scholarly journals Prospecting the plant growth–promoting activities of endophytic bacteria Cronobacter sp. YSD YN2 isolated from Cyperus esculentus L. var. sativus leaves

2022 ◽  
Vol 72 (1) ◽  
Author(s):  
Saisai Wang ◽  
Jinbin Wang ◽  
Yifan Zhou ◽  
Yanna Huang ◽  
Xueming Tang
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Mutant Strain ◽  
Organic Phosphate ◽  
Cyperus Esculentus ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Physiological Tests ◽  
Solubilization Ability

Abstract Purpose Plant growth–promoting (PGP) bacteria are an environment-friendly alternative to chemical fertilizers for promoting plant growth and development. We isolated and characterized a PGP endophyte, YSD YN2, from the leaves of Cyperus esculentus L. var. sativus. Methods Specific PGP characteristics of this strain, such as phosphate solubilization ability, potassium-dissolving ability, siderophore and indole-3-acetic acid (IAA) production, and salt tolerance, were determined in vitro. In addition, positive mutants were screened using the atmospheric and room temperature plasma (ARTP) technology, with IAA level and organic phosphate solubility as indices. Furthermore, the effect of the positive mutant on seed germination, biomass production, and antioxidant abilities of greengrocery seedling was evaluated, and the genome was mined to explore the underlying mechanisms. Results The strain YSD YN2 showed a good performance of PGP characteristics, such as the production of indole acetic acid and siderophores, solubilization ability of phosphate, and potassium-dissolving ability. It was recognized through 16S rRNA sequencing together with morphological and physiological tests and confirmed as Cronobacter sp. The strain exposed to a mutation time of 125 s by ARTP had the highest IAA and organic phosphate (lecithin) concentrations of 9.25 mg/L and 16.50 mg/L, 50.41% and 30.54% higher than those of the initial strain. Inoculation of mutant strain YSD YN2 significantly increased the seed germination, plant growth attributes, and the activities of peroxidase (POD) and superoxide dismutase (SOD), respectively, but decreased the content of malondialdehyde (MDA) significantly compared with the control. Furthermore, genome annotation and functional analysis were performed through whole-genome sequencing, and PGP-related genes were identified. Conclusion Our results indicated that the mutant strain YSD YN2 with PGP characteristics is a potential candidate for the development of biofertilizers.

scholarly journals Acetobacter sacchari sp. nov., for a plant growth-promoting acetic acid bacterium isolated in Vietnam

2019 ◽  
Vol 69 (11) ◽  
pp. 1155-1163 ◽  
Author(s):  
Huong Thi Lan Vu ◽  
Pattaraporn Yukphan ◽  
Van Thi Thu Bui ◽  
Piyanat Charoenyingcharoen ◽  
Sukunphat Malimas ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Acetic Acid Bacterium ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Biochemical and Genetic Bases of Indole-3-Acetic Acid (Auxin Phytohormone) Degradation by the Plant-Growth-Promoting Rhizobacterium Paraburkholderia phytofirmans PsJN

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Raúl Donoso ◽  
Pablo Leiva-Novoa ◽  
Ana Zúñiga ◽  
Tania Timmermann ◽  
Gonzalo Recabarren-Gajardo ◽  
...  
Keyword(s):  
Energy Source ◽  
Gene Regulation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Plant Hormone ◽  
Content Type ◽  
Gene Functions ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT Several bacteria use the plant hormone indole-3-acetic acid (IAA) as a sole carbon and energy source. A cluster of genes (named iac) encoding IAA degradation has been reported in Pseudomonas putida 1290, but the functions of these genes are not completely understood. The plant-growth-promoting rhizobacterium Paraburkholderia phytofirmans PsJN harbors iac gene homologues in its genome, but with a different gene organization and context than those of P. putida 1290. The iac gene functions enable P. phytofirmans to use IAA as a sole carbon and energy source. Employing a heterologous expression system approach, P. phytofirmans iac genes with previously undescribed functions were associated with specific biochemical steps. In addition, two uncharacterized genes, previously unreported in P. putida and found to be related to major facilitator and tautomerase superfamilies, are involved in removal of an IAA metabolite called dioxindole-3-acetate. Similar to the case in strain 1290, IAA degradation proceeds through catechol as intermediate, which is subsequently degraded by ortho-ring cleavage. A putative two-component regulatory system and a LysR-type regulator, which apparently respond to IAA and dioxindole-3-acetate, respectively, are involved in iac gene regulation in P. phytofirmans. These results provide new insights about unknown gene functions and complex regulatory mechanisms in IAA bacterial catabolism. IMPORTANCE This study describes indole-3-acetic acid (auxin phytohormone) degradation in the well-known betaproteobacterium P. phytofirmans PsJN and comprises a complete description of genes, some of them with previously unreported functions, and the general basis of their gene regulation. This work contributes to the understanding of how beneficial bacteria interact with plants, helping them to grow and/or to resist environmental stresses, through a complex set of molecular signals, in this case through degradation of a highly relevant plant hormone.

scholarly journals The Effectiveness of Giving Plant PGPR Rhizosphere Bamboo on Cocoa Seeds Germination at The Nursery Level

2021 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Muhammad Yusril Hardiansyah ◽  
Yunus Musa ◽  
Abdul Mollah Jaya
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Block Design ◽  
Plant Growth Promoting Rhizobacteria ◽  
Special Treatment ◽  
Initial Growth ◽  
Seed Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.

