Integrated data analysis reveals potential drivers and pathways disrupted by DNA methylation in papillary thyroid carcinomas

Background: The clinical pathological features, as well as the cellular mechanisms of miR-195, have not been investigated in thyroid carcinoma. Objective: The aim of this study is to identify the interactions of vascular endothelial growth factor (VEGF), p53 and miR-195 in thyroid carcinoma. The clinical and pathological features of miR-195 were also investigated. Methods: The expression levels of miR-195 were identified in 123 primary thyroid carcinomas, 40 lymph nodes with metastatic papillary thyroid carcinomas and seven non-neoplastic thyroid tissues (controls) as well as two thyroid carcinoma cell lines, B-CPAP (from metastasizing human papillary thyroid carcinoma) and MB-1 (from anaplastic thyroid carcinoma), by the real-time polymerase chain reaction. Using Western blot and immunofluorescence, the effects of exogenous miR-195 on VEGF-A and p53 protein expression levels were examined. Then, cell cycle and apoptosis assays were performed to evaluate the roles of miR-195 in cell cycle progression and apoptosis. Results: The expression of miR-195 was downregulated in majority of the papillary thyroid carcinoma tissue as well as in cells. Introduction of exogenous miR-195 resulted in downregulation of VEGF-A and upregulation of p53 protein expressions. Upregulation of miR-195 in thyroid carcinoma cells resulted in cell cycle arrest. Moreover, we demonstrated that miR-195 inhibits cell cycle progression by induction of apoptosis in the thyroid carcinoma cells. Conclusion: Our findings showed for the first time that miR-195 acts as a tumour suppressor and regulates cell cycle progression and apoptosis by targeting VEGF-A and p53 in thyroid carcinoma. The current study exhibited that miR-195 might represent a potential therapeutic target for patients with thyroid carcinomas having aggressive clinical behaviour.

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Preoperative detection of the TERT promoter mutations in papillary thyroid carcinomas

Clinical Endocrinology ◽

10.1111/cen.14567 ◽

2021 ◽

Author(s):

Tomoe Nakao ◽

Michiko Matsuse ◽

Vladimir Saenko ◽

Tatiana Rogounovitch ◽

Aya Tanaka ◽

...

Keyword(s):

Papillary Thyroid ◽

Thyroid Carcinomas ◽

Tert Promoter ◽

Tert Promoter Mutations ◽

Promoter Mutations

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Heterogenous nature of gene expression patterns in BRAF-like papillary thyroid carcinomas with BRAFV600E

Endocrine ◽

10.1007/s12020-019-02063-z ◽

2019 ◽

Vol 66 (3) ◽

pp. 607-613

Author(s):

Yuji Nagayama ◽

Hiroyuki Mishima

Keyword(s):

Gene Expression ◽

Expression Patterns ◽

Gene Expression Patterns ◽

Papillary Thyroid ◽

Thyroid Carcinomas

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A Model of Pore Pressure and Effective Stress Changes During Hydrocarbon Depletion by Slowness Integrated Data Analysis

On Significant Applications of Geophysical Methods - Advances in Science, Technology & Innovation ◽

10.1007/978-3-030-01656-2_36 ◽

2019 ◽

pp. 161-163 ◽

Cited By ~ 1

Author(s):

Kurniawan Adha ◽

Wan Ismail Wan Yusoff ◽

Luluan Almanna Lubis

Keyword(s):

Data Analysis ◽

Pore Pressure ◽

Effective Stress ◽

Stress Changes ◽

Integrated Data Analysis

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Assessment of Biomarkers for Clinical Diagnosis of Papillary Thyroid Carcinoma with Distant Metastasis

The International Journal of Biological Markers ◽

10.1177/172460081002500106 ◽

2010 ◽

Vol 25 (1) ◽

pp. 38-45 ◽

Cited By ~ 20

Author(s):

Huasheng Liang ◽

Yuhua Zhong ◽

Zuojie Luo ◽

Yu Huang ◽

Huade Lin ◽

...

Keyword(s):

Growth Factor ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Clinical Diagnosis ◽

Distant Metastasis ◽

Roc Curve ◽

Curve Analysis ◽

Papillary Thyroid ◽

Roc Curve Analysis ◽

Thyroid Carcinomas

Early diagnosis and treatment of thyroid cancers are critical for better prognosis and better survival rates. The purpose of this study was to identify potential diagnostic markers for papillary thyroid carcinomas with distant metastasis. Fifty-eight papillary thyroid tumor specimens (27 papillary thyroid carcinomas with distant metastasis and 31 without metastasis) were examined, and protein expression of pituitary tumor-transforming gene (PTTG), E-cadherin, p27kip1, vascular endothelial growth factor (VEGF)-C, metalloproteinase (MMP) 2, MMP9, chemokine receptor CXCR4, and basic fibroblast growth factor (bFGF) in these tumors was assessed by immunohistochemistry. The clinicopathological variables with diagnostic significance were determined by multivariate analysis, and their diagnostic values were evaluated by ROC curve analysis. PTTG, VEGF-C, MMP2, MMP9, CXCR4, and bFGF were overexpressed in metastatic papillary thyroid carcinomas, whereas p27kip1 expression was elevated only in carcinomas lacking metastasis. Multiple-factor binary ordinal logistic regression analysis revealed that PTTG, VEGF-C, MMP2, and bFGF were independently related to biological metastatic behavior in thyroid tumors, suggesting their potential use as biomarkers. ROC curve analysis showed that among these four proteins, VEGF-C and bFGF were the best diagnostic biomarkers. A VEGF-C and bFGF cluster was the most useful factor for the differential diagnosis between metastatic and non-metastatic papillary thyroid cancers. Thus, the combined use of VEGF-C and bFGF as biomarkers may improve the diagnostic accuracy of papillary thyroid carcinoma and may be useful in multimodal screening programs for the clinical diagnosis of papillary thyroid carcinoma and early detection of papillary thyroid carcinoma with distant metastasis.

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