Concordance of local and central laboratory hormone and HER2 receptor status in ECOG 2197

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 21022-21022 ◽  
Author(s):  
S. S. Badve ◽  
F. L. Baehner ◽  
R. Gray ◽  
B. Childs ◽  
T. Maddala ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Pcr Analysis ◽  
Central Laboratory ◽  
Rt Pcr ◽  
Her2 Receptor ◽  
High Incidence ◽  
High Degree ◽  
The Relationship ◽  
Very High ◽  
Local Laboratory

21022 Background: Central and local laboratory concordance for hormone and HER2 receptor measurement is of national interest. This study compares ER/PR/HER2 by local laboratories using immunohistochemistry (IHC) and central laboratories (IHC & quantitative RT-PCR). Methods: Of 2952 patients in E2197, a case-cohort sample of 776 patients who either did (N=179) or did not recur was studied. Central IHC for ER/PR/HER2 was performed using single 0.6 mm microarrays; Allred score (AS) was used for ER/PR (AS>2 = positive). Positive HER2 was 3+ staining in >10% cells for Central IHC and 2+ or 3+ for Local IHC. RT-PCR analysis by Oncotype DX™ for ER/PR/HER2 was performed using pre-defined cutoffs of 6.5, 5.5 and 11.5 units, respectively. Hormone receptor (HR) pos was defined as ER &/or PR pos. Results: Results from Local IHC (ER/PR in 776 & HER2 in 517 pts) were compared with Central IHC (760 pts) and RT-PCR results (776 pts). The discordance between HR positivity by Local IHC and RT-PCR was very low. However, 12% of HR neg pts by Local IHC (38/321) & Central IHC (39/326) were HR pos by RT-PCR. The relationship between ER and recurrence as a function of AS was examined. Patients with AS of 3–4 were found to be closer to the AS=2 group than to the AS>4 group Patients with AS of 3–4 were found to be closer to the AS ÿ 2 group than to the AS > 4 group (Est.HR for ER 0.97 for AS 3–4 vs. 0–2 and 0.46 for AS 5–8 vs. 0–2, and for PR were 0.84 for AS 3–4 vs. 0–2 and 0.41 for AS 5–8 vs. 0–2). Conclusions: There is a high degree of overall concordance among Local IHC, Central IHC, and Central RT-PCR for ER and PR. The degree of concordance is even greater for HR compared to ER or PR alone. Although the concordance with local labs for HER2 testing was poor, the concordance between Central IHC and RT-PCR was very high. The relatively high incidence (12%) of IHC HR neg pts who are HR pos by RT- PCR is notable. [Table: see text] [Table: see text]

scholarly journals Quantitative measurement of infectious virus in SARS-CoV-2 Alpha, Delta and Epsilon variants reveals higher infectivity (viral titer:RNA ratio) in clinical samples containing the Delta and Epsilon variants.

2021 ◽  
Author(s):  
Hannah W Despres ◽  
Margaret G Mills ◽  
David J Shirley ◽  
Madaline M Schmidt ◽  
Meei-Li Huang ◽  
...  
Keyword(s):  
Quantitative Measurement ◽  
Infectious Virus ◽  
Viral Rna ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Genome Copy ◽  
Live Virus ◽  
High Degree ◽  
The Relationship ◽  
Rna Levels