scholarly journals Evaluation of the plant-growth-promoting abilities of endophytic bacteria from the psammophyteAmmodendron bifolium

2018 ◽  
Vol 64 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Yanlei Zhu ◽  
Xiaoping She
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Stages ◽  
Bacterial Isolates ◽  
Pellicle Formation ◽  
Radicle Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The objective of this study was to assess the plant-growth-promoting abilities of 45 endophytic bacterial isolates from Ammodendron bifolium through physiological characteristics detection and endophytic bacteria–plant interaction. Each of these isolates exhibited 1 or more plant-growth-promoting traits, but only 11 isolates belonging to the genera Bacillus, Staphylococcus, and Kocuria were capable of promoting seed germination and radicle growth. These results together with the results of the correlation analysis revealed that the completion of seed germination may not be due to IAA production, phosphate solubilization, pellicle formation, and ACC deaminase, protease and lipase production by endophytic bacteria, but may be closely related to amylase and cellulase production. Further, endophytic bacterial isolates with plant-growth-promoting traits may also provide beneficial effects to host plants at different growth stages. Thus, these results are of value for understanding the ecological roles of endophytic bacteria in host plant habitats and can serve as a foundation for further studies of their potential in plant regeneration.

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

scholarly journals Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN

2013 ◽  
Vol 26 (5) ◽  
pp. 546-553 ◽  
Author(s):  
Ana Zúñiga ◽  
María Josefina Poupin ◽  
Raúl Donoso ◽  
Thomas Ledger ◽  
Nicolás Guiliani ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Quorum Sensing ◽  
Plant Growth ◽  
Growth Promotion ◽  
Auxin Signaling ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
Burkholderia Phytofirmans Psjn

Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant–bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) in seed germination and root-shoot development of chickpea (Cicer arietinum L.) under different salinity condition

2016 ◽  
Vol 3 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Mohammad Mosharraf Hossain ◽  
Keshob Chandra Das ◽  
Sabina Yesmin ◽  
Syfullah Shahriar
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Matter Production ◽  
Wide Range ◽  
Positive Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms. Ten isolates of bacteria designated as SS01, SS02, SS03, SS04, SS05, SS06, SS07, SS08, SS09 and SS10 were successfully isolated and morphologically and biochemically characterized. Subsequently to investigate the effect of PGPR isolates on the growth of chickpea, a pot culture experiment was conducted in 2013 at National Institute Biotechnology, Bangladesh net house. Prior to seeds grown in plastic pots, seeds were treated with PGPR isolates and seedlings were harvested after 21 days of inoculation. All the isolates were gram negative in reaction, catalase positive, produced indole acetic acid (IAA) as well as performed phosphate solubilization, able to degrade cellulose and have the adaptability in wide range of temperature and showed positive growth pattern in medium. Most of isolates resulted in a significant increasing of shoot length, root length and dry matter production of shoot and root of chickpea seedlings. Application of PGPR isolates significantly improves the percentage of seed germination under saline conditions. The present study, therefore suggested that the use of PGPR isolates SS04, SS10 and SS08 as inoculants biofertilizers might be beneficial for chickpea cultivation in saline conditionRes. Agric., Livest. Fish.3(1): 105-113, April 2016

scholarly journals Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

2022 ◽  
Vol 1 ◽  
Author(s):  
Isaneli Batista dos Santos ◽  
Arthur Prudêncio de Araújo Pereira ◽  
Adijailton José de Souza ◽  
Elke Jurandy Bran Nogueira Cardoso ◽  
Flaviana Gonçalves da Silva ◽  
...  
Keyword(s):  
Seed Germination ◽  
Quorum Sensing ◽  
Genetic Variability ◽  
Plant Growth ◽  
Bacterial Strains ◽  
Initial Development ◽  
Corn Seed ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Burkholderia sp. is a bacterial genus extremely versatile in the environment and has been reported for a great potential to promote plant growth via different mechanisms. Here we evaluate the plant growth-promoting mechanisms in twenty-six Burkholderia strains. Strains were evaluated for their ability to promote plant growth by means of: indole-3-acetic acid (IAA) production under different conditions of pH, salt stress and the presence or absence of L-tryptophan; exopolysaccharides (EPS) production and quorum sensing (ALH). The strains were also characterized in terms of their genetic variability and species identification through Sanger sequencing. Then, the bacteria most responsive in the greatest number of plant-growth promotion mechanisms were selected for a corn seed germination test. All bacteria synthesized IAA in medium with 0.0 or 5.0 mM of L-tryptophan in combination with either 1 or 5% of NaCl, and pH values of either 4.5 or 7.2. The EPS production was confirmed for 61.54% of the bacterial strains. Quorum sensing also occurred in 92.3% of the selected bacteria. The Jaccard similarity coefficient revealed 16 clusters with high genetic variability between bacterial strains. Bacterial strains were assigned to seven species: B. anthina, B. cepacia, B. gladioli, B. ambifaria, B. graminis, B. heleia, and Burkholderia spp. The corn seed bacterization did not affect the germination velocity index (GSI), as well as the first count of germinated seeds (FC). However, inoculations formulated with B. heleia strain G28, B. gladioli strain UAGC723, and B. graminis strain UAGC348 promoted significant increases in root length, seedling height and fresh and dry seedling phytomass, respectively. These results indicate the high biotechnological potential of several strains in the genus Burkholderia sp. as seed inoculants, favoring germination and seedling initial development.

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