks and bioinks based on poly(vinyl alcohol) (PVA) and solubilized decellularized cartilage matrix (SDCM) hydrogels that can be utilized for cartilage bioprinting. In a first approach, PVA was modified with amine groups (PVA-A), and mixed with SDCM. The printability of the PVA-A/SDCM formulations cross-linked by genipin was evaluated. On the second approach, the PVA was functionalized with cis-5-norbornene-endo-2,3-dicarboxylic anhydride (PVA-Nb) to allow an ultrafast light-curing thiol-ene cross-linking. Comprehensive experiments were conducted to evaluate the influence of the SDCM ratio in mechanical properties, water uptake, swelling, cell viability, and printability of the PVA-based formulations. The studies performed with the PVA-A/SDCM formulations cross-linked by genipin showed printability, but poor shape retention due to slow cross-linking kinetics. On the other hand, the PVA-Nb/SDCM showed good printability. The results showed that incorporation of SDCM into PVA-Nb reduces the compression modulus, enhance cell viability, and bioprintability and modulate the swelling ratio of the resulted hydrogels. Results indicated that PVA-Nb hydrogels containing SDCM could be considered as versatile bioinks for cartilage bioprinting.

Effect of some drugs and additives on the cross-linking of bovine serum albumin by glutaraldehyde

1989 ◽  
Vol 57 (3) ◽  
pp. 205-210 ◽  
Author(s):  
A.M. Saleh ◽  
L.K. El-Khordagui ◽  
D.A. Robb ◽  
Florence A.T.
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Linking ◽  
The Cross

Bovine serum albumin contained in culture medium used in artificial insemination is an important anaphylaxis risk factor

2008 ◽  
Vol 90 (5) ◽  
pp. 2013.e17-2013.e19 ◽  
Author(s):  
Juan A. Pagán ◽  
Idoia Postigo ◽  
Jorge R. Rodríguez-Pacheco ◽  
Maribel Peña ◽  
Jorge A. Guisantes ◽  
...  
Keyword(s):  
Risk Factor ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Artificial Insemination ◽  
Bovine Serum ◽  
Culture Medium

Albumin reduces basement membrane hydraulic conductance in part due to arginyl side groups

1995 ◽  
Vol 269 (5) ◽  
pp. H1514-H1521 ◽  
Author(s):  
M. A. Katz ◽  
M. L. La Marche
Keyword(s):  
Extracellular Matrix ◽  
Bovine Serum Albumin ◽  
Basement Membrane ◽  
Serum Albumin ◽  
Protective Effect ◽  
Binding Sites ◽  
Bovine Serum ◽  
Hydraulic Conductance ◽  
Experimental Control ◽  
Low Concentrations

Albumin reduces capillary hydraulic conductance (Lp) even at low concentrations. To determine if part of this barrier protective effect might be extracellular, we studied the effects of bovine serum albumin (BSA) on Lp of self-assembled basement membrane (Matrigel). Lp with tris(hydroxymethyl)aminomethane (Tris) buffer superfusate was stable at 1.77 +/- 0.22 x 10(-5) (SE) cm.s-1.cmH2O-1 over several hours. At 0.1 g/dl BSA, experimental/control (Tris) Lp fell to 83.1 +/- 6.0% (2P < 0.025), with decreases to 72.4 +/- 3.7% at 1 g/dl (2P < 0.005), 45.3 +/- 5.1% at 2.5 g/dl (2P < 0.001), and 45.0 +/- 4.8% at 4.0 g/dl (2P < 0.001). In separate experiments, BSA arginine groups were neutralized by 1,2-cyclohexanedione (CHD), and experimental/control Lp values were measured. At 2.5 g/dl, CHD-BSA depressed Lp to 54.4 +/- 4.8%, while unmodified BSA reduced Lp to 40.8 +/- 3.5% of Tris control (2P = 0.05). Finally, soluble arginine at three- and sixfold the arginine in BSA was added to BSA superfusate. For threefold, Lp rose to 120 +/- 8% of BSA level and for sixfold to 129 +/- 9% (2P < 0.05). We conclude that some part of the albumin protective effect is very likely due to consequences on extracellular matrix and that at least 18-22% of this effect is related to arginine groups on albumin when computed from Lp, and up to 34% when viscosity is taken into account. Membrane-saturable arginine-binding sites can be unbound with arginine, thus nullifying part of the barrier protective effect of BSA.

