scholarly journals Molecular identification and antimicrobial activity of endophytic fungi isolated from Heritiera fomes (Buch. -Ham), a mangrove plant of the Sundarbans

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Tauhidur Rahman Nurunnabi ◽  
Sabiha Sarwar ◽  
Farah Sabrin ◽  
Farzana Alam ◽  
Lutfun Nahar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Antimicrobial Agents ◽  
Sequence Data ◽  
Micrococcus Luteus ◽  
Mangrove Plant ◽  
Nucleotide Sequence Data ◽  
Methanolic Extracts ◽  
Heritiera Fomes ◽  
The Sundarbans

Abstract Background Endophytic fungi play a vital role in plant defense system by secreting various antimicrobial agents. To evaluate the antimicrobial activity of the endophytic fungi of the mangrove plant Heritiera fomes (Buch. -Ham), plants were collected from the Sundarbans, Bangladesh. The endophytic fungi were subsequently isolated and identified. Results Fifty-five endophytic fungi were isolated from the leaves, root, and bark of H. fomes (Buch. -Ham). Genomic DNA was extracted for PCR (polymerase chain reaction) by specific primers to multiply ITS region and sequences were determined. Nucleotide sequence data were submitted to the Genbank and accession number for each fungal strain was obtained. Antimicrobial activity of the ethyl acetate (EtOAc) and methanolic extracts of eleven species from both fermentation and mycelium, respectively, were analyzed by microtiter plate-based antimicrobial assay incorporating resazurin as an indicator of cell growth against two Gram-positive bacteria namely Staphylococcus aureus NCTC 12981 and Micrococcus luteus NCTC 7508, two Gram-negative bacteria namely Escherichia coli NCTC 12241 and Pseudomonas aeruginosa NCTC 12903, and a fungus Candida albicans ATCC 90028. All the endophytic fungal extracts exhibited antimicrobial activities against more than one-tested pathogenic microbial strains. Overall, methanolic extracts showed greater activity than EtOAc extracts. Pseudopestalotiopsis camelliae-sinensis, Pestalotiopsis microspora, and Penicillium copticola were the most active endophytic fungal strains and exhibited strong inhibitory activity against the microorganisms under investigation and their MIC values ranged from 0.0024 to 5.0 mg/mL. Methanolic extracts of both P. camelliae-sinensis and P. microspora showed the highest antibacterial activity (MIC value of 0.0024 mg/mL) against P. aeruginosa NCTC 12903. Conclusion This study showed that the isolated and identified endophytic fungi from H. fomes (Buch. -Ham) could be potential sources of antimicrobial agents.

scholarly journals Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest

2020 ◽  
Vol 20 (3) ◽  
pp. 419-425 ◽  
Author(s):  
Tauhidur R. Nurunnabi ◽  
Farah Sabrin ◽  
Dilara I. Sharif ◽  
Lutfun Nahar ◽  
Md. H. Sohrab ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Mangrove Forest ◽  
Mangrove Plant ◽  
Sonneratia Apetala ◽  
Sundarbans Mangrove ◽  
The Sundarbans

scholarly journals Identification of Ciprofloxacin-ResistantCampylobacter jejuni by Use of a Fluorogenic PCR Assay

2000 ◽  
Vol 38 (11) ◽  
pp. 3971-3978 ◽  
Author(s):  
David L. Wilson ◽  
Sheila R. Abner ◽  
Thomas C. Newman ◽  
Linda S. Mansfield ◽  
John E. Linz
Keyword(s):  
Antimicrobial Agents ◽  
Sequence Data ◽  
Dna Gyrase ◽  
Species Level ◽  
Primary Target ◽  
Pcr Assay ◽  
Nucleotide Sequence Data ◽  
Rapid Pcr ◽  
Fluoroquinolone Antibiotics ◽  
Ciprofloxacin Resistance

