scholarly journals Inoculation and colonization of isolated entomopathogenic fungi Beauveria bassiana in rice plants, Oryza sativa L. through seed immersion method

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Etr H. K. Khashaba
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oryza Sativa L ◽  
Pcr Amplification ◽  
Its Region ◽  
Good Method ◽  
Conidial Suspension ◽  
Immersion Method ◽  
Rice Plants ◽  
Egyptian Soil

Abstract Background The aims of the present study were to isolate and identify native entomopathogenic fungi, Beauveria bassiana from the Egyptian soil and to evaluate the artificial establishment of B. bassiana as endophytes in rice plants through seeds immersion. Results Ten soil samples were collected from different cultivated fields at the Ismailia Governorate. Only one sample was positive with a ratio of 10%. Sequencing of the internal transcribed spacer (ITS) region indicated that the isolate obtained from the soil sample belongs to B. bassiana and was registered under the accession no. MN337282. To test the endophytic colonization of B. bassiana, rice seeds were soaked by B. bassiana with a concentration of 5 × 107 spores/ml, to test when B. bassiana become an endophyte in rice plants. The plants were examined for endophytic presence of B. bassiana, 30 days post treatment. PCR amplification using fungal specific primers for a conserved region of β-tubulin gene yielded identical 360 bp products from both B. bassiana and rice treated plants. Conclusion The results showed that seeds immersion with a conidial suspension proved to be a good method to introduce B. bassiana into rice leaves to protect the rice plants against stem borers.

scholarly journals First Report of Mature Citrus Trees Being Affected by Fusarium Wilt in Tunisia

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 566-566 ◽  
Author(s):  
I. Hannachi ◽  
S. Rezgui ◽  
M. Cherif
Keyword(s):  
Fusarium Wilt ◽  
Vascular Tissue ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Its Region ◽  
Conidial Suspension ◽  
Sour Orange ◽  
Basal Cells ◽  
First Report ◽  
Citrus Trees

Citrus is an important crop in Tunisia and over 98% of trees of all varieties are grafted on sour orange rootstock. Since September 2010, unusual wilt symptoms have been observed in Takilsa, Bni-Khaled, and Manzel Bouzalfa fields that eventually caused tree death. The disease was observed on 10- to 25-year-old trees of sweet orange (Citrus sinensis) ‘Washington Navel’ and on ‘Clementine’ tangerines (C. tangerina) ‘Cassar,’ ‘Hernandina,’ and ‘MA3,’ all grafted on sour orange (C. aurantum) ‘Bigarade Gou Tou.’ The most conspicuous symptoms were wilting of sections of the canopy, chlorosis and epinasty of young leaves, and discoloration of vascular tissue. No root rot was observed. The problem was widespread with a disease incidence of 45 to 67%. Similar symptoms were described by Timmer et al. (2) on Mexican lime (C. aurantiifolia) nursery plants and some other species of citrus. Three representative isolates of Fusarium oxysporum Schlechtend.:Fr. from crown were single-spored and identified by the production of characteristic, three- to five-celled, sickle-shaped macroconidia with foot-shaped basal cells, ellipsoid microconidia borne in false heads on short monophialides, and chlamydospores in culture (1). The internal transcribed spacer (ITS) region of rDNA and the elongation factor (TEF 1-α) were amplified with primers ITS1/ITS4 and (TEF1/TEF2), respectively. GenBank accessions of ITS region are KC282838, KC282839, and KC282840, for TEF 1-α region are KF531633, KF537336, and KF537337, showed 99% homology with isolates of F. oxysporum in Fusarium-ID data. Pathogenicity tests were conducted on 7-month-old seedlings of sour orange using 10 plants for each of the three isolates. Prior to inoculation, roots were scraping with a sterile scalpel and plants were dipped in a conidial suspension of F. oxysporum (106 conidia ml–1) for 10 min. Each seedling was planted in a separate pot containing 0.7 liter of sterile soil. Non-inoculated plants with scraped roots dipped in sterile distilled water served as controls. Plants were irrigated and placed in a greenhouse at 24 ± 2°C and 12-h photoperiod. One month after inoculation, leaf chlorosis was observed and 2 months later, 90% of inoculated plants presented a severe wilt. Symptoms on infected plants were similar to those observed in the field. F. oxysporum was successfully re-isolated from the stems, thereby completing Koch's postulates. Genomic DNA was isolated from the re-isolations and PCR amplification of the ITS region was performed with the same primers. There was 100% nucleotide identity with sequences of the original isolates. To our knowledge, this is the first report of fusarium wilt of citrus trees in Tunisia. The pathogen may represent a new form species because previously, the disease was only reported from lime and lemon. References: (1) J. F. Leslie and B. A. Summerell. Page 256 in: The Fusarium Laboratory Manual. Blackwell Publishing Professional, Hoboken, NJ, 2006. (2) L. W. Timmer et al. Phytopathology 72:698, 1982.