New Photoactivators for Multiphoton Excited Three-dimensional Submicron Cross-linking of Proteins: Bovine Serum Albumin and Type 1 Collagen¶†

2007 ◽  
Vol 76 (2) ◽  
pp. 135-144
Author(s):  
Jonathan D. Pitts ◽  
Amy R. Howell ◽  
Rosa Taboada ◽  
Ipsita Banerjee ◽  
Jun Wang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Three Dimensional ◽  
Cross Linking

Combined cross-linking treatments of bovine serum albumin gel beadlets for controlled-delivery of caffeine

2009 ◽  
Vol 23 (5) ◽  
pp. 1398-1405 ◽  
Author(s):  
Chee-Yuen Gan ◽  
Lai-Hoong Cheng ◽  
Eng-Tong Phuah ◽  
Pei-Ni Chin ◽  
Abbas F.M. AlKarkhi ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Controlled Delivery ◽  
Cross Linking

scholarly journals REGULATION OF THE IMMUNE SYSTEM BY SYNTHETIC POLYNUCLEOTIDES

1972 ◽  
Vol 135 (1) ◽  
pp. 45-67 ◽  
Author(s):  
Robert D. Stout ◽  
Arthur G. Johnson
Keyword(s):  
Immune System ◽  
Bovine Serum Albumin ◽  
Antibody Production ◽  
Bovine Serum ◽  
Culture Medium ◽  
Complex Form ◽  
Spleen Cells ◽  
Fourfold Increase ◽  
Polyuridylic Acid

Addition of polyadenylic-polyuridylic acid in complex form (poly A:U) without antigen to a suspension of spleen cells obtained from BALB/Aj mice primed 6 wk previously with human γ-globulin (HGG) resulted in an immediate fourfold increase over background number of anti-HGG rosette-forming cells (RFC). Culture of similar cells in the presence of puromycin for 1–6 hr before poly A:U did not significantly reduce the response. Continued culture of primed spleen cells in the presence of poly A:U, resulted in a decrease of RFC to background levels within an hour followed by an increase again 6 hr later. This later increase in RFC was inhibited by addition of puromycin to the culture medium. The nonspecific stimulation by poly A:U of antibody production by primed spleen cells also was induced in vivo. Increases in splenic RFC were detectable 6 hr after intravenous injection of poly A:U alone, without antigen, into primed mice. The response peaked at 18 hr and had dissipated completely within 3 days. A second injection of poly A:U 24 hr or later after the first injection resulted in a second response, similar to the first with respect to kinetics and intensity. Rosette formation by poly A:U-stimulated cells could not be inhibited by mitotic poisons, but was inhibited by treatment of the cells with goat anti-mouse γ-globulin serum, suggesting that the antibody involved was a 7S γ-globulin. The decrease in RFC induced by culture of primed cells for 1 hr in poly A:U paralleled a decrease in secondary responsiveness of the cells to antigen. This poly A:U-induced inhibition of secondary responsiveness could be reversed by suspending the treated cells in supernatant fluids derived from poly A:U-stimulated cultures. The reversal was specific in that supernatant fluids removed from bovine serum albumin (BSA)-primed cells by poly A:U did not stimulate the response of HGG-primed cells to HGG. However supernatant fluids from BSA-primed cells caused the production of anti-HGG RFC if BSA rather than HGG was used as triggering antigen. The active factor in the supernatant fluids appeared to be a 7S γ-globulin since activity was lost after 45 min incubation of the supernatant fluids in the presence of goat anti-mouse 7S γ-globulin serum.

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