scholarly journals The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dan Wang ◽  
Zhanmin Sun ◽  
Xinxu Hu ◽  
Junbo Xiong ◽  
Lizhen Hu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salt Tolerance ◽  
Lipid Transfer Protein ◽  
Quantitative Polymerase Chain Reaction ◽  
Reactive Oxygen ◽  
Lotus Corniculatus ◽  
Ethylene Response Factor ◽  
Oxygen Species ◽  
Lipid Transfer ◽  
Salt Treatment

Abstract Background The APETALA2/ethylene response factor (AP2/ERF) family are important regulatory factors involved in plants’ response to environmental stimuli. However, their roles in salt tolerance in Lotus corniculatus remain unclear. Results Here, the key salt-responsive transcription factor LcERF056 was cloned and characterised. LcERF056 belonging to the B3–1 (IX) subfamily of ERFs was considerably upregulated by salt treatment. LcERF056-fused GFP was exclusively localised to nuclei. Furthermore, LcERF056- overexpression (OE) transgenic Arabidopsis and L. corniculatus lines exhibited significantly high tolerance to salt treatment compared with wild-type (WT) or RNA interference expression (RNAi) transgenic lines at the phenotypic and physiological levels. Transcriptome analysis of OE, RNAi, and WT lines showed that LcERF056 regulated the downstream genes involved in several metabolic pathways. Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) and yeast one-hybrid (Y1H) assay demonstrated that LcERF056 could bind to cis-element GCC box or DRE of reactive oxygen species (ROS)-related genes such as lipid-transfer protein, peroxidase and ribosomal protein. Conclusion Our results suggested that the key regulator LcERF056 plays important roles in salt tolerance in L. corniculatus by modulating ROS-related genes. Therefore, it may be a useful target for engineering salt-tolerant L. corniculatus or other crops.

scholarly journals Transcriptomic Analysis Reveals That Reactive Oxygen Species and Genes Encoding Lipid Transfer Protein Are Associated with Tobacco Hairy Root Growth and Branch Development

2014 ◽  
Vol 27 (7) ◽  
pp. 678-687 ◽  
Author(s):  
Jung-Hao Wang ◽  
Hsiao-Han Lin ◽  
Chi-Te Liu ◽  
Ta-Chung Lin ◽  
Li-yu Daisy Liu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Root Growth ◽  
Hairy Root ◽  
Hairy Roots ◽  
Lipid Transfer Protein ◽  
Reactive Oxygen ◽  
Rol Genes ◽  
Oxygen Species ◽  
Lipid Transfer ◽  
Genes Encoding

The hairy root, a specialized plant tissue that emerges from a cell transformed with transfer DNA (T-DNA) from Agrobacterium rhizogenes, can be used to study root biology and utilized in biotechnological applications. The rol genes are known to participate in the generation of hairy roots; however, the means by which the rol genes contribute to the initiation and the maintenance of hairy roots remains largely unknown. We demonstrated that tobacco hairy roots lacking either rolB or rolC exhibited fewer branch roots and lost their growth ability in long-term subculture. Additionally, a microarray analysis revealed that the expression of several genes encoding lipid transfer proteins (LTP) and reactive oxygen species (ROS)-related genes was significantly suppressed in rolB- or rolC-deficient hairy roots. We found that hairy root clones that exhibited greater branching expressed higher levels of RolB or RolC and the genes encoding LTP identified from the microarray. When hairy roots were compared with intact roots, the expression levels of LTP-encoding genes were dramatically different. In addition, ROS were present at lower levels in rolB- and rolC-deficient hairy roots. We therefore suggest that upregulating LTP and increasing the level of ROS is important for hairy root growth.