scholarly journals The Effect of Grain Type on Virulence of Entomopathogenic Fungi Against Stored Product Pests

2020 ◽  
Vol 10 (8) ◽  
pp. 2970 ◽  
Author(s):  
Spiridon Mantzoukas ◽  
Ioannis Lagogiannis ◽  
Katerina Karmakolia ◽  
Anastasia Rodi ◽  
Maria Gazepi ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Oryza Sativa L ◽  
Abiotic Factors ◽  
Triticum Aestivum L ◽  
Fungal Species ◽  
Abiotic Factor ◽  
Conidial Suspension ◽  
Fungal Virulence ◽  
Stored Product Pests ◽  
Lepidoptera Pyralidae

Fungal virulence is multifaceted and dependent on multiple abiotic factors. The present study represents an investigation of the effect of one such abiotic factor, that of the grain type, on the insecticidal action of three entomopathogenic fungal species, Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae), Metarhizium anisopliae (Metschinkoff) Sorokin (Hypocreales: Clavicipitaceae) and Isaria fumosorosea Wize (Hypocreales: Clavicipitaceae) on larvae of the three very common and destructive stored product pests: the khapra beetle (Trogoderma granarium Everts) (Coleoptera: Dermestidae), the confused flour beetle (Tribolium confusum Jacquelin du Val) (Coleoptera: Tenebrionidae) and the Mediterranean flour moth (Ephestia kuehniella Zeller) (Lepidoptera: Pyralidae). To this end, we selected four different grains, i.e., Triticum aestivum L. (Poales: Poaceae), Oryza sativa L. (Poales: Poaceae), Arachis hypogaea L. (Fabales: Fabaceae) and Vicia faba L. (Fabales: Fabaceae). Bioassays were carried out in the lab, where experimental grains were sprayed with 1 mL of conidial suspension (108 conidia/mL) from each isolate. Mean mortality, median survival time and weight loss of seeds were estimated for each species. Our results suggest that the differences in the efficacy of entomopathogenic fungi were dependent both on the isolates and the grain. The grain type as a factor is equally important to other abiotic factors.

scholarly journals The Effect Of Seed Treatment Duration With Entomopathogenic Fungi Beauveria Bassiana On Seed Germination And Seedling Growth Of Chili

2020 ◽  
Vol 3 (1) ◽  
pp. 25-29
Author(s):  
Trizelia Trizelia
Keyword(s):  
Seed Germination ◽  
Beauveria Bassiana ◽  
Seedling Growth ◽  
Entomopathogenic Fungi ◽  
Treatment Duration ◽  
Seed Treatment ◽  
Conidial Suspension ◽  
Growth Promoters ◽  
Vigor Index ◽  
Germination And Growth

Beauveria bassiana (Balsamo) Vuill. is an entomopathogenic fungi can live  endophytic on different kinds  of plants. B. bassiana also play a role as growth promoters that  improves seed germination and growth of plant.  The effect of increased duration of seed treatment with Beauveria bassiana on seed germination and seedling parameters in chili were assessed in laboratory and field conditions. Chili seeds were soaked in the conidial suspension of B. bassiana in a concentration of 1 x 108 conidial / ml or in sterile distilled water for 3, 6, 9, and 12 h. Seed germination percentages and the vigor index were significantly affected by the application of B. bassiana. Overall, seed germination, the vigor index and plant growth of chili were significantly enhanced as seed treatment duration increased.  B. bassiana can also accelerate seed germination of chili and increase of seedling growth. Soaking the seeds with a B. bassiana suspension for 9 hours is the best time to increase of seed germination and seedling growth of chili

scholarly journals First Report of Wilt of Eucalyptus Caused by Ceratocystis fimbriata in China

Plant Disease ◽  
2014 ◽  
Vol 98 (12) ◽  
pp. 1744-1744 ◽  
Author(s):  
J. Li ◽  
Y. Zhang ◽  
K. C. Xu ◽  
J. Y. Yang ◽  
Y. H. Han ◽  
...  
Keyword(s):  
Sequence Data ◽  
Pcr Amplification ◽  
Its Region ◽  
Molecular Characteristics ◽  
Malt Extract ◽  
Conidial Suspension ◽  
Ceratocystis Fimbriata ◽  
First Report ◽  
Symptomatic Tissue ◽  
Black Discoloration

