pH-dependent nanodiamonds enhance the mechanical properties of 3D-printed hyaluronic acid nanocomposite hydrogels

Abstract Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality. 3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt % resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites. These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner. HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40 fold (0.02%: 236.18 kPa) and 1.37 fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt % and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt %) at pH 8 was mechanically enhanced 1.29 fold, compared to that of HA/ND-OH (0.04 wt %) at pH 7. These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel. Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.

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pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

10.21203/rs.3.rs-18085/v1 ◽

2020 ◽

Author(s):

Dae Gon Lim ◽

Eunah Kang ◽

Seong Hoon Jeong

Keyword(s):

Mechanical Properties ◽

Hyaluronic Acid ◽

3D Printing ◽

Compressive Stress ◽

Critical Role ◽

Biological Tissues ◽

Dependent Manner ◽

Inorganic Particles ◽

Nanocomposite Hydrogels ◽

Ph Dependent

Abstract Background: Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality. Mechanically enhanced biomaterials composited with diverse nanophase inorganic particles are challenging because metastatic materials mimicking biological tissues require softness, biocompatibility, strength, and structurally compatible elasticity. Herein, nanodiamonds (NDs) have been chosen due to their hydrophilicity and pH-dependent surface functionality, along with their convenient chemical modalityResults: 3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt % resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites. These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner. HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40 fold (236.18 kPa) and 1.37 fold (616.72 kPa) the compressive stress at the composition of 0.02 wt % and 0.04 wt %, respectively, compared to those of ND-COOH (168.31, 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt %) at pH 8 was mechanically enhanced 1.29 fold, compared to that of HA/ND-OH (0.04 wt %) at pH 7. These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Conclusion: Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel. Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.

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Dual-Crosslinking of Hyaluronic Acid-Calcium Phosphate Nanocomposite Hydrogels for Enhanced Mechanical Properties and Biological Performance

Macromolecular Materials and Engineering ◽

10.1002/mame.201700160 ◽

2017 ◽

Vol 302 (10) ◽

pp. 1700160 ◽

Cited By ~ 5

Author(s):

Seol-Ha Jeong ◽

Jeong-Yun Sun ◽

Hyoun-Ee Kim

Keyword(s):

Mechanical Properties ◽

Hyaluronic Acid ◽

Calcium Phosphate ◽

Nanocomposite Hydrogels ◽

Biological Performance

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Effect of Delay Time on the Mechanical Properties of Extrusion-Based 3D Printed Concrete

Proceedings of the 34th International Symposium on Automation and Robotics in Construction (ISARC) ◽

10.22260/isarc2017/0032 ◽

2017 ◽

Cited By ~ 7

Author(s):

Taylor Marchment ◽

Ming Xia ◽

Elise Dodd ◽

Jay Sanjayan ◽

Behzad Nematollahi

Keyword(s):

Mechanical Properties ◽

Delay Time ◽

3D Printed

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Influence of slicing parameters on surface quality and mechanical properties of 3D-printed CF/PLA composites fabricated by FDM technique

Materials Technology ◽

10.1080/10667857.2021.1915056 ◽

2021 ◽

pp. 1-18

Author(s):

N. Vinoth Babu ◽

N. Venkateshwaran ◽

N. Rajini ◽

Sikiru Oluwarotimi Ismail ◽

Faruq Mohammad ◽

...

Keyword(s):

Mechanical Properties ◽

Surface Quality ◽

3D Printed

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Effects of sandwich core structure and infill rate on mechanical properties of 3D-printed wood/PLA composites

The International Journal of Advanced Manufacturing Technology ◽

10.1007/s00170-021-07382-y ◽

2021 ◽

Author(s):

Nadir Ayrilmis ◽

Mirko Kariz ◽

Milan Šernek ◽

Manja Kitek Kuzman

Keyword(s):

Mechanical Properties ◽

Core Structure ◽

3D Printed ◽

Sandwich Core

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Fabrication of Polycaprolactone/Nano Hydroxyapatite (PCL/nHA) 3D Scaffold with Enhanced In Vitro Cell Response via Design for Additive Manufacturing (DfAM)

Polymers ◽

10.3390/polym13091394 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1394

Author(s):

Yong Sang Cho ◽

So-Jung Gwak ◽

Young-Sam Cho

Keyword(s):

Mechanical Properties ◽

Cell Response ◽

Structural Characteristics ◽

Structure Design ◽

Compressive Modulus ◽

Control Group ◽

Design For Additive Manufacturing ◽

3D Scaffold ◽

3D Printed

In this study, we investigated the dual-pore kagome-structure design of a 3D-printed scaffold with enhanced in vitro cell response and compared the mechanical properties with 3D-printed scaffolds with conventional or offset patterns. The compressive modulus of the 3D-printed scaffold with the proposed design was found to resemble that of the 3D-printed scaffold with a conventional pattern at similar pore sizes despite higher porosity. Furthermore, the compressive modulus of the proposed scaffold surpassed that of the 3D-printed scaffold with conventional and offset patterns at similar porosities owing to the structural characteristics of the kagome structure. Regarding the in vitro cell response, cell adhesion, cell growth, and ALP concentration of the proposed scaffold for 14 days was superior to those of the control group scaffolds. Consequently, we found that the mechanical properties and in vitro cell response of the 3D-printed scaffold could be improved by kagome and dual-pore structures through DfAM. Moreover, we revealed that the dual-pore structure is effective for the in vitro cell response compared to the structures possessing conventional and offset patterns.

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Physical property of 3D-printed sinusoidal pattern using shape memory TPU filament

Textile Research Journal ◽

10.1177/0040517520919750 ◽

2020 ◽

Vol 90 (21-22) ◽

pp. 2399-2410 ◽

Cited By ~ 2

Author(s):

Shahbaj Kabir ◽

Hyelim Kim ◽

Sunhee Lee

Keyword(s):

Mechanical Properties ◽

Tensile Test ◽

Shape Memory ◽

Fused Deposition Modeling ◽

Loss Modulus ◽

Thermoplastic Polyurethane ◽

Heating Process ◽

Fused Deposition ◽

3D Printed ◽

Sinusoidal Pattern

This study has investigated the physical properties of 3D-printable shape memory thermoplastic polyurethane (SMTPU) filament and its 3D-printed sinusoidal pattern obtained by fused deposition modeling (FDM) technology. To investigate 3D filaments, thermoplastic polyurethane (TPU) and SMTPU filament were examined by conducting infrared spectroscopy, x-ray diffraction (XRD), dynamic mechanical thermal analysis (DMTA), differential scanning calorimetry (DSC) and a tensile test. Then, to examine the 3D-printed sinusoidal samples, a sinusoidal pattern was developed and 3D-printed. Those samples went through a three-step heating process: (a) untreated state; (b) 5 min heating at 70°C, cooling for 30 min at room temperature; and (c) a repeat of step 2. The results obtained by the three different heating processes of the 3D-printed sinusoidal samples were examined by XRD, DMTA, DSC and the tensile test to obtain the effect of heating or annealing on the structural and mechanical properties. The results show significant changes in structure, crystallinity and thermal and mechanical properties of SMTPU 3D-printed samples due to the heating steps. XRD showed the increase in crystallinity with heating. In DMTA, storage modulus, loss modulus and the tan σ peak position also changed for various heating steps. The DSC result showed that the Tg for different steps of the SMTPU 3D-printed sample remained almost the same at around 51°C. The tensile property of the TPU 3D-printed sinusoidal sample decreased in terms of both load and elongation with increased heating processes, while for the SMTPU 3D-printed sinusoidal sample, the load decreased but elongation increased about 2.5 times.

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