ABSTRACT Background Novel SARS-CoV-2 Variants of Concern (VoC) pose a challenge to controlling the COVID-19 pandemic. Previous studies indicate that clinical samples collected from individuals infected with the Delta variant may contain higher levels of RNA than previous variants, but the relationship between viral RNA and infectious virus for individual variants is unknown. Methods We measured infectious viral titer (using a micro-focus forming assay) as well as total and subgenomic viral RNA levels (using RT-PCR) in a set of 165 clinical samples containing SARS-CoV-2 Alpha, Delta and Epsilon variants that were processed within two days of collection from the patient. Results We observed a high degree of variation in the relationship between viral titers and RNA levels. Despite the variability we observed for individual samples the overall infectivity differed among the three variants. Both Delta and Epsilon had significantly higher infectivity than Alpha, as measured by the number of infectious units per quantity of viral E gene RNA (6 and 4 times as much, p=0.0002 and 0.009 respectively) or subgenomic E RNA (11 and 7 times as much, p<0.0001 and 0.006 respectively). Conclusion In addition to higher viral RNA levels reported for the Delta variant, the infectivity (amount of replication competent virus per viral genome copy) may also be increased compared to Alpha. Measuring the relationship between live virus and viral RNA is an important step in assessing the infectivity of novel SARS-CoV-2 variants. An increase in the infectivity of the Delta variant may further explain increased spread and suggests a need for increased measures to prevent viral transmission.

scholarly journals Novel Expression of Type 1 Corticotropin-Releasing Hormone Receptor in Multiple Endocrine Cell Types in the Murine Anterior Pituitary

Endocrinology ◽  
2008 ◽  
Vol 150 (1) ◽  
pp. 260-267 ◽  
Author(s):  
Nicole J. Westphal ◽  
Ryan T. Evans ◽  
Audrey F. Seasholtz
Keyword(s):  
Hormone Receptor ◽  
Anterior Pituitary ◽  
Cell Types ◽  
Pituitary Cell ◽  
Pcr Analysis ◽  
Corticotropin Releasing Hormone ◽  
Rt Pcr ◽  
Releasing Hormone ◽  
Anterior Pituitary Cell

The CRH family of ligands signals via two distinct receptors, CRH-R1 and CRH-R2. Previous studies localized CRH-R1 and CRH-R2 to a subset of anterior pituitary corticotropes and gonadotropes, respectively. However, numerous studies have indicated that stress and CRH activity can alter the secretion of multiple anterior pituitary hormones, suggesting a broader expression of the CRH receptors in pituitary. To examine this hypothesis, the in vivo expression of CRH-R1 and CRH-R2 mRNA was further characterized in adult mouse pituitary. Quantitative RT-PCR analysis demonstrated that CRH-R1 mRNA is greater than 100-fold more abundant than CRH-R2 mRNA in male and female mouse pituitaries. Dual in situ hybridization analysis identified cell-specific CRH-R1 expression in the anterior pituitary. At least half of the CRH-R1-positive cells expressed proopiomelanocortin-mRNA (50% in females; 70% in males). In females, a significant percentage of the cells expressing CRH-R1 also expressed transcript for prolactin (40%), LHβ (10%), or TSH (3%), all novel sites of CRH-R1 expression. Similarly in males, a percentage of CRH-R1-positive cells expressed prolactin (12%), LHβ (13%), and TSH (5%). RT-PCR studies with immortalized murine anterior pituitary cell lines showed CRH-R1 and/or CRH-R2 expression in corticotropes (AtT-20 cells), gonadotropes (αT3-1 and LβT2 cells), and thyrotropes (αTSH cells). Whereas CRH-R1 expression in corticotropes is well established, the presence of CRH-R1 mRNA in a subset of lactotropes, gonadotropes, and thyrotropes establishes these cell types as novel sites of murine CRH-R1 expression and highlights the pituitary as an important site of interaction between the hypothalamus-pituitary-adrenal and multiple endocrine axes. Corticotropin-releasing hormone receptor 1 mRNA is detected in multiple mouse anterior pituitary cell types, including corticotropes, lactotropes, and gonadotropes, in a sexually dimorphic pattern.

scholarly journals Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia

2019 ◽  
Vol 17 (1) ◽  
pp. e1004 ◽  
Author(s):  
Darko Jevremović ◽  
Aleksandar Leposavić ◽  
Svetlana A Paunović
Keyword(s):  
Phylogenetic Analysis ◽  
Large Scale ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Leaf Blotch ◽  
High Incidence ◽  
Long Time ◽  
Large Scale Survey ◽  
Polymerase Chain ◽  
Degree Of Association

