Association between changes in molecular biomarkers of cartilage matrix turnover and changes in knee articular cartilage: a longitudinal pilot study

The action of excess retinol on articular cartilage from growing pigs was studied in organ culture. Retinol had little or no effect on explants of articular cartilage alone, but if the explants were cut so as to include some of the marrow tissue in the invasion cavities, or were cultivated near or in contact with capsular tissue, retinol caused extensive degradation of the cartilage matrix, as indicated by loss of metachromatic staining properties. Many chondrocytes were released from their capsules and assumed a fibroblast-like form. Two types of regeneration were seen. In control explants that included part of the invasion zone, cells below the explant laid down a metachromatic matrix; in similar explants cultured in the presence of retinol, a non-metachromatic osteoid-like tissue was formed at this site. There was little recovery when retinol-treated explants were transferred to normal medium, although both osteoid and chondroid tissue were sometimes regenerated.

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Microwave Induced Thermoacoustic Imaging of Osteoarthritis: A Feasibility Study

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.905.498 ◽

2014 ◽

Vol 905 ◽

pp. 498-501

Author(s):

Bo Li Su ◽

Wei Zhi Qi ◽

Xue Liang Xu ◽

L. Huang ◽

X.C. Zhong ◽

...

Keyword(s):

Articular Cartilage ◽

Pilot Study ◽

Absorption Coefficient ◽

Feasibility Study ◽

Two Dimensional ◽

Finger Joints ◽

Thermoacoustic Imaging ◽

The Difference ◽

First Time

We present a pilot study for the first time that microwave-induced thermoacoustic tomography (TAT) has the potential to detect osteoarthritis (OA) in the finger jointsin vitro. In this study, we use rabbit hind feet to imitate humans finger joints, one rabbits hind feet was examined carefully by a TAT scanner, and the two-dimensional (2D) thermoacoustic images were reconstructed by the delay-and-sum algorithm. The difference of absorption coefficient of bone and articular cartilage has been displayed clearly in the reconstruction images.

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Double echo steady state magnetic resonance imaging of knee articular cartilage at 3 Tesla: a pilot study for the Osteoarthritis Initiative

Annals of the Rheumatic Diseases ◽

10.1136/ard.2005.039370 ◽

2006 ◽

Vol 65 (4) ◽

pp. 433-441 ◽

Cited By ~ 199

Author(s):

F Eckstein

Keyword(s):

Magnetic Resonance Imaging ◽

Steady State ◽

Articular Cartilage ◽

Pilot Study ◽

Magnetic Resonance ◽

3 Tesla ◽

Resonance Imaging ◽

Osteoarthritis Initiative

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Structural Variations in Articular Cartilage Matrix Are Associated with Early-Onset Osteoarthritis in the Spondyloepiphyseal Dysplasia Congenita (Sedc) Mouse

International Journal of Molecular Sciences ◽

10.3390/ijms140816515 ◽

2013 ◽

Vol 14 (8) ◽

pp. 16515-16531 ◽

Cited By ~ 9

Author(s):

David Macdonald ◽

Ryan Squires ◽

Shaela Avery ◽

Jason Adams ◽

Melissa Baker ◽

...

Keyword(s):

Articular Cartilage ◽

Early Onset ◽

Cartilage Matrix ◽

Structural Variations ◽

Spondyloepiphyseal Dysplasia ◽

Spondyloepiphyseal Dysplasia Congenita

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INCREASED FIXATION OF SULFUR35 BY CARTILAGE IN VITRO FOLLOWING DEPLETION OF THE MATRIX BY INTRAVENOUS PAPAIN

Journal of Experimental Medicine ◽

10.1084/jem.112.5.743 ◽

1960 ◽

Vol 112 (5) ◽

pp. 743-750 ◽

Cited By ~ 19

Author(s):

T. F. McElligott ◽

J. L. Potter

Keyword(s):

Articular Cartilage ◽

Chondroitin Sulfate ◽

Intravenous Injection ◽

Experimental Situation ◽

Costal Cartilage ◽

Primary Lesion ◽

Cartilage Matrix ◽

Osteoarthritic Cartilage ◽

The Matrix

The uptake in vitro of sulfur-35 by costal cartilage obtained from nine rabbits 11 days after an intravenous injection of crude papain solution was compared with that in costal cartilage from eight normal untreated rabbits. An increased fixation of the isotope was found in treated animals compared with controls. The depletion of cartilage matrix by papain provided an experimental situation to test the hypothesis that the depletion of matrix which occurs in osteoarthritic cartilage can stimulate increased synthesis of chondroitin sulfate. The results give further support to the view that the primary lesion in osteoarthritis occurs in the matrix rather than in the chondrocyte of articular cartilage.

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Tankyrase inhibition preserves osteoarthritic cartilage by coordinating cartilage matrix anabolism via effects on SOX9 PARylation

Nature Communications ◽

10.1038/s41467-019-12910-2 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 6

Author(s):

Sukyeong Kim ◽

Sangbin Han ◽

Yeongjae Kim ◽

Hyeon-Seop Kim ◽

Young-Ran Gu ◽

...

Keyword(s):

Factor Analysis ◽

Stem Cell ◽

Articular Cartilage ◽

Stem Cell Transplantation ◽

Degradation Pathway ◽

Cartilage Matrix ◽

Osteoarthritic Cartilage ◽

Repair Capacity ◽

Dependent Protein ◽

Surgically Induced

Abstract Osteoarthritis (OA) is a prevalent degenerative disease, which involves progressive and irreversible destruction of cartilage matrix. Despite efforts to reconstruct cartilage matrix in osteoarthritic joints, it has been a difficult task as adult cartilage exhibits marginal repair capacity. Here we report the identification of tankyrase as a regulator of the cartilage anabolism axis based on systems-level factor analysis of mouse reference populations. Tankyrase inhibition drives the expression of a cartilage-signature matrisome and elicits a transcriptomic pattern that is inversely correlated with OA progression. Furthermore, tankyrase inhibitors ameliorate surgically induced OA in mice, and stem cell transplantation coupled with tankyrase knockdown results in superior regeneration of cartilage lesions. Mechanistically, the pro-regenerative features of tankyrase inhibition are mainly triggered by uncoupling SOX9 from a poly(ADP-ribosyl)ation (PARylation)-dependent protein degradation pathway. Our findings provide insights into the development of future OA therapies aimed at reconstruction of articular cartilage.

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