Fluoroquinolones are one class of antimicrobial agents commonly used to treat severe Campylobacter jejuni infection.C. jejuni strains resistant to high levels of the fluoroquinolone ciprofloxacin (MIC ≥16 μg/ml) have been predominantly characterized with a C→T transition in codon 86 ofgyrA. The gyrA gene encodes one subunit of DNA gyrase, which is a primary target for fluoroquinolone antibiotics. This study establishes a rapid PCR-based TaqMan method for identifying ciprofloxacin-resistant C. jejuni strains that carry the C→T transition in codon 86 of gyrA. The assay uses real-time detection, eliminating the need for gel electrophoresis. Optimization of the assay parameters using purified Campylobacter DNA resulted in the ability to detect femtogram levels of DNA. The method should be useful for monitoring the development of ciprofloxacin resistance in C. jejuni. Compiled nucleotide sequence data on the quinolone resistance-determining region of gyrA inCampylobacter indicate that sequence comparison of this region is a useful method for tentative identification ofCampylobacter isolates at the species level.

scholarly journals ISOLASI MIKROBA ENDOFITIK DARI TANAMAN OBAT SAMBUNG NYAWA Gynura procumbens) DAN ANALISIS POTENSINYA SEBAGAI ANTIMIKROBA

2007 ◽  
Vol 13 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Rumella Simarmata ◽  
Sylvia Lekatompessy ◽  
Harmastini Sukiman
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Endophytic Bacteria ◽  
Antimicrobial Agents ◽  
Inhibition Zone ◽  
Pseudomonas Sp ◽  
E Coli ◽  
Beneficial Effects ◽  
Medical Plants ◽  
Promising Source

Sambung nyawa (Gynura procumbens) has many beneficial effects to human health, such as decreasing blood pressure, maintaining blood sugar level (hypoglycaemic), decreasing cholesterol, a remedy for kidney trouble, antibacterial and lessen the inflamation (antiinflamation). This research was undertaken to discover the potency of endophytic microbes from sambung nyawa as antimicrobial agents. The purpose of this research was to screen the endophytic bacteria and the endophytic fungi having antimicrobial activity, which were isolated from stems, leaves, roots and fruits of medical plants, sambung nyawa. The antimicrobial activity was determined by measuring the growth inhibition of pathogenic microbes i.e Candida albicans, Escherichia coli, Pseudomonas sp. and Bacillus subtilis. A total of 38 isolates of bacteria and 15 isolates of fungi were obtained from sambung nyawa. Analysis demonstrated that, 45 percent isolates of bacteria and 20 percent isolates of fungi exhibited inhibitory activity. Antimicrobial activity was found in 21 percent of the isolates that inhibited the growth of C. albicans, E. coli, Pseudomonas sp., and B. subtilis, whereas 24 percent of isolates had activity only against B. subtilis. Isolate of endophytic bacteria USN 1.1 and USN 2.3 showed the most significant of inhibition zone. The Inhibition zone of the isolate USN 1.1 to C. albicans, E. coli, Pseudomonas sp, and B. subtilis were 2.318 cm2, 0.969 cm2, 0.796 cm2, and 0.381 cm2, respectively. The Inhibition zone of the isolate USN 2.3 to C. albicans, E. coli, Pseudomonas sp., and B. subtilis were 3.01 cm2, 0.519 cm2, 0.588 cm2 and 0.83 cm2, respectively. These results indicated that endophytic bacteria and endophytic fungi could be a promising source for antimicrobial agents.

scholarly journals A PRELIMINARY OBSERVATION ON AN EXPLICIT ANTIMICROBIAL ACTION OF MANGROVE PLANTS ON PSEUDOMONAS AERUGINOSA

2019 ◽  
pp. 226-230
Author(s):  
TAMANNA SULTANA ◽  
ARUP KUMAR MITRA ◽  
SATADAL DAS
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Microtiter Plate ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Mangrove Plant ◽  
E Coli ◽  
Mangrove Plants

Objectives: Due to emerging drug-resistant microorganisms throughout the world, newer antimicrobial agents should be looked for. Plants are enriched with different bioactive chemicals. In this study, we searched antibacterial activities of some mangrove plant extracts against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Methods: In the present study, the antimicrobial activity of the leaves of Bruguiera gymnorhiza, Excoecaria agallocha, Avicennia alba, and Aegialitis rotundifolia was evaluated against a few reference pathogenic bacterial strains, namely, P. aeruginosa ATCC 27853, E. coli ATCC 25922, S. aureus ATCC 29213, and multidrug-resistant bacterial strains E. coli extended-spectrum beta-lactamases strain. Aqueous, ethanolic, methanolic, and dimethyl sulfoxide (DMSO) extracts were studied. The antimicrobial activities of the organic solvent extracts on the various test microorganisms were investigated using agar well diffusion technique followed by determination of minimum inhibitory concentration values by serial dilution in a microtiter plate. Results: Ethanol and DMSO extracts of B. gymnorhiza exhibited promising antimicrobial activity followed by extracts of A. alba and E. agallocha. Among all microorganisms studied, P. aeruginosa ATCC 27853 showed significant growth inhibition with ethanol and DMSO extracts. Conclusion: Extracts of some mangrove plants, particularly, B. gymnorhiza showed very good antimicrobial activities against common microbial agents causing human infections and in general mangrove plants appear to act better on P. aeruginosa.

