scholarly journals Evaluation of expression and serum concentration of anti-Mullerian hormone as a follicle growth marker following consumption of fennel and flaxseed extract in first-generation mice pups

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fahimeh Pourjafari ◽  
Tahereh Haghpanah ◽  
Fariba Sharififar ◽  
Seyed Noreddin Nematollahi-Mahani ◽  
Ali Afgar ◽  
...  
Keyword(s):  
Serum Level ◽  
Serum Concentration ◽  
First Generation ◽  
Marked Decrease ◽  
Primordial Follicle ◽  
Ovarian Follicles ◽  
Lactation Period ◽  
Gene And Protein Expression ◽  
Flaxseed Extract ◽  
Generation Female

Abstract Background The aim of the present study was to assess the expression and serum level of AMH in first-generation female mice pups following fennel and flaxseed consumption. Methods Twenty pregnant NMRI mice were allocated into four groups including control (CTL), fennel (FV), flaxseed (LU) and FV+ LU. Sixty-four female offsprings after lactation period, received the same regimen as their mothers for 56 and 240 days. The ovarian follicles development, serum concentration of AMH, as well as gene and protein expression of AMH were evaluated in the female offsprings at post-natal day 56 (PND56) and 240 (PND240). Results The number of total growing follicles were raised in the FV group in compression to the all experimental groups. In contrast, LU group showed a marked decrease in their numbers. The highest level of serum AMH was seen in the FV-diet mice, whereas LU negatively affected it. The expression level of AMH also increased in the FV and FV + LU groups, while a reduction was observed in the LU group. As well, IHC data showed that the number of AMH-positive cells in almost ovarian follicles of FV and FV + LU-treated mice was in compared to those of the LU group. Conclusions The overall effect of fennel treatment (alone and in combination with flaxseed) on ovary might be maintain primordial follicle storage through increased expression and serum level of AMH.

scholarly journals Interrogating Conflicting Narratives of Writing in the Academy: A Call for Research

2017 ◽  
Vol 27 ◽  
pp. 13-18
Author(s):  
Katie Byrant
Keyword(s):  
First Generation ◽  
Academic Work ◽  
Writing Studies ◽  
University Student ◽  
Safe Haven ◽  
Feminist Politics ◽  
Generation Female ◽  
Subjective Identity

A safe haven in an often unsafe place: I would use this metaphor to describe the space writing studies and a university writing centre have offered me, as I’ve attempted to find my own place as a feminist in the academy. I feel these two things are my rocks. They are firm, solid places for me to reside amongst the challenges I’ve experienced as a writer. The reasons for my struggles with writing for academic purposes are difficult to pinpoint. Some would say they stem from my lack of literacy, hinting that laziness could be a culprit. Others might suggest they are connected to my subjective identity as a first-generation, female university student. Or others might take the discussion of subjective identity a bit further, arguing that my identity as a feminist, and my determination to bring my feminist politics into my academic work explain these challenges.

scholarly journals Evaluation of Coronary Artery Calcification and Gremlin-1 Serum Level Correlation in Patients with Chronic Ischemic Heart Disease

2021 ◽  
Author(s):  
Atefeh Rezapour ◽  
Saeed Nazemi ◽  
Fatemeh Mashhadi ◽  
Seyed Mohammad Hassan Moallem ◽  
Mohammad Afshar ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Serum Level ◽  
Serum Concentration ◽  
Bone Morphogenetic Proteins ◽  
Coronary Artery Calcification ◽  
Positive Family History ◽  
Calcium Score ◽  
Sex Risk ◽  
Artery Disease ◽  
Gremlin 1

