Pre and postnatal exposure to endosulfan in Wistar rats

The possible reproductive adverse effects of the pesticide endosulfan on male offspring rats exposed in utero and during lactation were investigated. Dams were treated orally with 0, 0.5 or 1.5 mg of endosulfan/kg 21 days prior to mating, during the mating, pregnancy and lactation. Maternal and reproductive outcome data and male sexual development landmarks (testis descent and preputial separation) were assessed. Reproductive endpoints of the male offspring were examined at adulthood: sex organ weights, daily sperm production, spermatid number, sperm transit, sperm morphology and testosterone level. No signs of maternal toxicity were detected at the dose levels tested. Sexual development landmarks were also unaffected. Moreover, with the exception of a significant increase in the relative epididymis weight seen in the group treated with the lowest dose, we have not found any statistically significant adverse effect in the reproductive endpoints investigated at adulthood. The results of the present study indicate that pre and post-natal exposure to low doses of endosulfan (0.5 and 1.5 mg/kg) do not induce significant adverse effects in the reproductive system of male offspring Wistar rats at adulthood.

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Reproductive effects of endosulfan on male offspring of rats exposed during pregnancy and lactation

Human & Experimental Toxicology ◽

10.1191/096032799678845124 ◽

1999 ◽

Vol 18 (9) ◽

pp. 583-589 ◽

Cited By ~ 38

Author(s):

P R Dalsenter ◽

E Dallegrave ◽

J Rb Mello ◽

A Langeloh ◽

R T Oliveira ◽

...

Keyword(s):

Male Offspring ◽

Male Rats ◽

Male Rat ◽

Seminiferous Tubules ◽

Sperm Production ◽

Rat Offspring ◽

Stage Of Development ◽

Reproductive Effects ◽

Pregnancy And Lactation ◽

Daily Sperm Production

1 The reproductive effects of endosulfan on the male offspring of rats were examined. Dams were treated orally with 0, 1.5 or 3.0 mg endosulfan/kg from day 15 of pregnancy to postnatal day (PND) 21 of lactation. The male offspring rats were investigated at PND 65 or 140, corresponding to the pubertal and adulthood stage of development. 2 The dose of 3.0 mg endosulfan/kg induced a decrease in maternal body weight during pregnancy, but litter size and mean birth weight were not affected. Similarly, the age at testis descent and preputial separation was not affected on the male offspring. 3 The daily sperm production (6106) was permanently decreased in the highest dose group when investigated at puberty and at adulthood. At the lowest dose, however, the daily sperm production was significantly reduced only at puberty. 4 Histologically, the percentage of seminiferous tubules showing complete spermatogenesis was significantly decreased at puberty. This finding may explain the decrease in daily sperm production observed in the endosulfan-exposed male rats. 5 The results of this study show that low doses of endosulfan have no apparent effect on developmental landmarks or on the weight of reproductive and accessory sex organ. Daily sperm production was the most susceptible endpoint in the male offspring exposed to endosulfan during pregnancy and lactation. To further understand the reproductive effects of endosulfan on male rat offspring, additional reproductive and toxicokinetic studies should be carried out to determine the extent of endosulfan exposure in male rat offspring in utero and during lactation.

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Adverse effects of prenatal and early postnatal exposure to antiepileptic drugs: Validation from clinical and basic researches

Brain and Development ◽

10.1016/j.braindev.2017.03.026 ◽

2017 ◽

Vol 39 (8) ◽

pp. 635-643 ◽

Cited By ~ 11

Author(s):

Kimino Fujimura ◽

Takayuki Mitsuhashi ◽

Takao Takahashi

Keyword(s):

Adverse Effects ◽

Antiepileptic Drugs ◽

Postnatal Exposure

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Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure

International Journal of Molecular Sciences ◽

10.3390/ijms22052674 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2674

Author(s):

Chien-Ning Hsu ◽

Julie Y. H. Chan ◽

Kay L. H. Wu ◽

Hong-Ren Yu ◽

Wei-Chia Lee ◽

...

