Investigation into antibacterial and wound healing properties of platelets lysate against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections

Abstract Background and aim Treatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections. Methods PdB was prepared through the freezing and thawing process and then, in vitro antibacterial effect was determined by disk diffusion and broth microdilution methods. Afterward, burn wound was inflicted on 56 rats, infected with both bacteria, and topical administration was performed to evaluate antibacterial effects and wound healing properties of PdB. Results In vitro results showed that PdB inhibited the growth of A. baumannii in the highest dose (0.5), while we did not detect any inhibitory effects against K. pneumoniae. By contrast, PdB significantly inhibited the growth of bacteria in treated animal wounds compared to the control groups (P value < 0.05). Macroscopic assessments pointed to the significant enhancement of wound closure in the treated animals. In addition, histopathological examination demonstrated that treatment of rats with PdB led to a considerable increase in re-epithelialization and attenuated the formation of granulation tissue (P value < 0.05). Conclusion The use of topical PdB is an attractive strategy for treating A. baumannii and K. pneumoniae burn wound infections because it inhibits bacterial growth and promotes wound healing properties.

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Antimicrobial and anti-biofilm potencies of dermcidin-derived peptide DCD-1L against Acinetobacter baumannii: an in vivo wound healing model

BMC Microbiology ◽

10.1186/s12866-022-02439-8 ◽

2022 ◽

Vol 22 (1) ◽

Author(s):

Zahra Farshadzadeh ◽

Maryam Pourhajibagher ◽

Behrouz Taheri ◽

Alireza Ekrami ◽

Mohammad Hossein Modarressi ◽

...

Keyword(s):

Wound Healing ◽

Acinetobacter Baumannii ◽

Biofilm Formation ◽

Histopathological Examination ◽

Inhibitory Effect ◽

Bacterial Attachment ◽

Burn Wound ◽

Conventional Antibiotics

Abstract Background The global emergence of Acinetobacter baumannii resistance to most conventional antibiotics presents a major therapeutic challenge and necessitates the discovery of new antibacterial agents. The purpose of this study was to investigate in vitro and in vivo anti-biofilm potency of dermcidin-1L (DCD-1L) against extensively drug-resistant (XDR)-, pandrug-resistant (PDR)-, and ATCC19606-A. baumannii. Methods After determination of minimum inhibitory concentration (MIC) of DCD-1L, in vitro anti-adhesive and anti-biofilm activities of DCD-1L were evaluated. Cytotoxicity, hemolytic activity, and the effect of DCD-1L treatment on the expression of various biofilm-associated genes were determined. The inhibitory effect of DCD-1L on biofilm formation in the model of catheter-associated infection, as well as, histopathological examination of the burn wound sites of mice treated with DCD-1L were assessed. Results The bacterial adhesion and biofilm formation in all A. baumannii isolates were inhibited at 2 × , 4 × , and 8 × MIC of DCD-1L, while only 8 × MIC of DCD-1L was able to destroy the pre-formed biofilm in vitro. Also, reduce the expression of genes involved in biofilm formation was observed following DCD-1L treatment. DCD-1L without cytotoxic and hemolytic activities significantly reduced the biofilm formation in the model of catheter-associated infection. In vivo results showed that the count of A. baumannii in infected wounds was significantly decreased and the promotion in wound healing by the acceleration of skin re-epithelialization in mice was observed following treatment with 8 × MIC of DCD-1L. Conclusions Results of this study demonstrated that DCD-1L can inhibit bacterial attachment and biofilm formation and prevent the onset of infection. Taking these properties together, DCD-1L appears as a promising candidate for antimicrobial and anti-biofilm drug development.