Background: Available evidences have shown that bone morphogenetic proteins (BMPs), particularly BMP 2 and BMP 4, are involved in vascular calcification. Gremlin 1 is one of the important endogenous inhibitors of BMPs. This extracellular antagonist of bone morphogenic proteins has a very complex and cysteine-rich chain and establishes non-colonial transmissions to the members of the family with varying degrees of dependence and prevents them from binding to the receptor, thereby inhibit their function. The main objective of this study was clinical evaluation of the correlation between Gremlin-1 serum concentration and Coronary Artery Calcification. Methods: Eighty-four patients with coronary artery disease from cardiology ward of Razavi Hospital, Mashhad, Iran, who completed the inclusion criteria, entered in the study between November 2015 and March 2016. CT-Angiography was performed to define coronary artery calcium score and Gremlin-1 serum concentrations were measured by an ELISA kit. Results: Eighty-one patients, with mean age of 57.19±10.18 years were included to the study. The mean serum level of Gremlin-1 was 10.92±8.46 pg/mL. There was a reverse significant correlation between Gremlin-1 serum concentration and Coronary Artery Calcification of Right coronary artery(RCA) (P<0.05), in contrast to total Coronary Artery Calcification score, left artery Descending (LAD), Left Main (LM) and Circumflex (CX). However, there was no evidence that age and sex risk factor, hypertension, diabetes mellitus, hyperlipidemia, positive family history, current smoking and high BMI to be associated with serum level of Gremlin-1. Conclusion:  Based on the results, Gremlin-1 serum concentration may be a suitable biomarker for predication coronary artery calcification severity. However, more researches on larger population are necessary for its validation.

Maternal l-proline supplementation during gestation alters amino acid and polyamine metabolism in the first generation female offspring of C57BL/6J mice

Amino Acids ◽  
2019 ◽  
Vol 51 (5) ◽  
pp. 805-811 ◽  
Author(s):  
Ning Liu ◽  
Zhaolai Dai ◽  
Yunchang Zhang ◽  
Hai Jia ◽  
Jiangqing Chen ◽  
...  
Keyword(s):  
Amino Acid ◽  
First Generation ◽  
Female Offspring ◽  
Polyamine Metabolism ◽  
Generation Female

scholarly journals Maternal malnutrition during lactation alters the folliculogenesis and gonadotropins and estrogen isoforms ovarian receptors in the offspring at puberty

2008 ◽  
Vol 198 (3) ◽  
pp. 625-634 ◽  
Author(s):  
Tatiane da Silva Faria ◽  
Flávia de Bittencourt Brasil ◽  
Francisco J B Sampaio ◽  
Cristiane da Fonte Ramos
Keyword(s):  
Serum Concentration ◽  
Primordial Follicle ◽  
Free Access ◽  
Standard Laboratory ◽  
Restricted Diet ◽  
Maternal Malnutrition ◽  
Protein Energy ◽  
Standard Laboratory Diet ◽  
Protein Restricted Diet

In this study, we aimed to evaluate whether maternal malnutrition during lactation alters the folliculogenesis and the expression of the gonadotropins and estrogen isoforms ovarian receptors in the offspring at puberty. At parturition, dams were randomly assigned to the following groups: control (C) group, with free access to a standard laboratory diet containing 23% protein and protein-energy-restricted (PER) group, with free access to an isoenergy and protein-restricted diet containing 8% protein. After weaning, the female pups had free access to standard laboratory diet. The maternal malnutrition caused a significant increase in the number of preantral (C=13.72±2.87; PER=26.36±3.03, P<0.01) and small antral follicles (C=9.32±1.35; PER=17.64±2.33, P<0.01) and decrease in the number of primordial (C=11.72±1.37; PER=3.92±0.60, P<0.01) and Graafian follicles (C=1.84±0.21; PER=0.96±0.11, P<0.01), and corpus luteum (C=2.00±0.28; PER=0.80±0.31, P<0.01). The estradiol serum concentration was significantly higher (C=67.86±4.39; PER=83.29±2.68, P<0.05) while testosterone serum concentration did not show statistical difference (C=0.09±0.02; PER=0.11±0.01, P>0.05) in the PER group. In relation to the receptors expression, maternal malnutrition led to a significant increase in the amount of Fshr (C=0.89±0.04; PER=1.07±0.03, P<0.05) and Lhcqr (C=0.87±0.15; PER=1.33±0.08, P<0.05) transcripts and a significant decrease in the amount of Ar (C=0.59±0.006; PER=0.13±0.080, P<0.05), ERα (Esr1) (C=3.33±0.71; PER=0.74±0.50, P<0.05), ERβ1 (Esr2) (C=1.33±0.06; PER=0.49±0.36, P<0.05), and ERβ2 (Esr2) (C=3.28±0.60; PER=0.62±0.34, P<0.05) transcripts. In conclusion, perinatal maternal malnutrition can directly affect folliculogenesis at puberty probably as a consequence of changes in the ovarian expression of gonadotropins, androgen and estrogens isoforms receptors. Long-term sexual alterations could be expected in this experimental model, since a reduction in the primordial follicle number is observed, which can result in a decrease in the reproductive lifetime and an earlier termination of breeding capacity.