Keyword(s):

Gut Microbiota ◽

Negative Impact ◽

Short Chain Fatty Acids ◽

Normal Diet ◽

Male Offspring ◽

Sprague Dawley ◽

Minocycline Treatment ◽

High Fructose ◽

Induced Hypertension ◽

Pregnancy And Lactation

Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF) diet-induced hypertension in adult offspring is relevant to altered gut microbiome and its metabolites. We, therefore, intended to examine whether minocycline administration during pregnancy and lactation may further affect blood pressure (BP) programmed by maternal HF intake via mediating gut microbiota and SCFAs. Pregnant Sprague-Dawley rats received a normal diet or diet containing 60% fructose throughout pregnancy and lactation periods. Additionally, pregnant dams received minocycline (50 mg/kg/day) via oral gavage or a vehicle during pregnancy and lactation periods. Four groups of male offspring were studied (n = 8 per group): normal diet (ND), high-fructose diet (HF), normal diet + minocycline (NDM), and HF + minocycline (HFM). Male offspring were killed at 12 weeks of age. We observed that the HF diet and minocycline administration, both individually and together, causes the elevation of BP in adult male offspring, while there is no synergistic effect between them. Four groups displayed distinct enterotypes. Minocycline treatment leads to an increase in the F/B ratio, but decreased abundance of genera Lactobacillus, Ruminococcus, and Odoribacter. Additionally, minocycline treatment decreases plasma acetic acid and butyric acid levels. Hypertension programmed by maternal HF diet plus minocycline exposure is related to the increased expression of several SCFA receptors. Moreover, minocycline- and HF-induced hypertension, individually or together, is associated with the aberrant activation of the renin–angiotensin system (RAS). Conclusively, our results provide a new insight into the support of gut microbiota and its metabolite SCAFs in the developmental programming of hypertension and cast new light on the role of RAS in this process, which will help prevent hypertension programmed by maternal high-fructose and antibiotic exposure.

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Are Probiotics and Prebiotics Safe for Use during Pregnancy and Lactation? A Systematic Review and Meta-Analysis

Nutrients ◽

10.3390/nu13072382 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2382

Author(s):

Hauna Sheyholislami ◽

Kristin L. Connor

Keyword(s):

Systematic Review ◽

Adverse Effects ◽

English Language ◽

Clinical Decision Making ◽

Lactobacillus Rhamnosus ◽

Potential Health ◽

Increased Risk ◽

Pregnancy And Lactation ◽

Adverse Pregnancy ◽

Synbiotic Supplementation

Probiotic and prebiotic products have shown potential health benefits, including for the prevention of adverse pregnancy outcomes. The incidence of adverse effects in pregnant people and their infants associated with probiotic/prebiotic/synbiotic intake, however, remains unclear. The objectives of this study were to evaluate the evidence on adverse effects of maternal probiotic, prebiotic, and/or synbiotic supplementation during pregnancy and lactation and interpret the findings to help inform clinical decision-making and care of this population. A systematic review was conducted following PRISMA guidelines. Scientific databases were searched using pre-determined terms, and risk of bias assessments were conducted to determine study quality. Inclusion criteria were English language studies, human studies, access to full-text, and probiotic/prebiotic/synbiotic supplementation to the mother and not the infant. In total, 11/100 eligible studies reported adverse effects and were eligible for inclusion in quantitative analysis, and data were visualised in a GOfER diagram. Probiotic and prebiotic products are safe for use during pregnancy and lactation. One study reported increased risk of vaginal discharge and changes in stool consistency (relative risk [95% CI]: 3.67 [1.04, 13.0]) when administering Lactobacillus rhamnosus and L. reuteri. Adverse effects associated with probiotic and prebiotic use do not pose any serious health concerns to mother or infant. Our findings and knowledge translation visualisations provide healthcare professionals and consumers with information to make evidence-informed decisions about the use of pre- and probiotics.

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Effects of Perinatal Simultaneous Exposure to Tributyltin (TBT) andp, p′-DDE (1,1-Dichloro-2,2-Bis(p-Chlorophenyl) Ethylene) on Male Offspring of Wistar Rats

Journal of Toxicology and Environmental Health Part A ◽

10.1080/15287390490273451 ◽

2004 ◽

Vol 67 (5) ◽

pp. 385-395 ◽

Cited By ~ 9

Author(s):

Yuji Makita ◽

Minoru Omura ◽

Rika Ogata

Keyword(s):

Wistar Rats ◽

Male Offspring ◽

Simultaneous Exposure

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Evaluation of Anti-fertility activity of Decaschistia crotonifolia leaves on female wistar rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i3.1451 ◽

2019 ◽

Vol 10 (3) ◽

pp. 2194-2198

Author(s):

Afsar Shaik ◽

Prasanna Raju Yalavarthi ◽

Chadrasekhar Kothapalli Bonnoth

Keyword(s):

