Asymptomatic Bacteriuria, antimicrobial susceptibility pattern and associated risk factors among pregnant women attending antenatal care in Assosa General Hospital, Western Ethiopia

Abstract Background Asymptomatic bacteriuria is a common problem in pregnant women and about 40% of women with untreated asymptomatic bacteriuria during pregnancy develop pyelonephritis, which might lead to low birth weight, premature rupture of membranes, and preterm labour. Therefore, this study aimed to assess the prevalence of asymptomatic bacteriuria, antimicrobial susceptibility pattern of the isolates among pregnant women attending the antenatal care of Assosa general hospital, western Ethiopia. Methods A facility-based cross-sectional study was conducted from January to February 2019. Two hundred and eighty-three pregnant women with no symptoms of urinary tract infections participated in the study. Bacterial isolates were identified as per the standard bacteriological procedure using colony characteristics, Gram-staining, and series of biochemical tests. Antimicrobial susceptibility test was carried out by Kirby- Bauer disk diffusion technique on Muller-Hinton agar medium and the diameter of zone of inhibition was interpreted according to Clinical Laboratory Standard Institute guidelines. Results The overall prevalence of asymptomatic bacteriuria among pregnant women in this study was 13.78% (i.e. 39 out of 283 urine samples were positive for bacterial isolates). E. coli was the most predominant isolate (53.8%) followed by K. pneumoniae (17.95%), S. aureus (15.4%), and coagulase-negative staphylococci (12.8%). Gram-negative bacteria were highly resistant to tetracycline (96.4%), and ampicillin (90.5%). Conclusion Significant bacteriuria was observed in asymptomatic pregnant women. A large number of the bacterial isolates were resistant to the commonly used antimicrobial drugs.

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Asymptomatic Bacteriuria Bacterial Profile and Antibiotic Susceptibility Pattern Among Pregnant Women Attending Antenatal Care (Anc) Clinic of Assosa General Hospital, Benishangul Gumuz Region, Western Ethiopia

10.21203/rs.3.rs-100055/v1 ◽

2020 ◽

Author(s):

Duresa Abu Tusuna ◽

Teferra Abula Orjino

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

General Hospital ◽

Antimicrobial Susceptibility ◽

Asymptomatic Bacteriuria ◽

Susceptibility Pattern ◽

Group B Streptococci ◽

Biochemical Tests ◽

Care Clinic ◽

Western Ethiopia

Abstract Background: ASB is a common problem in pregnant women and about 40% of women with untreated asymptomatic bacteriuria during pregnancy develop pyelonephritis, which might lead to low birth weight, preterm, premature rupture of membranes and preterm labour. Therefore, this study aimed to assess the prevalence of asymptomatic bacteriuria, antimicrobial susceptibility pattern of the isolates among pregnant women attending antenatal care clinic of Assosa General Hospital, western Ethiopia.Methods: A facility-based cross sectional study was conducted from January to February 2019. Well-mixed and uncentrifuged urine specimens obtained from the pregnant women was directly inoculated on cystine lactose electrolyte deficient agar (CLED) (Oxoid, Ltd, England) media by streak plate method. Bacterial isolates were identified as per the standard bacteriological procedure using colony characteristics, gram-staining, and series of biochemical tests. Antimicrobial susceptibility test was carried out by Kirby- Bauer disk diffusion technique on Muller-Hinton agar medium and the diameter of zone of inhibition was interpreted according toClinical Laboratory Standard Institute (CLSI) guidelinesResult: The overall prevalence of ASB among pregnant women in this study was 14.85%.E. coli was the most predominant isolate (50 %,) followed by K. pneumoniae (16.7%,), S. aureus (14.3%), coagulase negative staphylococci (CONS) (11.9%), and group B streptococci (GBS) (7.1%).Gram-negative bacteria were highly resistant for tetracycline (96.4%), ampicillin .Gram-positive bacteria were 100% sensitive for ceftazidime. Conclusion: Significant bacteriuria was observed in asymptomatic pregnant women. Therefore, routine laboratory diagnosis of ASB in pregnant women and providing appropriate treatment should be needed to reduce its complications.