Eucalyptus spp., most of which are native to Australia, are one of the world's most commonly planted forestry crops, and have been widely planted in the tropics and subtropics. Eucalyptus trees are distributed in more than 600 counties in 19 provinces/regions throughout China, especially in the southern regions of the country (1). From April of 2013 to May of 2014, eucalyptus trees were noted to be wilting and dying rapidly in Kunming, Yunnan Province (25°02′ N, 102°42′ E), southwest China. Symptomatic trees typically have many wilted or dead leaves hanging throughout the tree or on some major branches, sometimes followed by death of the whole tree. Dark brown to black discoloration can be seen in the woody xylem, and elongated cankers were also present on some affected trees. A fungus was consistently isolated from the leaves and cankers when symptomatic tissue was incubated between two slices of fresh carrot root. Spore masses were removed from the apices of perithecia, transferred to malt extract agar medium (MEA), and incubated at 25°C. Perithecia developed on the medium, and were black, globose, 212.5 to 242.4 × 207.5 to 254.2 μm, and possessed a long black neck (952.3 to 1,303.3 μm). Ascospores had the typical “hat” morphology and were 4.3 to 5.2 × 3.0 to 3.8 μm. Cylindrical endoconidia (11.2 to 22.2 × 3.9 to 6.1 μm) were found. Chlamydospores produced on media were ovoid and smooth, and were 7.8 to 9.7 × 9.9 to 12.8 μm. Chains of barrel-shaped conidia were not found. PCR amplification and sequencing of the ITS region of rDNA were carried out for one isolate, E2-2, using the procedures of Thorpe et al. (3). Analysis of ITS sequence data (GenBank Accession No. KJ511481) showed that the isolates were 99% homologous to the isolate of C. fimbriata from diseased Colocasia esculenta in Cuba, China, and Hawaii (AY526304 to 06) by BLAST analysis. Thus, the fungus was identified as C. fimbriata based on morphological and molecular characteristics. Pathogenicity tests were made on 1-year-old E. grandis seedlings as follows. A conidial suspension of each isolate was diluted to 106 conidia/ml, and 0.2 ml was injected into wounds on three petioles on each of five healthy plants of E. grandis, and control seedlings were injected with sterile water that had been placed on MEA plates. The seedlings were incubated at 25°C in moist chambers. After 3 days, all inoculated E. grandis plants showed dark brown to black discoloration in the leaf axils. After 5 days, leaf wilting was present. C. fimbriata was re-isolated from all inoculated symptomatic tissue. No symptoms were visible in the control plants and no fungus was isolated from them. Ceratocystis wilt was first observed in eucalyptus in 1997 in the state of Bahia. This was followed by a report of C. fimbriata causing wilt of E. grandis in the Republic of Congo, Uganda, and Uruguay (2). Chen et al. reported two species of Ceratocystis, C. acaciivora and a previously undescribed species C. chinaeucensis, from eucalyptus plantations in Guangdong Province in China (1). To our knowledge, this is the first report of C. fimbriata causing wilt of eucalyptus in China. References: (1) S. F. Chen et al. Fungal Diversity 58:267, 2011. (2) F. A. Ferreira et al. Fitopatol. Bras. 24:284, 1999. (3) D. J. Thorpe et al. Phytopathology 95:316, 2005.

scholarly journals SELEÇÃO DE ISOLADOS DE FUNGOS ENTOMOPATOGÊNICOS PARA O CONTROLE DE Hedypathes betulinus E AVALIAÇÃO DA PERSISTÊNCIA

FLORESTA ◽  
2011 ◽  
Vol 41 (3) ◽  
Author(s):  
Maria Sílvia Pereira Leite ◽  
Edson Tadeu Iede ◽  
Susete Do Rocio Chiarello Penteado ◽  
Scheila Ribeiro Messa Zaleski ◽  
Joelma Melissa Malherbe Camargo ◽  
...  
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Control Plot ◽  
Ilex Paraguariensis ◽  
Conidial Suspension

Hedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) é a principal praga da cultura da erva-mate. Para o seu controle, avaliou-se em laboratório e campo a utilização de fungos entomopatogênicos. Em laboratório, foi avaliada a infectividade de isolados dos fungos Beauveria bassiana (Bals.) Vuillemin, 1912; B. brongniartii (Sacc.) Petch, 1926; Metarhizium anisopliae (Metsch.) Sorokin, 1883, e Paecilomyces (=Isaria) sp. Bainier, 1907, em adultos de H. betulinus. Os insetos foram imersos nas suspensões fúngicas na concentração de 107 conídios/mL. Após a aplicação, eles foram mantidos em laboratório para registro da mortalidade. Verificou-se que B. bassiana (CG 716) foi o mais infectivo, com 100% de mortalidade. Foi estimada a CL50 do isolado CG 716 de B. bassiana com cinco concentrações, variando de l05 a l09 conídios/mL, mais a testemunha. A CL50 estimada foi de 2,0x106 conídios/mL, variando de 6x105 a 5x106 conídios/mL. Foi avaliada a persistência do isolado CG 716 de B. bassiana formulado à base de óleo emulsionável em campo, verificando-se alta mortalidade nas primeiras três semanas após a aplicação do fungo, variando de 95 a 78%, decaindo para 65 a 47%, um e dois meses após a aplicação, respectivamente.Palavras-chave: Controle biológico; broca-da-erva-mate; CL50. AbstractEntomopathogenic fungi in the control of Hedypathes betulinus and evaluation of persistence. Hedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) is the main pest of “erva-mate” (Ilex paraguariensis) culture. Focusing its control, it was evaluated in laboratory and field the entomopathogenic fungi using. In laboratory, it was evaluated the infectiveness of the strains of the following fungi: Beauveria bassiana (Bals.)  Vuillemin, B. brongniartii (Sacc.) Petch, Metarhizium anisopliae (Metsch.), Sorokin and Paecilomyces (= Isaria) sp. Bainier, in adults specimen of H. betulinus. The insects were immersed in a conidial suspension at a concentration of 107 conidia mL-1. After the application the insects had been kept at laboratory for mortality register. It was verified that B. bassiana (CG 716) was more infective, reaching to 100% of mortality. It estimated LC50 of isolated CG 716 of B. bassiana with five concentrations and a variation of l05 to l09 conidia mL-1, along the control plot. The estimated LC50 was 2,0 x 106 conidia mL-1, ranging from 6 x 105 to 5 x 106 conidia mL-1. The persistence of strain CG 716 of B. bassiana - formulate oil was evaluated in field, producing the highest mortality, at the first three weeks after the application of the fungus, with a variance of 95 to 78 %, with a decreasing of 65 to 47% , along one and two months after the application , respectively.Keywords: Biological control; erva-mate pest; LC50,.

scholarly journals Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Besma Hamrouni Assadi ◽  
Sabrine Chouikhi ◽  
Refki Ettaib ◽  
Naima Boughalleb M’hamdi ◽  
Mohamed Sadok Belkadhi
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Tobacco Plant ◽  
Economic Losses ◽  
Native Strain ◽  
Lecanicillium Muscarium ◽  
Southern Tunisia ◽  
Significant Efficacy ◽  
Nesidiocoris Tenuis

Abstract Background The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia. Results In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6). Conclusions It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ammarah Hami ◽  
Rovidha S. Rasool ◽  
Nisar A. Khan ◽  
Sheikh Mansoor ◽  
Mudasir A. Mir ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Pcr Amplification ◽  
Its Region ◽  
Fungal Species ◽  
Pathogenicity Test ◽  
Kashmir Valley ◽  
Fusarium Equiseti ◽  
Infected Root ◽  
Fusarium Sp ◽  
High Degree

AbstractChilli (Capsicum annuum L.) is one of the most significant vegetable and spice crop. Wilt caused by Fusarium Sp. has emerged as a serious problem in chilli production. Internal transcribed spacer (ITS) region is widely used as a DNA barcoding marker to characterize the diversity and composition of Fusarium communities. ITS regions are heavily used in both molecular methods and ecological studies of fungi, because of its high degree of interspecific variability, conserved primer sites and multiple copy nature in the genome. In the present study we focused on morphological and molecular characterization of pathogen causing chilli wilt. Chilli plants were collected from four districts of Kashmir valley of Himalayan region. Pathogens were isolated from infected root and stem of the plants. Isolated pathogens were subjected to DNA extraction and PCR amplification. The amplified product was sequenced and three different wilt causing fungal isolates were obtained which are reported in the current investigation. In addition to Fusarium oxysporum and Fusarium solani, a new fungal species was found in association with the chilli wilt in Kashmir valley viz., Fusarium equiseti that has never been reported before from this region. The studies were confirmed by pathogenicity test and re-confirmation by DNA barcoding.

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