scholarly journals In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Suqi Zou ◽  
Bing Hu
Keyword(s):  
Cell Division ◽  
Optic Nerve ◽  
In Vivo Imaging ◽  
Asymmetric Division ◽  
Adult Zebrafish ◽  
Mature Oligodendrocyte ◽  
Imaging Results ◽  
Perfect Model

AbstractWhether mature oligodendrocytes (mOLs) participate in remyelination has been disputed for several decades. Recently, some studies have shown that mOLs participate in remyelination by producing new sheaths. However, whether mOLs can produce new oligodendrocytes by asymmetric division has not been proven. Zebrafish is a perfect model to research remyelination compared to other species. In this study, optic nerve crushing did not induce local mOLs death. After optic nerve transplantation from olig2:eGFP fish to AB/WT fish, olig2+ cells from the donor settled and rewrapped axons in the recipient. After identifying these rewrapping olig2+ cells as mOLs at 3 months posttransplantation, in vivo imaging showed that olig2+ cells proliferated. Additionally, in vivo imaging of new olig2+ cell division from mOLs was also captured within the retina. Finally, fine visual function was renewed after the remyelination program was completed. In conclusion, our in vivo imaging results showed that new olig2+ cells were born from mOLs by asymmetric division in adult zebrafish, which highlights the role of mOLs in the progression of remyelination in the mammalian CNS.

scholarly journals Role of increased vascular permeability in chemotherapy‐induced alopecia: In vivo imaging of the hair follicular microenvironment in mice

2020 ◽  
Vol 111 (6) ◽  
pp. 2146-2155 ◽  
Author(s):  
Noriko Sagawa ◽  
Yusuke Oshima ◽  
Takahiro Hiratsuka ◽  
Yohei Kono ◽  
Tsuyoshi Etoh ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
In Vivo Imaging

In-vivo imaging results with ultrasound tomography: report on an ongoing study at the Karmanos Cancer Institute

2010 ◽  
Author(s):  
Nebojsa Duric ◽  
Peter Littrup ◽  
Priti Chandiwala-Mody ◽  
Cuiping Li ◽  
Steven Schmidt ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Ongoing Study ◽  
Ultrasound Tomography ◽  
Imaging Results

scholarly journals In Vivo Imaging of the Retina, Choroid, and Optic Nerve Head in Guinea Pigs

2018 ◽  
Vol 43 (8) ◽  
pp. 1006-1018 ◽  
Author(s):  
Ashutosh Jnawali ◽  
Krista M Beach ◽  
Lisa A Ostrin
Keyword(s):  
Optic Nerve ◽  
Optic Nerve Head ◽  
In Vivo Imaging ◽  
Guinea Pigs

scholarly journals In vivo live imaging of postnatal neural stem cells

Development ◽  
2021 ◽  
Vol 148 (18) ◽  
Author(s):  
Alina Marymonchyk ◽  
Sarah Malvaut ◽  
Armen Saghatelyan
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Live Imaging ◽  
Future Directions ◽  
Environmental Signals ◽  
New Information ◽  
Imaging Results

ABSTRACT Neural stem cells (NSCs) are maintained in specific regions of the postnatal brain and contribute to its structural and functional plasticity. However, the long-term renewal potential of NSCs and their mode of division remain elusive. The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific intracellular pathways that allow NSCs to integrate and decode different micro-environmental signals. Lastly, we discuss future directions that may provide answers to unresolved questions regarding NSC physiology.

scholarly journals Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains

2019 ◽  
Vol 218 (6) ◽  
pp. 1810-1823 ◽  
Author(s):  
Hanae Sato ◽  
Bin Wu ◽  
Fabien Delahaye ◽  
Robert H. Singer ◽  
John M. Greally
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Cell Differentiation ◽  
Dna Replication ◽  
Gene Silencing ◽  
In Vivo Imaging ◽  
Histone Variants ◽  
G1 Phase ◽  
Potential Contributor