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Asymptomatic Bacteriuria among Pregnant Women Attending Antenatal Care at Hiwot Fana Specialized University Hospital, Harar, Eastern Ethiopia: Magnitude, Associated Factors, and Antimicrobial Susceptibility Pattern

International Journal of Microbiology ◽

10.1155/2020/1763931 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Mekuria Edae ◽

Zelalem Teklemariam ◽

Fitsum Weldegebreal ◽

Degu Abate

Keyword(s):

Pregnant Women ◽

Antimicrobial Susceptibility ◽

Associated Factors ◽

Asymptomatic Bacteriuria ◽

University Hospital ◽

Susceptibility Pattern ◽

Eastern Ethiopia ◽

Antimicrobial Susceptibility Pattern ◽

Tract Infections ◽

Higher Sensitivity

Background. Asymptomatic bacteriuria is one of the major risk factors for the development of urinary tract infections during pregnancy which accounts for about 70% of the cases. However, there is no guideline which recommends routine screening of pregnant women for asymptomatic bacteriuria in most of developing countries including Ethiopia. Thus, the aim of this study was to determine the magnitude, associated factors, and antimicrobial susceptibility pattern of asymptomatic bacteriuria among pregnant women. Methods. A cross-sectional study was conducted from March to April 2019. Data were collected through face-to-face interview and analyzed using Statistical Package for Social Science version 22. A test of association was performed using logistic regression and P value less than 0.05 was considered statistically significant. Results. The overall prevalence of asymptomatic bacteriuria was 19.9%. Direction of wiping after genital wash, postcoital urination, and catheterization were factors significantly associated with asymptomatic bacteriuria. Most of the isolated Gram positive were highly sensitive to Ceftriaxone (90.9%). Coagulase-negative staphylococci showed higher sensitivity to Augmentin (75.0) and Ceftriaxone (87.5%), whereas they showed resistance to Clindamycin (68.7%) and Ampicillin (62.5%). Gram-negative bacteria isolates showed higher sensitivity to Ceftriaxone (88.2%), Gentamycin (67.5%), and Augmentin (64.7%), while they showed resistance to Ampicillin (70.5%) and Clindamycin (50.0%). Conclusion. The overall prevalence of asymptomatic bacteriuria among pregnant women in this study was high. Direction of wiping after genital wash, catheterization, and postcoital urination increases the odds of asymptomatic bacteriuria. Therefore, health education on the predisposing factors is strongly recommended.

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Asymptomatic Bacteriuria Bacterial Profile and Antibiotic Susceptibility Pattern Among Pregnant Women Attending Antenatal Care (Anc) Clinic of Assosa General Hospital, Benishangul Gumuz Region, Western Ethiopia

10.21203/rs.3.rs-138813/v1 ◽

2021 ◽

Author(s):

Duresa Abu Tusuna

Keyword(s):

Pregnant Women ◽

General Hospital ◽

Antimicrobial Susceptibility ◽

Clinical Laboratory ◽

Clinical Care ◽

Asymptomatic Bacteriuria ◽

Susceptibility Pattern ◽

Group B Streptococci ◽

Biochemical Tests ◽

Western Ethiopia

Abstract Background: ASB is a common problem in pregnant women. About 40% of women with untreated asymptomatic bacteriuria during pregnancy develop pyelonephritis, this has the possibility of leading to low birth weight, preterm, premature rupture of membranes and preterm labour. This study is aimed to assess the prevalence of asymptomatic bacteriuria and antimicrobial susceptibility pattern isolating among pregnant women attending the antenatal clinical care of Assosa General Hospital in western Ethiopia.Methods: A facility-based cross-sectional study was conducted from January to February 2019. Well-mixed and uncentrifuged urine specimens obtained from the pregnant women were directly inoculated on cystine lactose electrolyte deficient agar (CLED) (Oxoid, Ltd, England) media by streak plate method. Bacterial isolates were identified as per the standard bacteriological procedure using colony characteristics, gram-staining, and series of biochemical tests. Antimicrobial susceptibility test was carried out by Kirby- Bauer disk diffusion technique on Muller-Hinton agar medium and the diameter of zone of inhibition was interpreted according to Clinical Laboratory Standard Institute (CLSI) guidelinesResult: The overall prevalence of ASB among pregnant women in this study was 14.85%. E-coli was the most predominant isolate (50 %,) followed by K. pneumoniae (16.7%,), S. aureus (14.3%), coagulase-negative staphylococci (CONS) (11.9%), and group B streptococci (GBS) (7.1%). Gram-negative bacteria were highly resistant for tetracycline (96.4%), ampicillin. Gram-positive bacteria were 100% sensitive for ceftazidime. Conclusion: Significant bacteriuria was observed in asymptomatic pregnant women. Therefore, routine laboratory diagnosis of ASB in pregnant women in addition to providing appropriate treatment should be needed to reduce its complications.

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