Correction to: New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

Abstract Background Barley (Hordeum vulgare L.) is one of the most important cereals worldwide. Although this crop is drought-tolerant, water deficiency negatively affects its growth and production. To detect key genes involved in drought tolerance in barley, a reconstruction of the related gene network and discovery of the hub genes would help. Here, drought-responsive genes in barley were collected through analysis of the available microarray datasets (− 5 ≥ Fold change ≥ 5, adjusted p value ≤ 0.05). Protein-protein interaction (PPI) networks were reconstructed. Results The hub genes were identified by Cytoscape software using three Cyto-hubba algorithms (Degree, Closeness, and MNC), leading to the identification of 17 and 16 non-redundant genes at vegetative and reproductive stages, respectively. These genes consist of some transcription factors such as HvVp1, HvERF4, HvFUS3, HvCBF6, DRF1.3, HvNAC6, HvCO5, and HvWRKY42, which belong to AP2, NAC, Zinc-finger, and WRKY families. In addition, the expression pattern of four hub genes was compared between the two studied cultivars, i.e., “Yousef” (drought-tolerant) and “Morocco” (susceptible). The results of real-time PCR revealed that the expression patterns corresponded well with those determined by the microarray. Also, promoter analysis revealed that some TF families, including AP2, NAC, Trihelix, MYB, and one modular (composed of two HD-ZIP TFs), had a binding site in 85% of promoters of the drought-responsive genes and of the hub genes in barley. Conclusions The identified hub genes, especially those from AP2 and NAC families, might be among key TFs that regulate drought-stress response in barley and are suggested as promising candidate genes for further functional analysis.

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Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

Plants ◽

10.3390/plants10091878 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1878

Author(s):

Kristýna Kundrátová ◽

Martin Bartas ◽

Petr Pečinka ◽

Ondřej Hejna ◽

Andrea Rychlá ◽

...

Keyword(s):

Drought Stress ◽

Stress Response ◽

Stress Responses ◽

Proteomic Analysis ◽

Large Scale ◽

Crop Yields ◽

Opium Poppy ◽

Metabolic Pathway Analysis ◽

Ubiquitin E3 Ligase ◽

Key Genes

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

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Key genes mining of DNA methylation involved in regulating drought stress response in potato

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2021.04152 ◽

2020 ◽

Vol 47 (4) ◽

pp. 599-612

Author(s):

Peng-Cheng LI ◽

Zhen-Zhen BI ◽

Chao SUN ◽

Tian-Yuan QIN ◽

Wen-Jun LIANG ◽

...

Keyword(s):

Dna Methylation ◽

Drought Stress ◽

Stress Response ◽

Key Genes

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Gene networks involved in drought stress response and tolerance

Journal of Experimental Botany ◽

10.1093/jxb/erl164 ◽

2006 ◽

Vol 58 (2) ◽

pp. 221-227 ◽

Cited By ~ 1349

Author(s):

K. Shinozaki ◽

K. Yamaguchi-Shinozaki

Keyword(s):

Drought Stress ◽

Stress Response ◽

Gene Networks

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Effects of maize organ-specific drought stress response on yields from transcriptome analysis

BMC Plant Biology ◽

10.1186/s12870-019-1941-5 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 9

Author(s):

Baomei Wang ◽

Can Liu ◽

Dengfeng Zhang ◽

Chunmei He ◽

Juren Zhang ◽

...

Keyword(s):

Drought Stress ◽

Stress Response ◽

Transcriptome Analysis ◽

Organ Specific

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Analysis of Populus glycosyl hydrolase family I members and their potential role in the ABA treatment and drought stress response

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2021.03.057 ◽

2021 ◽

Author(s):

Zhan Bian ◽

Dongli Wang ◽

Yunshan Liu ◽

Yimin Xi ◽

Xiaoling Wang ◽

...

Keyword(s):

Drought Stress ◽

Stress Response ◽

Potential Role ◽

Glycosyl Hydrolase ◽

Glycosyl Hydrolase Family ◽

Hydrolase Family

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Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-021-02040-3 ◽

2021 ◽

Author(s):

Ai-Hua Wang ◽

Lan Yang ◽

Xin-Zhuan Yao ◽

Xiao-Peng Wen

Keyword(s):

Drought Stress ◽

Stress Response ◽

Transgenic Plants ◽

Drought Tolerance ◽

Transgenic Tobacco ◽

Sequence Similarity ◽

Amino Acid Sequence Similarity ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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NAC Transcription Factor PwNAC11 Activates ERD1 by Interaction with ABF3 and DREB2A to Enhance Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms22136952 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6952

Author(s):

Mingxin Yu ◽

Junling Liu ◽

Bingshuai Du ◽

Mengjuan Zhang ◽

Aibin Wang ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Stress Response ◽

Transcriptional Activation ◽

Dominant Role ◽

State Level ◽

Energy Conversion Efficiency ◽

Nac Transcription Factor ◽

Wild Plants ◽

Transgenic Lines

NAC (NAM, ATAF1/2, and CUC2) transcription factors are ubiquitously distributed in eukaryotes and play significant roles in stress response. However, the functional verifications of NACs in Picea (P.) wilsonii remain largely uncharacterized. Here, we identified the NAC transcription factor PwNAC11 as a mediator of drought stress, which was significantly upregulated in P. wilsonii under drought and abscisic acid (ABA) treatments. Yeast two-hybrid assays showed that both the full length and C-terminal of PwNAC11 had transcriptional activation activity and PwNAC11 protein cannot form a homodimer by itself. Subcellular observation demonstrated that PwNAC11 protein was located in nucleus. The overexpression of PwNAC11 in Arabidopsis obviously improved the tolerance to drought stress but delayed flowering time under nonstress conditions. The steady-state level of antioxidant enzymes’ activities and light energy conversion efficiency were significantly increased in PwNAC11 transgenic lines under dehydration compared to wild plants. PwNAC11 transgenic lines showed hypersensitivity to ABA and PwNAC11 activated the expression of the downstream gene ERD1 by binding to ABA-responsive elements (ABREs) instead of drought-responsive elements (DREs). Genetic evidence demonstrated that PwNAC11 physically interacted with an ABA-induced protein—ABRE Binding Factor3 (ABF3)—and promoted the activation of ERD1 promoter, which implied an ABA-dependent signaling cascade controlled by PwNAC11. In addition, qRT-PCR and yeast assays showed that an ABA-independent gene—DREB2A—was also probably involved in PwNAC11-mediated drought stress response. Taken together, our results provide the evidence that PwNAC11 plays a dominant role in plants positively responding to early drought stress and ABF3 and DREB2A synergistically regulate the expression of ERD1.

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