scholarly journals Angiotensin-converting enzyme 2 gene expression in human male urological tissues: implications for pathogenesis and virus transmission pathways

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Sammy Al-Benna
Keyword(s):  
Gene Expression ◽  
Angiotensin Converting Enzyme ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Human Lung ◽  
Converting Enzyme ◽  
Human Lung Tissue ◽  
Angiotensin Converting Enzyme 2 ◽  
Human Male ◽  
Ace2 Gene

Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurs through binding and internalization of the viral spike protein to the angiotensin-converting enzyme 2 (ACE2) receptor on the host cell membrane. Pathological changes are caused by damage and failure of vital organs that express high levels of ACE2, including the lungs, the heart and the kidneys. The aim of this study was to investigate ACE2 gene expression in the human male urogenital tract using a public database. Methods A search of transcriptomic datasets from a database to investigate ACE2 gene expression in human urogenital tract tissue. Results The gene expression profile demonstrated that ACE2 gene expression was higher in human kidney cortex and testis than human lung tissue. The gene expression profile demonstrated that ACE2 gene expression in the human bladder and prostate was comparable to human lung tissue. Conclusions Male urogenital tissues are directly susceptible to SARS-CoV-2 infection through the expression of ACE2. Moreover, the SARS-Cov-2/ACE2 interaction may disturb the male genital and reproductive functions.

scholarly journals Review: Pengaruh Tanaman Obat Yang Beraktivitas Hipertensi Terhadap Ekspresi Gen Reseptor ACE-1 dan ACE 2

2021 ◽  
Vol 7 (1) ◽  
pp. 9-31
Author(s):  
Nisa Nur Afifah ◽  
Yani Mulyani ◽  
Ari Yuniarto
Keyword(s):  
Gene Expression ◽  
Medicinal Plant ◽  
Angiotensin Converting Enzyme ◽  
Allium Cepa ◽  
Allium Sativum ◽  
Ace Inhibitor ◽  
Hibiscus Sabdariffa ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Plant Gene Expression

Hipertensi adalah salah satu penyakit dengan angka kesakitan dan kematian yang terus meningkat, termasuk di Indonesia. Dalam mengatasi hipertensi obat-obatan seperti ACE inhibitor berperan dalam menurunkan tekanan darah diastol dan sistol, namun tanaman obat seperti ekstrak buah hawthorn, buah zaitun (Olea europaea L.), Hibiscus Sabdariffa, Allium Sativum dan Allium Cepa juga memiliki efek sebagai antihipertensi dengan harga yang relatif murah, mudah didapat, efek samping yang lebih rendah dibandingkan dengan obat sintesis atau kimia lainnya. Review jurnal ini ditujukan untuk mengetahui berbagai tanaman obat yang memiliki aktivitas hipertensi dan berpengaruh terhadap ekspresi gen reseptor hipertensi ACE1 dan ACE2. Penelusuran referensi dilakukan melalui database PubMed, Science Direct, dan Google Scholar, dengan kata kunci “Medicinal Plant”, “Gene expression”, “Angiotensin Converting Enzyme 1”, “Angiotensin Converting Enzyme 2”, dan“Antihypertension”. Tanaman obat digunakan sebagai terapi alternatif penurun tekanan darah tinggi dan merupakan salah satu cara pengobatan non farmakologis hipertensi. Hal ini menunjukkan bahwa tanaman obat memiliki pengaruh dalam menurunkan tekanan darah tinggi karena kandungan senyawa yang ada dalam masing-masing tanaman sehingga mampu menghambat reseptor hipertensi ACE1 dan ACE2 dengan berbagai metode ekspresi gen. Banyak tanaman obat yang telah diteliti memiliki aktivitas sebagai antihipertensi. Dari 14 tanaman obat dengan aktivitas sebagai anti hipertensi, sebanyak 90% tanaman berpengaruh terhadap ekspresi gen Angiotensin Converting Enzyme 1 (ACE 1), dan sebanyak 10% tanaman memiliki pengaruh terhadap Angiotensin Converting Enzyme 2 (ACE 2). Tanaman obat yang telah ditemukan dan memiliki aktivitas terhadap ekspresi gen Angiotensin Converting Enzyme 1 (ACE 1) paling banyak merupakan tanaman obat dengan family Poaceae, Oleaceae, dan Zingiberaceae.

scholarly journals Gene transfer of angiotensin-converting enzyme 2 in the nucleus tractus solitarius improves baroreceptor heart rate reflex in spontaneously hypertensive rats

2011 ◽  
Vol 12 (4) ◽  
pp. 456-461 ◽  
Author(s):  
Masanobu Yamazato ◽  
Anderson J Ferreira ◽  
Yoriko Yamazato ◽  
Carlos Diez-Freire ◽  
Lihui Yuan ◽  
...  
Keyword(s):  
Heart Rate ◽  
Gene Transfer ◽  
Angiotensin Converting Enzyme ◽  
Spontaneously Hypertensive Rats ◽  
Nucleus Tractus Solitarius ◽  
Converting Enzyme ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Angiotensin Converting Enzyme 2 ◽  
Ace2 Gene