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Photoelimination Potential of Chitosan NanoparticlesIndocyanine Green Complex Against the Biological Activities of Acinetobacter baumannii Strains: A Preliminary In Vitro Study in Burn Wound Infections

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.31 ◽

2020 ◽

Vol 11 (2) ◽

pp. 187-192 ◽

Cited By ~ 1

Author(s):

Maryam Pourhajibagher ◽

Nava Hosseini ◽

Ebrahim Boluki ◽

Nasim Chiniforush ◽

Abbas Bahador

Keyword(s):

Acinetobacter Baumannii ◽

Diode Laser ◽

Biological Activities ◽

Chitosan Nanoparticles ◽

Control Group ◽

Wound Infections ◽

Burn Wound ◽

Sem Images ◽

Burn Wounds

Introduction: Acinetobacter baumannii strains are important agents causing serious nosocomial infections including soft-tissue and skin infections in patients with burn wounds which have become resistant to several classes of antibiotics. Antimicrobial photodynamic therapy (aPDT) as an alternative antimicrobial procedure is suggested for the treatment of these kinds of infections. The aim of the current study is to evaluate the antibacterial and anti-biofilm efficiency of aPDT by the utilization of an improved form of indocyanine green (ICG) which is encapsulated in chitosan nanoparticles (NCs@ICG). Methods: NCs@ICG were synthesized and confirmed by the scanning electron microscope (SEM). aPDT was performed using NCs@ICG with an 810 nm wavelength of the diode laser at the fluency of 31.2 J/cm2 on 50 A. baumannii strains isolated from burn wounds. The antibacterial and antibiofilm potential of NCs@ICG-aPDT was determined via the colony forming unit (CFU)/mL and crystal violet assays, respectively. In addition, microbial biofilm degradation was evaluated by the SEM. Results: According to the results, NCs@ICG-aPDT showed a significant reduction of 93.2% on the CFU/ mL of planktonic A. baumannii strains compared to the control group (untreated group; P < 0.05). In addition, the biofilm formation of A. baumannii strains was significantly reduced by 55.3% when the bacteria were exposed to NCs@ICG-aPDT (P < 0.05). In contrast, NCs@ICG, ICG, and the diode laser alone were not able to inhibit the CFU/mL and biofilm of A. baumannii strains (P > 0.05). Based on the results of SEM images, NCs@ICG-aPDT disrupted the biofilm structure of A. baumannii strains more than other groups. Conclusion: NCs@ICG-aPDT demonstrates a promising treatment candidate for exploitation in wound infections against both planktonic and biofilm forms of A. baumannii strains

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Development of Cephradine-Loaded Gelatin/Polyvinyl Alcohol Electrospun Nanofibers for Effective Diabetic Wound Healing: In-Vitro and In-Vivo Assessments

Pharmaceutics ◽

10.3390/pharmaceutics13030349 ◽

2021 ◽

Vol 13 (3) ◽

pp. 349

Author(s):

Anam Razzaq ◽

Zaheer Ullah Khan ◽

Aasim Saeed ◽

Kiramat Ali Shah ◽

Naveed Ullah Khan ◽

...

Keyword(s):

Wound Healing ◽

Polyvinyl Alcohol ◽

Mtt Assay ◽

Chronic Wound ◽

Drug Loading ◽

Group Identification ◽

Wound Infections ◽

Diabetic Wound

Diabetic wound infections caused by conventional antibiotic-resistant Staphylococcus aureus strains are fast emerging, leading to life-threatening situations (e.g., high costs, morbidity, and mortality) associated with delayed healing and chronic inflammation. Electrospinning is one of the most widely used techniques for the fabrication of nanofibers (NFs), induced by a high voltage applied to a drug-loaded polymer solution. Particular attention is given to electrospun NFs for pharmaceutical applications (e.g., original drug delivery systems) and tissue regeneration (e.g., as tissue scaffolds). However, there is a paucity of reports related to their application in diabetic wound infections. Therefore, we prepared eco-friendly, biodegradable, low-immunogenic, and biocompatible gelatin (GEL)/polyvinyl alcohol (PVA) electrospun NFs (BNFs), in which we loaded the broad-spectrum antibiotic cephradine (Ceph). The resulting drug-loaded NFs (LNFs) were characterized physically using ultraviolet-visible (UV-Vis) spectrophotometry (for drug loading capacity (LC), drug encapsulation efficiency (EE), and drug release kinetics determination), thermogravimetric analysis (TGA) (for thermostability evaluation), scanning electron microscopy (SEM) (for surface morphology analysis), and Fourier-transform infrared spectroscopy (FTIR) (for functional group identification). LNFs were further characterized biologically by in-vitro assessment of their potency against S. aureus clinical strains (N = 16) using the Kirby–Bauer test and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, by ex-vivo assessment to evaluate their cytotoxicity against primary human epidermal keratinocytes using MTT assay, and by in-vivo assessment to estimate their diabetic chronic wound-healing efficiency using NcZ10 diabetic/obese mice (N = 18). Thin and uniform NFs with a smooth surface and standard size (<400 nm) were observed by SEM at the optimized 5:5 (GEL:PVA) volumetric ratio. FTIR analyses confirmed the drug loading into BNFs. Compared to free Ceph, LNFs were significantly more thermostable and exhibited sustained/controlled Ceph release. LNFs also exerted a significantly stronger antibacterial activity both in-vitro and in-vivo. LNFs were significantly safer and more efficient for bacterial clearance-induced faster chronic wound healing. LNF-based therapy could be employed as a valuable dressing material to heal S. aureus-induced chronic wounds in diabetic subjects.