scholarly journals Laboratory Evaluation and Serum Level of Phenobarbital Administered by Different Pathways in Dogs

2018 ◽  
Vol 46 (1) ◽  
pp. 5
Author(s):  
Gabriela Francisco Bittar ◽  
Gleice Laine De Souza ◽  
Gabriela Haro de Melo ◽  
Dayane Aparecida Francisco Da Silva ◽  
José Sérgio Costa Júnior ◽  
...  
Keyword(s):  
Serum Level ◽  
Serum Concentration ◽  
Oral Administration ◽  
Jugular Vein ◽  
Serum Levels ◽  
Serum Biochemistry ◽  
Nasal Administration ◽  
Laboratory Evaluation ◽  
Normal Range ◽  
Significant Difference

Background: Anticonvulsants are widely used in the treatment of small animals for the remission of isolated seizures and recurrent seizures in epilepsy, including tonic-clonic seizures and in status epileticus. Phenobarbital is the drug of choice for the management of epileptic seizures, it is considered very effective, safe, low cost and with few side effects. Several routes of administration may be used, with the oral, intravenous and intramuscular routes being the most common, with rectal and nasal routes being the least common. Materials, Methods & Results: Twenty mongrel dogs were used in the present study (aged 1 to 6-year-old, males and females, weighing 6.0 to 17.0 kg). The patients were previously evaluated via physical examination, temperature, respiratory and heart rate, laboratory tests (erythrogram and leukogram), and serum biochemistry by analyzing the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (FA), and gamma-glutamyltransferase (GGT). Four experimental groups were established with five animals in each group: animals receiving intramuscular injections of phenobarbital (VIM), animals receiving nasal administration of phenobarbital (VN), animals receiving rectal administration of phenobarbital (VR), and animals receiving oral administration of phenobarbital (VO). Phenobarbital was administered every 12 h for 15 days. To determine the serum level of phenobarbital, 5 mL of jugular vein blood was collected in vacuum tubes for evaluation via hemogram. The serum level was determined after 15 days of continuous administration of phenobarbital, as stable phenobarbital serum levels can only be achieved from 10 to 15 days after the first administration. For the serum biochemistry evaluation, 10 mL of blood from the jugular vein was collected using vacuum syringes for assessing ALT, AST, GGT, and FA levels. Statistical analyses were performed with contrasted variation analysis using the Tukey method and paired t-test for comparison of time points. The level of significance was determined to be 5%. There was a significant difference (P < 0.05) among drug administration routes, and among the levels of AST and AF enzymes after the first administration of phenobarbital via the nasal and intramuscular routes. Based on the results, it was concluded that hepatic alterations secondary to the use of phenobarbital for 15 days may occur with a low incidence. The phenobarbital serum concentration in dogs varied when administered via nasal, rectal, and intramuscular routes, but was stable when administered orally.Discussion: The current results are consistent with those of previous studies, indicating that the chronic use of phenobarbital causes an increase in the serum levels of ALT and FA enzymes. It was found that phenobarbital serum levels varied greatly, particularly following nasal, rectal, and intramuscular administration. In the present study, serum phenobarbital levels were within the normal range when administered via the intramuscular, nasal, rectal, and oral routes. This result is inconsistent with that of previous studies where the serum levels were below the normal range following oral administration of phenobarbital. The serum concentration, rather than clinical criteria, should be used as a guide for treatment modification, as the distribution of this drug varies greatly among animals.

