FRET-Based Determination of Protein Complex Structure at Nanometer Length Scale in Living Cells

2010 ◽  
pp. 283-300
Keyword(s):  
Protein Complex ◽  
Complex Structure ◽  
Living Cells ◽  
Length Scale ◽  
Nanometer Length Scale

Mass spectrometry supported determination of protein complex structure

2013 ◽  
Vol 23 (2) ◽  
pp. 252-260 ◽  
Author(s):  
Thomas Walzthoeni ◽  
Alexander Leitner ◽  
Florian Stengel ◽  
Ruedi Aebersold
Keyword(s):  
Mass Spectrometry ◽  
Protein Complex ◽  
Complex Structure

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

Ab Initio Determination of a New Complex Structure (55 Non-Hydrogen Atoms) from Synchrotron Powder Data: An Uncommon Nickel Succinate, Ni7(C4H4O4)4(OH)6(H2O)3•7H2O

2004 ◽  
Vol 443-444 ◽  
pp. 333-336
Author(s):  
N. Guillou ◽  
C. Livage ◽  
W. van Beek ◽  
G. Férey
Keyword(s):  
Ab Initio ◽  
Complex Structure ◽  
Three Dimensional ◽  
Free Water ◽  
Hydrogen Atoms ◽  
Monoclinic System ◽  
Synchrotron Powder Diffraction ◽  
Chloride Hexahydrate ◽  
Autogenous Pressure

Ni7(C4H4O4)4(OH)6(H2O)3. 7H2O, a new layered nickel(II) succinate, was prepared hydrothermally (180°C, 48 h, autogenous pressure) from a 1:1.5:4.1:120 mixture of nickel (II) chloride hexahydrate, succinic acid, potassium hydroxide and water. It crystallizes in the monoclinic system (space group P21/c, Z = 4) with the following parameters a = 7.8597(1) Å, b = 18.8154(3)Å, c = 23.4377(4) Å,ϐ = 92.0288(9)°, and V = 3463.9(2) Å3. Its structure, which contains 55 non-hydrogen atoms, was solved ab initio from synchrotron powder diffraction data. It can be described from hybrid organic-inorganic layers, constructed from nickel oxide corrugated chains. These chains are built up from NiO6hexameric units connected via a seventh octahedron. Half of the succinates decorate the chains, and the others connect them to form the layers. The three dimensional arrangement is ensured by hydrogen bonds directly between two adjacent layers and via free water molecules.

scholarly journals Visualizing the Dynamics of Dystrophin Protein Complex Assembly in Living Cells

2005 ◽  
Vol 11 (S02) ◽  
Author(s):  
R A Draviam ◽  
B Wang ◽  
S Shand ◽  
X Xiao ◽  
S C Watkins
Keyword(s):  
Protein Complex ◽  
Living Cells ◽  
Complex Assembly ◽  
Dystrophin Protein ◽  
Protein Complex Assembly

A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

2016 ◽  
Vol 184 (2) ◽  
pp. 603-610 ◽  
Author(s):  
Man Li ◽  
Hongfang Gao ◽  
Xiaofei Wang ◽  
Yufeng Wang ◽  
Honglan Qi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticles ◽  
Tin Oxide ◽  
Living Cells ◽  
Oxide Electrode

Studies on Determination of Natural Frequencies of Industrial Turbine Blades

1995 ◽  
Author(s):  
Mahesh M. Bhat ◽  
V. Ramamurti ◽  
C. Sujatha
Keyword(s):  
Steam Turbine ◽  
Dynamic Behavior ◽  
Turbine Blade ◽  
Natural Frequencies ◽  
Complex Structure ◽  
Turbine Blades ◽  
Blade Root ◽  
Steam Turbine Blade ◽  
Manufacturing Tolerances

Abstract Steam turbine blade is a very complex structure. It has geometric complexities like variation of twist, taper, width and thickness along its length. Most of the time these variations are not uniform. Apart from these geometric complexities, the blades are coupled by means of lacing wire, lacing rod or shroud. Blades are attached to a flexible disc which contributes to the dynamic behavior of the blade. Root fixity also plays an important role in this behavior. There is a considerable variation in the frequencies of blades of newly assembled turbine and frequencies after some hours of running. Again because of manufacturing tolerances there can be some variation in the blade to blade frequencies. Determination of natural frequencies of the blade is therefore a very critical job. Problems associated with typical industrial turbine bladed discs of a 235 MW steam turbine are highlighted in this paper.

Determination of Cellular Mechanical Properties by Cell Poking, With an Application to Leukocytes

1990 ◽  
Vol 112 (3) ◽  
pp. 283-294 ◽  
Author(s):  
G. I. Zahalak ◽  
W. B. McConnaughey ◽  
E. L. Elson
Keyword(s):  
Mechanical Properties ◽  
Final Section ◽  
Mechanical Response ◽  
Living Cells ◽  
Micropipette Aspiration ◽  
Current Status ◽  
Technical Description ◽  
Analytical Results ◽  
Simple Models

In this paper we review the cell-poking technique as an approach for investigating the mechanical properties of living cells. We first summarize the rationale for the technique and the mainly qualitative results obtained so far. Then we provide a technical description of the instrument as it is configured at present. This is followed by a discussion of the current status of analytical results available for interpreting cell-poking measurements. In the final section we apply these results to an analysis of unmodulated and modulated lymphocytes and neutrophils, and conclude that the mechanical response of these leukocytes to indentation is not consistent with simple models developed by previous investigators on the basis of micropipette-aspiration experiments.

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