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Extended Spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from Burn Wound Infections

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2016.509.043 ◽

2016 ◽

Vol 5 (9) ◽

pp. 400-404 ◽

Cited By ~ 1

Author(s):

Khulood Abdulkareen Hussein ◽

Zainab Alag Hasan ◽

Utoor Talib Jassim ◽

Abdulameer Abdullah Al-Mussawi

Keyword(s):

Klebsiella Pneumoniae ◽

Wound Infections ◽

Burn Wound ◽

Extended Spectrum

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A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽

10.3390/biomedicines9091153 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1153

Author(s):

Verena Schneider ◽

Daniel Kruse ◽

Ives Bernardelli de Mattos ◽

Saskia Zöphel ◽

Kendra-Kathrin Tiltmann ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Healing Process ◽

Three Dimensional ◽

Source Model ◽

Burn Wound ◽

Thermal Burn ◽

Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

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Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

10.21203/rs.3.rs-19542/v1 ◽

2020 ◽

Author(s):

Lei Yang ◽

Qing Huang ◽

Xingxin Gao ◽

Zhimin Lin ◽

Songlin Chen ◽

...

Keyword(s):

Volatile Oil ◽

Bacterial Biofilm ◽

Control Group ◽

Wound Infections ◽

Eucalyptus Urophylla ◽

Burn Wound ◽

Working Mechanism ◽

Biofilm Model ◽

Prevention And Treatment

Abstract Objective To investigate working mechanism of Eucalyptus volatile oil on the prevention and treatment of burn wound infections. Methods P.a biofilm model was used to investigate the effect of Eucalyptus volatile oil on bacterial biofilm. The expression of LasI mRNA in P.a was detected by RT-PCR. Results MIC test showed that the volatile oil of Eucalyptus Urophylla in a concentration of 20% or more could exert anti-bacterial effect. However, no zone of inhibition could be observed in the neither high nor low concentration of the Eucalyptus volatile oil. Scanning electron microscopy results showed a significant delay in volatile oil groups when compared with the control group. The expression of LasI mRNA in the volatile oil group was significantly lower than that in the control group. Conclusion As Chinese medicine, the volatile oil of Eucalyptus Urophylla can affect the proliferation of P.a and biofilm formation by interfering with the expression of LasI, thus achieving the purpose of preventing and treating infection of burn patients.

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A comparative study of the effect of different topical agents on burn wound infections

Indian Journal of Plastic Surgery ◽

10.4103/0970-0358.101320 ◽

2012 ◽

Vol 45 (02) ◽

pp. 374-378 ◽

Cited By ~ 8

Author(s):

Katara Gunjan ◽

Chamania Shobha ◽

Chitnis Sheetal ◽

Hemvani Nanda ◽

Chitnis Vikrant ◽

...