scholarly journals Проблемні питання адаптації корів голштинської породи в умовах промислової технології виробництва молока

2017 ◽  
Vol 19 (73) ◽  
pp. 28-32
Author(s):  
R.V. Milostiviy ◽  
О.О. Kalinichenko ◽  
Т.О. Vasilenko ◽  
D.F. Milostivа ◽  
G.S. Gutsulyak
Keyword(s):  
Milk Production ◽  
Production Technology ◽  
First Generation ◽  
Reproductive Organs ◽  
Natural Resistance ◽  
Reproductive Capacity ◽  
Holstein Cattle ◽  
Industrial Complex ◽  
Lactation Period ◽  
Problematic Issue

The increase in the duration of productive longevity of highly productive cows remains a problematic issue and in recent years has attracted increasing attention of domestic and foreign scientists. Accelerated herd turnover and significant premature retirement of Holstein animals in herds may subsequently have negative economic and biological consequences. The analysis of the lifetime productivity of the Holstein cattle of European breeding and their daughters under the conditions of industrial milk production technology has been carried out; the reasons for the culling of animals due to diseases have been clarified. It is established that under the conditions of an industrial complex the period of productive use of Holstein cattle is sharply reduced. The duration of the lactation period in cows-daughters compared with mothers significantly decreased by 463.6 days (by 32.5%) and amounted to only 2.5 lactations (P < 0.01). At the same time, lifelong milk yield was lower by 8427.1 kg (25.9%), and during life from them was received on one calf less. The main reasons for premature departure from the herd of imported animals were: a violation of reproductive capacity (25.6%) and postpartum complications (7.4%), diseases of the digestive system (22.3%), limbs (21.5%) and mastitis (6.6%). Excessive exploitation of animals led to a decrease in natural resistance, which manifested itself in an increase in the number of pathologies of reproductive organs (6.7%), digestive organs (5.5%) and mammary glands (8.5%) among the first-generation cows. Accelerated herd turnover as a result of premature retirement of highly productive cows, necessitates the search for rational ways to increase the natural resistance of the animal organism in conditions of industrial milk production technology aimed at prolonging their productive longevity.

scholarly journals Carcinoembryonic antigen (CEA) as a candidate prognostic marker of nonmucinous pneumonic adenocarcinoma (P-ADC) of the lung.

2019 ◽  
Vol 5 (suppl) ◽  
pp. 28-28
Author(s):  
Satoru Nakamura ◽  
Minoru Fukuda
Keyword(s):  
Serum Level ◽  
Prognostic Marker ◽  
First Generation ◽  
Initial Diagnosis ◽  
Smoking History ◽  
Normal Range ◽  
X Ray ◽  
Chest X Ray ◽  
Serum Cea ◽  
Egfr Tki

