scholarly journals Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Juan Huang ◽  
Ziyan Yang ◽  
Yanyun Li ◽  
Xingxing Chai ◽  
Yanfang Liang ◽  
...  
Keyword(s):  
Treg Cell ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Lactobacillus Paracasei ◽  
Intestinal Microbiome ◽  
Host Responses ◽  
Murine Colitis ◽  
Bowel Diseases ◽  
Infant Feces ◽  
Cell Balance

AbstractInflammatory bowel diseases (IBD), mainly comprising ulcerative colitis (UC) and Crohn's Disease, are most often a polygenic disorder with contributions from the intestinal microbiome, defects in barrier function, and dysregulated host responses to microbial stimulation. Strategies that target the microbiota have emerged as potential therapies and, of these, probiotics have gained the greatest attention. Herein, we isolated a strain of Lactobacillus paracasei R3 (L.p R3) with strong biofilm formation ability from infant feces. Interestingly, we also found L.p R3 strain can ameliorate the general symptoms of murine colitis, alleviate inflammatory cell infiltration and inhibit Th17 while promote Treg function in murine dextran sulfate sodium (DSS)-induced colitis. Overall, this study suggested that L.p R3 strain significantly improves the symptoms and the pathological damage of mice with colitis and influences the immune function by regulating Th17/Treg cell balance in DSS-induced colitis in mice.

scholarly journals Lactobacillus Paracasei R3 Protects Against Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice via Regulating Th17/Treg Cell Balance

2021 ◽  
Author(s):  
Juan Huang ◽  
Ziyan Yang ◽  
Yanyun Li ◽  
Xingxing Chai ◽  
Yanfang Liang ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Lactobacillus Paracasei ◽  
Intestinal Microbiome ◽  
Host Responses ◽  
Murine Colitis ◽  
Treg Function ◽  
Bowel Diseases ◽  
Infant Feces ◽  
Inflammatory Bowel ◽  
Cell Balance

Abstract Inflammatory bowel diseases (IBD), mainly comprising ulcerative colitis (UC) and Crohn's Disease, are most often a polygenic disorder with contributions from the intestinal microbiome, defects in barrier function, and dysregulated host responses to microbial stimulation. Strategies that target the microbiota have emerged as potential therapies and, of these, probiotics have gained the greatest attention. Herein, we isolated a strain of Lactobacillus paracasei R3 (L.p R3) with strong biofilm formation ability from infant feces. Interestingly, we also found L.p R3 strain can ameliorate the general symptoms of murine colitis, alleviate inflammatory cell infiltration and inhibit Th17 while promote Treg function in murine DSS-induced colitis. Overall, this study suggested that L.p R3 strain significantly improves the symptoms and the pathological damage of mice with colitis and influences the immune function by regulating Th17/Treg cell balance in DSS-induced colitis in mice.

scholarly journals Heme Oxygenase-1 Ameliorates Dextran Sulfate Sodium-induced Acute Murine Colitis by Regulating Th17/Treg Cell Balance

2014 ◽  
Vol 289 (39) ◽  
pp. 26847-26858 ◽  
Author(s):  
Liya Zhang ◽  
Yanjie Zhang ◽  
Wenwei Zhong ◽  
Caixia Di ◽  
Xiaoliang Lin ◽  
...  
Keyword(s):  
Treg Cell ◽  
Heme Oxygenase ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Heme Oxygenase 1 ◽  
Murine Colitis ◽  
Cell Balance

scholarly journals Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance

2021 ◽  
Vol 12 ◽  
Author(s):  
Yaohua Fan ◽  
Yanqun Fan ◽  
Kunfeng Liu ◽  
Piyanuch Lonan ◽  
Feng Liao ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Protective Effect ◽  
Treg Cell ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
The Body ◽  
Inflammatory Factor ◽  
Neuroprotective Effects ◽  
Edible Bird’S Nest ◽  
Cell Balance

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) with a complex aetiology that commonly recurs. Most drugs for UC treatment interfere with metabolism and immune responses, often causing some serious adverse reactions. Therefore, the development of alternative treatments, including nutritional supplements and probiotics, have been one of the main areas of current research due to fewer side effect. As both a Chinese medicine and a food, edible bird’s nest (EBN) has high nutritional value. Modern pharmacological studies have shown that it has anti-inflammatory, immunoregulatory, antiviral and neuroprotective effects. In this study, UC was induced with dextran sulfate sodium (DSS) to investigate the protective effect of EBN on colitis mice and the related mechanism. The body weight, faecal morphology and faecal occult blood results of mice were recorded every day from the beginning of the modelling period. After the end of the experiment, the length of the colon was measured, and the colon was collected for histopathological detection, inflammatory factor detection and immunohistochemical detection. Mouse spleens were dissected for flow cytometry. The results showed that in mice with colitis, EBN improved symptoms of colitis, reduced colonic injury, and inhibited the increases in the levels of the pro-inflammatory cytokines IL-1β and TNF-α. The T helper 17 (Th17)/regulatory T (Treg) cell balance was restored by decreasing the expression of IL-17A and IL-6 in intestinal tissues, increasing the expression of TGF-β, and decreasing the number of Th17 cells in each EBN dose group. These findings suggest that EBN has a protective effect on DSS-mediated colitis in mice, mainly by restoring the Th17/Treg cell balance.

Complement component 6 deficiency increases susceptibility to dextran sulfate sodium-induced murine colitis

Immunobiology ◽  
2016 ◽  
Vol 221 (11) ◽  
pp. 1293-1303 ◽  
Author(s):  
Peipei Ding ◽  
Ling Li ◽  
Tianbao Huang ◽  
Chaoqun Yang ◽  
Enjie Xu ◽  
...  
Keyword(s):  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Complement Component ◽  
Murine Colitis

scholarly journals Treatment of Dextran Sulfate Sodium-Induced Colitis with Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Inhibitor MI-2 Is Associated with Restoration of Gut Immune Function and the Microbiota

2018 ◽  
Vol 86 (12) ◽  
Author(s):  
Kyung Won Lee ◽  
Minseok Kim ◽  
Chang Hoon Lee
Keyword(s):  
Ulcerative Colitis ◽  
Lymphoid Tissue ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Inflammatory Responses ◽  
Fecal Microbiota ◽  
Intestinal Microbiome ◽  
Diverse Populations ◽  
Necrosis Factor Alpha ◽  
Intestinal Immune System

ABSTRACT Disruption of the healthy intestinal microbiome and homeostasis of the intestinal immune system, which are closely interactive, are two key factors for ulcerative colitis. Here, we show that MI-2, a selective inhibitor of mucosa-associated lymphoid tissue lymphoma translocation-1 (MALT1), alleviated excessive inflammatory responses and was associated with restoration of healthy intestinal microbiome in mice suffering from dextran sulfate sodium (DSS)-induced colitis. We found that the diversity of intestinal microbiome of mice with DSS-induced colitis was significantly lower than that of healthy mice. However, MI-2 treatment in mice with DSS-induced colitis resulted in restored microbially diverse populations. To understand the possibility of the beneficial effect of the restored microbially diverse populations of MI-2-treated mice with DSS-induced colitis, we showed that inserting fecal microbiota from MI-2-treated mice with DSS-induced colitis and healthy control mice into mice with DSS-induced colitis could alleviate symptoms of colitis. The possibility of MI-2 treatment in DSS-induced colitis, associated with restoration of healthy microbially diverse populations in addition to reshaping host immune modulating capacity by reducing inflammatory cytokines (tumor necrosis factor alpha, interleukin-1β [IL-1β], IL-17α, and IL-22), may be considered therapeutic for ulcerative colitis.

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