Keyword(s):

Silver Nitrate ◽

Benzalkonium Chloride ◽

Research Centre ◽

Topical Agent ◽

Wound Infections ◽

Burn Wound ◽

Gram Positive ◽

Gram Negative ◽

Excellent Activity

ABSTRACT Background: Topical agents are used to treat burn wound infections. Aims and Objective: The present work was aimed to find out the in vitro efficacy of different topical agents against burn wound pathogens. Settings and Design: Randomly selected gram-positive (29) and gram-negative bacterial (119) isolates from burn wound cases admitted in burn unit of Choithram Hospital and Research Centre, Indore, were included in the in vitro activity testing for silver nitrate, silver sulphadiazine (SSD), chlorhexidine, cetrimide, nitrofuran, soframycin, betadine, benzalkonium chloride and honey by growth inhibition on agar medium. Materials and Methods: Multidrug-resistant isolates of gram-positive and gram-negative bacteria were checked for different topical agents. 1% topical agent was mixed with Mueller-Hinton agar. Two microlitres of bacterial suspension adjusted to 0.5 McFarland turbidity standard was spread over the topical agent containing plates. The plates without the topical agent were used as control plates. The plates were incubated for 48 h at 37΀C. Results: SSD (148/148), silver nitrate (148/148) and chlorhexidine (148/148) showed excellent activity against all the pathogens. Neosporin had poor activity against Pseudomonas aeruginosa, (4/44) Proteus spp. (2/4) and group D streptococci (1/4). Betadine did not show activity against the bacterial isolates in the presence of organic matter. Honey did not exert any antimicrobial activity under the study conditions. Conclusion: SSD, silver nitrate and chlorhexidine have excellent activity against all the bacterial pathogens and could be used empirically, while identification of the infective agent is required for selecting the alternative topical agents such as nitrofuran, soframycin, and benzalkonium chloride.

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Synergistic Activity of Colistin/Fosfomycin Combination against Carbapenemase-Producing Klebsiella pneumoniae in an In Vitro Pharmacokinetic/Pharmacodynamic Model

BioMed Research International ◽

10.1155/2018/5720417 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Jin Wang ◽

Ji-tong He ◽

Yan Bai ◽

Rui Wang ◽

Yun Cai

Keyword(s):

Klebsiella Pneumoniae ◽

Bactericidal Effect ◽

Combination Regimen ◽

Synergistic Activity ◽

Antibacterial Effects ◽

Killing Effect ◽

Initial Inoculum ◽

Susceptible Isolate ◽

Combination Regimens

Carbapenemase-producing Klebsiella pneumoniae is globally recognized as one of the greatest threats to public health, and combination therapy may be the chemotherapeutic option. In the present study, we aimed to evaluate the antibacterial effects of colistin/fosfomycin combination against carbapenemase-producing K. pneumoniae. The antibacterial effects were determined in a one-compartment in vitro pharmacokinetic model over a period of 24 h. The initial inoculum was 108 CFU/mL. Low, medium, and high Cmax values of colistin at 0.5, 2, and 5 mg/L as well as Cmax of fosfomycin at 100 mg/L were simulated in the model. Doses of both colistin and fosfomycin were given every 8 h until 24 h. For the colistin- and fosfomycin-susceptible isolate KP47, three combination regimens showed greater killing effect compared with colistin monotherapy. The greatest killing effect was observed in combination regimen containing 5 mg/L colistin. For colistin-heteroresistant and fosfomycin-susceptible isolate KP79, combination regimen containing low dose colistin (0.5 mg/L) showed no synergistic or additive effects. However, combination regimens containing 2 and 5 mg/L colistin maintained the bactericidal effect until 24 h compared with colistin monotherapy. For colistin-heteroresistant and fosfomycin-resistant isolates KP42 and KP11, bactericidal activity was barely enhanced by combination regimens. Moreover, combination regimen containing 5 mg/L colistin could only prevent the emergence of colistin-resistant subpopulation in colistin and fosfomycin-susceptible isolate. It is necessary to know the resistant patterns of the K. pneumoniae before using combination of colistin and fosfomycin in clinical practice.

