Vitamin C supplementation during chronic variable stress exposure modulates contractile functions of testicular artery and sperm parameters in male Wistar rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

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The Effect of Endurance Swimming Plus Vitamin C Supplement on Oxidative Stress and Muscles Damage Indices in Male Wistar Rats

Avicenna Journal of Medical Biochemistry ◽

10.17795/ajmb-34241 ◽

2016 ◽

Vol In Press (In Press) ◽

Author(s):

Leila Vesali-Akbarpour ◽

Mohammad Ali Samavati-Sharif

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Wistar Rats ◽

Damage Indices ◽

Male Wistar Rats

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Protective effect of vitamin C against ivermectin induced nephrotoxicity in different age groups of male wistar rats: bio-histopathological study

Anatomy & Cell Biology ◽

10.5115/acb.21.124 ◽

2021 ◽

Author(s):

Shereen E. Tawfeek ◽

Ayat M. Domouky ◽

Reham H. Abdel-Kareem

Keyword(s):

Vitamin C ◽

Protective Effect ◽

Wistar Rats ◽

Age Groups ◽

Histopathological Study ◽

Male Wistar Rats

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Vitamin C decreases the obesogenic and hyperglycemic effect of invert sugar in prediabetic rats

Revista de Nutrição ◽

10.1590/1678-98652017000100003 ◽

2017 ◽

Vol 30 (1) ◽

pp. 23-32 ◽

Cited By ~ 1

Author(s):

Patrícia MOLZ ◽

Alana Nunes RAEL ◽

Maiara de Queiroz FISCHER ◽

Luana Beatriz LIMBERGER ◽

Daniel PRÁ ◽

...

Keyword(s):

Vitamin C ◽

Glucose Tolerance ◽

Body Mass ◽

Wistar Rats ◽

Area Under The Curve ◽

Tolerance Test ◽

Invert Sugar ◽

Future Studies ◽

Fasting Glycemia ◽

Male Wistar Rats

ABSTRACT Objective: To evaluate whether vitamin C can help to prevent obesity and hyperglycemia in Wistar rats treated with excess invert sugar to induce prediabetes. Methods: One hundred-day-old Male Wistar rats with a mean weight of 336.58±23.43g were randomly assigned to the following groups: (1) control, receiving water (C); (2) invert sugar control, receiving a 32% watery solution of invert sugar; (3) vitamin C control, receiving a watery solution of vitamin C (60mg/L), and (4) vitamin C plus invert sugar, receiving a watery solution of vitamin C and invert sugar. All animals had access to chow and water ad libitum and were treated for 17 weeks. Prediabetes was assessed according to two criteria: obesity (based on body mass indexand peritoneal fat content) and impaired glucose tolerance (assessed by the intraperitoneal glucose tolerance test and expressed as area under the curve) . Results: Group invert sugar control gained significantly more weight (p=0.035) and visceral fat (p<0.001) than groups vitamin C control and vitamin C plus invert sugar. Consequently, groups vitamin C control and vitamin C plus invert sugar had gained as little body mass index as group C by the end of the experiment. Vitamin C decreased the fasting glycemia of both groups supplemented with vitamin C and normalized the glucose tolerance of group vitamin C plus invert sugar, whose area under the curve matched that of group C. Conclusion: Vitamin C has anti-obesogenic and glycemia-lowering effects in Wistar rats, which might be promising to prediabetics. Future studies are needed to understand the anti-obesogenic and anti-hyperglycemic mechanisms of vitamin C in prediabetes.

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Effect of Resistance Exercise and Vitamin C Intake on Expression of Telomerase Reverse Transcriptase and Telomere Repeat Binding Factor-2 Genes and the Diameter and Number of Myofibrils in Old Rats

Complementary Medicine Journal ◽

10.32598/cmja.10.4.1046.1 ◽

2021 ◽

Vol 10 (4) ◽

pp. 396-409

Author(s):

Yousef Shirkhani ◽

◽

Maghsoud Peeri ◽

Mohammad Ali Azarbayjani ◽

Hassan Matinhomaee ◽

...

