Correlation of lead and cadmium in human seminal plasma with seminal vesicle and prostatic markers

2003 ◽  
Vol 22 (3) ◽  
pp. 125-128 ◽  
Author(s):  
N Pant ◽  
A K Banerjee ◽  
S Pandey ◽  
N Mathur ◽  
D K Saxena ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Seminal Vesicle ◽  
Seminal Plasma ◽  
Biochemical Markers ◽  
Semen Quality ◽  
Human Seminal Plasma ◽  
Accessory Sex Glands ◽  
Lead And Cadmium ◽  
Glutamyl Transpeptidase

The objective of this study was to investigate the correlation between lead and cadmium with seminal vesicle and prostatic markers. Semen samples categorized into fertile and infertile were evaluated for the presence of lead and cadmium and biochemical markers in the seminal plasma. Associations between lead and fructose, acid phosphatase and g-glutamyl transpeptidase (g-GT) were observed. However, no such relationships were noticed for cadmium. It is concluded that lead may be one of the pollutants indirectly affecting semen quality by altering the functions of accessory sex glands.

Use of biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)

2014 ◽  
Vol 17 (3) ◽  
pp. 427-432 ◽  
Author(s):  
K. Stasiak ◽  
J. Glogowski ◽  
W. Demianowicz ◽  
R. Kowalski ◽  
A. Nowak-Tkaczyk ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Seminal Plasma ◽  
Biochemical Markers ◽  
Semen Quality ◽  
The Arctic ◽  
Arctic Fox ◽  
Vulpes Lagopus

Abstract The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh - measurement A; equilibrated - measurement B; frozen/thawed - measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm3, and ejaculate volume averaged 1.6 cm3. Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×103 U/l) and lowest activity of acrosin inhibitors (4.55×103 U/l). After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.

Simple, rapid determination of zinc and acid phosphatase in seminal plasma with an ABA-100 bichromatic analyzer.

1988 ◽  
Vol 34 (8) ◽  
pp. 1605-1607 ◽  
Author(s):  
M Gavella
Keyword(s):  
Acid Phosphatase ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Rapid Determination ◽  
Assay System ◽  
Human Seminal Plasma ◽  
Automated Assay ◽  
Sensitivity Specificity

Abstract I describe an automated assay for zinc and acid phosphatase in seminal plasma. These, which are markers of the function of the prostate, were assayed bichromatically with an Abbott ABA-100 analyzer. As many as 25 samples of human seminal plasma can be analyzed sequentially with CVs of 3.1% for zinc and 1.5% for acid phosphatase. The sensitivity, specificity, and speed of this assay system make it practicable for use in investigation of male infertility.

scholarly journals Prostate Secretory Protein of 94 Amino Acids (PSP94) Binds to Prostatic Acid Phosphatase (PAP) in Human Seminal Plasma

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58631 ◽  
Author(s):  
Jenifer H. Anklesaria ◽  
Dhanashree D. Jagtap ◽  
Bhakti R. Pathak ◽  
Kaushiki M. Kadam ◽  
Shaini Joseph ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acid Phosphatase ◽  
Seminal Plasma ◽  
Prostatic Acid Phosphatase ◽  
Secretory Protein ◽  
Human Seminal Plasma

scholarly journals Human seminal plasma contains somatostatin-64

1994 ◽  
Vol 303 (1) ◽  
pp. 263-268 ◽  
Author(s):  
L Odum ◽  
A H Johnsen
Keyword(s):  
Seminal Plasma ◽  
Biologically Active ◽  
Gel Chromatography ◽  
Human Seminal Plasma ◽  
Active Peptides ◽  
Accessory Sex Glands ◽  
Biologically Active Peptides ◽  
Terminal Amino ◽  
High Concentrations ◽  
Normal Men

Prosomatostatin is processed into two biologically active peptides, somatostatin (SS)-14 and SS-28. SS-14 is found in many tissues, whereas SS-28 is most prominent in the intestine. Human seminal plasma contained high concentrations (median = 3 nmol/l, range 0.6-76 nmol/l, n = 17) of SS with a higher M(r) than that of SS-28 as determined by gel chromatography. After isolation the SS immunoreactivity was identified as SS-64 based on the N-terminal amino acid sequence Leu-Ala-Glu-Leu-Leu-Ser-Glu-Pro-Asn-Gln-, and M(r) of 7242 determined by m.s. and on reactivity with two antibodies raised against SS-14. SS-64 displaced the binding of [125I-Tyr1]SS-14 to rat cerebrocortical membranes as effectively as SS-14 (IC50 = 1.6 mol/l). The concentration of SS-64 in seminal plasma from vasectomized men was significant higher (P < 0.005) than in normal men (median = 25.4 nmol/l, range 1.5-156 nmol/l, n = 21), suggesting that the synthesis of SS-64 takes place in the male accessory sex glands.

scholarly journals Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality

2020 ◽  
Author(s):  
Zhanhui Ou ◽  
Qirong Wen ◽  
Yu Deng ◽  
Yang Yu ◽  
Zhiheng Chen ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Semen Quality ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Cigarette Smoke Condensate ◽  
Human Seminal Plasma ◽  
High Level ◽  
Heavy Smokers

Abstract Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet. Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level) were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC). Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P<0.05). Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.

Andrology: Diamine oxidase activity and biochemical markers in human seminal plasma

1995 ◽  
Vol 10 (5) ◽  
pp. 1141-1144 ◽  
Author(s):  
M. Le Calvé ◽  
J. Segalen ◽  
D. Quernee ◽  
M.T. Lavault ◽  
D. Lescoat
Keyword(s):  
Seminal Plasma ◽  
Oxidase Activity ◽  
Biochemical Markers ◽  
Diamine Oxidase ◽  
Human Seminal Plasma ◽  
Diamine Oxidase Activity

scholarly journals Distribution of hyaluronan in bull reproductive organs.

1994 ◽  
Vol 42 (11) ◽  
pp. 1479-1486 ◽  
Author(s):  
R Tammi ◽  
S Rönkkö ◽  
U M Agren ◽  
M Tammi
Keyword(s):  
Seminal Vesicle ◽  
Seminal Plasma ◽  
Reproductive Tract ◽  
Reproductive Organs ◽  
Accessory Gland ◽  
Seminiferous Tubules ◽  
Tissue Sections ◽  
Accessory Sex Glands ◽  
Cartilage Proteoglycan

To study the expression of hyaluronan in male reproductive organs and the origin of seminal plasma hyaluronan, we stained various parts of the bull reproductive tract for hyaluronan using a biotinylated probe derived from cartilage proteoglycan (bHABC). The potential loss of hyaluronan during tissue processing was checked with a novel technique by blotting frozen tissue sections on nitrocellulose and staining the blots with bHABC. In the same tissues the CD44 receptor was visualized by Hermes 1 antibody. The testes showed only traces of hyaluronan, whereas both the epithelium and the connective tissue of seminal vesicle, prostate, Cowper's gland, and epididymis were positive in bHABC staining. Hyaluronan was localized on the basolateral surfaces of these epithelial cells. The secretions inside the seminal vesicle and in the ducts of prostate and Cowper's gland were HA-positive, whereas the luminal contents of seminiferous tubules and epididymis were unstained both in paraffin sections and in the in situ blocks. The data indicate that hyaluronan in seminal plasma originates from the accessory sex glands. The co-localization of CD44 with hyaluronan in the basolateral surfaces of the accessory gland epithelia and its absence from other epithelia with little or no hyaluronan supports its role as a hyaluronan receptor.

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