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Injectable Nanocurcumin-Formulated Chitosan-g-Pluronic Hydrogel Exhibiting a Great Potential for Burn Treatment

Journal of Healthcare Engineering ◽

10.1155/2018/5754890 ◽

2018 ◽

Vol 2018 ◽

pp. 1-14 ◽

Cited By ~ 18

Author(s):

Le Hang Dang ◽

Thi Hiep Nguyen ◽

Ha Le Bao Tran ◽

Vu Nguyen Doan ◽

Ngoc Quyen Tran

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Healing Process ◽

Nanocomposite Hydrogel ◽

Burn Wound ◽

Burn Treatment ◽

Nanocomposite Hydrogels ◽

Burn Wound Healing

Burn wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent; therefore, it can prevent the prolonged presence of oxygen free radicals which is a significant factor causing inhabitation of optimum healing process. This study describes an extension of study about the biofunctional nanocomposite hydrogel platform that was prepared by using curcumin and an amphiphilic chitosan-g-pluronic copolymer specialized in burn wound healing application. This formular (nCur-CP, nanocomposite hydrogel) was a free-flowing sol at ambient temperature and instantly converted into a nonflowing gel at body temperature. In addition, the storage study determined the great stability level of nCur-CP in long time using UV-Vis and DLS. Morphology and distribution of nCur in its nanocomposite hydrogels were observed by SEM and TEM, respectively. In vitro studies suggested that nCur-CP exhibited well fibroblast proliferation and ability in antimicrobacteria. Furthermore, second- and third-degree burn wound models were employed to evaluate the in vivo wound healing activity of the nCur-CP. In the second-degree wound model, the nanocomposite hydrogel group showed a higher regenerated collagen density and thicker epidermis layer formation. In third degree, the nCur-CP group also exhibited enhancement of wound closure. Besides, in both models, the nanocomposite material-treated groups showed higher collagen content, better granulation, and higher wound maturity. Histopathologic examination also implied that the nanocomposite hydrogel based on nanocurcumin and chitosan could enhance burn wound repair. In conclusion, the biocompatible and injectable nanocomposite scaffold might have great potential to apply for wound healing.

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An Integrated HOCl-Producing E-Scaffold Is Active Against Monomicrobial and Polymicrobial Biofilms

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02007-20 ◽

2021 ◽

Author(s):

Laure Flurin ◽

Yash S. Raval ◽

Abdelrhman Mohamed ◽

Kerryl E. Greenwood-Quaintance ◽

Edison J. Cano ◽

...

Keyword(s):

Low Dose ◽

Hypochlorous Acid ◽

Viable Cell ◽

Chloride Ions ◽

Resistant Bacteria ◽

Wound Infections ◽

Microbial Biofilms ◽

Cell Counts ◽

Acquired Antibiotic Resistance

Oxidizing agents like hypochlorous acid (HOCl) have antimicrobial activity. We developed an integrated electrochemical scaffold or ‘e-scaffold’ that delivers a continuous low dose of HOCl aimed at targeting microbial biofilms without exceeding concentrations toxic to humans, as a prototype of a device being developed to treat wound infections in humans. In this work, we tested the device against 33 isolates of bacteria (including isolates with acquired antibiotic resistance) grown as in vitro biofilms, alongside 12 combinations of dual-species in vitro biofilms. Biofilms were grown on the bottoms of 12-well plates for 24 hours. An integrated e-scaffold was placed atop each biofilm and polarized at 1.5V for 1, 2 or 4 hours. HOCl was produced electrochemically by oxidizing chloride ions (Cl-) in solution to chlorine (Cl2); dissolved Cl2 spontaneously dissociates in water to produce HOCl. The cumulative concentration of HOCl produced at the working electrode in each well was estimated to be 7.89, 13.46, and 29.50 mM after 1, 2 and 4 hours of polarization, respectively. Four hours of polarization caused an average reduction of 6.13 log10 CFU/cm2 (±1.99 log10 CFU/cm2) of viable cell counts of mono-species biofilms and 5.53 log10 CFU/cm2 (±2.31 log10 CFU/cm2) for the 12 dual-species biofilms studied. The described integrated e-scaffold reduces viable bacterial cell counts in biofilms formed by an array of antibiotic-susceptible and -resistant bacteria alone and in combination.

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