Keyword(s):

Skeletal Muscle ◽

Vitamin C ◽

Resistance Exercise ◽

Wistar Rats ◽

Telomere Repeat ◽

Vitamin Intake ◽

Binding Factor ◽

Male Wistar Rats ◽

Old Rats ◽

The Difference

Objective: Cell aging is one of the most important and fundamental step in cellular behavior and reduces muscle mass and myofibrils. This study aims to investigate the effect of resistance exercise along with vitamin C consumption on the expression of Telomerase Reverse Transcriptase (TERT) and Telomere Repeat Binding Factor-2 (TRF2) genes and the diameter and number of skeletal muscle myofibrils in old male Wistar rats. Methods: This is an experimental study using control groups. Twenty-five male Wistar rats (280-320 g and aged 24 weeks) were randomly divided into five groups of young control, old+resistance exercise, old+vitamin intake, old+resistance exercise+vitamin intake, and old control. In the supplementation groups, rats received liposomal vitamin C daily by gavage per body weight. Weight attached to the tail gradually increased during 8 weeks of exercise (weeks 1 and 2 by 50%; weeks 3 and 4 by 50%; weeks 5 and 6 by 75%; weeks 7 and 8 by 100% of total body weight). Exercises were performed at 3-5 sets of 8-12 repetitions, 3 or 4 days a week. The Quantitative polymerase chain reaction method was used to evaluate the expression of TERT and TRF2 genes in muscle tissue. One-way ANOVA was used to examine the difference between the groups and Tukey’s post hoc test was used to determine between which groups the difference was significant. Results: Aging significantly reduced the expression of TERT, TRF2 genes and the diameter and number of myofibrils in skeletal muscle of rats (P=0.001). Resistance exercise along with vitamin C intake had no significant effect on TERT and TRF2 expression (P≥0.05), but caused a significant increase in the diameter and number of myofibrils (P= 0.001). Conclusion: Eight weeks of resistance exercise along with vitamin C supplementation can significantly increase the number and diameter of skeletal muscle myofibrils in old rats.

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Effect of vitamin C on liver regeneration after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats

International Surgery Journal ◽

10.18203/2349-2902.isj20210358 ◽

2021 ◽

Vol 8 (2) ◽

pp. 489

Author(s):

Reno Rudiman ◽

Handy Wing ◽

Nurhayat Usman

Keyword(s):

Lipid Peroxidation ◽

Liver Injury ◽

Vitamin C ◽

Liver Regeneration ◽

Partial Hepatectomy ◽

Wistar Rats ◽

Liver Weight ◽

Potential Effect ◽

Regeneration Rate ◽

Male Wistar Rats

Background: The aim of this study is to evaluate the potential effect of vitamin C as an antioxidant on liver regeneration after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats.Methods: A total of 24 male Wistar rats were divided into four groups, each group consisted of 6 rats: group A (control, partial hepatectomy/PHx alone), group B (PHx and vitamin C 250 mg/kg BW), group C (acetaminophen 500 mg/kg BW and PHx), and group D (acetaminophen 500 mg/kg BW with PHx and vitamin C 250 mg/kg BW). Subtoxic dose of acetaminophen was given 24 hours before partial hepatectomy. Vitamin C was given orally via oral gavage for 6 consecutive days after partial hepatectomy. POD 7, all animals were terminated and performed laparotomy to obtain liver tissue for measurement of liver weight and regeneration rate, blood samples for malondialdehyde (MDA) as a lipid peroxidation measurement and histopathological investigation.Results: The means of regeneration rate in vitamin C groups were significantly higher compared to non-vitamin C group (p<0.05). Similar result, the means of MDA values in vitamin C groups were significantly lower compared to non-vitamin C group (p<0.05). This result suggests a protective effect of vitamin C against lipid peroxidation. Histopathological changes in liver cells were statistically difference between vitamin C groups and non-vitamin C groups (p<0.05).Conclusions: Our results indicate that vitamin C administration promotes liver regeneration and inhibits lipid peroxidation after partial hepatectomy and acetaminophen-induced liver injury in Wistar rats.

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Lophira lanceolata protects testicular and spermatological damages induced by cisplatin in male Wistar rats

Clinical Phytoscience ◽

10.1186/s40816-020-00221-9 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Solomon Tsekohol Agu ◽

Christian Okechukwu Ezihe ◽

Paul Friday Itodo ◽

Hyacinth Adakole Abu

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Antineoplastic Agent ◽

The Body ◽

Sperm Parameters ◽

Protective Effects ◽

Testicular Damage ◽

Stress Injury ◽

Male Wistar Rats ◽

Lophira Lanceolata

Abstracts Background Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. Methods The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. Results The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p < 0.05) decreased in the CP treated group relative to the control. However, there was an increase in the weight of the organs in the LLLE pretreated groups. The photomicrographs showed degenerative changes in the testicular tissues of the rats administered CP alone whereas the group pretreated with the LLLE showed amelioration induced by the CP. Our study revealed that CP treatment has deleterious effects on sperm parameters and testicular tissues and the accessory sex organs (Epididymis, prostate, seminal vesicles) of the rats. Oral administration of LLLE at 200 and 400 mg/kg bodyweight for 26 days conferred protective effects against testicular damage induced by CP. Conclusion This study revealed that pretreatment with LLLE protected against CP-induced testicular toxicity.

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