Antioxidant and anti-inflammatory activity of Heritiera fomes (Buch.-Ham), a mangrove plant of the Sundarbans

2019 ◽  
Vol 20 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Md. Emdadul Islam ◽  
Kazi Mohammed Didarul Islam ◽  
Md. Morsaline Billah ◽  
Rana Biswas ◽  
Md. Hossain Sohrab ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Mangrove Plant ◽  
Heritiera Fomes ◽  
Anti Inflammatory ◽  
The Sundarbans

scholarly journals ANTIMICROBIAL SCREENING OF HETEROPHRAGMA ADENOPHYLLUM EXTRACTS AND EFFECTS OF LIGHT IRRADIATION

2012 ◽  
Vol 2 ◽  
pp. 124-133
Author(s):  
Muhammad Shoaib Akhtar ◽  
Keyword(s):  
Health Care ◽  
Antimicrobial Activity ◽  
Primary Health Care ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Antidiabetic Agents ◽  
Methanolic Extracts ◽  
Primary Health ◽  
Crude Drugs ◽  
Tract Infections

Medicinal plants have considerable widespread use as crude drugs in the primary health care of communities. Various plants have already been tested for their antimicrobial activity to discover new antimicrobial agents. In this investigation antimicrobial activities of aqueous and methanolic extracts of Heterophragma adenophyllum leaves and seeds were screened as these have been used since centuries in traditional medicine for the treatment of skin and urinary tract infections and also as antidiarrheol and antidiabetic agents

scholarly journals Synthesis of new Imidazole Derivatives as effective Antimicrobial Agents

2017 ◽  
Vol 5 (01) ◽  
pp. 01-09
Author(s):  
Bipin Kumar Verma ◽  
Sunil Kapoor ◽  
Umesh Kumar ◽  
Savita Pandey ◽  
Priti Arya
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Micrococcus Luteus ◽  
Bacterial Strains ◽  
Standard Drug ◽  
Mass Spectral ◽  
Analytical Technique ◽  
Imidazole Derivatives ◽  
Modern Analytical Technique ◽  
Anti Fungal

In the present work, some new imidazole derivatives (3i-xii) were synthesized as per design synthetic protocol scheme. The structures of newly prepared compounds were confirmed by modern analytical technique (IR, 1H-NMR, Mass spectral data) and elemental analysis, results found in full agreement with their assigned structures. All the synthetic compounds were screened for their antimicrobial activity against bacterial strains viz. Escherichia coli (E. coli, MTCC 2961), Staphylococcus aureus (S. aureus, MTCC 3160), Bacillus subtilis (B. subtilis, MTCC 121), Klebsiella pneumoniae (K. pneumoniae, MTCC 3040) and Micrococcus luteus (M. luteus, MTCC 7527)) and fungal strains viz. Candida albicans (C. albicans, MTCC 227), Aspergillus niger (A. niger, MTCC 277) and Aspergillus flavus (A. flavus, MTCC 418) ; results showed good to remarkable activity. The MIC (minimum inhibitory concentration) values were determined by comparison to ciprofloxacin (anti-bacterial) and fluconazole (anti-fungal) as standard drug. Among them, compound 3iv and 3x exhibited notable antimicrobial activity. These compounds may be used as new template for the searching of potential antimicrobial agents.