Zinc improves salt tolerance by increasing reactive oxygen species scavenging and reducing Na+ accumulation in wheat seedlings

2014 ◽  
Vol 58 (4) ◽  
pp. 751-757 ◽  
Author(s):  
L. H. Xu ◽  
W. Y. Wang ◽  
J. J. Guo ◽  
J. Qin ◽  
D. Q. Shi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salt Tolerance ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Wheat Seedlings

scholarly journals The Synthesis of Ascorbic Acid in Rice Roots Plays an Important Role in the Salt Tolerance of Rice by Scavenging ROS

2018 ◽  
Vol 19 (11) ◽  
pp. 3347 ◽  
Author(s):  
Yayun Wang ◽  
Hui Zhao ◽  
Hua Qin ◽  
Zixuan Li ◽  
Hai Liu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Ascorbic Acid ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Rice Roots ◽  
Key Factor ◽  
Rna Interfering

The root plays an important role in the responses of plants to stresses, but the detailed mechanisms of roots in stress responses are still obscure. The GDP-mannose pyrophosphate synthetase (GMPase) OsVTC1-3 is a key factor of ascorbic acid (AsA) synthesis in rice roots. The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves. Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress. The roots of OsVTC1-3 RI plants rapidly produced more O2−, and later accumulated amounts of H2O2 under salt stress, indicating the impaired tolerance of OsVTC1-3 RI plants to salt stress due to the decreasing ability of scavenging reactive oxygen species (ROS). Moreover, exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants, indicating that the AsA synthesis in rice roots is an important factor for the response of rice to salt stress. Further studies showed that the salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants. The above results showed that specifically regulating AsA synthesis to scavenge ROS in rice roots was one of important factors in enhancing the tolerance of rice to salt stress.

scholarly journals Ethylene Response Factor 6 Is a Regulator of Reactive Oxygen Species Signaling in Arabidopsis

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e70289 ◽  
Author(s):  
Nasser Sewelam ◽  
Kemal Kazan ◽  
Skye R. Thomas-Hall ◽  
Brendan N. Kidd ◽  
John M. Manners ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Oxygen Species ◽  
Ethylene Response ◽  
Reactive Oxygen Species Signaling

scholarly journals An Enhancer Mutant of Arabidopsis salt overly sensitive 3 Mediates both Ion Homeostasis and the Oxidative Stress Response

2007 ◽  
Vol 27 (14) ◽  
pp. 5214-5224 ◽  
Author(s):  
Jianhua Zhu ◽  
Xinmiao Fu ◽  
Yoon Duck Koo ◽  
Jian-Kang Zhu ◽  
Francis E. Jenney ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Pdz Domain ◽  
Ion Homeostasis ◽  
Reactive Oxygen ◽  
Salt Sensitivity ◽  
Oxygen Species ◽  
Isolation And Characterization

ABSTRACT The myristoylated calcium sensor SOS3 and its interacting protein kinase, SOS2, play critical regulatory roles in salt tolerance. Mutations in either of these proteins render Arabidopsis thaliana plants hypersensitive to salt stress. We report here the isolation and characterization of a mutant called enh1-1 that enhances the salt sensitivity of sos3-1 and also causes increased salt sensitivity by itself. ENH1 encodes a chloroplast-localized protein with a PDZ domain at the N-terminal region and a rubredoxin domain in the C-terminal part. Rubredoxins are known to be involved in the reduction of superoxide in some anaerobic bacteria. The enh1-1 mutation causes enhanced accumulation of reactive oxygen species (ROS), particularly under salt stress. ROS also accumulate to higher levels in sos2-1 but not in sos3-1 mutants. The enh1-1 mutation does not enhance sos2-1 phenotypes. Also, enh1-1 and sos2-1 mutants, but not sos3-1 mutants, show increased sensitivity to oxidative stress. These results indicate that ENH1 functions in the detoxification of reactive oxygen species resulting from salt stress by participating in a new salt tolerance pathway that may involve SOS2 but not SOS3.

scholarly journals Inositol Polyphosphate 5-Phosphatase7 Regulates the Production of Reactive Oxygen Species and Salt Tolerance in Arabidopsis

2011 ◽  
Vol 157 (1) ◽  
pp. 229-241 ◽  
Author(s):  
Yuval Kaye ◽  
Yael Golani ◽  
Yaniv Singer ◽  
Yehoram Leshem ◽  
Gil Cohen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salt Tolerance ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inositol Polyphosphate
Sign in / Sign up

Export Citation Format

Share Document