The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles controlling gene silencing and cell differentiation. Its mechanisms of regional genomic targeting and maintenance through cell division are unknown. Here, we combined in vivo imaging with biochemical and genomic approaches to show that human macroH2A is incorporated into chromatin in the G1 phase of the cell cycle following DNA replication. The newly incorporated macroH2A retargets the same large heterochromatic domains where macroH2A was already enriched in the previous cell cycle. It remains heterotypic, targeting individual nucleosomes that do not already contain a macroH2A molecule. The pattern observed resembles that of a new deposition of centromeric histone variants during the cell cycle, indicating mechanistic similarities for macrodomain-scale regulation of epigenetic properties of the cell.

scholarly journals Longitudinal In Vivo Imaging of Retinal Ganglion Cells and Retinal Thickness Changes Following Optic Nerve Injury in Mice

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e40352 ◽  
Author(s):  
Balwantray C. Chauhan ◽  
Kelly T. Stevens ◽  
Julie M. Levesque ◽  
Andrea C. Nuschke ◽  
Glen P. Sharpe ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Nerve Injury ◽  
Retinal Ganglion Cells ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Retinal Thickness ◽  
Retinal Ganglion ◽  
Optic Nerve Injury

In vivo Imaging and Biodistribution of Multimodal Polymeric Nanoparticles Delivered to the Optic Nerve

Small ◽  
2012 ◽  
Vol 8 (10) ◽  
pp. 1579-1589 ◽  
Author(s):  
James Harrison ◽  
Carole A. Bartlett ◽  
Gary Cowin ◽  
Philip K. Nicholls ◽  
Cameron W. Evans ◽  
...  
Keyword(s):  
Optic Nerve ◽  
In Vivo Imaging ◽  
Polymeric Nanoparticles

Role of manganese in red long-lasting phosphorescence of manganese-doped diopside for in vivo imaging

2013 ◽  
Vol 48 (5) ◽  
pp. 1898-1905 ◽  
Author(s):  
A. Lecointre ◽  
A. Bessière ◽  
K.R. Priolkar ◽  
D. Gourier ◽  
G. Wallez ◽  
...  
Keyword(s):  
In Vivo Imaging

scholarly journals Aggregation via the Red, Dry, and Rough Morphotype Is Not a Virulence Adaptation in Salmonella enterica Serovar Typhimurium

2008 ◽  
Vol 76 (3) ◽  
pp. 1048-1058 ◽  
Author(s):  
A. P. White ◽  
D. L. Gibson ◽  
G. A. Grassl ◽  
W. W. Kay ◽  
B. B. Finlay ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Primary Role ◽  
Wild Type ◽  
Serovar Typhimurium ◽  
Cell Densities ◽  
Two Populations

ABSTRACT The Salmonella rdar (red, dry, and rough) morphotype is an aggregative and resistant physiology that has been linked to survival in nutrient-limited environments. Growth of Salmonella enterica serovar Typhimurium was analyzed in a variety of nutrient-limiting conditions to determine whether aggregation would occur at low cell densities and whether the rdar morphotype was involved in this process. The resulting cultures consisted of two populations of cells, aggregated and nonaggregated, with the aggregated cells preferentially displaying rdar morphotype gene expression. The two groups of cells could be separated based on the principle that aggregated cells were producing greater amounts of thin aggregative fimbriae (Tafi or curli). In addition, the aggregated cells retained some physiological characteristics of the rdar morphotype, such as increased resistance to sodium hypochlorite. Competitive infection experiments in mice showed that nonaggregative ΔagfA cells outcompeted rdar-positive wild-type cells in all tissues analyzed, indicating that aggregation via the rdar morphotype was not a virulence adaptation in Salmonella enterica serovar Typhimurium. Furthermore, in vivo imaging experiments showed that Tafi genes were not expressed during infection but were expressed once Salmonella was passed out of the mice into the feces. We hypothesize that the primary role of the rdar morphotype is to enhance Salmonella survival outside the host, thereby aiding in transmission.

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