Antimicrobial Activity of Actinomycetes Isolated from Paya Maga, Sarawak

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Ng Yik Han Han ◽  
Ting Jen Yi ◽  
Yeo Tiong Chia
Keyword(s):  
Antimicrobial Activity ◽  
Broad Spectrum ◽  
Antimicrobial Agents ◽  
Micrococcus Luteus ◽  
Culture Method ◽  
Inhibition Zone ◽  
Yeast Saccharomyces Cerevisiae ◽  
Gram Positive Bacteria ◽  
Rdna Sequences ◽  
16S Rdna Sequences

A study was carried out to evaluate the antimicrobial activity of 51 actinomycetes strains isolated from environmental samples collected during an expedition at Paya Maga, located in Ulu Trusan, Lawas, Sarawak. These actinomycetes strains were isolated from 20 soil and 15 plant samples. Their ability to inhibit the growth of Gram positive bacteria (Staphylococcus aureus, Micrococcus luteus), Gram negative bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) were tested by using co-culture method. Twenty five percent of the isolates (13 out of 51) were active against at least one bacteria or one yeast standard strain while 18 (35%) were active against at least two standard strains, indicating broad spectrum activities. The results also showed that 23% (7 strains) of the positive isolates with single or broad spectrum activities were able to show better result in comparison to 100 mg/L of Chloramphenicol and Nystatin used (by forming inhibition zone more than 20 mm in diameter). The 31 inhibitory strains were sequenced and partial 16S rDNA sequences were derived for taxonomic identification. The majority of these strains (27 out of 31) belong to the family of Streptomycetaceae, followed by 2 strains from Streptosporangiaceae and 1 strain each from Nocardiaceae and Pseudonocardiaceae. Sixteen of the isolates can only be classified up to the genus level so they are potentially novel species which are targets for further study to isolate antimicrobial agents.

scholarly journals Ecology of Sundarban, Bangladesh

2013 ◽  
Vol 8 (1-2) ◽  
pp. 35-47 ◽  
Author(s):  
MR Rahman ◽  
M Asaduzzaman
Keyword(s):  
Plant Species ◽  
Sea Water ◽  
Demersal Fish ◽  
Adjacent Area ◽  
Mangrove Plant ◽  
Tidal Waves ◽  
Excoecaria Agallocha ◽  
Heritiera Fomes ◽  
Monitor Lizard ◽  
The Sundarbans

Sundarban is the largest mangrove wetland in the world. It covers an area of about 1mha, of which 60% is located in Bangladesh and the remaining western portion, comprising 40%, lies in India. Mangrove ecosystems are of great ecological significance in the tropical and sub-tropical coast. They protect our coast from heavy wind, tidal waves, coastal erosion and sea water intrusion, generate substantial quantities of fishery resources and provide many useful forestry products. The Sundarban ecosystem supports rich fisheries diversity. This ecosystem support 27 families and 53 species of pelagic fish, 49 families 124 species of demersal fish, 5 families and 24 species of shrimps, 3 families and 7 species of crabs, 8 species of lobster. A total 334 plants, 165 algal, 13 special orchids, 17 fern, 87 monocotyledon and 230 dicotyledon belonging to 245 genera and 75 families from the sundarbans and adjacent area are found available. The principal tree species is Sundry (Heritiera fomes) which covers about 73% to total landmass and the second species is Gewa (Excoecaria agallocha) which covers about 16% of total forest area. The plant species include 35 legumes, 29 grasses, 19 sedges, and 18 euphorbias. Of the 50 true mangrove plant species recorded throughout the globe, the Sundarbans alone contain 35 species. The magnificent among the animals on land is Royal Bengal Tiger, Spotted deer, barking deer and wild boars are there in plenty. Besides those jungle cats, fishing cat, civet cat, monkey, bengal fox, jackle, water monitor, monitor lizard and snakes are important faunal spp. Moreover, abundant of the Sundarbans are purple heron, pond heron, cattle egret, little egret, open billed stork, smaller adjutant stork, brahmini kite, spotted dove, rose ringed parakeet, crow pheasant, wood pecker, bee eater, drongo, pide myna, jungle myna, bulbul, tailor bird, magpie robin, sparrow etc., Otherwise, recorded that wild Buffalo, 2 species of deer, javan rhinoceros extinct and presently 2 species of amphibians, 14 species of reptiles 25 species of birds and 5 species of mammals are considered as endangered species. This paper is to produce a new assessment of the mangroves ecology of Sundarbans. DOI: http://dx.doi.org/10.3329/jsf.v8i1-2.14618 J. Sci. Foundation, 8(1&2): 35-47, June-December 2010

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