Medicinal Plants ◽

Wistar Rats ◽

Uterine Weight ◽

Estrogenic Effect ◽

Different Types ◽

Female Wistar Rats ◽

Fertility Potential ◽

Dose Levels ◽

Modern Era ◽

Ovariectomised Rats

Medicinal Plants were used from the ancient to the modern era and has proved in treating and preventing many different types of diseases which are not treatable with other means of treatment. The present study was aimed to prove the anti-fertility activity of Decaschistia crotonifolia leaves on female wistar rats. The extracts were mainly estimated for their anti-implantation activity by taking mainly 2 dose levels: 200 & 400 mg/kg, respectively. The extracts were also tested for their hormonal alteration effects on female wistar rats. The reports obtained in this study strongly prove the anti-fertility potential of leaves extracts of Decaschistia crotonifolia, as the extracts has shown a potential decline in the formation of implants (100%), and also the increase in uterine weight projects its estrogenic effect in Ovariectomised rats. Hence by considering the above-mentioned results, it may be proved that the leaves extracts of D. Crotonifolia poccess strong anti-fertility activity.

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Linking the response of endocrine regulated genes to adverse effects on sex differentiation improves comprehension of aromatase inhibition in a Fish Sexual Development Test

Aquatic Toxicology ◽

10.1016/j.aquatox.2016.04.018 ◽

2016 ◽

Vol 176 ◽

pp. 116-127 ◽

Cited By ~ 16

Author(s):

Elke Muth-Köhne ◽

Kathi Westphal-Settele ◽

Jasmin Brückner ◽

Sabine Konradi ◽

Viktoria Schiller ◽

...

Keyword(s):

Adverse Effects ◽

Sexual Development ◽

Sex Differentiation ◽

Aromatase Inhibition

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Increased exposure to sodium during pregnancy and lactation changes basal and induced behavioral and neuroendocrine responses in adult male offspring

Physiological Reports ◽

10.14814/phy2.13210 ◽

2017 ◽

Vol 5 (6) ◽

pp. e13210 ◽

Cited By ~ 4

Author(s):

Marcia S. Silva ◽

Fabiana Lúcio-Oliveira ◽

Andre Souza Mecawi ◽

Lucas F. Almeida ◽

Silvia G. Ruginsk ◽

...

Keyword(s):

Adult Male ◽

Male Offspring ◽

Pregnancy And Lactation ◽

Neuroendocrine Responses

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Transplacental Toxicity and Carcinogenesis Studies in Rats with Hexamethylenetetramine

Tumori Journal ◽

10.1177/030089167005600602 ◽

1970 ◽

Vol 56 (6) ◽

pp. 325-334 ◽

Cited By ~ 16

Author(s):

Giuseppe Della Porta ◽

José R. Cabral ◽

Giorgio Parmiani

Keyword(s):

Drinking Water ◽

Body Weight ◽

Wistar Rats ◽

The Body ◽

Carcinogenic Activity ◽

Long Term Experiments ◽

Pregnancy And Lactation ◽

Successive Generations ◽

And Control

In a previous paper (Fd Cosmet. Toxicol., 6: 707–715, 1968) it was reported that hexamethylenetetramine (HMT) had no carcinogenic activity in long-term experiments in mice and rats. In the present study, 12 ♀ and 6 ♂ Wistar rats were given 1% HMT in the drinking water starting 2 weeks before mating. The females were kept under treatment during pregnancy and lactation. A similar untreated group of 12 ♀ and 6 ♂ served as control. Twelve treated females and eleven controls became pregnant and gave birth to 124 and 118 babies respectively; no malformations were noted. From these animals, 24 for each sex were continued on the 1% HMT up to the 20th week of age or were kept untreated. The body weight of treated animals was significantly lower than that of controls one, only up to the 9th week of age for the males and up to the 13th week for the females. At the end of the treatment both groups were sacrificed; the weight of organs was identical in the treated and control animals; there were no gross or histological pathology. In a second experiment, rats were given 1% HMT in the drinking water for 3 successive generations, up to the age of 40 weeks in the F1 and F2 groups and of 20 weeks for F3. The three groups were composed of 13 ♂ and 7 ♀, 15 ♂ and 11 ♀, 12 ♂ and 12 ♂, respectively. In addition, a group of 16 ♂ and 16 ♀ descendants of 2% HMT treated parents, were given 2% HMT for 50 weeks. A group of 48 ♂ and 48 ♀ served as untreated controls. All groups were kept under observation for over 2 years of age. No evidence of carcinogenicity was found in any of the HMT-treated groups.

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