Raspberry leaf blotch emaravirus (RLBV) is a recently characterised virus infecting raspberries reported in several European countries. RLBV causes yellow blotching, the distortion of leaf margins, and the twisting of raspberry leaves. For a long time, similar symptoms were attributed to the feeding damage caused by raspberry leaf and bud mite (Phyllocoptes gracilis). From 2014−2017, a large-scale survey was conducted in Serbia to investigate the degree of association of the observed symptoms with the RLBV infection. A total of 98 symptomatic and asymptomatic samples were collected from 30 locations. All collected samples were tested on the RLBV presence by reverse transcription and polymerase chain reaction (RT-PCR) using three sets of RNA-specific primers targeting RNA-1, RNA-3, and RNA-5 of the RLBV genome. RT-PCR analysis revealed high incidence of RLBV in tested samples (68.7%). RLBV was confirmed in raspberries ‘Fertödi Zamatos’, ‘Glen Ample’, ‘Meeker’, ‘Polana’, ‘Tulameen’ and ‘Willamette’. Twenty-one isolates were selected for sequencing the portion of the nucleocapsid (NC) gene. The nucleotide sequences of the isolates showed 93.2−100% identity. Phylogenetic analysis confirmed significant genetic variability of the Serbian RLBV isolates based on the nucleocapsid-encoding sequences and revealed the existence of two main clusters. Phylogenetic analysis of the 45 RLBV sequences from Finland, Slovakia, Scotland, and this study also confirmed the existence of two main clusters of isolates.

scholarly journals Hubungan Tingkat Kejadian Karies Gigi Dengan Status Gizi Anak Usia 6 -7 Tahun Di SD Inpres Kaniti Kecamatan Kupang Tengah Kabupaten Kupang

2018 ◽  
Vol 16 (1) ◽  
pp. 32-43
Author(s):  
Ferdinan Fankari
Keyword(s):  
Dental Caries ◽  
Nutritional Status ◽  
Household Level ◽  
Oral Health Problem ◽  
High Incidence ◽  
Quality Of Health Services ◽  
Nutritional Status Of Children ◽  
The Relationship ◽  
Very High

Dental caries is a dental and oral health problem for the majority of the Indonesian population. Dental caries affects most children. Caries that occur in children is often not treated or treated, resulting in a toothache. This condition can have an impact on the general health of the child where the frequency of eating will decrease, resulting in disruption of growth and development that will affect the nutritional status of the child. Nutritional problems are caused by many factors that are interrelated both directly and indirectly. Directly affected by infectious diseases and insufficient nutrition in quantity and quality, while indirectly influenced by the range and quality of health services, inadequate parenting, poor sanitation conditions and low food security at the household level. This study aims to determine the relationship between the incidence of dental caries in children with the nutritional status of children aged 6-7 years SDI Kaniti Kabupaten Kupang in 2016. Data collection was carried out by performing dental examinations and measuring height and weight. The incidence rate of dental caries aged 6-7 years old SDI Kaniti Kabupaten Kupang in 2016 was in the high category (30.76%) and very high (37.17%) with an average 3-6 cavities and nutritional status of children aged 6- 7 years SDN Kaniti Kabupaten Kupang in 2016 was categorized as obese (26.92%) and normal (67.94%), so there was no correlation between the incidence of dental caries on the nutritional status of 6-7-year-olds in Kaniti Regency Kupang in 2016 because children who had high and very high caries incidence has fat and normal nutritional status. It is recommended to conduct further research on the factors that influence the high incidence of dental caries in children aged 6-7 years at SDN Kaniti, Kupang Tengah District, Kupang Regency.