The renin–angiotensin system (RAS) in the nucleus tractus solitarius (NTS) is an important modulator of the baroreceptor heart rate reflex. This study tested the hypothesis that angiotensin-converting enzyme 2 (ACE2) expression is decreased in the NTS of spontaneously hypertensive rats (SHRs) and that its gene transfer in this nucleus would lead to beneficial effects on baroreflex function since this enzyme is key in the regulation of the vasoprotective axis of the RAS. ACE2 protein levels and its activity were significantly decreased in the NTS of SHRs compared to normotensive Wistar-Kyoto (WKY) control rats. Rats instrumented with radio-telemetry transducers received NTS microinjection of either Lenti-ACE2 (Lentiviral vector-mediated gene transfer of ACE2) or lenti-GFP (green fluorescent protein). The ACE2 gene transfer into the NTS resulted in long-term overexpression of ACE2. This was associated with a 60% increase in heart rate baroreflex sensitivity in the lenti-ACE2 injected SHRs compared with the lenti-GFP injected control SHRs (0.27 ± 0.02 ms/mmHg in lenti-GFP rats vs. 0.44 ± 0.07 ms/mmHg in lenti-ACE2 rats). These observations demonstrate that ACE2 gene transfer overcomes its intrinsic decrease in the NTS of SHRs and improves baroreceptor heart rate reflex.

Abstract MP086: Association Between the Angiotensin-Converting Enzyme-2 Gene and Blood Pressure in a Cohort of Canadian Adolescents

Circulation ◽  
2012 ◽  
Vol 125 (suppl_10) ◽  
Author(s):  
Lucile Malard ◽  
Lisa Kakinami ◽  
Jennifer O’Loughlin ◽  
Marie-Hélène Roy-Gagnon ◽  
Aurélie Labbe ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Linkage Disequilibrium ◽  
Angiotensin Converting Enzyme ◽  
Growth Models ◽  
Nucleotide Polymorphisms ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Link Type ◽  
Oscillometric Device ◽  
Ace2 Gene

Background: The Angiotensin-Converting Enzyme-2 (ACE2) gene, located on chromosome X, is believed to be implicated in blood pressure (BP) regulation. However the few studies that have assessed this association have yielded mixed results. We examined the association between BP and ACE2 single nucleotide polymorphisms (SNPs) in a population-based cohort of adolescents, thereby minimizing confounding by treatment for hypertension and other chronic diseases. Methods: Participants were 852 of 1293 adolescents from the Nicotine Dependence In Teens (NDIT) cohort study. Participants provided a blood sample for DNA, and BP was measured on the right arm at rest, in the sitting position, by trained, certified technicians using an automated oscillometric device at age 12, 15 and 17 years. Instruments were calibrated against mercury sphygmomanometer before each data collection. Systolic (SBP) and diastolic blood pressure (DBP) were measured 3 times at 1-minute intervals, and the mean was used for analysis. Four SNPs (rs2074192, rs233575 , rs2158083, rs1978124) in the ACE2 gene were genotyped. The relationship between ACE2 SNPs and each of SBP and DBP was examined using linear growth models adjusted for height and ethnicity, and stratified by sex. Results: Mean age at baseline was 12.7 years; 47.8% of participants were male (n=407 of 852). All SNPs were in Hardy-Weinberg equilibrium (p>0.05), and three of the four SNPs were in linkage disequilibrium ( rs233575 and rs2158083 r2: 0.71; rs1978124 and rs233575 r2: 0.36; rs2158083 and rs1978124 r2: 0.52) with each other. SNP rs2074192 was in low linkage disequilibrium with these 3 SNPs (r2 <0.3). Compared to boys carrying the G allele, SBP in boys carrying the A allele of SNP rs2074192 was 2.45 mmHg lower (CI: 0.20-4.70, p=0.0350); DBP was 1.67 mmHg lower (CI: 0.47-2.87, p=0.0067). SNPs rs233575 , rs2158083, and rs1978124 were not significantly associated with SBP or DBP in boys. None of the four SNPs were associated with SBP or DBP in girls. Conclusion: Because elevated BP tracks from childhood to adulthood, the ACE2 gene could represent a new therapeutic target in boys to prevent elevated BP. Association between this gene and BP and potential for prevention should be further investigated.

Gene Expression Profile of Ischemia-reperfusion Injury in Human Lung Transplantatio

CHEST Journal ◽  
2003 ◽  
Vol 124 (4) ◽  
pp. 99S ◽  
Author(s):  
Eric Olson ◽  
Jianping Jin ◽  
William Funkhouser ◽  
Yuechuan Yuan ◽  
Scott Randell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reperfusion Injury ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Human Lung ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion
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