28 Background: Serum level of CEA as a prognostic marker in NSCLC is well known. In patients with non-mucinous P-ADC, there were some paradoxical cases with high CEA, in spite of improved symptom and chest X-ray after chemotherapy including first-generation EGFR-TKI. Methods: We retrospectively comfirmed serum level of CEA of five patients, including four patients with non-mucinous P-ADC and one patient with mucinous P-ADC, between 2001-2007, all Japanese female without smoking history, 66-78 years old (mean 73.6 y.o.). All of them revealed abnormal pneumonic shadow on chest X-ray, and were diagnosed by TBLB or cytology. These cases were all stage IIIB or IV, and were applied to chemotherapy including first-generation EGFR-TKI. We had measured serum CEA as possible from initial diagnosis and pre or post chemotherapy including first-generation EGFR-TKI to death. OS were between 25~66 months with four patients of non-mucinous P-ADC, and 9 month with one patient of mucinous P-ADC. Other tumor marker such as CYFRA and Pro GRP were all almost normal range. Results: CEA of three patients with non-mucinous P-ADC was elevated according to worsened symptom and X-ray, and were all highly maintained or increased in spite of improved symptom and X-ray after chemotherapy including first-generation EGFR-TKI. CEA of one patient with non-mucinous P-ADC was normal range in initial diagnosis, and no more measured. She has got symptom free and improved chest X-ray after chemotherapy. In one patient with mucinous P-ADC, serum CEA was normal range, so no longer measured. This patient was no effective for chemotherapy, and was dead 9 month after the initial diagnosis. Number of cases was quite few, so further examination is favorable. Furthermore, All cases were exaggerated finally, they might be resistant to first-generation EGFR-TKI. Conclusions: In three patients with non-mucinous P-ADC of the lung, serum CEA was paradoxically highly maintained or elevated in spite of improved symptom and chest X-ray after chemotherapy including first-generation EGFR-TKI. Therefore, serum CEA is a candidate for useful prognostic marker of non-mucimous P-ADC of the lung.

Insulin Resistance in Adults with Type 1 Diabetes is Associated with Lower Vitamin D Serum Concentration

2019 ◽  
Author(s):  
Mikołaj Kamiński ◽  
Aleksandra Uruska ◽  
Anita Rogowicz-Frontczak ◽  
Dawid Lipski ◽  
Paweł Niedźwiecki ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Vitamin D ◽  
Type 1 Diabetes ◽  
Serum Level ◽  
Serum Concentration ◽  
Test Group ◽  
Glucose Disposal ◽  
Endogenous Insulin ◽  
Estimated Glucose Disposal Rate

Abstract Aim Type 1 diabetes mellitus (T1DM) is a disease characterized by an absolute deficiency of endogenous insulin secretion. Insulin resistance (IR) may develop among patients with T1DM. Vitamin D deficiency was reported to be a risk factor in the development of IR. The aim of the study was to assess the relationship between serum concentrations of 25-hydroxyvitamin D (25(OH)D) and IR among patients with T1DM. Methods The test group consisted of 110 adult patients [males=65 (59%)] with T1DM. Participants were recruited in Poland between 1st October and 30th April in 2015/2016 and 2016/2017. VD serum level was assessed by ELISA array. IR was assessed by estimated Glucose Disposal Rate (eGDR). Results In the study group 21 (19%) patients were recognized as IR according to eGDR cut-offs (<7.5 mg/kg/min), 52 (47.3%) patients had VD deficiency (25(OH)D<20 ng/ml), 16 (14.5%) patients had 25(OH)D<10 ng/ml. Only 6 (5%) participants reported VD supplementation. Patients with IR, according to eGDR cut-off revealed significantly lower 25(OH)D serum level 15.7 (9.2–28.4) vs. 22.1 (13.0–38.4) ng/ml; p=0.04 as compared to patients without IR. R Spearman analysis found a positive relationship between VD and eGDR (Rs=0.27; p<0.01). Logistic regression analysis revealed significant relationship between the presence of IR and VD serum level/presence of 25(OH)D serum level below 10 ng/ml, both models adjusted to sex, age, BMI, LDL and triglycerides, accordingly (OR=0.95, CI: 0.90–0.99; p=0.04) and (OR=4.19, CI: 1.04–16.93; p=0.04). Conclusion The serum concentration of Vitamin D is negatively associated with insulin resistance in patients with T1DM and may have clinical implications.

Serum concentration and properties of α-fetoprotein and serum level of albumin in sucking piglets

1984 ◽  
Vol 36 (2) ◽  
pp. 212-216 ◽  
Author(s):  
T. Fujimoto ◽  
A. Hara ◽  
Y. Maede ◽  
S. Namioka
Keyword(s):  
Serum Level ◽  
Serum Concentration
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