Jassid Resistance and Hairiness of the Cotton Plant

1949 ◽  
Vol 39 (4) ◽  
pp. 539-575 ◽  
Author(s):  
F. R. Parnell ◽  
H. E. King ◽  
D. F. Ruston
Keyword(s):  
Cotton Plant ◽  
Visual Assessment ◽  
Stages Of Growth ◽  
Wide Range ◽  
Leaf Hairiness ◽  
Resistant Strains ◽  
High Degree ◽  
The Relationship ◽  
Very High ◽  
Continuous Range

A wide range of material has been studied in an investigation of the relationship between “resistance” to attack by Empoasca facialis and “hairiness” of the cotton plant.A continuous range has been found between full susceptibility and very high resistance.In general relative susceptibility, as gauged by visual assessment of symptoms, has been found to correspond closely with relative infestation, determined by counts of nymphs.With attention confined to grown plants, the relative susceptibility of varieties has been found substantially constant under varying levels of exposure to infestation.Hairiness of the cotton plant has been found to show an extremely wide range in degree, style and distribution. A method has been devised for expressing hairiness numerically, taking into account both length and density of hairs.A very close and consistent relationship has been found between degree of hairiness of the under surface of the leaf, and degree of resistance to Jassid. Without exception, all thoroughly hairy types have been found highly resistant, and all non-hairy types fully susceptible. Intermediate degrees of hairiness ate associated with intermediate degrees of resistance.The relationship has been found to hold good between varieties and between plants within varieties, of the species G. hirsutum; also between plants of G. barbadense, and in segregating progenies of hybrids between these two species.The hairiness of resistant strains of cotton has been found to develop gradually with the growth of the plant; the first few leaves on the seedlings being virtually non-hairy. This lack of hairiness in the early stages of growth is associated with a lack of resistance. Hairiness and resistance to Jassid develop concurrently.The conclusion is reached that hairiness of the leaf confers resistance to Jassid and that degree of leaf hairiness, measured in an appropriate manner, is a thoroughly reliable guide to degree of resistance.Length of hairs is shown to be of prime importance, and high densities without adequate length are ineffective.The relative importance of hairs on lamina and midrib has not been conclusively determined. Both have an influence on resistance but a high degree of midrib hairiness is not essential if the lamina is hairy.Hairs on stem and petiole are shown to be of very little direct importance.

Hormone receptor discordance between local and central pathology with RT-PCR analysis: Results from multicenter Phase III WSG-PlanB trial.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e11555-e11555
Author(s):  
Raquel Von Schumann ◽  
Oleg Gluz ◽  
Ulrike Nitz ◽  
Michael Clemens ◽  
Kristina Luebbe ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Phase Iii ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Multicenter Phase ◽  
Central Pathology

scholarly journals Countrywide Spread of CTX-M-3 Extended-Spectrum β-Lactamase-Producing Microorganisms of the Family Enterobacteriaceae in Poland

2002 ◽  
Vol 46 (1) ◽  
pp. 151-159 ◽  
Author(s):  
Anna Baraniak ◽  
Janusz Fiett ◽  
Agnieszka Sulikowska ◽  
Waleria Hryniewicz ◽  
Marek Gniadkowski
Keyword(s):  
Pcr Analysis ◽  
Randomly Amplified Polymorphic Dna ◽  
In Vitro Bioassay ◽  
Extended Spectrum ◽  
The Family ◽  
Dna Types ◽  
High Degree ◽  
Very High ◽  
Permeability Alterations

ABSTRACT Eighty-four clinical isolates of the family Enterobacteriaceae, recovered from 1998 to 2000 in 15 hospitals in 10 Polish cities, were analyzed. All the isolates produced β-lactamases with pIs of 8.4 and 5.4, and the pI 8.4 enzymes were demonstrated to hydrolyze cefotaxime but not ceftazidime in the in vitro bioassay. PCR analysis and DNA sequencing have revealed that in all cases the pI 8.4 β-lactamase was probably the CTX-M-3 extended-spectrum β-lactamase (ESBL) variant, which was originally identified in 1996 in Praski Hospital in Warsaw. In the majority of isolates, bla CTX-M-3 genes resided within large conjugative plasmids with similar fingerprints, which, in the context of the high degree of diversity of the randomly amplified polymorphic DNA types of the isolates, suggested that horizontal transfer of plasmids was likely the main mechanism of CTX-M-3 spread. The dissemination of plasmids was probably preceded by the center-to-center transmission of several strains, as indicated by the identification by pulsed-field gel electrophoresis of closely related or possibly related Klebsiella pneumoniae, Escherichia coli, and Citrobacter freundii isolates in five different hospitals. CTX-M-3-producing organisms revealed a very high degree of diversity in β-lactam resistance levels and patterns. This was attributed to several factors, such as the production of other β-lactamases including additional ESBLs, possible quantitative variations in CTX-M-3 expression, segregation of AmpC derepressed mutants, and permeability alterations.

scholarly journals A re-examination of ENSO/ anti-ENSO events and simultaneous performance of the Indian summer monsoon

MAUSAM ◽  
2021 ◽  
Vol 47 (1) ◽  
pp. 59-66
Author(s):  
J. CHATTOPADHYAY ◽  
R. BHATLA
Keyword(s):  
Monsoon Rainfall ◽  
Southern Oscillation ◽  
Monsoon Season ◽  
Strong Association ◽  
Enso Events ◽  
The Pacific ◽  
Degree Of Confidence ◽  
High Degree ◽  
The Relationship ◽  
Very High

The relationship between ENSO/anti-ENSO events in the Pacific basin and simultaneous all India monsoon has been re-examined for the period 1901-1990 using Southern Oscillation Index (SOI). The result shows that there is fairly strong association between ENSO events and dry monsoon years. There exists a weak teleconnection between anti-ENSO events and wet monsoon indicating that anti-ENSO events have only a moderate impact on the Indian monsoon rainfall. Developing ENSO (anti-ENSO) episodes during the monsoon season indicates non-occurrence of simultaneous floods (droughts) with a very high degree of confidence 70 (50) percent of the droughts (floods) during the above period have occurred during ENSO (anti-ENSO) events indicating that extreme monsoon activities in the form of droughts (floods) might be important factors for the occurrence of simultaneous ENSO/anti-ENSO events.

scholarly journals Role of Vfr in regulating exotoxin A production by Pseudomonas aeruginosa

Microbiology ◽  
2009 ◽  
Vol 155 (7) ◽  
pp. 2265-2273 ◽  
Author(s):  
Marko Davinic ◽  
Nancy L. Carty ◽  
Jane A. Colmer-Hamood ◽  
Michael San Francisco ◽  
Abdul N. Hamood
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sigma Factor ◽  
Regulatory Gene ◽  
Maximum Level ◽  
Pcr Analysis ◽  
Exotoxin A ◽  
Rt Pcr ◽  
Iron Starvation ◽  
The Relationship

Pseudomonas aeruginosa exotoxin A (ETA) production depends on the virulence-factor regulator Vfr. Recent evidence indicates that the P. aeruginosa iron-starvation sigma factor PvdS also enhances ETA production through the ETA-regulatory gene regA. Mutants defective in vfr, regA and pvdS, plasmids that overexpress these genes individually and lacZ transcriptional/translational fusion plasmids were utilized to examine the relationship between vfr, regA and pvdS in regulating P. aeruginosa ETA production. ETA concentration and regA expression were reduced significantly in PAOΔvfr, but pvdS expression was not affected. Overexpression of Vfr produced a limited increase in ETA production in PAOΔpvdS, but not PAOΔregA. Additionally, overexpression of either RegA or PvdS did not enhance ETA production in PAOΔvfr. RT-PCR analysis showed that iron did not affect the accumulation of vfr mRNA in PAO1. These results suggest that: (i) Vfr enhances toxA expression in PAO1 both directly and indirectly through regA, but not through pvdS; (ii) vfr expression is not regulated by iron; and (iii) both Vfr and PvdS cooperate in the presence of RegA to achieve a maximum level of toxA expression.

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